• Archives of Pharmacal Research
• /
• 제27권4호
• /
• pp.422-428
• /
• 2004

Expressed sequence tags (ESTs) were generated from two 3'-directed CDNA libraries constructed from quiescent and activated rat hepatic stellate cell (HSC) to analyze the expression profiles of active genes in both cells. From quiescent and activated HSC, 694 ESTs and 779 ESTs, respectively, were obtained after excluding those having shorter than 30 bp. Amonq ESTs obtained from quiescent and activated HSC, 68 and 73 kinds of ESTs (186 clones and 236 clones), respectively, appeared more than once, implying that their genes are expressed highly in each cell type. 52 among 73 ESTs appeared only in the activated HSC 47 amonq 68 ESTs only in the normal HSC, and 21 in both cells. The genes of these 52 ESTs were assumed to be expressed more highly in the activated HSC. To confirm the high expression of genes of which the ESTs appeared more than twice in the activated HSC, northern hybridization was carried out with RNAs derived from rat normal and fibrotic liver using each of 18 EST DNAs as probe. 13 ESTs showed more intense bands with RNA isolated from the fibrotic liver than normal liver. From these results, we confirm the positive correlation between abundance of transcript in activated HSCs and the expression level in fibrotic liver, The expression profile of the transcripts serves as an important tool in understanding the biological properties of HSC.

• International Journal of Concrete Structures and Materials
• /
• 제18권1E호
• /
• pp.29-34
• /
• 2006

Elastic modulus, tensile and bond capacities are important factors for developing an effective reinforcing action of a flexural member as a reinforcing material for concrete structures. Reinforcement must have enough bond capacity to prevent the relative slip between concrete and reinforcement. This paper presents an experimental study to clarify the bond capacity of prestressed carbon fiber reinforced polymer(CFRP) rod manufactured by an automatic assembly robot. The bond characteristics of CFRP rods with different pitch of helical wrapping were analyzed experimentally. As the result, all types of CFRP rods show a high initial stiffness and good ductility. The mechanical properties of helical wrapping of the CFRP rods have an important effect on the bond of these rods to concrete after the bond stress reached the yield point. The stress-slip relationship analyzed from the pull-out test of embedded cables within concrete was linear up to maximum bond capacity. The deformation within the range of maximum force seems very low and was reached after approximately 1 mm. The average bond capacity of CF20, CF30 and CF40 was about 12.06 MPa, 12.68 MPa and 12.30 MPa, respectively. It was found that helical wrapping was sufficient to yield bond strengths comparable to that of steel bars.

• 자연과학논문집
• /
• 제17권1호
• /
• pp.55-64
• /
• 2006

이 연구에서는 6개의 히스티딘이 첨부된 파지 K11 라이소자임의 제조 분리 및 특성을 알아보고자 하였다. 첨부된 히스티딘에 의한 이 효소의 활성도의 변화는 없었으며, 효소 활성의 최적 pH는 7.2-7.4 이었다. 여러 다른 종류의 양이온 존재하의 활성도를 측정한 결과 칼슘과 마그네슘 이온에 의해 효소 활성이 완전히 억제되었으나, 아연이나 나트륨 이온은 효소의 활성도를 이 이온들이 없을 때와 같은 정도로 유지하였다. 단지 100 mM 이상의 아연 이온 농도에서 K11 라이소자임 효소 활성도를 완전히 억제하였다.

• 한국미생물·생명공학회지
• /
• 제17권4호
• /
• pp.295-300
• /
• 1989

B. thuringiensis가 생산하는 살충성 독소 단백질의 생태학적 응용방법을 개발하기 위한 목적으로 우선 독소 단백질 유전자를 옮겨 발현시키기에 적합한 숙주 미생물의 분리작업을 수행하였다. 국내 주요농산물인 고추, 감자, 무우 등 7가지 농작물의 뿌리부근에 군락을 형성하는 35종의 형광성Pseudomonas들을 분리하였고 독소 단백질 유전자를 함유하는 재조합 plasmid에 대한 숙주로서의 이응가능성을 검토해 보기 위하여 분리균주 35주에 대한 형질전환을 실시한 결과 4주에 독소 단백질 유전자의 도입이 가능하였고 생물검정과 면역학적인 방법 등에 의한 결과 BT 독소 유전자의 발현을 확인하였다.

• 한국IT서비스학회지
• /
• 제12권1호
• /
• pp.271-287
• /
• 2013

The rapid growth and dissemination of touch-based mobile devices such as smart phones and tablet PCs, gives numerous benefits to people using a variety of multimedia contents. Due to its portability, it enables users to watch a soccer game, search video from YouTube, and sometimes tag on contents on the road. However, the limited screen size of mobile devices and touch-based character input methods based on this, are still major problems of searching and tagging multimedia contents. In this paper, we propose WalkieTagging, which provides a much more intuitive way than that of previous one. Just like any other previous video tagging services, WalkieTagging, as a voice-based annotation service, supports inserting detailed annotation data including start time, duration, tags, with little effort of users. To evaluate our methods, we developed the Android-based WalkieTagging application and performed user study via a two-week. Through our experiments by a total of 46 people, we observed that experiment participator think our system is more convenient and useful than that of touch-based one. Consequently, we found out that voice-based annotation methods can provide users with much convenience and satisfaction than that of touch-based methods in the mobile environments.

