• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.851-856
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• 2004

Minerals separated from irradiated dried red pepper (whole) at 2.5 kGy or higher showed typical thermoluminescence (TL) glow curves ($TL_1$) at around $150^{\circ}C$, which increased with irradiation dose. The TL ratio ($TL_1/TL_2$) through re-irradiation step at 1 kGy enhanced reliability of TL identification results. DNA comet assay indicated that the intact cell was observed in non-irradiated pepper (seed), while some long tails were found in irradiated ones, showing relationship between irradiation dose and tail length. Log DEPT/APC values increased in proportion to irradiation doses in powdered and whole peppers. Based on overall results, irradiated dried red peppers could be screened using DNA comet assay or log DEFT/APC, and moreover the identification results were verified by TL analysis.

• Korean Journal of Food Science and Technology
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• v.34 no.1
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• pp.18-23
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• 2002

The detection characteristics of gamma-irradiated $(0{\sim}4\;kGy)$ soybeans produced in Korea and China were investigated by thermoluminescene (TL), electron spin resonance (ESR), and DNA comet assay. The TL glow curves were shown at around $200^{\circ}C$ for irradiated soybeans, while that at $280^{\circ}C$ for the non-irradiated one. The normalization with a re-irradiation step at 1 kGy could verify the above detection results. The Korean soybean showed higher glow curves than Chinese did. The ESR spectroscopy for husks of irradiated soybeans revealed specific signals (g = 2.02374, 1.98715) derived from cellulose radical, which intensities were proportional to irradiation does, with the higher peaks in Chinese sample than Korean one. The DNA comet for the non-irradiated sample showed no or little tails, while those for irradiated samples above 0.5 kGy were remarkably changed in their length, size, and concentration, thus resulting in distinguishing non-irradiated from irradiated samples. As a result, TL, ESR, and DNA comet determinations were found suitable for the detection of irradiated soybean at 0.5 kGy or more, and negligible differences were observed between Korean and Chinese origins in their detection characteristics.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1076-1081
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• 2001

Gamma-irradiated peanuts, Korean and Chinese origins, were investigated on detection properties by thermoluminescence (TL), electron spin resonance (ESR), and DNA comet assay (single cell gel electrophoresis). TL measurement showed that the non-irradiated sample revealed a glow curve with low intensity at about 25$0^{\circ}C$, while the irradiated samples showed higher intensity around at 18$0^{\circ}C$. TL ratio (TL$_1$/TL$_2$) of area for TL$_1$ glow curve to TL$_2$ was below 0.05 for the non-irradiated sample and 0.2 or more for the irradiated ones, thus identifying each other. ESR spectroscopy for the irradiated peanuts using outer skin showed negligible signals induced by irradiation, indicating ESR is little applicable to the detection of irradiated peanuts. In DNA comet assay, the non-sample had no or very short tails, whereas the irradiated samples revealed the cells with long tails. Significance in the increase of their lengths depending on irradiation dose (r=0.761/Korean, r=0.768/Chinese) was also found. There was no remarkable difference in detection properties by origins of samples in all determinations, It is concluded that TL analysis or DNA comet assay is suitable for detection of irradiated peanuts and a combined method is recommendable for enhancing the reliability of detection results.

• Environmental Analysis Health and Toxicology
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• v.19 no.1
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• pp.101-107
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• 2004

To ascertain the effects of hair dyeing application on the DNA damage in human lymphocytes, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp (conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp (alternative dyeing) were applied to each If young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours after the hair dyeing, and tail extent moment(TEM) and tail length (TL) were measured by using a comet assay. After dyeing, TL was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 47% and 28%, respectively, and TL for conventional dyeing group was higher than alternative dyeing group as an average of 1.2 fold. After dyeing, TEM was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 192% and 76%, respectively, and TEM for conventional dyeing group was significantly higher than alternative dyeing group as an average of 1.7 fold. Therefore, alternative dyeing application was induced to lower lymphocyte DNA damage than conventional dyeing application, and TEM was appeared to be a more sensitive tool for the measurement of lymphocyte DNA damage than TL in this study.

• Korean Journal of Ichthyology
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• v.19 no.1
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• pp.57-65
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• 2007

A new species of the rajid genus Okamejei is described from a single specimen (295 mm TL) from off Shantou, Gwangdong in the South China Sea. The new species differs from all other congeners in the following combination of characters: snout pointed, dorsal head length 6.7 times interorbital width, tail moderately wide and long, its length 48.5% TL, interdorsal distance less than length of first dorsal fin base, postdorsal tail short as 5.8% TL, small evenly distributed dark brownish spots, without ocelli on dorsal surface of disc, pores of ampullae of Lorenzini on ventral surface distributed from snout tip to distal end of metapterygium, scapulocoracoid high, its height about 1.4 times rear corner height, trunk vertebrae 23, predorsal tail vertebrae 50 and pectoral fin radials 96.

