• Title/Summary/Keyword: TRA

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Hierarchical Routing Protocol for Traffic-Balanced DiffServ Network Architecture (DiffServ망 구조에서 트래픽 분산을 위한 계층적 라우팅 프로토콜)

  • In, Chi Hyeong
    • The Magazine of the IEIE
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    • v.30 no.5
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    • pp.95-95
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    • 2003
  • 현재의 라우팅 프로토콜은 다양한 사용자 요구를 만족시켜주기 위해서는 네트워크의 처리량을 최대화하고 동시에 사용자의 요구 시 QoS를 보장해주는 기법이 요구되고 있다. 기존의 최단경로 라우팅 프로토콜은 단일경로 라우팅으로 인해 병목현상의 단점을 지니고 있다. 즉, 원천과 목적지간 최단경로는 낮은 활용도를 나타내는 경로들이 많이 존재하지만 단일경로를 선택하므로서 폭주(congestion)의 발생확률이 높다. 최근에 들어 사용자의 QoS 요구 시, 다양한 QoS를 패킷 네트워크에서 처리할 수 있도록 IETF에서 DiffServ, RSVP, MPLS 등과 같은 패킷 QoS 기법에 대한 표준화 작업이 진행중이며, 그 중에서 Diffserv 네트워크가 대표적이다. 따라서 본 논문에서는 이 DiffServ 네트워크상에서 다양하게 유입되는 트래픽의 종류에 따라 사용자의 응용에 적절히 대응하여 트래픽을 처리하는 라우팅 기법 및 알고리즘을 연구하고 기존의 최선형 (Best effort) 트래픽을 처리하기 위한 트래픽 분산 라우팅 프로토콜 (Traffic-Balanced Rout-ing Protocol''TBRP)을 제안하였으며, 최적의 중간 노드를 선택하여 높은 순위의 상호형 데이터를 처리하기 위한 계층적 라우팅 프로토콜(또ierarchicalTra(fic-Scheduling Routing Protocol : HTSRP)을 연구하였다. 본 연구에서 제시한 프로토콜은 유, 무선망의 통합에 따른 다양한 엑세스망과 백본망에 유연한 트래픽 처리기법으로서 계층적 라우팅 알고리즘으로 적합하였다. 본 실험에서는 사용자의 QoS요청 시 제공되는 상호형 또는 스트리 밍 데이터를 위한 HTSRP_Q(Hierarchical Traffic-Scheduling Routing Pro-tocol for QoS)에 대해 성능이 우수함을 입증하였으며, 각 엑세스 단에서 요청하는 QoS 파라미터에 따라 자원을 최적화하여 QoS를 보장하고, 특히 지연에 민감한 트래픽을 처리하였으며, 제안한 프로토콜을 이용하여 사용자 요구 트래픽 종류에 따라 대화형 클래스, 스트리밍 클래스, 높은 순위의 상호형 클래스, 낮은 순위의 상호형 클래스, 그리고 background 클래스등 5개의 서비스 클래스로 분리하여 트래픽 특성에 맞게 처리할 수 있었다. QoS 관련 실험에서는 QoS 요청데이터를 균등하게 1에서 10Mbps 사이에 분포하였고 연결된 호에 대한 지속시간은 5분으로 하였다. 이러한 환경에서 프로토콜을 MaRS에 의해 실험을 하였고 기존의 거리-벡터 라우팅과 링크-상태 라우팅 프로토콜과 비교해서 처리량, 메시지 손실, 블럭킹율 등에서 비교적 우위의 성능을 확인할 수 있었으며, 특히, 차별화된 서비스의 특성에 맞게 라우팅 기법을 적용하므로서 망의 효율성과 안정성을 꾀할 수가 있었다. 연결 수 대 처리량에서는 HTSRP 프로토콜이 연결이 적을 때 DVR, LSR보다 우월하였으며 특히, 선형을 유지하였다. 연결 수 대 패킷 손실에서 HTSRP프로토콜에서 메시지 손실은 연결의 수가 낮거나 높을 때 다른 DVR과 LSR 라우팅 프로토콜과 유사한 결과를 나타내었다. Hotspo에서 TBRP, HTSRP프로토콜은 hotspot 연결의 수가 9일 때까지 DVR, LSR 보다 좋은 처리량를 나타냈고 HTSRP는 연결의 수가 6 이상일 때 가장 높은 처리량을 나타내었다. 일반 트래픽과 QoS 트래픽이 흔재할 경우는 트래픽이 증가할수록 HTSRP_Q가 가장 월등하였으며 , 로드가 증가할수록 낮은 블록킹률을 나타내었다. 본 논문에서는 점대점 전송을 기반으로 하였다. 앞으로 다양한 응용 S/W는 멀티캐스트 기반이 예상되므로 멀티캐스트 라우팅에 대한 연구가 필요하다. 본 논문의 프로토콜은 원천과 목적지간의 최단경로가 폭주상태가 아닌 해당 중간 노드를 이용한다. 최단경로의 모든 링크상의 트래픽 부하가 낮을 때 중간노드의 사용은 지연을 증가시킨다. 향후 최적의 성능을 위해 보완이 필요하다. 아울러, 2계위에서는 일반 트래픽과 QoS 트래픽이 혼재할 때 자동으로 네트워크의 효율적을 고려한 방법 선택이 필요하다.