• Journal of Applied Biological Chemistry
• /
• 제42권1호
• /
• pp.7-11
• /
• 1999

An extracellular chitinase of the selected strong antifungal microorganism, Serratia sp. 3095, was purified by salting out, affinity adsorption, Sepadex G-100 gel fitration, Sepadex G-75 gel fitration and DEAE Sepadex A-50 chromatography. The molecular weight of the purified chitinase was estimated to be 62,000 dalton by SDS-PAGE. Optimal pH and temperature of the chitinase were pH 7.5 and 45, respectively. The enzyme retained more than 80% of the activity between pH 5.5 and pH 10.5, and below $50^{\circ}C$ but was unstable above $60^{\circ}C$, below pH 5.0. The activity of the chitinase was inhibited about 60% by $Sn^{2+}$, 40% by $Hg^{2+}$ and $Ag^+$, 70% by AHA, 40% by iodoacetate, 35% by thiourea and p-CMB, but stabilized by SDS. $K_m$ value of the purified chitinase was 3.68 mg/ml for colloidal chitin. The chitinase from Serratia sp. 3095 showed antifungal activity to Fusariurm solani.

• 한국콘텐츠학회논문지
• /
• 제9권8호
• /
• pp.84-90
• /
• 2009

본 논문에서는 장치장에서 대역폭의 낭비없이 실시간으로 경로 복구를 효율적으로 하기 위한 방안으로 RFID태그를 YT의 위치파악에 사용하는 RFID기반의 ad-hoc 네트워크 구축방안을 제시한다. 장치장 내 YT와 GC 및 YT와 TC간 ad-hoc 통신을 통해 중앙 서버의 통제 없이 양 적하 작업을 수행하여, 실시간 적인 작업 대응으로 장치장의 효율적 운영이 가능하도록 한다. RFID 태그의 위치정보를 이용하여 컨테이너 터미널의 장치장 환경에 적합한 라우팅 프로토콜을 제안하고, 네트워크 시뮬레이터를 통한 성능평가를 수행하여 성능이 우수함을 확인했다.

• 한국컴퓨터산업학회논문지
• /
• 제6권5호
• /
• pp.821-828
• /
• 2005

This paper was proposed Intelligent and wireless USN/RFID module system that can overcome disadvantage of existing RFID system with no sensing module and wire communication. The proposed USN/RFID system was designed and fabricated. After fabricating new system, we analyzed the characteristics of USN/RFID module. After design VCO block that is point circuit to develop next generation system one chip of RFID system. we were carried out simulation and verified the validity, this paper was showed that VCO system was enough usable in wireless network module. USN/RFID Reader module shows superior result that validity awareness distance corresponds to 30M in the case of USN and to 5M in RFID Reader's case and 900MHz of commercial frequency does practical use enoughly in range of high frequency. The USN/RFID Reader module is considered to act big role to Ubiqitous industry offering computing surrounding of new concept that is intelligence type service and that was associated to real time location system(RTLS). environment improvement/supervision. national defense, traffic administration etc.

• Korean Journal of Acupuncture
• /
• 제25권1호
• /
• pp.165-196
• /
• 2008

Objective : The purpose of this study is to observe the effects of herbal-acupuncture with Acanthopanacis cortex extract (AC-HA) at KI10 (Eumgok) on osteoporosis in ovariectomized (OVX) ddy mice. Methods : We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum creatinine, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : Tibial BMD was significantly decreased by OVX, while significantly increased by AC-HA at KI10. Serum creatinine level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Serum osteocalcin level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Tibial TBV(trabecular bone volume) was significantly decreased by OVX, while significantly increased by AC-HA at KI10. AC-HA at KI10 significantly suppressed the OVX-induced increase of GPL(growth plate length) of tibia in mice. Conclusion : These results suggest that AC-HA at KI10 has a therapeutic effect on osteoporosis in OVX mice.

• 한국어병학회지
• /
• 제27권2호
• /
• pp.127-131
• /
• 2014

넙치에 적합한 표지방법을 조사하기 위하여 표지 후 누적폐사율, 혈액생화학적 성상과 조직병리학적 조사를 하였다. 본 연구에서는 아가미뚜껑 절단, 배지느러미 50%와 100% 절단 방법을 사용하였다. 누적폐사율은 아가미뚜껑 절단구가 가장 높았다. 배지느러미 100% 절단구와 아가미뚜껑 절단구는 절단 후 13일 째 조직병리학적 변화를 나타내었다: 간에서 화농성 염증반응, 체신에서 섬유성 염증반응이 관찰되었다. 본 연구결과 50% 배지느러미 절단구가 가장 효과적인 표지방법인 것으로 판단된다.