• Journal of Nutrition and Health
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• v.37 no.9
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• pp.794-800
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• 2004

Deer Antler has been known for its traditional oriental medicinal properties and has been widely used to promote growth, boost immune function, treat blood loss and chronic joint pain. Recent study showed imported (New Zealand) Deer Antler was beneficial in reducing the side effects of cancer treatments. However, there was no intervention study conducted on the effect of Korean Deer Antler on reducing the oxidative stress to patients with diabetes. One of the sensitive ways to measure endogenous oxidative stress is by measuring cellular DNA damage using single cell gel electrophoresis (COMET assay). This study was conducted to investigate the possible beneficial effect of commercial Deer Antler drink (provided by Chung-yang Deer Farm) on lymphocyte DNA damage and blood glucose of diabetic patients. Ten patients (4 men, 6 women) participated in the study and consumed 2 pouches of Deer Antler drink every day for 20 days. Blood was collected on the morning before and after the intervention for lymphocyte isolation and blood glucose analysis. Both systolic and diastolic blood pressure showed a tendency to decrease but did not reach statistical significance after the trial. Blood glucose level was not affected by the supplementation. After the intervention, over 50% reduction were noted in the cellular DNA damage, expressed as tail length (TL) and tail moment (TM: tail length ${\times}$ percent tail DNA) . Although we did not obtain beneficial effect on lowering blood glucose levels in the patients, this results suggest that Deer Antler may initially act in protecting endogenous DNA damage in short-term experiment.

• Journal of Electrical Engineering and Technology
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• v.8 no.5
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• pp.1194-1201
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• 2013

This paper introduces a vision-based automatic system (VAMS) for non-contact measurement of morphometric characteristics of flatfish, such as total length (TL), body width (BW), height (H), and weight (W). The H and W are simply measured by a laser displacement and a load cell, respectively. The TL and BW are measured by a proposed morphological image processing algorithm. The proposed algorithm cans measurement, when the tail of flatfish is deformed, and when it is randomly oriented. In the experiment, the average and maximum measurement errors were recorded, and standard deviations and coefficients of variation (CVs) for the measurements were calculated. From those results, when flatfish the TL measurements had an average of 266.844 mm, a standard deviation of 0.351 mm, a CV of 0.131%, and a maximum error of 0.87 mm with straightened flatfish ($TL_A$ : 267 mm, $BW_A$ : 141 mm), and when flatfish of different sizes were measured, the errors in the TL and BW measurements were both about 0.2 %. Using a single conveyor, the VAMS can process up to 900 fishes per hour. Moreover, it can measure morphometric characteristics of flatfish with a TL of up to 500 mm.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.4
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• pp.469-478
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• 2012

To manage, sort, and grade fishery resources, it is necessary to measure their morphometric characteristics. This labor-intensive task involves performing repetitive operations on land and on a research vessel. To reduce the amount of labor required, a vision-based automatic measurement system (VAMS) for the measurement of morphometric characteristics of flatfish, such as total length (TL), body width (BW), and body height (BH), has been developed as part of a database management system for fishery resources management. This system can also measure the mass (M) of flatfish. In the present study, we describe a morphological image processing algorithm for the measurement of certain characteristics of flatfish. This algorithm, which involves preprocessing, edge pattern matching, and edge point detection, is effective in cases where the flatfish being measured has a deformed tail and is randomly oriented. The satisfactory performance of the proposed algorithm is also demonstrated by means of experiments involving the measurement of the BW, TL and BH of a flatfish when it is straightened (BW : 117mm, TL : 329mm, BH : 24.5mm), when its tail is deformed, and when it is randomly oriented.

• Animal Systematics, Evolution and Diversity
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• v.37 no.3
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• pp.205-211
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• 2021

This study investigates the morphological and skull characteristics of the Siberian weasel Mustela sibirica quelpartis from the Jeju Island, South Korea. A total of 26 roadkill specimens (22 males and 4 females) were collected from October 2012 to April 2016. All collected specimens were examined for morphological characteristics, but only 19 specimens (16 males and 3 females) were in a good enough condition to process for skull measurements. This study showed no significant differences in ear length between male and female (p>0.05), and significant differences were found in head-body length, tail length (TL), and hind-food length (p<0.05). Both the male and female of M. s. quelpartis were larger in its external characteristics except for TL than M. s. coreanus living in the mainland of South Korea. M. s. quelpartis males have a smaller skull size than their female counterparts. In general, both male and female M. s. quelpartis showed external characteristics in line with the Island Rule. The findings of this study are important in order to shed more light on the evolutionary mechanism of small mammals living on the Jeju Island.

• Journal of Nutrition and Health
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• v.35 no.2
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• pp.213-222
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• 2002

The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.