• Title/Summary/Keyword: TS-1

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Growth Patterns of Temperature-sensitive Mutants of Bacillus Thuringiensis (Bacillus thuringiensis 의 Temperature-sensitive Mutants 분리와 특성 연구)

  • Lee, Hyung-Hoan;Lee, Hoon-Ku
    • Microbiology and Biotechnology Letters
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    • v.11 no.3
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    • pp.233-239
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    • 1983
  • Bacillus thuringiensis was mutagenized with UV light irradiation and nitrosoguanidine. Twenty-four tem perature-sensitive ts mutants were isolated at 42$^{\circ}C$ and classified into two groups by growth on nutrient agar at 42$^{\circ}C$. First is the lethal group, which did not grow at the nonpermissive temperature, the second is the reduced group whose growth was restricted from one-half to one-fourth, Thirteen ts mutants belong to the lethal group and eleven ts mutants belong to the reduced group. Auxotrophic mutant, A-N28 required five amino acids as growth factors, A-N65 also five amino acids, A-N92 seven, A-N115 four and A-N156 three. Bacillus thuringiensis wild type is resistant to penicillin, ampicillin, and cephalothin. The ts-Ul7l, A-N92 and A-Nl15 are sensitive to the three antibiotics. The ts -U601, -U603, -U604 and -Ul71 did not grow at the permissive temperature after temperature-shifting from 42$^{\circ}C$. Four auxotrophic mutants (A-N38, A-N65, A-N92 and A-Nl15) did not form spores in their cells.

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Development of an Input Impedance Evaluation of the AC-DC Transfer Standard for Low Level AC Voltage Standard (교류 저전압 표준용 교류-직류 변환기의 입력임피던스 평가기술 개발)

  • Kwon, Sung-Won;Jung, Jae-Kap;Lee, Sang-Hwa;Kim, Myung-Soo;Kim, Han-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.2
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    • pp.229-234
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    • 2008
  • An AC-DC transfer standard(TS) is used for the AC voltage standard in the range of 2 mV to 1000 V below 1 MHz. Micro-potentiometer(${\mu}Pot$) is used to evaluate the ac-dc transfer difference(ADD) of the TS below 200 mV range. The ADD of the TS were changed by the loading effect caused from the input impedance change of the TS depend on frequency. An input impedance evaluation technique of the TS using ${\mu}Pot$ has been developed.

Output Tracking Controller Design of Discrete-Time TS Fuzzy Systems (이산 시간 TS 퍼지 시스템의 출력 추종 제어기 설계)

  • 이호재;주영훈;박진배
    • Journal of the Korean Institute of Intelligent Systems
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    • v.11 no.1
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    • pp.45-51
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    • 2001
  • 본 논문은 이산 시간 Takagi-Sugeno (TS) 퍼지 시스템에 대한 출력 추종 제어기 설계에 관하여 연구한다. 출력 추종 제어기의 설계를 위하여 이산 시간 TS 퍼지 시스템을 불확실성을 포함한 선형 시스템의 집합으로서 표현하는 기법을 제시한다. 시스템의 상태에 대하여 어파인 변환을 하여 출력 추종 제어문제를 안정화 문제로 변환한다. 점근적 추종성능을 보장하는 제어기 설계를 위한 충분조건은 선형 행렬 부등식의 형태로 표현되면 주어진 제어기 설계의 해는 여러 가지 수치적 기법을 이용하여 효율적으로 구해질 수 있다. 트레일러 트럭의 임의의 위치에 후진 주차시키기 위한 제어기 설계의 예를 통하여 본 논문에서 제안한 추종 제어 기법의 효용성을 검증한다.

