• 한국식품과학회지
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• 제36권6호
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• pp.851-856
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• 2004

통고추의 열발광 특성(TL)분석에서 2.5 kGy 이상의 조사 시료에서 분리된 미네랄 시료는 $150^{\circ}C$ 부근에서 TL glow curve ($TL_1$)를 나타내었고, 조사선량이 증가할수록 peak intensity가 증가하였다. 재조사(1 kGy) 방법에 의한 TL ratio($TL_1/TL_2$)는 TL 분석의 신뢰도를 높여주었다. DNA comet assay에서 비조사 시료(씨)는 전형적인 intact cell을 나타내었으나 감마선 조사 시료에서는 long tail을 가진 comet을 나타내면서 선량 의존적으로 tail length가 증가되었다. 미생물학적 방법으로써 DEFT/APC값 측정에서는 조사선량에 따라 분말고추와 통고추에서 비교적 높은 상관을 보였다. 이상의 결과에서 DNA comet assay와 DEFT/APC 방법은 방사선 조사 건고추의 screening 방법으로서, TL은 확인방법으로서 적용가능성이 확인되었다.

• 한국식품과학회지
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• 제34권1호
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• pp.18-23
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• 2002

방사선 조사된 대두의 검지방법을 연구하기 위하여 국산 및 중국산 대두에 대하여 $0.5{\sim}4.0\;kGy$의 감마선을 조사하고 열발광(TL), 전자스핀공명(ESR) 및 DNA comet 특성을 비교 검토하였다. TL 측정에서 비조사구는 $280^{\circ}C$ 부근에서 매우 낮은 glow curve를 나타내었고, 감마선 조사구는 $200^{\circ}C$ 부근에서 조사선량에 의존적인 glow curve를 나타내었다. 재조사(re-irradiation) 방법에 의한 TL ratio$(TL_1/TL_2)$의 비교는 TL 측정 결과의 정확도를 높여 주었다. 원산지별 TL 특성 비교에서 국산을 중국산에 비해 높은 TL intensity를 나타내었다(p<0.01). 대두 껍질을 사용한 ESR 측정에서는 조사시료에서 cellulose radical 유래의 특이한 signal(g = 2.02374, 1.98715)을 보여주었고 중국산 시료의 ESR signal이 더 높게 나타났다. 비조사 대두의 DNA comet은 tail이 없거나 아주 짧은 tail을 가진 전형적인 intact cell을 나타내었다. 그러나 0.5 kGy 이상의 감마선 조사 시료에서는 조사선량에 의존적으로 comet의 tail length 증가와 더불어 comet의 크기 및 농도의 변화가 일어났다. 이상의 결과에서 TL, ESR 및 DNA comet의 분석은 대두의 방사선 조사 여부 확인을 가능하게 하였으며, 원산지별 검지특성의 차이는 거의 없었다.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1076-1081
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• 2001

현재 중국으로부터 수입량이 증가하고 있는 땅콩을 대상으로 국산과 중국산 시료의 TL, ESR, DNA comet(single cell gel electrophoresis) 분석을 실시하여 원산지별 특성을 비교하였다. Density separation 방법으로 추출한 미네랄의 TL측정 결과, 감마선 조사되지 않은 시료는 25$0^{\circ}C$ 부근에서 intensity가 낮은 glow curve를 나타내었고, 조사 시료는 18$0^{\circ}C$ 부근에서 아주 강한 intensity의 glow curve를 보여주었다. 첫 번째 측정된 glow curve(TL$_1$)의 normalization을 위하여 재조사 방법에 의해 TL$_2$를 측정하여 TL ratio(TL$_1$/TL$_2$)를 비교해 본 결과, 비조사 시료는 0.05 이하, 조사 시료는 0.2이상으로 방사선 조사 여부의 판별이 가능하였다. 땅콩껍질을 사용한 ESR 측정에서는 조사 유래의 특이적인 signal이 나타나지 않아 적용 가능성이 낮았다. DNA comet assay 결과, 비조사 시료는 tail이 없거나 아주 짧은 전형적인 intact cell을 나타낸 반면, 조사 시료는 long tail을 가진 comet을 나타내면서 선량 의존적으로 (r=0.761/Korean, r=0.768/Chinese) tail length가 증가하여 조사 여부의 확인이 가능하였다. 모든 실험에서 원산지별 차이는 크지 않았다. 이상의 결과로 볼 때 땅콩의 방사선 조사 여부 확인에는 TL 분석 및 DNA comet assay가 적용 가능하였다.

