• Journal of the Korean Society of Radiology
• /
• v.15 no.3
• /
• pp.281-291
• /
• 2021

This study aimed to measure the quantitative changes in images according to the use of compressed sensing in expressing the slow flow rate in TOF MRA test using magnetic resonance imaging. This study set different blood flow rate sections by using auto-injector and flow phantom and compared changes in the SNR, CNR, SSIM, and RMSE measurements by different CS factors between TOF with CS and TOF without CS. One-way ANOVA was performed to test the effect on the image induced by the increase of the CS factor. The results revealed that TOF MRA with CS significantly decreased scan time without significantly affecting SNR and CNR compared to TOF MRA with CS. On the other hand, the differences in SSIM and RMSE between TOF with CS and TOF without CS increased as the CS factor increased. Therefore, it is necessary to efficiently reduce scan time by adapting the CS technique while considering the appropriate range of the CS factor. Additionally, more studies are needed to evaluate CS factors and the similarity precision of images further.

• Mass Spectrometry Letters
• /
• v.10 no.3
• /
• pp.84-87
• /
• 2019

Liquid chromatography mass spectrometry is widely employed in proteomics studies. One of such instruments is the Liquid Chromatography (LC)-Matrix-assisted laser desorption ionisation (MALDI)-Time of flight (TOF) or LC-MALDI-TOF/TOF. In this study, this instrument was used to identify the membrane proteins of a protozoan parasite namely Entamoeba histolytica. It causes amoebiasis in human. The E. histolytica trophozoites were cultured prior to the membrane protein extraction using the conventional method, $ProteoPrep^{(R)}$ and $ProteoExtract^{(R)}$ kits. Then, the membrane protein extracts were trypticdigested and analysed by LC-MALDI-TOF/TOF. Approximately, 194 proteins were identified and 27.8% (54) were predicted as membrane proteins having 1 to 15 transmembrane regions and signal peptides by combining all three extraction methods. Also, this study has discovered 3 unique proteins as compared to our previous study which merit further investigation.

• Journal of radiological science and technology
• /
• v.30 no.3
• /
• pp.265-270
• /
• 2007

The purpose of this study was to evaluate the possibility of utilizing MT pulse at CE-TOF-MRA in patients with cerebral infarction. MRA using time-of-flight(TOF) technique with varying offset frequencies (0, 600, 1,200, and 1,800 Hz) magnetization transfer were performed in 10 patients with cerebral infarction at 3.0T MR scanner. CE-TOF-MRA and TOF-SPGR in normal vessel shown decreased SNR and increased CNR. The highest CNR in narrowing vessel shown at CE-TOF-MRA using 600 and 1,200 Hz offset frequencies. CNR in stenosis vessel increased dependent on using offset frequencies. The occlusion was clearly shown, and the highest CNR in occlusion shown at CE-TOF-MRA using 1,800 Hz offset frequencies. There was no shape variation in narrowing vessel or no visualizing vessel.

• Mass Spectrometry Letters
• /
• v.9 no.2
• /
• pp.56-60
• /
• 2018

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly $[a_n+H]^+$, $[z_n+H]^+$, and $[y_n]^+-type$ products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.6 no.2
• /
• pp.134-143
• /
• 2005

Collagen is the important structural proteins in mammals with various peptide composition and cross-linkings. The direct analysis of collagen protein was not suitable because of its structural complexity and diversity. In this study, we suggest the simple way of collagen analysis by introducing matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) to identify the collagen and its trypsin-digested fragments, and by subsequent time-of-flight tandem mass spectrometry(Q-TOF MS/MS) to analyze the amino acid sequences of identified fragments. Using the collagen samples extracted from the tail of mouse, 10 separated bands were found in SDS-PAGE, and the masses of most bands could be more finely determined by MALDI-TOF MS. When each 10 separated proteins was tryptic digested and introduced to MALDI-TOF, the Gly1056-Arg1073 fragment from $\alpha_1$-chain was identified in four bands, and the Gly1056-Arg1073 fragment from $\alpha_2$-chain was identified in five bands, both in type I collagen. Although few fragments were found because of the cross-linkings left in digested collagen sample, it could be determined that the type I collagen existed at least in 7 separated bands. When the amino acid sequences of two identified fragments were analyzed by Q-TOF MS/MS, both sequences were identical with those determined by MALDI-TOF MS. It suggested that the two peaks in MALDI-TOF MS caused by the fragments identified in this work could be used as the fingerprint to simply identify type I collagen in protein samples.

