Browse > Article
http://dx.doi.org/10.7314/APJCP.2013.14.2.669

Expression and Significance of Twist and E-cadherin in Ovarian Cancer Tissues  

Wang, Wen-Shuang (Department of Gynecology, Yantai Yuhuangding Hospital)
Yu, Shou-Li (Department of Gynecology, Yantai Yuhuangding Hospital)
Yang, Xing-Sheng (Department of Gynecology, Qilu Hospial of Shandong University)
Chang, Shu-De (Department of Gynecology, Yantai Affiliated Hospital of Binzhou Medical College)
Hou, Jian-Qing (Department of Gynecology, Yantai Yuhuangding Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 669-672 More about this Journal
Abstract
Objective: To investigate the expression of Twist and E-cadherin in ovarian cancer tissues as well as the role of epithelial-mesenchymal transformation (EMT) in ovarian cancer metastasis. Method: The expressions of Twist and E-cadherin in 54 cases of ovarian cancer and paracancerous tissues were detected by Western blottin g and reverse transcriptase polymerase chain reaction. We used RNA interference to silence Twist expression in human ovarian cancer cell line, and detected E-cadherin expression using Western blotting. Results: There was an increase in the relative abundance of Twist proteins and a decrease in E-cadherin in ovarian cancer compared with normal ovary tissues (P < 0.05). The expression levels of Twist and E-cadherin mRNA were $1.49{\pm}0.53$ and $0.82{\pm}0.24$ in ovarian cancer, and $1.14{\pm}0.38$ and $1.08{\pm}0.19$ in paracancerous tissues, respectively. The difference between the indicators in ovarian cancer and in paracancerous tissues was statistically significant (P < 0.05). When the Twist expression was silenced in an ovarian cancer cell line, the expression of the E-cadherin protein increased (P<0.05). Conclusion: The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis.
Keywords
Twist; E-cadherin; ovarian cancer; epithelial-mesenchymal transition;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brasch J, Harrison OJ, Honig B, Shapiro L (2012). Thinking outside the cell: how cadherins drive adhesion. Trends Cell Biol, 22, 299-310.   DOI   ScienceOn
2 Cho EY, Choi Y, Chae SW, Sohn JH, Ahn GH (2006). Immunohistochemical study of the expression of adhesion molecules in ovarian serous neoplasms. Pathol Int, 56, 62-70.   DOI   ScienceOn
3 Dara E, Scoazec JY, Walker-Combrouze F, et al (1997). Expression of cadherinsin benign, borderline, and malignant ovarian epithelial tumors: a clinicopathologic study of 60 cases. Hum Pathol, 28, 922-8.   DOI   ScienceOn
4 De Beco S, Amblard F, Coscoy S (2012). New insights into the regulation of E-cadherin distribution by endocytosis. Int Rev Cell Mol Biol, 95, 63-108.
5 De Wever O, Westbroek W, Verloes A, et al (2004). Critical role of N-cadherin in myofibroblast ivasion and migration in vitro stimulated by colon-cancer- cell-derived TGF-$\beta$or wounding. J Cell Sci, 117, 4691-703.   DOI   ScienceOn
6 Geho DH, Bandle RW, Clair T, Litta LA (2005). Physiological mechanisms of tumor-cell invasion and migration. Physiology, 20, 194-200.   DOI   ScienceOn
7 Hosono S, Kajiyama H, Terauchi M, et al (2007). Expression of Twist in creases the risk for recurrence and for poor survival in epithelial ovarian carcinoma patients. Br J Cancer, 96, 314-20.   DOI   ScienceOn
8 Kajiyama H, Kikkawa F, Khin E, et al (2003). Dipeptidyl peptidase IV overexpression induces up-regulation of E-cadherin and tissue inhibitors of matrix metalloproteinases, resulting in decreased invasive potential in ovarian carcinoma cells. Cancer Res, 63, 2278-83.
9 Kang Y, Massague J (2004). Epithelial-mesenchymal transitions: twist in development and metastasis. Cell, 118, 277-9.   DOI   ScienceOn
10 Kim KJ, Jang SJ, Yu E (2008). Loss of E-cadherin and cytoplasmic-nuclear expression of b -catenin are the most useful immunoprofiles in the diagnosis of solid-pseudopapillary neoplasm of the pancreas. Hum Pathol, 39, 251-8.   DOI   ScienceOn
11 Kwok WK, Ling MT, Lee TW, et al (2005). Up-regulation of Twist in prostate cancer and its implication as a therapeutic target. Cancer Res, 65, 5153-62.   DOI   ScienceOn
12 Lander R, Nordin K, Labonne C (2011) . The F-box protein Ppa is a common regulator of core EMT factors Twist, Snail, Slug, and Sip1. J Cell Biol, 194, 17-25.   DOI   ScienceOn
13 Myong NH (2012). Loss of E-cadherin and Acquisition of Vimentin in Epithelial-Mesenchymal Transition are Noble Indicators of Uterine Cervix Cancer Progression. Korean J Pathol, 46, 341-8.   DOI   ScienceOn
14 Larue L, Bellacosa A (2005). Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 3' kinase /AKT pathways. Oncogene, 24, 7443-54.   DOI   ScienceOn
15 Lee JM, Dedhar S, Kalluri R, Thompson EW (2006). The epithelial-mesenchymal transition: new insights in signaling, development and disease. Cell Biol, 172, 973-81.   DOI   ScienceOn
16 Lee TK, Poon RT, Yuen AP, et al (2006). Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition. Clin Cancer Res, 12, 5369-76.   DOI   ScienceOn
17 Niu RF, Zhang L, Xi GM, et al (2007). Up-regulation of Twist induces angiogenesis and correlates with metastasis in hepatocellular carcinoma. J Exp Clin Cancer Res, 26, 385-94.
18 Puisieux A (2009). Role of epithelial-mesenchymal transition in tumor progression. Bull Acad Natl Med, 193, 2017-34.
19 Qin Q, Xu Y, He T, Qin C, Xu J ( 2012). Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms. Cell Res, 22, 90-106.   DOI
20 Radisky DC (2005). Epithelial-mesenchymal transition. J Cell Sci, 118, 4325-6.   DOI   ScienceOn
21 Thiery JP (2002). Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer, 2, 442-54.   DOI   ScienceOn
22 Thompson EW, Newgreen DF (2005). Carcinoma invasion and metastasis: a role for epithelial-mesenchymal transition? Cancer Res, 65, 5991-5.
23 Zhang Z, Xie D, Li X, et al (2007). Significance of Twist expression and its association with E-cadherin in bladder cancer. Hum Pathol, 38, 598-606.   DOI   ScienceOn
24 Yang J, Mani SA, Dongaher JL, et al (2004). Twist,a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell, 117, 927-39.   DOI   ScienceOn
25 Yoshida J, Hofiuchi A, Kikuchi N, et al (2009). Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression. Med Mol Morphol, 42, 82-91.   DOI