Hierarchical Routing Protocol for Traffic-Balanced DiffServ Network Architecture (DiffServ망 구조에서 트래픽 분산을 위한 계층적 라우팅 프로토콜)

  • 인치형
    • The Magazine of the IEIE
    • /
    • v.30 no.5
    • /
    • pp.551-551
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    • 2003
  • 현재의 라우팅 프로토콜은 다양한 사용자 요구를 만족시켜주기 위해서는 네트워크의 처리량을 최대화하고 동시에 사용자의 요구 시 QoS를 보장해주는 기법이 요구되고 있다. 기존의 최단경로 라우팅 프로토콜은 단일경로 라우팅으로 인해 병목현상의 단점을 지니고 있다. 즉, 원천과 목적지간 최단경로는 낮은 활용도를 나타내는 경로들이 많이 존재하지만 단일경로를 선택하므로서 폭주(congestion)의 발생확률이 높다. 최근에 들어 사용자의 QoS 요구 시, 다양한 QoS를 패킷 네트워크에서 처리할 수 있도록 IETF에서 DiffServ, RSVP, MPLS 등과 같은 패킷 QoS 기법에 대한 표준화 작업이 진행중이며, 그 중에서 Diffserv 네트워크가 대표적이다. 따라서 본 논문에서는 이 DiffServ 네트워크상에서 다양하게 유입되는 트래픽의 종류에 따라 사용자의 응용에 적절히 대응하여 트래픽을 처리하는 라우팅 기법 및 알고리즘을 연구하고 기존의 최선형 (Best effort) 트래픽을 처리하기 위한 트래픽 분산 라우팅 프로토콜 (Traffic-Balanced Rout-ing Protocol'TBRP)을 제안하였으며, 최적의 중간 노드를 선택하여 높은 순위의 상호형 데이터를 처리하기 위한 계층적 라우팅 프로토콜(또ierarchicalTra(fic-Scheduling Routing Protocol : HTSRP)을 연구하였다. 본 연구에서 제시한 프로토콜은 유, 무선망의 통합에 따른 다양한 엑세스망과 백본망에 유연한 트래픽 처리기법으로서 계층적 라우팅 알고리즘으로 적합하였다. 본 실험에서는 사용자의 QoS요청 시 제공되는 상호형 또는 스트리 밍 데이터를 위한 HTSRP_Q(Hierarchical Traffic-Scheduling Routing Pro-tocol for QoS)에 대해 성능이 우수함을 입증하였으며, 각 엑세스 단에서 요청하는 QoS 파라미터에 따라 자원을 최적화하여 QoS를 보장하고, 특히 지연에 민감한 트래픽을 처리하였으며, 제안한 프로토콜을 이용하여 사용자 요구 트래픽 종류에 따라 대화형 클래스, 스트리밍 클래스, 높은 순위의 상호형 클래스, 낮은 순위의 상호형 클래스, 그리고 background 클래스등 5개의 서비스 클래스로 분리하여 트래픽 특성에 맞게 처리할 수 있었다. QoS 관련 실험에서는 QoS 요청데이터를 균등하게 1에서 10Mbps 사이에 분포하였고 연결된 호에 대한 지속시간은 5분으로 하였다. 이러한 환경에서 프로토콜을 MaRS에 의해 실험을 하였고 기존의 거리-벡터 라우팅과 링크-상태 라우팅 프로토콜과 비교해서 처리량, 메시지 손실, 블럭킹율 등에서 비교적 우위의 성능을 확인할 수 있었으며, 특히, 차별화된 서비스의 특성에 맞게 라우팅 기법을 적용하므로서 망의 효율성과 안정성을 꾀할 수가 있었다. 연결 수 대 처리량에서는 HTSRP 프로토콜이 연결이 적을 때 DVR, LSR보다 우월하였으며 특히, 선형을 유지하였다. 연결 수 대 패킷 손실에서 HTSRP프로토콜에서 메시지 손실은 연결의 수가 낮거나 높을 때 다른 DVR과 LSR 라우팅 프로토콜과 유사한 결과를 나타내었다. Hotspo에서 TBRP, HTSRP프로토콜은 hotspot 연결의 수가 9일 때까지 DVR, LSR 보다 좋은 처리량를 나타냈고 HTSRP는 연결의 수가 6 이상일 때 가장 높은 처리량을 나타내었다. 일반 트래픽과 QoS 트래픽이 흔재할 경우는 트래픽이 증가할수록 HTSRP_Q가 가장 월등하였으며 , 로드가 증가할수록 낮은 블록킹률을 나타내었다. 본 논문에서는 점대점 전송을 기반으로 하였다. 앞으로 다양한 응용 S/W는 멀티캐스트 기반이 예상되므로 멀티캐스트 라우팅에 대한 연구가 필요하다. 본 논문의 프로토콜은 원천과 목적지간의 최단경로가 폭주상태가 아닌 해당 중간 노드를 이용한다. 최단경로의 모든 링크상의 트래픽 부하가 낮을 때 중간노드의 사용은 지연을 증가시킨다. 향후 최적의 성능을 위해 보완이 필요하다. 아울러, 2계위에서는 일반 트래픽과 QoS 트래픽이 혼재할 때 자동으로 네트워크의 효율적을 고려한 방법 선택이 필요하다.

