• KSBB Journal
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• 제11권6호
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• pp.704-711
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• 1996

경제성분석 기능을 가진 생물공정 flowsheeting 소프트웨어인 BioPro Designer를 이용하여 외부단 백질 생산 시스템의 최적 공정설계가 수행되었다. BioPro Designer를 이용하여 Salmonella phage P P22의 tailspike protein의 생산시스템에 적용되었 는데 최적공정을 선택하는 과정에서 투입되는 시간 과 노력에 획기척인 개선이 있었다. 숙주의 내부에 서 생산되는 외부 단백질의 folding 특성이 가장좋 은 생산시스템을 선택하는데 영향을 미 친다는 것을 보여주기위하여 wild type의 tailspike와 두개의 다른 변종 tailspike들, 즉 tsf G244→R과 Su A334 -V을 비교 검토되었다. 회수된 tailspike 단백질의 단위질량 대비 자본투자비를 비교 척도로 하여 최척 의 tailspike 생산 시스템이 선택되었다.

• The Plant Pathology Journal
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• 제23권3호
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• pp.143-150
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• 2007

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2009년도 추계학술대회 논문집
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• pp.403-404
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• 2009

• Advances in pediatric surgery
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• 제4권2호
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• pp.125-130
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• 1998

To assess the clinical and nutritional status of long-term survivors of biliary atresia, history taking, medical record review, physical examination (height, weight, MAC, TSF), blood tests (LFT, prothrombin time, platelet count, prealbumin, calcium) and liver needle biopsy were performed in 12 patients in whom Kasai procedure were performed more than 10 years ago at Department of Pediatric Surgery in Seoul National University Hospital. None were below the 5th percentile in height and weight. TSF was above the 75th percentile in all patients and showed good subcutaneous fat deposition. MAC was above the 5th percentile in all patients. Serum prealbumin level was abnormal in 2 patients with abnormal liver function and revealed visceral protein malnutrition. Serum calcium level was decreased below normal range in 4 patients with abnormal liver function. One patient had mild ascites. Five patients had abnormal liver function and 7 patients showed clinical manifestation of portal hypertension. Liver needle biopsy was performed in 5 patients and no cirrhotic change was observed. Although some patients who have survived for more than 10 years after Kasai procedure developed protein malnutrition and vitamin deficiencies, growth and development and nutritional status were generally satisfactory. Five patients(42%) showed normal liver function and no portal hypertension. In conclusion, Kasai procedure is satisfactory as a primary treatment in biliary atresia but significant portion of long-term survivors had abnormal liver function and portal hypertension. Continuous and careful follow-up is necessary to determine when liver transplantation may be indicated.

• Journal of Nutrition and Health
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• 제36권4호
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• pp.397-404
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• 2003

The present study was designed to compare the nutritional status of hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients. Subjects were 58 HD patients (male/female = 29/29) and 33 CAPD patients (male/female = 23/10) undergoing dialysis treatment in Artificial Kidney Unit of Chonbuk National University Hospital. For nutritional assessment, %IBW (ideal body weight), %TSF (tricep skin fold), %MAC (mid arm circumference), %MAMC (mid arm muscle circumference), serum albumin, serum transferrin, TLC (total lymphocyte count), SGA (subjective global assessment) and estimated energy and protein intakes by 1-month food frequency method were used. Between HD and CAPD group, mean age (50 $\pm$ 12 vs. 52 $\pm$ 12 yr), dialysis durations (37 $\pm$ 36 vs. 30 $\pm$ 26 mon), dietary energy intakes (28.3 $\pm$ 9.0 vs. 28.8 $\pm$ 8.6 kcal/kg/day), dietary protein intakes (1.1 $\pm$ 0.4 vs. 1.2 $\pm$ 0.3 g/kg/day) and incidence of co-morbid conditions (69.0% vs. 69.7%) were not significantly different. Data by using SGA showed a higher incidence of malnutrition in CAPD patients (45.6%) than in HD patients (36.2%). %IBW (p < 0.001), %TSF (p < 0.001) and %MAC (p < 0.001) were higher in CAPD patients than were in HD patients. But serum albumin (p < 0.001) and transferrin (p < 0.001) were significantly lower in CAPD patients than were in HD patients. A higher incidence of malnutrition was shown in CAPD Patients than in HD Patients due to different dialysis type. A significant finding was that CAPD showed protein deficient malnutrition and HD did calorie deficient malnutrition. It suggests that an adequate dietary intake considering dialysis type prevents a prevalence of malnutrition.

• 한국임상약학회지
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• 제5권2호
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• pp.51-60
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• 1995

Obesity is defined as excessive storage of energy in the form of fat resulting in adverse effect on healthy and longevity. Some data have shown increased incidence of hypertension, diabetes, hypercholesterolemia in overweight individuals and other disorders such as joint disease, cancer have been also associated with obesity. Recently, various commercial diet programs have been introduced for weight reduction without scientific evaluation for clinical effect and safety. The diet program must be based on a sound and scientific rationale and also be safe and nutritionally adequate. Pulmuone $diet^{(R)}$ is a calorie-restricted commercial diet-food, which contains adequate nutritients. The efficacy and safety of Pulmuone diet were evaluated in 28 normal healthy obese females in a prospective study. Each volunteer was placed in 3-week diet program. To observe weight reduction effect, body weight, TSF and AMC were measured every week. To account for safety of this program, blood chemistry test, blood pressure and side effects were monitored every week. Mean body weight was reduced by 3.24kg following diet program, which is statistically significant(P < 0.05) and this weight reduction was related to reduction in fat weight and not muscle weight as shown by the results of TSF and AMC Blood chemistry was not influenced by this program, but blood pressure was significantly reduced following this program. Thought diet program was generally well tolerated in most volunteers, the most frequent side effects are constipation, dizzness and headache. In conclusion, Pulmuone $diet^{(R)}$ program seems to be safe and effective for weight reduction in normal healthy obese females.

