• Journal of Plant Biotechnology
• /
• v.45 no.4
• /
• pp.322-327
• /
• 2018

Post-translational modification of proteins regulates signaling cascades in eukaryotic system, including plants. Among these modifications, phosphorylation plays an important role in modulating the functional properties of proteins. Plants perceive environmental cues that directly affect the phosphorylation status of many target proteins. To determine the effect of environmentally induced phosphorylation in plants, in vivo methods must be developed. Various in vitro methods are available but, unlike in animals, there is no optimized methodology for detecting protein phosphorylation in planta. Therefore, in this study, a robust, and easy to handle Phos-Tag Mobility Shift Assay (PTMSA) is developed for the in vivo detection of protein phosphorylation in plants by empirical optimization of methods previously developed for animals. Initially, the detection of the phosphorylation status of target proteins using protocols directly adapted from animals failed. Therefore, we optimized the steps in the protocol, from protein migration to the transfer of proteins to PVDF membrane. Supplementing the electrophoresis running buffer with 5mM $NaHSO_3$ solved most of the problems in protein migration and transfer. The optimization of a fast and robust protocol that efficiently detects the phosphorylation status of plant proteins was successful. This protocol will be a valuable tool for plant scientists interested in the study of protein phosphorylation.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
• /
• 2007.04a
• /
• pp.447-450
• /
• 2007

Positioning technology for moving object is an important and essential component of ubiquitous. Also RFID(Radio Frequency IDentification) is a core technology of ubiquitous wireless communication. In this study we adapted kalman-filter theory to RFID-based Positioning System in order to trace a time-variant moving object and verify the positioning accuracy using RMSE (Roong technology for moving object is an important and essential component of ubiquitous Mean Square Error). The purpose of this study is to verify an effect of kalman-filter on the positioning accuracy and to analyze what does each design factor have an effect on the positioning accuracy by means of simulations and to suggest a standard of optimal design factor of a RFID-based Positioning System. From the results of simulations, Kalman-filer improved the positioning accuracy remarkably; the detection range of RFID tag is not a determining factor. The smaller standard deviation of detection range improves the positioning accuracy. However it accompanies a smaller fluctuation of the positioning accuracy. The larger detection rate of RFID tag yields the smaller fluctuation in the positioning accuracy and has more stable system and improves the positioning accuracy;

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
• /
• 2008.10a
• /
• pp.297-302
• /
• 2008

The positioning technology for a moving object is an important and essential component of ubiquitous computing environment and applications, for which Radio Frequency Identification(RFID) has been considered as a core technology. RFID-based positioning system calculates the position of moving object based on k-nearest neighbor(k-nn) algorithm using detected k-tags which have known coordinates and kcan be determined according to the detection range of RFID system. In this paper, RFID-based positioning system determines the position of moving object not using weight factor which depends on received signal strength but assuming that tags within the detection range always operate and have same weight value. Because the latter system is much more economical than the former one. The geometries of tags were determined with considerations in huge buildings like office buildings, shopping malls and warehouses, so they were determined as the line in I-Dimensional space, the square in 2-Dimensional space. In 1-Dimensional space, the optimal detection range is determined as 125% of the tag spacing distance through the analytical and numerical approach. Here, the analytical approach means a mathematical proof and the numerical approach means a simulation using matlab. But the analytical approach is very difficult in 2-Dimensional space, so through the numerical approach, the optimal detection range is determined as 134% of the tag spacing distance in 2-Dimensional space. This result can be used as a fundamental study for designing RFID-based positioning system.

• Journal of KIISE
• /
• v.43 no.10
• /
• pp.1173-1178
• /
• 2016

Since the introduction of BitTorrent protocol in 2001, everything can be downloaded through file sharing, including music, movies and software. As a result, the copyright holder suffers from illegal sharing of copyright content. In order to solve this problem, countries have enacted illegal share related law; and internet service providers block pirate sites. However, illegal sites such as pirate bay easily reopen the site by changing the domain name. Thus, we propose a technique to easily detect pirate sites that are reopened. This automated technique collects the domain names using the google search engine, and measures similarity using Longest Common Subsequence (LCS) algorithm by comparing the tag structure of the source web page and reopened web page. For evaluation, we colledted 2,383 domains from google search. Experimental results indicated detection of a total of 44 pirate sites for collected domains when applying LCS algorithm. In addition, this technique detected 23 pirate sites for 805 domains when applied to foreign pirate sites. This experiment facilitated easy detection of the reopened pirate sites using an automated detection system.