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A Case of Acute Respiratory Distress Syndrome Induced by All-Trans-Retinoic Acid (ATRA로 유발된 급성호흡곤란증후군 1예)

  • Kim, Cheol;Ko, Won-Ki;Kwon, Seung-Hyun;Kang, Shin-Myung;Kim, Chang-Nyun;Yang, Dong-Gyoo;Kim, Se-Kyu;Chang, Joon;Kim, Sung-Kyu;Lee, Won-Young;Yang, Woo-Ik
    • Tuberculosis and Respiratory Diseases
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    • v.49 no.1
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    • pp.93-98
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    • 2000
  • Acute respiratory distress syndrome (ARDS) has been reported to be associated with a variety of medical and surgical conditions, including All-trans-retinoic acid (ATTA). ATRA is very efficaceous drug to acute promyelocytic leukemia (APL). This drug can induce complete remission at APL without fatal risk of disseminated intravascular coagulation. But ATRA treatment, sometimes, produces the symptoms of fever, weight gain and acute respiratory distress, renal function impairment. The causes of these symptoms are not fully proved, but supposed as the result of leukostasis and capillary leak syndrome from excessive leukocyte differentiation and cytokines release. Recently, we experienced a 24-year-old woman who complained gum bleeding for 6 days. At bone marrow biopsy, she was diagnosed as APL. 2 days after ATRA treatment, she was suffered from the symptoms of dyspnea and general ache. At laboratory examination, total leukocyte count was 50,400/$mm^3$, $PaO_2$ was 42.5 mm Hg and chest PA revealed the findings compatible with ARDS. Treatment with low dose ara-C, corticosteroid and general supportive cares were tried. Within 3 days after treatment, the patient recovered from ARDS by evidence of arterial blood gas study and chest radiographs. She has acquired complete remission of APL with maintenance of A TRA. And so, we present this case with a review of related literatures.