• 대한전자공학회논문지TC
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• 제48권4호
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• pp.37-46
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• 2011

시간동기화는 모바일 통신 시스템에서 중요한 역할을 한다. 특히, 통신 개체들 사이에 정확한 시분할 기법이 요구될 때, 네트워크 성능에 영향을 미치는 중요한 요소가 될 수 있다. 본 논문에서는 무선 모바일 애드 혹 네트워크를 위한 새로운 시간 동기화 알고리즘을 제시한다. 본 논문의 주요 목적은 레퍼런스 브로드캐스팅에서 발견된 장점을 활용하여 시간 동기화 패킷의 충돌을 줄이는데 있다. 또한 시간 동기화에 대한 수렴시간을 보장하기 위해 정교한 클럭 갱신 기법을 사용한다. 새롭게 제안한 시간 동기화 알고리즘의 성능을 평가하기 위해 모바일 애드 혹 네트워크에 대한 다양한 시나리오를 구성하고 이를 OPNET으로 구현하여 실험하였다. 컴퓨터 시뮬레이션 결과, 제안한 기법이 시간 동기화의 정확성과 수렴 시간 등의 측면에서 기존의 TSF 방식보다 좋은 성능을 나타내었다.

• Journal of Nutrition and Health
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• 제39권2호
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• pp.133-144
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• 2006

The purpose of this study was to evaluate the nutritional status and to identify related factors in elderly hemodialysis patients. Sixty-four patients who were registered in Asan Medical Center, Seoul, Korea, participated in the study. The data was collected between September and December, 2003. General characteristics were obtained with the use of an interviewer-administered questionnaire. Anthropometric and biochemical data, 3-day dietary records and dietary habits were also obtained. The results of this study were analyzed with t-test or $X^2$-test using SPSS package program. The percentage of elderly hemodialysis patients who were undergoing mild to severe malnutrition (MN group) and were normal nutrition (NN group) by subjective global assessment (SGA) criteria were 46.9% and 53.1 %, respectively. Appetite (p < 0.05) and dietary cholesterol intakes (p < 0.05) were significantly lower in MN group than the NN group. Body mass index (BMI, p < 0.001), lean body mass (LBM, p < 0.05), triceps skinfold thickness (TSF, p < 0.01), mid ann circumference (MAC, p < 0.01) and mid ann muscle circumference (MAMC, p < 0.05) were also lower in the MN group than the NN group. There were also significant differences in blood urea nitrogen (BUN, p < 0.05), creatinine (p < 0.05), prealbumin (p < 0.001) and C-reactive protein (CRP, p < 0.05). SGA was negatively correlated with serum BUN, creatinine and pre albumin, dry weight, BMI, LBM, total body water, TSF, MAC and MAMC, and positively correlated with age. In stepwise multiple regression analysis, SGA was related to BMI, CRP, age and BUN. In conclusion, almost half of the subjects were in malnourished status and had lower values in anthropometric and biochemical data. Our results suggest that SGA is a simple and adequate method for assessing the nutritional status in elderly hemodialysis patients and adequate dietary guidelines based on individual nutritional status are needed in the patients.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제26권6호
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• pp.346-354
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• 2023

Purpose: Approximately 30% of children with chronic liver disease (CLD) are malnourished. However, proper assessment of their nutritional status is difficult. The subjective global nutritional assessment (SGNA) is a comprehensive approach that uses nutrition-focused history and examination, followed by grading of malnourishment. We aimed to study the prevalence of malnutrition in children with CLD using the SGNA tool. Methods: This cross-sectional observational study included patients aged <18 years with CLD. Nutritional assessments were recorded using SGNA tool. Conventional anthropometric measurements were performed and corroborated with nutritional status using SGNA tool. Results: A total of 85 children with CLD and mean age of 62 months were enrolled in this study. The prevalence of malnourished children according to SGNA was 34%; 22% were moderately malnourished and 12% were severely malnourished. We found statistically significant differences in anthropometric parameters among the three groups. A moderate degree of agreement was found between SGNA and weight-for-age (W/A) (p=0.020), mid-upper arm circumference (MUAC) (p<0.001), and triceps skin-fold thickness (TSF)-for-age (p=0.029). Furthermore, a fair degree of agreement was found between height-for-age (H/A) (p=0.001) and weight-for-height (W/H) (p<0.001). The sensitivity of W/A for detecting malnutrition was 93%, H/A was 90%, MUAC was 86%, and TSF was 88%. The sensitivity was much lower for W/H and body mass index for age (55% for both). Conclusion: In our study, more than one-third of children with CLD were malnourished. Nutritional assessment using SGNA is a reliable method for evaluating nutritional status and is significantly correlated with common anthropometric measurements.

• Bulletin of the Korean Chemical Society
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• 제33권3호
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• pp.1042-1046
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• 2012

The kinetics and mechanism of the pyridinolysis ($XC_5H_4N$) of diethyl isothiocyanophosphate are investigated in acetonitrile at $55.0^{\circ}C$. The Hammett and Bronsted plots for substituent X variations in the nucleophiles exhibit the two discrete slopes with a break region between X = 3-Ac and 4-Ac. These are interpreted to indicate a mechanistic change at the break region from a concerted to a stepwise mechanism with rate-limiting expulsion of the isothiocyanate leaving group from a trigonal bipyramidal pentacoordinated intermediate. The relatively large ${\beta}_x$ values with more basic and less basic pyridines imply much greater fraction of frontside nucleophilic attack TSf than that of backside attack TSb.