• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.140-145
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• 2007

This study was carried out to develop effective test method by soybean stink bug and to screen resistant genetic resources against soybean stink bug. The damage pod rate by stink bug showed 40% of most soybean varieties and was selected about 10% low of 10 varieties by 298 variety and degree in soybean at first year Stink bug damage rate research for 102 varieties that stink bug damage rate lowed at first year showed 10% low of 12 varieties and from 20% to 30% of the other varieties. So testing material is "Ilpumgeumjeongkong" to develop for effective test method soybean stink bug and result for stink bug damage rate research of according to growth stage showed rapidly high more full seed than full pod. Full seed stage (R6) was highest to 35.5% for stink bug damage rate. Result of resistant genetic resources selection according to stink bug damage pod rate was lowed of best to 10.3% for "Peking, Sorogkong, Hwangsaegjunjeari and Sobaeknamulkong" in the order. Also, stink bug damage seed rate was similar too. So "Peking, Sorogkong, Hwangsaegjunjeari and Sobaeknamulkong" were thought resistant variety against stink bug. Additional study carried out with "Peking and Sorogkong" so that concretely investigate about stink bug's refuse reaction. This result showed 10.0% for Peking and 14.2% for Sorogkong at R6 stage. But, damage pod rate was rapidly lowed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.2
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• pp.508-516
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• 2010

An autonomous and automatic home mess-cleanup robot is newly developed in this paper. Thus far, vacuum-cleaners have lightened the burden of household chores but the operational labor that vacuum-cleaners entail has been very severe. Recently, a cleaning robot was commercialized to solve but it also was not successful because it still had the problem of mess-cleanup, which pertained to the clean-up of large trash and the arrangement of newspapers, clothes, etc. Hence, we develop a new home mess-cleanup robot (McBot) to completely overcome this problem. The robot needs the capability for agile navigation and a novel manipulation system for mess-cleanup. The autonomous navigational system has to be controlled for the full scanning of the living room and for the precise tracking of the desired path. It must be also be able to recognize the absolute position and orientation of itself and to distinguish the messed object that is to be cleaned up from obstacles that should merely be avoided. The manipulator, which is not needed in a vacuum-cleaning robot, has the functions of distinguishing the large trash that is to be cleaned from the messed objects that are to be arranged. It needs to use its discretion with regard to the form of the messed objects and to properly carry these objects to the destination. In particular, in this paper, we describe our approach for achieving accurate localization using RFID for home mess-cleanup robots. Finally, the effectiveness of the developed McBot is confirmed through live tests of the mess-cleanup task.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.87-94
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• 2008

Porcine epidemic diarrhea virus (PEDV) is an infectious and highly contagious virus of swine. In order to develop the transgenic tobacco culture cells producing PEDV antigen protein, four vectors expressing PEDV spike protein (SP) gene under the control of a CaMV 35S promoter were constructed. Four fragments of the SP region of PEDV, SP1 (444 bp, 1487-1930 bp), SP2 (1.7 kb, 2300-3987 bp), SP3 (1.4 kb, 1559-2950 bp), and SP4 (2.6 kb, 9-2643 bp) were amplified by PCR and then C-MYC tag was fused to the end of each SP gene, respectively. These cassettes are inserted into the pCAMBIA2300 (named as 35S::SP1-M, 35S::SP2-M 35S::SP3-M, and 35S::SP4-M, respectively). Tobacco (cv. BY-2) cultured cells were transformed by co-cultivation with Agrobacterium tumefaciens harboring expression vector. We selected kanamycin-resistant calli and checked for the presence of the introduced SP gene using PCR, resulting 70% of them showed the foreign gene. We selected the lines with high-level expression of PEDV antigen protein based on dot blot analysis. Southern blot analysis confirmed that the PEDV SP gene was integrated into the genome of the tobacco cultured cells. Northern blot analysis showed that the introduced gene was highly expressed in transgenic cultured cells. Transgenic tobacco cultured cells-derived antigen induced immunogenicity in mice as determined by a plaque reduction neutralization assay. These results suggest that the vectors expressing PEDV spike protein gene in this study will be useful for the development of transgenic plants and cultured cells producing PEDV antigene protein.

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.153-160
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• 2009

Pisidium (Neopisidium) coreanum is a freshwater snail and lives in spring water near mountain areas. Interestingly, this snail has been traditionally regarded as medicinal food, and thus has been used as folk remedies for healing broken bones. Recently, alpha classification on Pisidium (Neopisidium) coreanum through redescription has been conducted. However, not much attention has been made in beta classification. In this study, we performed the beta classification based on metallothionein (MT) genes found from various organisms. To this end, the complete cDNA sequences were obtained from the Expressed Sequence Tag (EST) sequencing project of Pisidium (Neopisidium) coreanum. The coding region (315 bp) encoded an amino acid sequence of 105 residues. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Pc-MT gene indicate that Pisidium (Neopisidium) coreanum has similarity to freshwater bivalve such as Dreissena polymorpha (zebra mussel), Unio tumidus (swollen river mussel) and Crassostrea ariakensis (suminoe oyster).