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Effect of Additive on the Chemical Composition of Tra Catfish (Pangasius hypophthalmus) By-product Silages and Their Nutritive Value for Pigs

  • Thuy, Nguyen Thi;Lindberg, Jan Erik;Ogle, Brian
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.6
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    • pp.762-771
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    • 2010
  • Two experiments were conducted to determine i) the fermentation characteristics of catfish by-product (C) ensiled with rice bran (RB) or sugarcane molasses (M) in different ratios, and ii) the digestibility of the silages in growing pigs. In the ensiling experiment, there were three ratios of C, ensiled with RB or M, of 8:2, 7:3 and 6:4 (wet basis for C and air-dry basis for RB and M). The six treatments were CRB8:2, CRB7:3, CRB6:4, CM8:2, CM7:3 and CM6:4, with 3 replications per treatment and seven sampling times. The pH of CRB7:3 and CRB6:4 decreased (p<0.05) from the first week and stayed stable until 8 weeks of ensiling, but the pH did not decrease in CRB8:2. The pH of CM8:2, CM7:3 and CM6:4 decreased rapidly from the first week until week 8. Dry matter (DM) and crude protein (CP) contents were slightly lower when catfish by-product was ensiled with RB than with M. Ammonia content in all treatments increased (p<0.05) during ensiling. Lactic acid content in silages with molasses increased from the first week, with the highest value at week 4. However, the lactic acid content in CRB8:2 did not increase with time and had the lowest value of all treatments. Acetic acid proportions of total volatile fatty acids were low at day 0, with the highest value at day 7, decreasing slowly until 8 weeks. The butyric acid proportion was highest at day 0 and decreased up to week 8. The proportion of propionic acid increased during ensiling. The digestibility experiment had a 4${\times}$4 Latin-square design and included 4 castrated crossbreed (Yorkshire${\times}$Landrace) male pigs fed four diets. The basal diet (BD) included rice bran, broken rice and maize meal, and the other three diets included fish meal (FMD) or catfish by-product ensiled with rice bran (CRBD) or sugarcane molasses (CMD). The silages chosen were those which gave the best results in the ensiling experiment for each additive. The coefficient of total tract apparent digestibility (CTTAD) of DM and organic matter (OM) was not significantly different among treatments. The CTTAD of CP was not different among the silage diets, but was lower in BD (p<0.01). The CTTAD of ether extract (EE) was higher in the catfish by-product silage diets than in FMD and BD. There was no significant difference in the digestibility of DM, OM, CP and EE between the fish meal and the by-product silages. In conclusion, catfish by-product can be successfully preserved by ensiling. Moreover, the total tract apparent digestibility of OM, CP and EE in ensiled catfish by-product was comparable with that of fish meal.

A comparative study of the Dunhuang Costume on the Folk Song's words in Dnag's period of the China. (중국.고대 돈황가중의 복식 연구)

