Browse > Article
http://dx.doi.org/10.5478/MSL.2019.10.3.84

Analysis of Entamoeba histolytica Membrane via LC-MALDI-TOF/TOF  

Ujang, Jorim Anak (Institute for Research in Molecular Medicine, Universiti Sains Malaysia)
Noordin, Rahmah (Institute for Research in Molecular Medicine, Universiti Sains Malaysia)
Othman, Nurulhasanah (Institute for Research in Molecular Medicine, Universiti Sains Malaysia)
Publication Information
Mass Spectrometry Letters / v.10, no.3, 2019 , pp. 84-87 More about this Journal
Abstract
Liquid chromatography mass spectrometry is widely employed in proteomics studies. One of such instruments is the Liquid Chromatography (LC)-Matrix-assisted laser desorption ionisation (MALDI)-Time of flight (TOF) or LC-MALDI-TOF/TOF. In this study, this instrument was used to identify the membrane proteins of a protozoan parasite namely Entamoeba histolytica. It causes amoebiasis in human. The E. histolytica trophozoites were cultured prior to the membrane protein extraction using the conventional method, $ProteoPrep^{(R)}$ and $ProteoExtract^{(R)}$ kits. Then, the membrane protein extracts were trypticdigested and analysed by LC-MALDI-TOF/TOF. Approximately, 194 proteins were identified and 27.8% (54) were predicted as membrane proteins having 1 to 15 transmembrane regions and signal peptides by combining all three extraction methods. Also, this study has discovered 3 unique proteins as compared to our previous study which merit further investigation.
Keywords
LC-MALDI-TOF/TOF; protein identification; Entamoeba histolytica; membrane;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Teixeira, J. E.; Huston, C. D. Infect. Immun. 2008, 76, 959.   DOI
2 EMD Biosciences, Inc. ProteoExtract$^{TM}$ Native Membrane Protein Extraction Kit Cat. No. 444810: User protocol, Clibiochem:Darmstadt, 2004.
3 Zhang, L.; Lun, Y.; Yan, D.; Yu, L.; Ma, W.; Du, B.; Zhu, X. J. Immunol. Methods, 2007, 321, 80.   DOI
4 Shevchenko, G.; Musunuri, S.; Wetterhall, M.; Bergquist, J. 2012. J. Proteome Res. 2012, 11, 2441.   DOI
5 Speers, A. E.; Wu, C. C. Chem. Rev. 2007, 107, 3687.   DOI
6 Molloy, M. P.; Herbert, B. R.; Walsh, B. J.; Tyler, M. I.; Traini, M.; Sanchez, J. C.; Hochstrasser, D. F.; Williams, K. L.; Gooley, A. A. Electrophoresis 1998, 19, 837.   DOI
7 de Hoffmann, E.; Stroobant, V. Mass spectrometry: principles and applications, John Wiley & Sons Ltd: Chichester, 2007.
8 Tsai, F. J.; Chen, S. Y.; Liu, Y. C.; Liao, H. Y.; Chen, C. J. PloS one 2017, 12, e0181423.   DOI
9 Jones, M. A.; Ng, B. G.; Bhide, S.; Chin, E.; Rhodenizer, D.; He, P.; Losfeld, M. E.; He, M.; Raymond, K.; Berry, G.; Freeze, H. H. Am. J. Hum. Genet. 2012, 90, 363.   DOI
10 Auclair, S. M.; Bhanu, M. K.; Kendall, D.A. Protein Sci. 2012, 21, 13.   DOI
11 Barrett, A. J.; Woessner, J. F.; Rawlings, N. D. Handbook of proteolytic enzymes, Elsevier: London, 2012.
12 Estoppey, D.; Lee, C. M.; Janoschke, M.; Lee, B. H.; Wan, K. F.; Dong, H.; Mathys, P.; Filipuzzi, I.; Schuhmann, T.; Riedl, R.; Aust, T., 2017. Cell Rep. 2017, 19, 451.   DOI
13 Dass, C. Fundamentals of contemporary mass spectrometry, John Wiley & Sons: Hoboken. 2007.
14 Ujang, A. J.; Othman, N. Membrane and cytosolic proteomes by 3 extraction methods to E. histolytica HM-1:IMSS. AmoebaDB database. see https://amoebadb.org/cgi-bin/gbrowse/amoebadbaa/?display_citation=MassSpecPeptides_ehisHM1IMSS_massSpec_Ujang_Membrane_Cytosolic_RSRC
15 Karas, M.; Hillenkamp, F. Anal. Chem. 1988, 60, 2299.   DOI
16 Tanaka, K.; Waki, H.; Ido, Y.; Akita, S.; Yoshida, Y.; Yoshida, T.; Matsuo, T. Rapid Commun. Mass Spectrom. 1988, 2, 151.   DOI
17 Wolff, M. M.; Stephens, W. E. Rev. Sci. Instrum. 1953, 24, 616.   DOI
18 Veenstra, T. Mass spectrometry: the foundation of Proteomics. Proteomics for biological discovery, John Wiley & Sons Ltd: Chichester, 2006.
19 World Health Organization. Epidemiol. Bull. 1997, 18, 13.
20 Ujang, J.; Sani, A. A.; Lim, B. H.; Noordin, R.; Othman, N. Proteomics, 2018, 18, 1700397.   DOI
21 Biller, L.; Matthiesen, J.; Kuehne, V.; Lotter, H.; Handal, G.; Nozaki, T.; Saito-Nakano, Y.; Schuemann, M.; Roeder, T.; Tannich, E.; Krause, E. Mol. Cell. Proteom. 2014, 13, 132.   DOI
22 Ujang, J. A.; Kwan, S. H.; Ismail, M. N.; Lim, B. H.; Noordin, R.; Othman, N. Clin. Proteom. 2016, 13, 33.   DOI