• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.419-428
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• 2013

The objectives of this research were to determine ecological characteristics and chemical gradients in two different loach populations-Misgurnus anguillicaudatus ($M_a$) and Koreocobitis rotundicaudata ($K_r$). Eight chemical parameters were compared to test the chemical preferences between the two species. The population of Kr occurred more in better water quality, compared to the $M_a$-population. The $M_a$-population co-occurred more with tolerant species (TS) and omnivores, whereas the $K_r$-population co-occurred more with sensitive species (SS) and insectivores. The differences in the tolerance and trophic composition matched well with the result of stream health assessment, based on the multi-metric model of Index of Biological Integrity (IBI). The IBI value in habitat with the $M_a$-population was "19.6" (range: 8~40) indicating a fair condition, and the IBI values of $K_r$-population was 29.4 (range: 12~40) indicating good condition. Similar results were found in physical habitat health analysis (QHEI). Overall, data analysis showed that the $M_a$-population belong to the tolerant species and $K_r$-population belong to sensitive species in the tolerance classification.

• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.269-279
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• 2013

Low efficiency of somatic cell nuclear transfer (SCNT) is attributed to incomplete reprogramming of transfered nuclei into oocytes. Trichostatin A (TSA), histone deacetylase inhibitor and 5-aza-2'deoxycytidine (5-aza-dC), DNA methylation inhibitor has been used to enhance nuclear reprogramming following SCNT. However, it was not known molecular mechanism by which TSA and 5-aza-dC improve preimplantation embryo and fetal development following SCNT. The present study investigates embryo viability and gene expression of cloned porcine preimplantation embryos in the presence and absence of TSA and 5-aza-dC as compared to embryos produced by parthenogenetic activation. Our results indicated that TSA treatment significantly improved development. However 5-aza-dC did not improve development. Presence of TSA and 5-aza-dC significantly improved total cell number, and also decreased the apoptotic and autophagic index. Three apoptotic-related genes, Bak, Bcl-xL, and Caspase 3 (Casp3), and three autophagic-related genes, ATG6, ATG8, and lysosomal-associated membrane protein 2 (LAMP2), were measured by real time RT-PCR. TSA and 5-aza-dC treatment resulted in high expression of anti-apoptotic gene Bcl-xL and low pro-apoptotic gene Bak expression compared to untreated NT embryos or parthenotes. Furthermore, LC3 protein expression was lower in NT-TSA and NT-5-aza-dC embryos than those of NT and parthenotes. In addition, TSA and 5-aza-dC treated embryos displayed a global acetylated histone H3 at lysine 9 and methylated DNA H3 at lysine 9 profile similar to the parthenogenetic blastocysts. Finally, we determined that several DNA methyltransferase genes Dnmt1, Dnmt3a and Dnmt3b. NT blastocysts showed higher levels Dnmt1 than those of the TSA and 5-aza-dC blastocysts. Dnmt3a is lower in 5-aza-dC than NT, NTTSA and parthenotes. However, Dnmt3b is higher in 5-aza-dC than NT and NTTSA. These results suggest that TSA and 5-aza-dC positively regulates nuclear reprogramming which result in modulation of apoptosis and autophagy related gene expression and then reduce apoptosis and autophagy. In addition, TSA and 5-aza-dC affects the acetylated and methylated status of the H3K9.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1118-1125
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• 2014

The past IMF foreign exchange crisis and subprime financial crisis had a big influence on variability of macroeconomics, even if the origin of its occurrence might be different. This not only had a significant infrequence on the overall industries, but also produced many insolvent companies by being closely linked with a management environment of an individual construction company leading the construction industry. The purpose of this research is to investigate characteristics of management condition of construction company according to the size of construction company using KMV model developed on the basis of the Black & Scholes option pricing theory. This research has set 28 construction companies listed to KOSPI/KOSDAQ for applying the KMV model and measuring the level of the default risk of construction companies. The data was retrieved from TS2000 established by Korea Listed Companies Association (KLCA), Statistics Korea. The analysis period is between first quarter of 2004 and fourth quarter of 2010. This research examine characteristics of the level and fluctuation process of the management condition of construction company according to the size of construction company.

• International Journal of Highway Engineering
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• v.12 no.4
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• pp.131-137
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• 2010

The stormwater runoff from rest areas in highways are known as more polluted compared to highways because of more vehicle activities. This study is performed to find pollutant characteristics in the rest areas in the magnitude of statistical pollutant concentrations during storms. Washoff characteristics of pollutants from rest areas by monitoring of rainfall, runoff rate and runoff samples were evaluated. High concentrations of pollutants in runoff were observed at the beginning of runoff and rapid decrease thereafter, indicating that first-flush effects are clearly occurred. Event Mean Concentrations(EMCs) of TSS, COD, TN, and TP are estimated to be in the range of 31.04-127.11mg/L, 35.5-369.5mg/L, 2.62-9.86 mg/L, and 0.53-1.96mg/L, respectively. Heavy metals in runoff showed relatively high values, possibly due to the abrasion of brake pad or tire while cars are slowly moving for parking. EMCs of total Pb, total Cu, and total Ni are in the range of $1206-16293{\mu}g/L$, $237-7906{\mu}g/L$, and $53-6372{\mu}g/L$, respectively. Pollutant loading per rest area calculated by using EMC, flowrate and target area is also described for each pollutant.