  • 김은주
    • Journal of the Korean Society of Costume
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    • v.22
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    • pp.159-176
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    • 1994
  • This study's purpose is to find the ancient costume of the folk song's words on Dunhuang manuscripts in the background wass-carvings sculptures and several art works. Dunhuang had played the part of major route between ancient China and Europe. It is located on kam-suksong the west of China. 1. In the aspect of costume arrangement hair Styling is represented by Naegagyo, Wangyesa, Kyongburak, Bonkwyun, Pogurak, Chonsonja, Namgaja, Tongson-ga, Ojesa, Chanboja in Dunhuang manuscripts. tugue is sim-ilar to Sabyokkwanja in Naegagyo as of that time these kinds of Kwan-ja was very popular. It was told that emperor's wife concubine and maid of honor would wear kwnaja favorably. And the kinds of Tugeu is PuyongKwyunkye, Aangunkye, Chunkye from Kyuwanjon except Sangunkye, Ounkye in Dunhuangsa. 2. Jangdaegy is guoted from Kyuw-anjon -A series of the chinese history book-tells us about women's make-up . The narmes of make-up are Hongjang Unjan--g, Yiljang chwejang, Tam-ajang Murgjang, Sanagjang, Light-make up are referred in Naegagyo is included as packjang Besides we can find other make-up skills Huphwaro and Rujang from the Kaewonch onbon-wisa which was Yangkwibi's episodes. We can find the a corpus of song's words about cosmetic custom in Kor-ea. Which is 'Haecho' folk song in the souther-en costal regions 'Jinggum' folk song in Kim-hae regions and so on.... It's words is similar to Dunhuangsa in China. 3. Bichun inherited to China with India Bud-dism art in the middle of Dunhuang wall-pain-tings is one of the important Buddist saint. Sometimes it is painted with Giakchun Its arms of fly-dnacing in heaven with colored belt on body has almost the form of play on the musical instrument, We can see seasily the form in the painting of Buddist ser-mon. It was progressed and changed with the background of chinese traditional culture. When Dang's period came the color was chan-ged lighting dark blue has rhythmical and viv-id power of life. 4. Among the corpus of song's words which express the singers' system and rites of their tradition or habits. Dunhuang manuscripts des-cribes on behalf of the folk song's words in tra-ditional costume. This is following: (1) Naegagyo o sabyokwanja o Chyrasam o Rashang (2) Wangyesa o Sokryugun o Costume for fisher man (3) Bungajang of the chanboja o Ajang (4) Pongkwyun o Chongui (5) Tongsongs o Junofore (6) Namgaja o thin-Rasam (7) Yuchungnang o Hongsam o Sokryugun : The color of red costume (8) Kyongburak o Hongrasam o Sokryugun : Costume for charming woman Therefore traditional costume could be extracted according to the Dunhuang folk song's words out of culture such as learning rit-es three-obedience four-virtues and in-struction. As the same time it reflects ancient China's characteristics directly. I would endeavor to development for the history of the costume furher creative design and participat-ed in research activity with concerns forward continuously.

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A study on the Artistic Formativeness Represented in the Materials of the Modern Fashion Design (현대패션 디자인 소재에 표현된 예술적 조형성에 관한 연구)

  • 이효진
    • Journal of the Korean Society of Costume
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    • v.32
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    • pp.163-182
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    • 1997
  • The purpose of this study was intended to analyze artistic formativeness represented in the modern fashion design materials. Nowadays the modern fashion design materials is characterized by variety. The fashion materials the most important of fashion is called a softwear in textile industry and emerged as a new field. Fashion design also escaped from the tra-ditional restraint and extended to pursue lib-eral clothes and the designer's creative inten-tion has resulted in conceptual and conven-tional alteration as a new mode of plastic ex-preseeion. As a results of analysis of the formative fea-ture of Art style represented in the modern fashion design materials. First Surrealism had been searching for a new way of confronting a social and rtistic environment that was stifling and repugnant to them. Accordingly the wide applicaytion of the various object due to the thought of Sur-realism through the modern fashion materials brought the diversification and individua-lization of the modern fashion design. Second Pop Art that is made modern mech-anic culture and commercialism brought the great transformation in the history of Art out of the existing style and the sense of ex-pression. And the plastic characteristics of Pop Art that has a gravity as modern fashion materials on a them of the elements of popular consumer's society. That is the common and cheap objects were introduced into fashion materials itself or pat-tern. Third Minimalism is a trend in art att-empting to seek essence of the object by presenting simple and disciplined expressions by minimal formative means. The features of external form are simplicity clarity unrelated composition and symmetrical structure. Mini-malism was a quest for basic elements repre-senting the fundamental esthetic values of art. The minimal expression in modern fashion materials mean fabrics with simple surface and is contained using simple geometric pattern or utilizing textiles without any patterns. Fourth Eroticism is a kind of psychological revolution in the development of human civilization and is deeply rooted in the cultural tradition of myth religion customs and art. So Eroticism must be distinguished from a mere animal reproduction. These erotic char-acteristics were holded the meanings of sexu-ality through the modern fashion materials. Especially it was expressed the sexual part of body was nakedly showed through trans-parent materials or semitransparent like metalic and opaque materials. Recently the various kinds of new materials such as paper vinyle plastic metal as well as human body were applied to new fashion de-sign. First of all the new materials will give modern fashion designers stimuli and inspi-ration. It can also express values of moderners to despise materialism and uniformed modern society and to recover humanity and self-actualization.