• Environmental Analysis Health and Toxicology
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• 제19권1호
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• pp.101-107
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• 2004

To ascertain the effects of hair dyeing application on the DNA damage in human lymphocytes, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp (conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp (alternative dyeing) were applied to each If young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours after the hair dyeing, and tail extent moment(TEM) and tail length (TL) were measured by using a comet assay. After dyeing, TL was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 47% and 28%, respectively, and TL for conventional dyeing group was higher than alternative dyeing group as an average of 1.2 fold. After dyeing, TEM was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 192% and 76%, respectively, and TEM for conventional dyeing group was significantly higher than alternative dyeing group as an average of 1.7 fold. Therefore, alternative dyeing application was induced to lower lymphocyte DNA damage than conventional dyeing application, and TEM was appeared to be a more sensitive tool for the measurement of lymphocyte DNA damage than TL in this study.

• 한국어류학회지
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• 제19권1호
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• pp.57-65
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• 2007

중국 상해수산대학에 소장된 어류 표본 중에 남중국해에서 채집된 1개체의 표본(295 mm TL)을 근거로 하여 홍어속(Okamejei) 어류의 신종을 기재하였다. 홍어속 어류는 북서태평양에 6종, 인도양에 3종이 서식하여 전세계에 9종이 유효하다. 호주 해역에는 홍어속의 미기재종 1종과 근연종으로 살홍어속(Dipturus)으로 소속이 변경된 3종이 서식한다. Okamejei mengae sp. nov.는 문연골 길이가 두장의 54.3%로 주둥이가 짧고, 꼬리부가 전장의 48.5%로 길고, 미극이 3열, 신경두개의 상생체공의 전단부가 명확하게 구별되는 점 등으로 홍어속에 포함된다. 이 종은 복추골이 23개로서 적고, 등지느러미 앞까지의 미추골이 50개로 많으며, 가슴지느러미의 복사연골이 96개로 많은 특징 등으로 동일속의 9종 및 근연종 등 13종과 명확히 구별된다. 또한 뾰족하게 돌출된 주둥이, 양안간격이 6.7배로 긴 두장, 넓은 꼬리, 좁은 제1등지느러미와 제2등지느러미의 거리, 짧은 제2등지느러미 뒤의 길이, 체반 등쪽에 작고 짙은 갈색 반점이 전체적으로 분포하지만 큰 반점은 없고, 배쪽의 로렌치니 병상 기관은 가슴지느러미의 후담기연골(metapterygium)의 뒤까지 분포하며, 견갑오훼연골이 높은 특징을 가지고 있다. 신종과 전 세계 홍어속 및 근연종 등 13종과의 특징 비교표를 작성하였으며, 북서태평양에 서식하는 7종에 대한 새로운 분류 검색표를 제시하였다.

• Journal of Nutrition and Health
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• 제37권9호
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• pp.794-800
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• 2004