• Proceedings of the Korean Institute of Communication Sciences Conference
• /
• 2006.07a
• /
• pp.362-362
• /
• 2006

• Proceedings of the Korean Institute of Communication Sciences Conference
• /
• 2006.07a
• /
• pp.350-350
• /
• 2006

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.34 no.12A
• /
• pp.925-932
• /
• 2009

Precise temperature monitoring is the major preconditioning to supervise quality losses within the transport chain for fresh products. ISO/IEC18000-6REV1 defines new protocols supporting BAP(Battery Assisted Passive) RFID tag which is completely compatible with EPCglobal Class1 Generation2 specification. In this paper, we designed a modem supporting BAP RFID tag with FPGA(Field Programmable Gate Array) and implemented sensor data processing function defined in ISO/IEC18000-6REV1. The transmit block of the modem supports pulse shaping filter and the output signal of the implemented RFID reader is satisfied with the spectrum mask defined in the standard. The receive block of the modem uses Gardner TED to synchronize timing of symbol. In this paper, we designed a modem supporting ISO/IEC18000-6REV1 standard and developed a RFID reader sndard. The developed RFID reader sndard can recognize sensor tag and passive tag in the wireless environment and supports real-time processing of the sensor data in the embedded linux platform.

• Journal of Korea Multimedia Society
• /
• v.20 no.3
• /
• pp.474-481
• /
• 2017

In Korea, foot-and-mouth disease has not been reported for several decades, but it began to develop again in 2000. For 2010~2011, when the worst occurred, 3.5 million animals were disposed of resulting in a loss of 2.8 trillion won. In order to prevent the harmful effects of foot-and-mouth disease, vaccination and housing management are being implemented. Despite these measures, foot-and-mouth disease is infected with air through the respiratory tract and accompanies fever after latency. Therefore, it is recognized that measuring and managing the body temperature of livestock at the early stage is the first step of managing this disease. In this paper, we propose a temperature monitoring system that can measure the body temperature by incorporating temperature sensor mounted in ear-tag of cattle and collect body temperature data of each individual cattle through BLE into the control server. The proposed body temperature monitoring system has various advantages such as easy installation without the help of livestock specialists and not damaging the organs of the livestock. So, it is possible to manage the abnormal symptom of cattle in real time and it is believed that the proposed monitoring system will revolutionize the prevention of foot-and-mouth disease.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.17 no.3
• /
• pp.747-754
• /
• 2013

This paper proposed a new transponder detection method to reduce tag recognition time and simplify anti-collision process in RFID systems. In conventional systems, a transponder in detectable area is passively recognized by responding to a reader command. In addition, if there are multiple tags in the area, a collision between tags occurs by responding at the same time to a command and anti-collision process begins. In the proposed method, tags are actively recognized without any command from a reader which results to reducing a tag recognition time and simplifying anti-collision process. To compare transponder recognition time, the process of transponder recognition in conventional systems is analysed. A circuit to detect transponder directly is developed and antenna voltage variation of a reader in the appearance of multiple transponders is analysed for the implementation of the proposed method.

• Journal of Chest Surgery
• /
• v.32 no.2
• /
• pp.151-157
• /
• 1999

Background: Increasing evidences from experimental studies indicate that apoptosis may be inversely related to angiogenesis in tumor progression. Material and Method: To explore how apoptosis correlates with tumor angiogenesis, we measured the apoptotic index(AI) using the terminal deoxynucleotidyl transferase method(Apop Tag In Situ Apoptosis Detection Kit, ONCOR) and the intratumoral microvessel density using the anti-CD31 monoclonal antibody in non-small cell lung cancer. Result: Statistical analysis revealed an inverse correlation between AIs and intratumoral microvessel densities in squamous cell lung carcinoma(Spearman rank correlation coefficient r=- 0.229, p=0.047). Conclusion: The results of this study demonstrated that the amount of apoptosis in squamous cell lung carcinoma may be influenced by the extent of neovascularization. This suggests that tumor angiogenesis may contribute to a reduction of apoptosis in tumor cells.