• International Journal of Highway Engineering
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• v.9 no.4
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• pp.185-192
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• 2007

Newly constructed road is a requisite to be able to carry out BMPs (Best Management Practices) under TMDL(Total Maximum Daily Load) program of the Ministry of Environment. BMPs require pollutant source control during road construction and wash off reduction plan as well as maintenance practices subsequent to construction on the purpose of discharging the minimum wash off non-point source pollutants. The objective of this study is to provide supportive discharged data in evaluating the discharged non-point pollutant load from a highway toll gate area. It can be applied to manage non-point source pollutants on roads. The results validate the first flush phenomenon that it is known to be one of the wash off characteristics in paved area. In addition, the load per unit area and load per unit rainfall duration applying EMC are calculated. The mean load per unit rainfall duration is assessed to be $533.7mg/m^2-hr$ for TSS, $396.2mg/m^2-hr$ for COD, $17.0mg/m^2-hr$ for TN, and $4.8mg/m^2-hr$ for TP. These results show the unitload taken from monitoring are higher than the unit load suggested in the TMDL. It is important to adopt real pollutant unit for road to be able to perform BMP successfully.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.435-435
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• 2012

농경지 중에서 밭은 논보다 농약 비료 사용률이 매우 높기 때문에 논에 비해 비점오염물질 배출량이 훨씬 심각한 것으로 조사되고 있어 밭경지 비점오염저감기법 개발 및 체계적인 제어대책 수립이 무엇보다도 절실하다. 현재 선진국을 중심으로 다양하게 제시되고 있는 밭경지 비점오염 제어기법들 중 초생대에 대한 성공적 연구사례 및 적용사례에 대한 자료가 상당히 많이 제시되고 있으나, 우리나라의 경우 초생대를 적용한 밭경지 비점오염저감에 대한 기술개발 및 체계적인 연구가 아직 미비한 실정이다. 따라서 본 연구는 초생대의 현장설치 및 모니터링을 통해 초생대의 비점오염부하 저감효과에 대한 기초조사를 실시하고 우리나라 밭경지에서의 적용가능성을 알아보고자 한다. 초생대 현장 실험을 위한 시험포는 경상북도 군위군 효령면에 위치한 경북대 농생대 부속농장 밭경지 $1,500m^2$ (455평)를 선정하였다. 시험포는 1개의 대조구와 6개의 처리구로 구성하였으며, 각 시험구의 크기는 길이 12m ${\times}$ 폭 4m (초생대 길이 2m ${\times}$ 폭 4m 포함)로 하였다. 초생대 조성을 위한 초종선정은 초생대 조성이 용이하고 관리효율성이 높은 초종으로서 손쉽게 구할 수 있고, 우리나라 기후와 토양특성에 적합하며 초생대 기능에 부합한 것으로 선정하였다. 초생대 시험포장에 재배할 작물은 우리나라 대표 밭작물인 콩으로 선정하였으며, 작물재배를 위한 퇴비, 비료, 제초제 등은 농촌진흥청에서 제시한 표준재배법에 준하여 시용하였다. 초생대 비점오염저감효과를 평가하기 위해서 시험포장에 플륨, 수위계, 강우계 등으로 구성된 모니터링시스템을 설치하였으며, 플륨의 수위-유량 캘리브레이션을 실시하였다. 실험을 위해 2회의 인공강우와 1회의 자연강우에 대한 모니터링을 실시하였다. 그 결과 초생대 조성이 유출률에 상당한 영향을 미치는 것으로 조사되었다. 인공강우의 경우 초생대 설치에 따라 유출률의 범위가 14.5~95.8% 정도로 감소되는 현상을 보였으며, 자연강우에서도 6.1~11.3% 정도의 유출률 감소를 보였다. 초생대 시험구별 유출률에 차이를 나타낸 이유는 현장실험시의 시험구별 지면조건과 초생대 초종별 특성(초장, 경경, 밀도 등)의 차이에 기인한 결과로 사료되었다. 비점오염저감효과 측면에서는 기존 밭농사 방식을 그대로 채택한 대조구에 비해 초생대 시험구에서 TS의 경우 15.6~90.3%, T-P의 경우 49.9~87.8%, T-N의 경우 6.7~91.1%의 저감율을 각각 나타내었다. 이러한 결과들을 통해 초생대 기법이 우리나라 밭경지 비점오염부하를 저감시키는데 유용하게 활용될 수 있을 것으로 판단된다.

