• Journal of dental hygiene science
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• v.17 no.5
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• pp.433-438
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• 2017

Relative to its incidence, oral cancer has serious negative social effects. The exact causes of oral cancer have not been clarified, but many studies have implicated smoking and drinking. However, the fundamental mechanism of oral cancer causation has yet to be elucidated. Lysophosphatidic acid (LPA) augments epithelial mesenchymal transition (EMT) and development of various cancer cells. However, a detailed mechanistic explanation for LPA-induced EMT and the effects of EMT-promoting conditions on oral squamous cell carcinoma development remain elusive. In the present study, a quantitative reverse transcription polymerase chain reaction was used to analyze TWIST1, Slug, E-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript expression. Immunoblotting was used to analyze TWIST1, Slug, E-cadherin, and GAPDH protein expression. siRNAs were used to silence TWIST1 and Slug transcript expression. A matrigel-coated in vitro invasion insert was used to analyze oral cancer cell invasion. The results of the present study show that the expression levels of TWIST1 and Slug, which are EMT factors, were increased by LPA treatment in YD-10B oral squamous cell carcinoma. Conversely, E-cadherin expression was significantly reduced. In addition, transfection of the cells with TWIST1 and Slug siRNA strongly inhibited LPA-induced oral cancer cell invasion. The present study shows that TWIST1 and Slug mediate LPA-induced oral cancer cell EMT and invasiveness. The present study confirmed the mechanism by which LPA promotes oral cancer cell development, with TWIST1 and Slug providing novel biomarkers and promising therapeutic targets for oral cancer cell development.

• Biomedical Science Letters
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• v.24 no.2
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• pp.76-86
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• 2018

Breast cancer is one of the most common cancers affecting women worldwide. Although the survival rate of breast cancer has increased, breast cancer still results in a high mortality rate. Breast cancer deaths are caused by metastasis that occurs in organ dysfunction. Recently, there have been many studies on circulating tumor cells (CTCs), which are related to breast cancer metastasis in the blood. Recent studies have demonstrated that some CTCs do not express epithelial markers. Therefore, in this study, total RNA was extracted from blood without separating out the CTCs, and the characteristics of the CTCs were analyzed by RT-qPCR. Cyclin D1 and twist-related protein 1 (TWIST1) are well-known markers for predicting the prognosis of patients with breast cancer. However, few studies have demonstrated the use of CCND1 and TWIST1 in blood as diagnostic and prognostic markers of breast cancer. In this study, patients with late-stage breast cancer had overexpressed CCND1 and TWIST1 than patients with different stages of breast cancer (P < 0.001 and P < 0.01, respectively). The relative expression level of CCND1 in survivors was higher than in patients who died (P = 0.06). The relative expression level of TWIST1 in survivors was lower than in patients who died (P = 0.08). Overall CCND1 and TWIST1 were not useful as markers for the diagnosis of breast cancer through blood. However, we showed the possibility of using CCND1 and TWIST1 as prognostic markers, and a large-scale study is needed to confirm the usefulness of these prognostic markers.

• Fibers and Polymers
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• v.7 no.1
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• pp.79-84
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• 2006

This paper deals with the grey fabric deformation during relaxation. It investigates the effect of twist direction and twist liveliness on the 2/2 twill weave woven fabric distortion, during relaxation This experimental work shows that twist liveliness increases the natural tendency of the twill weave woven fabric to skew. In addition when the twist direction of the warp and weft is opposite to each other the bias curling occurs too.

• Journal of Genetic Medicine
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• v.8 no.2
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• pp.130-134
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• 2011

Saethre-Chotzen syndrome is an autosomal dominant craniosynostosis syndrome, usually involving unior bilateral coronal synostosis and mild limb deformities, and is induced by loss-of-function mutations of the TWIST1 gene. Other clinical features of this syndrome include ptosis, low-set ears, hearing loss, hypertelorism, broad great toes, clinodactyly, and syndactyly. The authors of the present study report 2 children with clinical features of Saethre-Chotzen syndrome who showed mutations in the TWIST1 gene, and is the first molecular genetic confirmation of Saethre-Chotzen syndrome in Korea. The molecular genetic testing of the TWIST1 gene for patients with coronal synostoses is important to confirm the diagnosis and to provide adequate genetic counseling.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.8
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• pp.3797-3800
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• 2014

Background: BMI1, TWIST1 and SNAI2/SLUG have been implicated in aggressive behavior of squamous cell carcinoma (SCC) and melanoma and BMI1 expression could identify subtypes of Merkel cell carcinoma (MCC). However, BMI1, TWIST1 and SNAI2 expression levels in basal cell carcinomas (BCCs) have not been elucidated. We hypothesized BCC could be a good model system to decipher mechanisms which inhibit processes that drive tumor metastasis. The aim of this study was to examine the mRNA expression level of BMI1, TWIST1, and SNAI2 in BCCs. Materials and Methods: Thirty-five fresh non-metastatic BCC tissue samples and seven fresh normal skin tissue samples were evaluated by real-time RT-PCR. Results: BMI1 and TWIST1 demonstrated marked down-regulation (p<0.00l, p=0.00l respectively), but SNAI2 showed no significant change (p=0.12). Conclusions: Previous literature has clearly demonstrated a positive association between BMI1 and TWIST1 expression and metastatic BCC, aggressive SCC and melanoma. Here, we demonstrated a negative association between BMI1 and TWIST1 mRNA expression level and BCC.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.1
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• pp.243-252
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• 2015