Deer Antler has been known for its traditional oriental medicinal properties and has been widely used to promote growth, boost immune function, treat blood loss and chronic joint pain. Recent study showed imported (New Zealand) Deer Antler was beneficial in reducing the side effects of cancer treatments. However, there was no intervention study conducted on the effect of Korean Deer Antler on reducing the oxidative stress to patients with diabetes. One of the sensitive ways to measure endogenous oxidative stress is by measuring cellular DNA damage using single cell gel electrophoresis (COMET assay). This study was conducted to investigate the possible beneficial effect of commercial Deer Antler drink (provided by Chung-yang Deer Farm) on lymphocyte DNA damage and blood glucose of diabetic patients. Ten patients (4 men, 6 women) participated in the study and consumed 2 pouches of Deer Antler drink every day for 20 days. Blood was collected on the morning before and after the intervention for lymphocyte isolation and blood glucose analysis. Both systolic and diastolic blood pressure showed a tendency to decrease but did not reach statistical significance after the trial. Blood glucose level was not affected by the supplementation. After the intervention, over 50% reduction were noted in the cellular DNA damage, expressed as tail length (TL) and tail moment (TM: tail length ${\times}$ percent tail DNA) . Although we did not obtain beneficial effect on lowering blood glucose levels in the patients, this results suggest that Deer Antler may initially act in protecting endogenous DNA damage in short-term experiment.

• Journal of Electrical Engineering and Technology
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• 제8권5호
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• pp.1194-1201
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• 2013

This paper introduces a vision-based automatic system (VAMS) for non-contact measurement of morphometric characteristics of flatfish, such as total length (TL), body width (BW), height (H), and weight (W). The H and W are simply measured by a laser displacement and a load cell, respectively. The TL and BW are measured by a proposed morphological image processing algorithm. The proposed algorithm cans measurement, when the tail of flatfish is deformed, and when it is randomly oriented. In the experiment, the average and maximum measurement errors were recorded, and standard deviations and coefficients of variation (CVs) for the measurements were calculated. From those results, when flatfish the TL measurements had an average of 266.844 mm, a standard deviation of 0.351 mm, a CV of 0.131%, and a maximum error of 0.87 mm with straightened flatfish ($TL_A$ : 267 mm, $BW_A$ : 141 mm), and when flatfish of different sizes were measured, the errors in the TL and BW measurements were both about 0.2 %. Using a single conveyor, the VAMS can process up to 900 fishes per hour. Moreover, it can measure morphometric characteristics of flatfish with a TL of up to 500 mm.

• 수산해양기술연구
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• 제48권4호
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• pp.469-478
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• 2012

To manage, sort, and grade fishery resources, it is necessary to measure their morphometric characteristics. This labor-intensive task involves performing repetitive operations on land and on a research vessel. To reduce the amount of labor required, a vision-based automatic measurement system (VAMS) for the measurement of morphometric characteristics of flatfish, such as total length (TL), body width (BW), and body height (BH), has been developed as part of a database management system for fishery resources management. This system can also measure the mass (M) of flatfish. In the present study, we describe a morphological image processing algorithm for the measurement of certain characteristics of flatfish. This algorithm, which involves preprocessing, edge pattern matching, and edge point detection, is effective in cases where the flatfish being measured has a deformed tail and is randomly oriented. The satisfactory performance of the proposed algorithm is also demonstrated by means of experiments involving the measurement of the BW, TL and BH of a flatfish when it is straightened (BW : 117mm, TL : 329mm, BH : 24.5mm), when its tail is deformed, and when it is randomly oriented.

• Animal Systematics, Evolution and Diversity
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• 제37권3호
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• pp.205-211
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• 2021

This study investigates the morphological and skull characteristics of the Siberian weasel Mustela sibirica quelpartis from the Jeju Island, South Korea. A total of 26 roadkill specimens (22 males and 4 females) were collected from October 2012 to April 2016. All collected specimens were examined for morphological characteristics, but only 19 specimens (16 males and 3 females) were in a good enough condition to process for skull measurements. This study showed no significant differences in ear length between male and female (p>0.05), and significant differences were found in head-body length, tail length (TL), and hind-food length (p<0.05). Both the male and female of M. s. quelpartis were larger in its external characteristics except for TL than M. s. coreanus living in the mainland of South Korea. M. s. quelpartis males have a smaller skull size than their female counterparts. In general, both male and female M. s. quelpartis showed external characteristics in line with the Island Rule. The findings of this study are important in order to shed more light on the evolutionary mechanism of small mammals living on the Jeju Island.

• Journal of Nutrition and Health
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• 제35권2호
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• pp.213-222
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• 2002

The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.