• Journal of Wetlands Research
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• v.20 no.1
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• pp.72-79
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• 2018

Two planter boxes were monitored during their initial year of operation to be able to assess their stormwater runoff and pollutant reduction capabilities and investigate on the design factors affecting their performance. One of the planter boxes provided 85-100% runoff volume reduction for rainfall less than 15 mm and rainfall intensities lower than 5 mm/hr. This reduced to 50-64% during higher rainfall intensities and depths of up to 50 mm. Suspended solids, organics, nutrients, and heavy metals were satisfactorily removed at a range of 40-95%. The other planter box, however, did not produce outflow in all the events and allowed total capture of stormwater. The uncertainty regarding the fate of the runoff in that case required an investigation of the planter box's actual drainage and underground conditions which was deemed outside the scope of the study. Nonetheless, several design improvements and retrofits were suggested based on the provisions of current design guidelines to ensure that the hydraulic and water quality goals are achieved without potential damage to nearby structures. Moreover, continuous monitoring data is required to provide more accurate design evaluation and can serve as a guide in the construction of similar facilities in the future.

• Korean Journal of Veterinary Service
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• v.20 no.2
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• pp.143-150
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• 1997

Total viable cells and lactose non-fermenting cells were counted from animal feedstuffs (n=65). And isolation of Gram negative lactose nonfermenting enterobacteria and antibiotics susceptibility of isolates were performed. 1. The ranges of total viable cells / lactose non-fermenters in animal feedstuffs from Korean cattle were counted as 9$\times$$10^4$-1$\times$$10^7$ / 1$\times$$10^2$-6$\times$$10^3$, milking cow as 1$\times$$10^4$-2$\times$$10^8$ / 2$\times$$10^2$-8$\times$$10^3$, pig as 1$\times$$10^4$-1$\times$$10^6$ / 2$\times$$10^2$-6$\times$$10^3$, and chicken as 7$\times$$10^4$-1$\times$$10^9$ / 4$\times$$10^2$-1$\times$$10^5$ cfu/g, respectively. 2. Among the 214 isolates from feedstufs, 87 from Chinan(n=23), 66 from Changsu (n=23) and 61 from Mooju(n=19) were isolated. Of these isolates, 60 from pigs (n: 19), 51 from milking cows(n=15), 45 from chikens(n=11) and 58 from Korean cattle(20) were isolated. 3. Among the 6 genuses of Gram negative lactose nonfermenting enterobacili, Salmonella sp, Y pseudotuberculosis, Ent agglomerans and Sal choleraesuis were frequently encountered. 4. A majority of isolates were sensitive to 19 antibiotics, singly or in combination. These isolates were completely susceptible to Cp, Gm, Imp and Pi, 93% to Ak and To, 73% to Cax and Ts, 66% to Cft and Tim, 46-53% to Caz, Cf and Cz, 33-40% to Am, Azt, Cfz and Ti, and 6% to Cfx, in order, but not susceptible to Crm. 5. Among the antibiotic resistant strains, a total of 23 resistant patterns was noted, and of these Crm 40(18.7%), Am Cf Cfx Cfz Crm Ti 27(12.6%), each of Azt Ctx Crm and Azt Cax Caz Cft Cfx Crm 22(10.3% ) were frequently encountered.

• Korean Journal of Weed Science
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• v.18 no.2
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• pp.136-145
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• 1998

This study was conducted to determine the growth characteristics, and the effect of plant growth regulators on the sprouting and growth of purple nutsedge(Cyperus rotundus L.) in order to establish effective control system in lawn ground. The flowering of purple nutsedge was initiated 30 days after transplanting regardless of the transplanting time. Low temperature less than $10^{\circ}C$ after flowering was required for tuber formation, showing that the tuberization was related to air temperature. Shoot number and dry weight of underground portion of purple nutsedge was slightly affected by plant growth regulators such as benzylamino purine, abscisic acid, brassinosteroid and jasmonate. Imazaquin applied at 1, 2 and 3 weeks after transplanting induced multi-shooting and inhibited shoot growth indicating that the herbicide played a role as plant growth regulator at a concentration of 30 and 60g ai/10a. The greatest inhibition of purple nutsedge was obtained by pyrazosulfuron-ethyl as applied 1 weeks after transplanting, showing almost 100% control of purple nutsedge. Tuber of purple nutsedge composed of 61.83% of moisture, 31.60% of carbohydrates, 4.03% of crude protein, 1.57% of crude fat and 0.97% of crude ash.

• Korean Journal of Materials Research
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• v.9 no.12
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• pp.1252-1262
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• 1999

The effect of microstructures on the strength-flangeability of Nb bearing hot-rolled high strength steel was investigated in order to improve the strength-flangeability of conventional TS 580MPa grades HSLA steel for the automotive wheel disc. The low temperature coiling method using 3-step controlled cooling pattern after hot rolling was effective to produce the Nb-bearing high strength steel with the polygonal ferrite and bainite duplex microstructures. It was suggested that the suppressed precipitation of grain boundary cementites and the decreased hardness difference between ferrite matrix and bainite cause the excellent stretch-flangeability of ferrite-bainite duplex microstructure steel. Therefore, the formation and propagation of microcracks were suppressed relative to the conventional HSLA steel with ferrite and pearlite microstructure. In addition, the elongation was improved as compared with that of hot-rolled steel sheets using conventional early cooling pattern because the volume fraction of polygonal ferrite was increased.