The present study, to examine the effect of the exercise for weight control, compares the difference of the heart rate and energy expenditure by sex during Twist exercise. Twenty eight adults (male 14, female 14) were carried out Twist exercise for 7 minutes and 14 minutes. During performing the exercise program, to perform HR and related variables were measured for 7 minutes and 14 minutes. Through a twist exercise treatment and the following results were obtained. 1. Females had slightly higher, without statistical significance, number of heart beat, minimum heart rate, maximum heart rate, and average heart rate than male had for both 7 minutes and 14 minutes of Twist exercise. 2. In the case of energy consumption, significant differences did not appear until 7 minutes to perform Twist exercise. On the other hand, the energy consumption for 14 minutes of performance was significantly higher in male than in female (p<.05). However, energy expenditure for the period per minute per unit weight (kcal/min/kg) showed no difference. 3. If more than 30 minutes duration were preformed the Twist exercise, males were expected to consume significantly higher than females (p<.05). To consume calories for the 10,000 step walk of, it would be required about 76 minutes for Twist exercise time without distinction of sex. Through the above findings, twist exercise is a form of aerobic exercise for obesity prevention and weight control, as well as it might be beneficial to practice and maintain the health and physical fitness with offering a variety of exercise opportunities in the public.

• The Research Journal of the Costume Culture
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• v.21 no.1
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• pp.1-16
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• 2013

This study analyzed the characteristics of Korean traditional plain weave excavated from Sang-go period to Joseon Dynasty. To do this, this study classified the Korean traditional plain weave into fiber types(cotton, hemp & ramie, plain weaved silk), analyzed and compared the thickness, twist type of yarns and density by times. First, in characteristics of cotton, the average and maximum density of Joseon Dynasty were higher than those of Goryeo, twist type was mainly s-twist and the density of warp was higher than that of weft. Second, the maximum density of hemp & ramie was found in era of Three Kingdoms of Korea. In common characteristics of hemp & ramie, twist type was mainly s-twist(sometimes non-twist) to the Three Kingdoms of Korea and was changed into non-twist from the Goryeo. The density of warp was higher than that of weft in common. Generally, the average density of ramie was higher than that of hemp. Third, in the characteristics of plain weaved silk, twist type was mainly non-twist(sometimes s and z-twist) from Sang-go period to Joseon Dynasty. Warp-faced ribbed tabby was excavated in Goryeo, the average density of warp-faced ribbed tabby was higher than that of other fiber types plain weave. Generally, in all fiber types, the density of warp was higher than that of weft.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.669-672
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• 2013

Objective: To investigate the expression of Twist and E-cadherin in ovarian cancer tissues as well as the role of epithelial-mesenchymal transformation (EMT) in ovarian cancer metastasis. Method: The expressions of Twist and E-cadherin in 54 cases of ovarian cancer and paracancerous tissues were detected by Western blottin g and reverse transcriptase polymerase chain reaction. We used RNA interference to silence Twist expression in human ovarian cancer cell line, and detected E-cadherin expression using Western blotting. Results: There was an increase in the relative abundance of Twist proteins and a decrease in E-cadherin in ovarian cancer compared with normal ovary tissues (P < 0.05). The expression levels of Twist and E-cadherin mRNA were $1.49{\pm}0.53$ and $0.82{\pm}0.24$ in ovarian cancer, and $1.14{\pm}0.38$ and $1.08{\pm}0.19$ in paracancerous tissues, respectively. The difference between the indicators in ovarian cancer and in paracancerous tissues was statistically significant (P < 0.05). When the Twist expression was silenced in an ovarian cancer cell line, the expression of the E-cadherin protein increased (P<0.05). Conclusion: The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis.

• Textile Coloration and Finishing
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• v.23 no.1
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• pp.35-42
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• 2011

For high quality DTP products, it is important to optimize the parameters of media, pre- and after-treatment, ink, printer, etc. This study investigated the effect of types of fabrics(media) as a DTP parameters. Especially, the effects of media properties such as yarn twist and knitted fabric structure, on printability and color difference were examined. Two types of cotton yarn twist(830 and 1630 twist/meter) and five knitted structures of media were prepared with a single circular knitting machine. The K/S values of hard-twist samples were higher than those of normal-twist samples in every media structures. It is more effective to use the knitted fabrics of a hard-twist yarn to obtain dark color in the printing above input level value 60 where the printability improvement was most pronounced in case of plain structure. Among the five media structures a plain structure was the highest and that of corduroy was the lowest in terms of K/S values. Also ${\Delta}E$ values and lightness of the hard-twist yarn samples were smaller than that of normal-twist yarn samples when ${\Delta}E$ values were tested by using a standard of a normal twist yarn sample with a plain structure, which was increased in the case of corduroy structure.

• Textile Coloration and Finishing
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• v.20 no.1
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• pp.36-43
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• 2008

False twist texturing uses the thermoplastic property of synthetic yarn and produces a bulk and a stretch by giving the filament a permanent crimp. This study investigated the effects of false twist texturing parameters, disk/yarn(D/Y) and heater temperature, on the physical properties of nylon textured yarn. D/Y was a primary determinant of twist numbers of filaments and was proportionally increased with the twist numbers of filaments, resulting in an increase of crimp rigidity of the textured yarn. Heater temperature, a determinant of the heat setting of transformed filaments, was proportionally increased with the increase of crimp rigidity. Nylon 6 was partially melted at $190^{\circ}C$. This study consequently suggested the optimal parameters of nylon 6 85d/68f full-dull POY.