• Journal of Energy Engineering
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• v.18 no.4
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• pp.221-229
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• 2009

This study develops the optimized operation program which enables effective and economic operation between individual and connected branch offices by analyzing the current status and influential indicators of district heating companies' capital branch offices. Accordingly, the study examines the efficiency of optimized operation program. In doing so, this study has diagnosed and analyzed various factors, such as boilers, pumps, and relevant tags (temperature, pressure, fuel amount) through investigation of individual branch offices, and finally succeeded in developing wide-ranging data base by factor covering one-year time period. Additionally, after running the optimized operation program, different branch offices, optimum preference has turned out "incinerator receiving heat from KEPCO>CHP >PLBs>PLBw." Meantime, except the connected offices, there has been no big difference between actual and optimum operation program in branch offices. Meanwhile, the integrated optimum operation program has made it possible the most optimal result only via the connecting supply and demand heat without changing received Heat from KEPCO which is the same as total productive heat. The result has showed that the reduction percentage per day is 2.45~6.80%, and the reduction cost per day is 22,727~60,077 thousand won given the randomly selected sample days. In particular, winter time shows the highest demand with the largest reduction cost whereas summer time illustrates the lowest demand with the smallest reduction cost. Given this result, reduction cost per year compared to actual heat production cost for one year theoretically would be 84 hundred million won. Also, the economic effect showed that the reduction cost percentage per year is more than 2.74% on heat production cost per year for all capital branch offices.

• Journal of Internet Computing and Services
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• v.23 no.5
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• pp.145-154
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• 2022

Automatic Target Recognition (ATR) technology is emerging as a core technology of Future Combat Systems (FCS). Conventional ATR is performed based on IMINT (image information) collected from the SAR sensor, and various image-based deep learning models are used. However, with the development of IT and sensing technology, even though data/information related to ATR is expanding to HUMINT (human information) and SIGINT (signal information), ATR still contains image oriented IMINT data only is being used. In complex and diversified battlefield situations, it is difficult to guarantee high-level ATR accuracy and generalization performance with image data alone. Therefore, we propose a knowledge graph-based ATR method that can utilize image and text data simultaneously in this paper. The main idea of the knowledge graph and deep model-based ATR method is to convert the ATR image and text into graphs according to the characteristics of each data, align it to the knowledge graph, and connect the heterogeneous ATR data through the knowledge graph. In order to convert the ATR image into a graph, an object-tag graph consisting of object tags as nodes is generated from the image by using the pre-trained image object recognition model and the vocabulary of the knowledge graph. On the other hand, the ATR text uses the pre-trained language model, TF-IDF, co-occurrence word graph, and the vocabulary of knowledge graph to generate a word graph composed of nodes with key vocabulary for the ATR. The generated two types of graphs are connected to the knowledge graph using the entity alignment model for improvement of the ATR performance from images and texts. To prove the superiority of the proposed method, 227 documents from web documents and 61,714 RDF triples from dbpedia were collected, and comparison experiments were performed on precision, recall, and f1-score in a perspective of the entity alignment..

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.366-374
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• 2009

To determine the optimum operating conditions of the indented cylinder length grader based on varietal characteristics, broken rice removal capabilities were measured on the milled rice of 41 varieties at different conditions consisted of 3 types of indented cylinders with different indent-opening diameters and 3 different collecting-angles of broken rice. The broken rice removal capabilities were swayed by the indent's opening diameter and depth as well as the angle of the collecting trough of broken rice on the point of instrument, and by the kernel length and width as well as 1000 grain weight of milled rice on the point of rice variety. When the angle of broken rice collecting-trough reached to near the horizontal center line of the indented cylinder on the direction of upward turning side, which was referred $0^{\circ}$ in this paper, the amount of rice collected in trough increased, whereas the loss of head rice also increased. Considering the removal rate of broken rice as well as loss of head rice, it was thought that the suitable angle of trough for broken rice collecting was located $5^{\circ}$ to $15^{\circ}$ depending on varietal characteristic and indent opening diameter. It was thought that 4.2 mm or more of indent opening diameter was recommendable for the rice varieties having heavier 1000 grain weight than 22.3g, as well as larger sizes than 2.9 and 5.2 mm in width and length of rice kernel, respectively; 3.8 mm for the small-sized thin kernels, and a proper diameter between 3.8 and 4.2 mm for short to middle kernels. The varieties with relatively shorter length compared to width of kernel were more difficult to separate the broken rice than the opposite ones. For effective separation of that, it seems that some specific indent shapes such as wider opening and shallow depth etc. are required. When the broken rice content were excessively high, wider diameters of indent openings than specified sizes were thought to be the better.

