Browse > Article

Effect of Deer Antler Drink Supplementation on Blood Pressure, Blood Glucose and Lymphocyte DNA Damage in Type 2 Diabetic Patients  

Kim Hye-Young (한남대학교 이과대학 식품영양학과)
Jeon Eun-Jae (한남대학교 이과대학 식품영양학과)
Park Yoo Kyoung (경희대학교 동서의학대학원 의학영양학과)
Kang Myung-Hee (한남대학교 이과대학 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.37, no.9, 2004 , pp. 794-800 More about this Journal
Abstract
Deer Antler has been known for its traditional oriental medicinal properties and has been widely used to promote growth, boost immune function, treat blood loss and chronic joint pain. Recent study showed imported (New Zealand) Deer Antler was beneficial in reducing the side effects of cancer treatments. However, there was no intervention study conducted on the effect of Korean Deer Antler on reducing the oxidative stress to patients with diabetes. One of the sensitive ways to measure endogenous oxidative stress is by measuring cellular DNA damage using single cell gel electrophoresis (COMET assay). This study was conducted to investigate the possible beneficial effect of commercial Deer Antler drink (provided by Chung-yang Deer Farm) on lymphocyte DNA damage and blood glucose of diabetic patients. Ten patients (4 men, 6 women) participated in the study and consumed 2 pouches of Deer Antler drink every day for 20 days. Blood was collected on the morning before and after the intervention for lymphocyte isolation and blood glucose analysis. Both systolic and diastolic blood pressure showed a tendency to decrease but did not reach statistical significance after the trial. Blood glucose level was not affected by the supplementation. After the intervention, over 50% reduction were noted in the cellular DNA damage, expressed as tail length (TL) and tail moment (TM: tail length ${\times}$ percent tail DNA) . Although we did not obtain beneficial effect on lowering blood glucose levels in the patients, this results suggest that Deer Antler may initially act in protecting endogenous DNA damage in short-term experiment.
Keywords
deer antler drink; type 2 diabetes; oxidative DNA damage; tail length; DNA in tail; tail moment;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 19: 257-267, 1996   DOI   ScienceOn
2 Cameron NE, Cotter MA, Maxfield EK. Antioxidant treatment prevents the development of peripheral nerve dysfunction in streptozotocindiabetic rats. Diabetologia 36: 299-304, 1993   DOI   ScienceOn
3 Sun SJ, Chung HW, Han JH. Smoking related DNA damage in human lymphocytes assessed by the comet assay. Envioronmental Mutagens & Carcinogens 22 (2) : 83-89, 2002
4 Leinonen J, Lehtimaki T, Toyokuni S, Okada K, Tanaka T, Hiai H, Ochi H, Laippala P, Rantalaiho V, Wirta O, Pasternack A, Alho H. New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus. FEBS Lett 417: 150-152, 1997   DOI   ScienceOn
5 Dincer Y, Akcay T, Alademir Z, Ilkova H, Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes, Mutat Res 505: 75-81, 2002
6 Pathak NN, Pattanaik AK, Patra RC. Arora BM. Mineral composition of antlers of three deer species reared in captivity. Small Ruminant Res 42: 61-65, 2001   DOI   ScienceOn
7 Kim KH, Park SW. A Study on the Hemopoietic Action of Deer Horn Extract. JBMB 15 (2): 151-157, 1982
8 Kim NJ. Effect of Deer Horn Extract to Escherichia coli Antibody Production. Korean Journal of Oriental Medicine 6: 119-132, 1985
9 Lee TH, Lee HI. Histological Study on the effect of Deer Horn to Starved-Rat Thyroid Glands. Kyunghee Univ Oriental Med J 7: 249-259, 1984
10 Jane E, Wayne HF, Jim J. Effect of supplementation with tomate juice, vitamin E, and vitamin C on LDL oxidation and products of inflammatory activity in type 2 diabetes. Diabetes Care 23: 733-738, 2000   DOI   ScienceOn
11 Park WY, Yun YP. Effect of Aloe vera Treatment on Blood Glucose Level and Clinical Chemistry in Diabetic Patients. J Fd Hyg Safety 10(1): 13-17, 1995
12 Reaven P. Dietary and pharmacologic regimens to reduce lipid peroxidation in non-insulin-dependent diabetes mellitus. Am J Clin Nutr 62(6) : 1483-1489, 1995
13 Park YK, Park EJ, Kim JS, Kang MH. Daily grape juice consumption reduces oxidative DNA damage and plasma free radical levels in healthy Koreans. Mutat Res 529: 77-86, 2003   DOI
14 Sardas S, Yilmaz M, Oztok U, Cakir N, Karakaya AE. Assessment of DNA strand breakage by comet assay in diabetic patients and the role of antioxidant supplementation. Mutat Res 490: 123-129, 2001   DOI
15 Lean ME, Noroozi M, Kelly I, Burns J, Talwar D, Sattar N, Crozier A. Dietary Flavonols Protect Diabetic Human Lymphocytes Against Oxidative Damage to DNA. Diabetes 48: 176-181, 1999   DOI   ScienceOn