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Development of Hybrid 3D Broadcast platform Using MMT and MPEG2-TS (MMT와 MPEG2-TS 융합형 Hybrid 3D 방송 플랫폼 개발 Part 1 : 파일 생성 및 전송 파트)

  • Kang, Dong-jin;Kim, Da-young;Choi, Won-geun;Kim, Kyu-heon
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2015.07a
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    • pp.476-479
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    • 2015
  • 현 3D 실험방송은 좌 영상과 우 영상의 듀얼 스트림을 전송하는 서비스 호환 3D서비스를 제공하고 있다. 이와 같은 방식은 지상파를 이용해 전송되므로 제한된 대역폭에 따른 화질의 저하가 불가피한 것으로 여겨진다. 이에 본 논문에서는 상기 두 영상을 각각 기존의 지상파와 IP망으로 전송하여 화질의 열화 없이 안정적인 화질을 제공할 수 있는 MMT와 MPEG2-TS 융합형 Hybrid 3D 방송 시스템을 제안하였다. 제안한 시스템은 기존의 지상파를 통해 주영상을 MMT의 GFD mode를 이용하여 보조영상을 전송하여 두 영상의 동기화를 통해 고화질 스테레오스코픽 영상을 구성한다. 이를 위해 본 논문에서는 MPEG2-TS와 MMT의 GFD mode의 파일 구성을 각각 분석하고, 실험결과를 통해 제안한 MMT와 MPEG2-TS 융합형 Hybrid 3D 방송 시스템을 검증하였다.

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Theoretical Studies on the Base-Catalyzed Deprotonation of Sulfonyl-Activatec Carbon Acids

  • 김왕기;류완선;손창국;이본수;김창곤;이익준
    • Bulletin of the Korean Chemical Society
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    • v.18 no.2
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    • pp.208-212
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    • 1997
  • Theoretical AM1 MO studies are reported on the gas-phase deprotonations of N-methyl 4-((Y-phenylsulfonyl)pyridinium cations (Ⅳ) and N-(Y-benzyl) 4-((phenylsulfonyl)methyl pyridinium cations (Ⅴ) using NH3 as a base. Bronsted α values for deprotonation of Ⅳ and Ⅴ are 0.18 and 0.17, respectively. Bronsted β value of 0.53 is found for the deprotonation of Ⅳ and Ⅴ by substituted aniline bases. The negative transition state (TS) imbalances, Ⅰ(=α-β) < 0, are rationalized by the negative distance factor, Δd (=dp-dTS) < 0, where dp and dTS are the distance between anionic charge center and substituents in the product and in the TS, respectively. The inability of d-pπ-conjugation in Ⅳ, where instead n-σ* type sigma delocalization occurs, causes very little lag in the structural reorganization in the TS.

Effect of Microalgal Extracts of Tetraselmis suecica against UVB-Induced Photoaging in Human Skin Fibroblasts

  • Jo, Wol Soon;Yang, Kwang Mo;Park, Hee Sung;Kim, Gi Yong;Nam, Byung Hyouk;Jeong, Min Ho;Choi, Yoo Jin
    • Toxicological Research
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    • v.28 no.4
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    • pp.241-248
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    • 2012
  • Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVB-induced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.

Theoretical Studies on the Competitive Sn2 Reactions of O-Imidomethyl Derivatives of Phenols with OH-

  • Kim, Chang Gon;Jeong, Dong Su;Kim, Chan Gyeong;Lee, Bon Su;Jeong, Yeong Jin;Lee, Byeong Jun;Lee, Ik Jun
    • Bulletin of the Korean Chemical Society
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    • v.22 no.1
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    • pp.25-29
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    • 2001
  • Nucleophilic substitution reactions of O-imidomethyl derivatives of phenols with OH- were studied theoretically using the semiempirical AM1 and Solvation Model 2.1 (SM2.1) methods in the gas phase and aqueous solution, respectively. In the gas phase, the two reaction paths, in which the imide (1a) or phenol (1b) is functioning as a leaving group, can occur competitively. In contrast, in aqueous solution, path (1b) becomes more favorable than (1a) because the transition states (TS) of path (1b) are more stabilized by solvent. Differences in solvation energies are caused by the structural differences of TS, i.e., the TS via path (1b) is more dissociative than that via path (1a). Therefore we conclude that the solvent effects play an important role in the hydrolysis of O-imidomethyl derivatives of phenols. However, reactivity is dependent on the acidities of both the imide and the phenol fragments since the ρz values vary progressively from 4.2 (Z' = I) to 2.5 (Z' = IV) as the acidities of imide increase. These are in good agreement with the experimental results.

