• Korean Journal of Pharmacognosy
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• v.22 no.3
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• pp.171-182
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• 1991

Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were assayed and compared with one another. Five kinds of trace elements including Ca and Fe were determined by atomic absorbance spectroscopy. The more the region came down from the top of the deer horn, the more the ratio of Ca to Fe content (Ca/Fe) was increased. The same increasing tendancy was observed in the case of ash content. Gangliosides were isolated from deer horn by alumina column chromatography and identified by TLC using gangliosides Type II obtained from bovine brain as the standard material. The TLC chromatograms were scanned by dual wavelength TLC scanner, then those TLC profiles were compared. Deer horn from China and New Zealand were analogous but those from Soviet and Alaska were each distinguished in their TLC profiles.

• Korean Journal of Veterinary Service
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• v.20 no.2
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• pp.161-168
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• 1997

This study was undertaken to determined the sulfamethazine residues In urine, serum and muscle of slaughtered pigs(n: 230) by the method of TLC, EEC-4-plate and HPLC. 1. Inhibition diameter characteristics of reference bacterial substance by EEC-4-plate method and antibacterial inhibition zone was appeared sulfonamides in BS pH 7.2 2. Residual sulfamethazine was detceted from 7 serum(3.04%) and 10 urine(4.35%) by TLC test and concentration of residues was higher In urine than serum. 3. Residual sulfamethazine was detected from 9 muscle(3.91%) by EEC-4-plate method. 4. Positive samples by TLC test and EEC-4-plate method were exceed 0.1ppm quantitative analysis from 7 muscle(3.04%).

• Korean Journal of Pharmacognosy
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• v.12 no.3
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• pp.119-124
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• 1981

In our country, in order to cure diseases, a large number of crude drug preparations has been available. Nevertheless, the development of crude drug preparations have been inhibited, because the quality control is not completed so far. Therefore, we have eontinued on studing the quality control method by Zig-zag TLC. profile analysis. The water extract of 'Samyo-Tang' and componental crude drug (Glycyrrhizae Radix, Ephedrae Herba, Armenicae Semen) were developed on Silica gel $60F_{254}\;plate\;(E.\;Merck)$ useing elution solvent. The developed plate were examined useing Dual Wavelength Zig-zag Scanner (Shimadzu). According to the results of the experiment, it could be summarized as follow: 1) Original patterns of TLC profiles of 'Samyo-Tang' componental crude drug and mixing two crude drugs of 'Samyo-Tang' were observed. 2) Original patterns TLC profile of each extract after spraying with 2% ninhydrine were observed. 3) In the extract of addition and subtraction of Ephedrae Herba, peak area of Rf 0.48 and Rf 0.60 were varied quantitatively.

• KSBB Journal
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• v.19 no.4
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• pp.269-273
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• 2004

A simple, fast and reproducible quantitative analysis method for sugar concentration composed in oligosaccharide mixture was developed. Two glass TLC plates were prepared per sample. After dipping one plate into the copper bicinchoninate reagent and the other plate into 5% sulfuric acid solution, both plates were baked in microwave oven until sugar spots were developed or the surface temperature of TLC plate becomes 60 to 70 $^{\circ}C$. The corrective factor values [F value =(the value of total sugar concentration converted as glucose unit/the value of reducing sugar concentration converted as glucose unit)/(polymerization degree of sugar)] of different molecular weight sugars were determined. Within the concentration of 0.25∼1.0 $\mu\textrm{g}$ in each sample loaded, the fructose-F (corrective factor value of fructose) was 0.45, yet for the higher concentration (2.5∼7.5 $\mu\textrm{g}$) fructose-F was 1.0. In case of glucose, in the range of 0.5∼7.5 $\mu\textrm{g}$, glucose-F was same as fructose-F, 1.0. However, as the molecular weight of sugar was increased, the F values were decreased in both maltodextrin and isomaltodextrin oligosaccharides in 0.5∼7.5 $\mu\textrm{g}$ of each sample loaded. Interestingly, F values were equal for the same molecular weight sugars, although the structures were different from each other. Using F value of each sugar, we could determine and compare the exact total sugar concentration of different molecular weight maltooligosaccharide and isomaltooligosaccharide. We also could determine if the unknown sugar was a reducing or non-reducing compound by using optimized TLC with microwave oven method.