16 How K, Choi SH, Lee HB, Chung KC, Ko DR. Studies on Deer Horn: 2. The Effect of Deer Horn on the growth in Experimental Rats. J Korean Pharm Sci 5 (1): 10, 1990
17 Bae DS. Studies on the Effects of Velvet on Growth of Animals: 3. Effects of velvet on the growth of internal organs and blood picture of chicken. Chungbuk Univ J 10: 209-216, 1972
18 Choi DY, Shin MK, Lee SI, Lee HI, Kim WH. The Effect of Deer Horn on the Experimental Liver damage in Rats. Kyunghee Univ Oriental Med J 2: 43-51, 1979
19 Alan JS. Free radical mechanisms and vascular complications of diabetes mellitus. Diabetes Rev 2: 7-10, 1993
20 Singh PN, McCoy MT, Tice RR Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191, 1988   DOI   ScienceOn
21 Sampson MJ, Astley S, Richardson T, Willis G, Davies IR, Hughes DA, Southon S. Increased DNA oxidative susceptibility without increased plasma LDL oxidizability in Type II diabetes: effects of alpha-tocopherol supplementation. Clin Sci 101 (3) : 235-241, 2001   DOI   ScienceOn
22 Pitozzi V, Giovannelli L, Bardini G, Rotella CM, Dolara P. Oxidative DNA Damage in peripheral blood cells in type 2 diabetes mellitus: higher vulnerability of polymorphonuclear leukocytes. Mutat Res 529: 129-133, 2003   DOI
23 Hannon-Fletcher MPA, O'Kane MJ, Moles KW, Weatherup C, Barnett CR, Barnett YA. Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects. Mutat Res 460: 53-60, 2000   DOI
24 Jeon EJ, Park YK, Kang MH. The Effect of Deer Antler Mixtures on the oxidative DNA damage in Human lymphocyte cell. Annual Spring Convention ofthe Korean Nutrition Society, 2002
25 Cho HC, Kim MJ, Suh JC, Cho YH, An KW, Jung CH, Park CK Lee SI, Bae HY, Lee BL. The Activity of Erythrocyte Antioxidant Enzymes According to Glucose Concentration in Non Insulin Dependent. Diabetes Mellitus. Diabetes 18: 337-343, 1994
26 Yong JI. Effect of Deer Horn on Serum Cholesterol Level in Cholesterol fed Rabbits. J Korean Pharm Sci 5 (6): 6-9, 1960
27 Ostling O, Johanson KJ. Microgel electrophoretic study of radiation- induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123: 291-298, 1984   DOI   ScienceOn
28 Yong JI. Studies on Deer Horn: The Effect of Deer Horn on the Liver and Other Organs of Cholesterol Administered Rabbits. J Korean Pharm Sci 8 (1): 12-29, 1964
29 Shin KH, Lim SS, Chung HS, Baek IB. Analysis of the Composition of Biochemical Component in Unossified Antlers. Kor J Pharmacogn 30: 314-319, 1999
30 Fuller JH, Stevens LK. Epidemiology of hypertension in diabetic patients and implications for treatment. Diabetes Care 14: 8-12, 1991   DOI   ScienceOn
31 Janjic D, Andereggen E, Deng S. Improved insulin secretion of cryopreserved human islet by antioxidant treatment. Pancreas 13: 166-172, 1996   DOI   ScienceOn
32 Cho MR, Choue RW, Chung SH, Ryu JW. Effects of Silkworm Powder on Blood Glucose and Lipid Levels in NIDDM (Type II) Patients. Korean J Nutr 31 (7) : 1139-1150, 1998
33 Shin MK, Lee SI, Kim WH, Ahn BK, Lee HI. Effect of Deer Horn on the Bone Marrow Iron of Experimental Anemia Rats. Kyunghee Univ Oriental Med J 2: 69-72, 1979
34 Paolisso G, D'Amore A, Galzerano D, Balbi V, Giugliano D, Varricchio M, D'Onofrio F. Daily Vitamin E supplements improve metabolic control but insulin secretion in elderly type II diabetic patients. Diabetes Care 16(11) : 1433-1437, 1993   DOI   ScienceOn
35 Astley S, Langrish-Smith A, Southon S, Sampson M. Vitamin E supplementation and oxidative damage to DNA and plasma LDL in type 1 diabetes. Diabetes Care 22 (10): 1626-1631, 1999   DOI   ScienceOn
36 Sunwoo HH, Nakano T, Hudson RJ and Sim JS. Chemical Composition of Antlers from wapiti (Cervus elaphus). J Agric Food Chem 43 : 2846-2849, 1995   DOI
37 Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy- 2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res 387: 147-163, 1997   DOI   ScienceOn
38 Choi MS, Do DH, Choi DJ. The Effect of Mixing Beverage with Aralia continentalis Kitagawa Root on Blood Pressure and Blood Constituents of the Diabetic and Hypertensive Elderly. Korean J. Food & Nutr 15 (2): 165-172, 2002
39 Han SH. The Effect of Deer Horn on stressed (Starve, Thermal, Cold condition) Rats: enterochromaffin-like cell. Catholic Univ Oriental Med J 19: 157-166, 1970
40 Ceriello A, Russo P D, Amstad P, Cerutti P. High glucose induces antioxidant enzymes in human endothelial cells in culture. Diabetes 45: 471-477, 1996   DOI   ScienceOn
41 Photo Collection of Estimated Food Weight. Korean Dietetic Assoc, 1999
42 Wolff SP. Diabetes mellitus and free radicals. Br Med Bull 49: 642-652, 1993