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Effect of retinoic acid on the radiosensitivity of normal human oral keratinocyte (Retinoic acid가 사람 정상 구강각화세포의 방사선감수성에 미치는 영향에 관한 연구)

  • Lee Jean;Heo Min-Suk;Lee Sam-Sun;Oh Sung-Ook;Lee Sul-Mi;Choi Hang-Moon;Choi Soon-Chul;Park Tae-Won
    • Imaging Science in Dentistry
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    • v.33 no.2
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    • pp.97-105
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    • 2003
  • Purpose : To evaluate the effect of all-trans-retinoic acid (ATRA) on the radiosensitivity of normal human oral keratinocyte (NHOK). Materials and methods: Relative cell survival fraction including SF2 (survival fraction at 2 Gy) was calculated on the basis of colony formation assay. Data were fitted to the linear-quadratic model to establish the survival curve and calculate α and β values. Using flow cytometry at 1, 2, 3, 4, and 5 days after exposure to 2 and 10 Gy irradiation, cell cycle arrest and apoptosis were analysed. To understand the molecular mechanism of the radiosensitization of ATRA on NHOK, proteins related with apoptosis and cell cycle arrest were investigated by Western blot analysis. Results: Treatment with ATRA resulted in a significant decrease of SF2 value for NHOK from 0.63 to 0.27, and increased α and β value, indicating that ATRA increased radiosensitivity of NHOK. ATRA increased LDH significantly, but increasing irradiation dose decreased LDH, suggesting that the radiosensitizing effect of ATRA is not directly related with increasing cell necrosis by ATRA. ATRA did not induce appotosis but increased G2 arrest after 10 Gy irradiation, implying that the increased radiosensitivity of NHOK may be due to a decrease in mitosis casued by increasing G2 arrest. ATRA inhibited the reduction of p53 at 3 days after l0Gy irradiation and increased p21 at 1 day after 10 Gy irradiation. Further study is required to determine the precise relationship between this effect and the radiosensitizing effect of A TRA. Conclusion: These results suggested that ATRA increase radiosensitivity by inhibiting mitosis caused by increasing G2 arrest.

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A Study on the Factors of Individual Acceptance for Online Game (온라인게임의 개인적 수용 요인에 관한 연구)

  • Yoon, Gun-Woo;Kim, Won-Joon;Ryu, Seoung-Ho
    • Journal of Korea Game Society
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    • v.9 no.3
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    • pp.107-119
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    • 2009
  • Online games belong to a technology-oriented service industry that has emerged from the assimilation of game contents and communication networks in the respective realms of culture technology (CT) and information technology (IT). This study examines the individual acceptance of online games as entertainment-oriented technology based on the belief-attitude-intention paradigm and the underlying hedonic and utilitarian motivations. To measure the latent variables influencing the acceptance process, a structured questionnaire was developed based on the existing Technology Acceptance Model. Research model analysis and hypothesis testing were carried out using a structural equation model. Results indicated that latent variables reflecting hedonic and utilitarian characteristics had a significant influence on user behavior in the acceptance of online games. By uncovering the core factors in! fluencing the acceptance of online games and thereby theoretically verifying the latent variables influencing the acceptance of entertainment-oriented technology, this study provides strategic implications for business models by game companies aimed at attracting a large user base and dominating the market.