• The KIPS Transactions:PartB
• /
• v.17B no.4
• /
• pp.287-294
• /
• 2010

TOF technique is one of the skills that can obtain the object's 3D depth information. But depth image has low resolution and fingertip occupy very small region, so, it is difficult to find the precise fingertip's 3D information by only using depth image from TOF camera. In this paper, we estimate fingertip's 3D location using Arm Model and reliable hand's 3D location information that is modified by hexahedron as hand model. Using proposed method we can obtain more precise fingertip's 3D information than using only depth image.

• Applied Chemistry for Engineering
• /
• v.28 no.3
• /
• pp.263-271
• /
• 2017

The application of mass spectrometry to polymer science has rapidly increased since the development of MALDI-TOF MS. This review summarizes current polymer analysis methods using MALDI-TOF MS, which has been extensively applied to analyze the average molecular weight of biopolymers and synthetic polymers. Polymer sequences have also been analyzed to reveal the structures and composition of monomers. In addition, the analysis of unknown end-groups and the determination of polymer concentrations are very important applications. Hyphenated techniques using MALDI-tandem MS have been used for the analysis of fragmentation patterns and end-groups, and also the combination of SEC and MALDI-TOF MS techniques is recommended for the analysis of complex polymers. Moreover, MALDI-TOF MS has been utilized for the observation of polymer degradation. Ion mobility MS, TOF-SIMS, and MALDI-TOF-imaging are also emerging technologies for polymer characterization because of their ability to automatically fractionate and localize polymer samples. The determination of polymer characteristics and their relation to the material properties is one of the most important demands for polymer scientists; the development of software and instrument for higher molecular mass range (> 100 kD) will increase the applications of MALDI-TOF MS for polymer scientists.

• The Journal of the Korea Contents Association
• /
• v.10 no.3
• /
• pp.215-221
• /
• 2010

To assess the clinical value of time resolved imaging of contrast kinetics(TRICKS) MRA by comparison with conventional time of flight(TOF) MR angiography. Both TOF-MRA and TRICKS-MRA were performed in 17 patients with cerebrovascular disease and in 6 patients with brain tumor. Among 17 cerebraovascular patients, digital subtraction angiography(DSA) data were also obtained in 11 patients. TOF-MRA showed good spatial resolution but short in temporal resolution. Although TRICKS-MRA showed somewhat low spatial resolution, it showed superior temporal resolution by distinguishing vessel and tumor in all patients. Also, from the analysis of vessel-tumor relationship, TRICKS-MRA showed better performance than TOF-MRA. TRICKS-MRA makes it possible to image arterial, capillary and venous phase sequentially with very speedy manner and therefore, the clinical use of this method is highly suggestive for future use.

• Mass Spectrometry Letters
• /
• v.8 no.1
• /
• pp.14-17
• /
• 2017

The effect of cationization agent concentration on glycan detection via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was investigated using $Na^+$ ions in the form of NaCl as the cationization agent. NaCl solution concentrations ranging from 1 mM to 1 M were investigated. Glycans from ovalbumin were mixed with the cationization agent solution and the 2,5-dihydroxybenzoic acid (2,5-DHB) matrix solution in a volume ratio of 1:1:1. The resulting mixture was loaded onto the MALDI plate. Two MALDI-TOF MS instruments (Voyager DE-STR MALDI-TOF MS and Tinkerbell RT MALDI-TOF MS) were used for detection of glycans. The best detection, in terms of the number of identified glycans, the peak intensity, and the signal-to-noise (S/N) ratio, was obtained with NaCl concentrations of 0.01-0.1 M for both MALDI-TOF MS instruments.

• Journal of Radiation Protection and Research
• /
• v.41 no.2
• /
• pp.141-147
• /
• 2016

Background: A project for building a neutron time-of-flight (nTOF) facility is progressing. We expect that the construction will start in early 2016. Before that, a detailed simulation based on the current architectural drawings was performed to optimize the performance of our facility. Materials and Methods: Currently, several parts had been modified or changed from the original design to reflect requirements such as the layout of the electron beam line, shape of the vacuum chamber producing a neutron beam, and the underground layout of the nTOF facility. Detailed analysis for these modifications has been done with MCNP simulation. Results and Discussion: An overview of our photo-neutron source and KAERI nTOF facility were introduced. The numerical simulations for heat deposition, source term, and radiation shielding of KAERI nTOF facility were performed and the results are discussed. Conclusion: We are expecting that the construction of the KAERI nTOF facility will start in early 2016, and these results will be used as basic data.