Ultrasonic Target Strength of Cyprinus Carpio in Accordance with Body Length and Body Weight (이스라엘 잉어 Cyprinus Carpio의 체장 , 체중별 초음파 표적강도에 관한 연구)

  • Shin, Hyoung-Ho;Yoon, Gab-Dong;Park, Hae-Hoon
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.25 no.4
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    • pp.191-199
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    • 1989
  • Research on the acoustic properties of fish has been carried out by a number of scientific workers from the earliest days of applying acoustic techniques to fish biomass estimates. This paper describes measurements of the target strength of Cyprinus Carpio, which measurements made at 50KHz in the experimental water tank. The results obtained are as follows: 1. The target strength(dB) of the fish has a directivity pattern quite similar to that of a transducer. The maximum value of target strength(dB) is obtained when the fish is insonified to its head-tail axis either from the dorsal or from the ventral side. 2. Empirical relationship between target strength(dB) and body length(cm) of the fish can be estimated as TS=20 Log L-65.4 where TS is the target strength of the fish and L is the body length of the fish. 3. The relationship between target strength(dB) and body weight(g) of the fish can be estimated as TS=6.7 Log W-53.7 where W is body weight of the fish.

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The relation between sewage sludge solubilization and extracellular polymeric substances (하수슬러지 가용화와 체외고분자물질(EPS)간의 관계)

  • Jeon, Byeong-Cheol;Nam, Se-Yong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.4
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    • pp.43-49
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    • 2019
  • In order to investigate the relation between sewage sludge solubilization and extracellular polymeric substances(EPS) during alkaline-ultrasonic pretreatment, SCOD/TCOD ratio, solubilization rate, VSS/TS ratio, VSS reduction rate, LB-EPS(Loosely-Bound EPS) and TB-EPS(Tightly-Bound EPS) were measured. At the condition of TS 1.0% and pH 12, solubilization rate increased by 27.7%, LB-EPS as Carbohydrate and Protein increased by 14.6 and 13.3 mg/L/g TS, respectively. Withal, VSS decreased by 26.7% and TB-EPS as Carbohydrate and Protein were extracted by 15.7 and 21.9 mg/L/g TS, respectively. Consequently, the concentrations of organic matter and LB-EPS increased and the trends appeared similarly. In addition, the concentrations trend of decreasing solid matter and extracted TB-EPS also appeared similarly.

Improvement of the efficiency from Computer-Generated Holograms by using TS algorithm and SA algorithm (TS 알고리듬과 SA 알고리듬을 이용한 컴퓨터 형성 홀로그램의 성능 향상)

  • Cho, Chang-Sub;Shin, Chang-Mok;Cho, Kyu-Bo;Kim, Soo-Joong;Kim, Cheol-Su
    • Korean Journal of Optics and Photonics
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    • v.16 no.1
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    • pp.43-49
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    • 2005
  • In this paper, we propose a method for optimizing a computer-generated hologram(CGH) by combining the Tabu Search(TS) algorithm with the Simulated Annealing(SA) algorithm. By replacing an initial random pattern of the SA algorithm with an approximately ideal hologram pattern of the TS algorithm, we design a CGH which has high diffraction efficiency(DE). We compared the performance of the proposed algorithm with the SA algorithm using computer simulation and an optical experiment. As a result, we confirmed diffraction efficiency and uniformity to be enhanced in the proposed algorithm.