• Journal of Life Science
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• v.21 no.7
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• pp.1032-1038
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• 2011

Recently identified Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP) consists of an ion channel-like transmembrane domain (VSD) and a phosphatase-like domain. Ci-VSP senses the change of membrane potential by its VSD and works as a phosphoinositide phosphatase by its phosphatase domain. In this study, we present the construction of His-tagged phosphatase-like domain of Ci-VSP, its recombinant expression and purification, and its enzymatic activity behavior in order to examine the biochemical behavior of phosphatase domain of Ci-VSP without interference. We found that Ci-VSP(248-576)-His can be eluted with an elution buffer containing 25 mM NaCl and 100 mM imidazole during His-tag purification. In addition, we found the proper measurement condition for kinetics study of Ci-VSP(248-576)-His against p-nitrophenyl phosphate (pNPP). We measured the kinetic constant of Ci-VSP(248-576)-His at $37^{\circ}C$, pH 5.0 or 5.5, under 30 min of reaction time, and less than $2.0\;{\mu}g$ of protein amount. With these conditions, we acquired that Ci-VSP(248-576)-His has $K_m$ of $354{\pm}0.143\;{\mu}M$, $V_{max}$ of $0.0607{\pm}0.0137\;{\mu}mol$/min/mg and $k_{cat}$ of $0.359{\pm}0.009751\;min^{-1}$ for pNPP dephosphorylation. Therefore, we produced a pure form of Ci-VSP(248-576)-His, and this showed a higher activity against pNPP. This purified protein will provide the road to a structural investigation on an interesting protein, Ci-VSP.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.9 no.1
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• pp.31-38
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• 2006

Purpose: Infantile constipation is one of the most common problems in pediatric gastrointestinal outpatient clinic. We planed to show the clinical feature of infantile constipation and explore the possible relationship between diets and symptoms of constipation. Methods: We analyzed the medical records and telephone questionnaire about infants under 2 years of age with constipation, who visited outpatient clinic of Department of Pediatrics, Ilsan Paik Hospital during the time from March 2002 to February 2005. Data including the symptoms and signs of constipation, diet history, and past-medical history were analyzed. Results: Total 96 infants, 40 male and 56 female, were enrolled in this study. The mean age was 9 months. Twenty-three infants were exclusively breast milk fed (BMF), 20 infants were cow's milk fed (CMF) and 53 infants had a history of mixed feeding with cow's milk and breast milk (MMF). Patients showed painful defecation (95.8%), abdominal distension (53.1%), palpable rectal stool (35.1%), hard stool (30.2%), blood-tinged stool (29.2%) and anal fissure (16.7%). Patients with exclusive cow milk feeding had prominent clinical features, such as anal fissure (p=0.03), hematochezia (p=0.04) and palpable rectal stool (p=0.025). Patients who had a history of larger intake of liquid food had a tendency to get anal skin tag (p<0.05). Conclusion: Exclusive breast milk feeding seemed important to avoid constipation with clinical significance. To educate caregivers in appropriate way of the weaning food may help the infants with constipation.

• Journal of Animal Science and Technology
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• v.55 no.5
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• pp.399-403
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• 2013

This study was conducted to investigate the basic concept of multiple trait effective daughter contribution (MTEDC) for dairy cattle sires and calculate effective daughter contribution (EDC) by applying a five lactation multiple trait model using milk yield test records of daughters for the Multiple-trait Across Country Evaluation (MACE). Milk yield data and pedigree information of 301,551 cows that were the progeny of 2,046 Korean and imported dairy bulls were collected from the National Agricultural Cooperative Federation and used in this study. For MTEDC approximation, the reliability of the breeding value was separated based on parents average, own yield deviation and mate adjusted progeny contribution. EDC was then calculated by lactation using these reliabilities. The average number of recorded daughters per sire by lactations were 140.57, 94.24, 55.14, 29.20 and 14.06 from the first to fifth lactation, respectively. However, the average EDC per sire by lactation using the five lactation multiple trait model was 113.49, 89.28, 73.56, 54.02 and 35.08 from the first to fifth lactation, respectively, while the decrease of EDC in late lactations was comparably lower than the average number of recorded daughters per sire. These findings indicate that the availability of daughters without late lactation records is increased by genetic correlation using the multiple trait model. Owing to the relatedness between the EDC and reliability of the estimated breeding value for sire, understanding the MTEDC algorithm and continuous monitoring of EDC is required for correct MACE application of the five lactation multiple trait model.