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Consideration on Scriptural Foundation Viewpoint of Seokgatap - Centering on Implication of "Gyeonbotappum" - (석가탑(釋迦塔)의 경전적인 건립시점 고찰 - "견보탑품(見寶塔品)"의 내포의미를 중심으로 -)

  • Youm, Jung-Seop
    • Journal of architectural history
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    • v.19 no.6
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    • pp.39-59
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    • 2010
  • Seokgatap and Dabotap are representing the tower patterns in "Gyeonbotappum" of the Lotus Sutra. It is very peculiar, for the description on "Gyeonbotappum" is usually made in terms of 'the two Buddhas sitting side by side'. If 'the two Buddhas sitting side by side' is describing the situation in Dabotap, the double structure of Seokgatap and Dabotap can be said to symbolize the scriptural description in a different viewpoint. Its correct comprehension is pretty important in understanding Bulguksa. For this, this paper first arranged the critical minds and flows about the faith objects in Indian Buddhism. And, it was considered how these aspects were accumulated through Saddharma-pundarika sutra. Secondly, it was considered why "Gyeonbotappum" took the typical symbolism in Saddharma-pundarika sutra(Lotus Sutra). These parts should be necessarily considered in advance in that Seokgatap and Dabotap were derived from the form of "Gyeonbotappum". Based on this approach, the author checked the actual aspects of Seokgatap that the tower was built on a natural rock ground and the stones surrounding the tower are constituting the 8-directional Lotus site. With these two aspects, we could get the clue on the foundation time of Bulguksa that its founder had intended. In that Dabotap was formed on the basis of "Gyeonbotappum", the features of Dabotap is very important in comprehending its foundation viewpoint. As a result, the viewpoint of double towers in Bulguksa can be said to be the one that the world of suffering was to change to the Pure Land after Sakyamuni preached the Lotus Sutra on the top of Mt. Grdhrakuta and Prabhutaratna-tathagata proved it. This foundation viewpoint shows us clearly that 'the Lotus Buddhist Country' existed in parallel to the Avatamska Buddhist Country. It secures an appropriate meaning in that it can complement or adjust our understanding on the 'Buddhist country (Bulguk)' of Bulguksa where the Avatamska Idea is emphasized relatively highly as shown in the whole title of Bulguksa as 'Avatamska Bulguksa.'

Isolation and Purification of Protein-bound Polysaccharides from Mycelia of Flammulina velutipes Grown on Sawdust Medium (팽나무버섯 수확후의 톱밥배지로부터 단백다당류의 분리 및 정제)

  • Ha, Hyo-Cheol;Park, Shin;Park, Kyung-Sook;Lee, Shun-Woo;Jung, In-Chang;Kim, Seon-Hee;Kwon, Yong-Il;Lee, Jae-Sung
    • KSBB Journal
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    • v.10 no.5
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    • pp.589-597
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    • 1995
  • Protein-bound polysaccahrides(PBP) were isolated, purified, and characterized from the sawdust media after harvesting the fruit body of Flammulina velutipes. The yield of the crude PBP(Fr.CA) extracted from the sawdust media, was 0.367% relative to the original sawdust media. The total sugar and protein contents of Fr.CA were 19.8% and 23.8% respectively. Using the membrane filtration, the fraction of which the molecular weight is over 300 kDa(Fr.A) was isolated from the Fr.CA and the yield was 44.6% relative to the Fr.CA. This result indicates that high molecular PBP is the dominant components of the Fr.CA. The Fr.A was separated into three fractions (Fr.A-1, Fr.A-2 and Fr.A-3) whose yields are 5.8%, 8.5% and 13.2% respectively. These fractions were further purified using gel filtration, obtaining a single peak in each fraction that considered as pure PBP Among them, the yield of Fr.A-1-${\alpha}$ was 20.9% relative to the Fr.A-1, and the molecular weight was 800 kDa. Monosaccharide components such as glucose, galactose, mannose, xylose and fucose could be detected all fractions by a HPLC analysis. Especially in the Fr.A-1-${\alpha}$ fraction, the content of glucose and galactose appeared to be high.

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