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.372-379
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• 1991

A rapid and simple quantitative assay method for aminoglycoside-3'- phosphotransferase (APH(3')) derived from E. coli ATCC 21990 was developed using the thin layer chromatographic densitometry, 3'-phosphorylated kanamycin B (3'-PKMB), product of APH (3') reaction, was separated from reaction mixtures by developing on the silica gel TLC plate with chloroform-methanol-ammonia water (3:4:3). The quantity of the 3'-PKMB was measured by densitometry after color development by ninhydrin method. Densitometric TLC assay for APH (3') was showed a good quantitative result and reproducibility. Sensitivity of this assay was 1.56 nmol of 3'-PKMB and could be analyzed many samples at same time. This method may be applicable for the analysis of inactivating enzymes of aminoglycoside antibiotics.

• Korean Journal of Pharmacognosy
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• v.13 no.4
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• pp.153-156
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• 1982

The essential oil fraction of Chrysanthemum sibiricum Turcz. (Compositae) was analysed by means of TLC and GLC. Utilizing silicagel column chromatography, a blue-color compound was isolated, and purified by preparative TLC. The obtain compound had the same Rf-value on TLC and exactly same UV-visible and IR spectra with that of chamazulene isolated from Matricaria chamomilla L. The composition of the essential oil was also compared with those from flowers of Chrysanthemum indicum L. and Chrysanthemum morifolim Ramatuelia.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.17-23
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• 1987

A crude alkaloidal fraction from white ginseng (Panax.gilfsertg C.A. Meyer) showed over thirteen Dragendorff positive spots by TLC using eluent of $CHCl_3$/MeOH(10 : 1). TLC pattern of white, red and Sanchii ginseng (P. notoginseng) was mostly not different from each other, but, in American ginseng (P. quinqgtefolium),two spots having Rf 0.71 and 0.68 were not detected. An alkaloid component was isolated from white ginseng and identified as $N_9$-formyl-1-methyl-$\beta$-carboline (mp $174^{\circ}C$, $C_13H_10N_2O$).

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.1-7
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• 1980

Less-volatile organic components in leaves from aromatic tobaccos of different varieties, both Oriental and Korean types were isolated and concentrated using a simple apparatus with fewer manipulations. Each less-volatile concentrate was then subjected to spectrophotometric recording in the visible range, to thin-layer chromatographic group separation, and high-performance liquid chromatographic profile analysis. The methods allow detection of significant quantitative differences in visible absorption spectra, TLC patterns, and high resolution HPLC profiles among varieties.

• Korean Journal of Medicinal Crop Science
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• v.13 no.3
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• pp.101-104
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• 2005

To explore the Korea native plants to substitute for St. John's wort, which produce hypericin that use commercially for the treatment of mild to moderate depression, hypericin contents of Hypericum erectum and H. ascyron collected in two mountain of Korea were examined. From TLC and TLC-densitometer analysis of hypericin contents and biosynthetic pattern, hypericin was detected in flower and leaf of H. erectum, but not in all organs of H. ascyron. The hypericin content of H. erectum grown 200 m high hill in Mt. Byoung-pung was higher than that of Mt. Ji-ri 500 m and 1300 m high hill. When the seasonal variation of hypericin contents in H. erectum leaf collected from two regions was investigated, leaf collected from both regions on July was higher than other seasons.

• Food Science and Preservation
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• v.8 no.3
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• pp.338-344
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• 2001

The oxidative stability of cholesterol in tallow heated at different frying temperatures (130$\^{C}$, 150$\^{C}$, and 180$\^{C}$) was studied by identifying cholesterol oxides by thin layer chromatography(TLC). And fatty acid compositions in tallow heated were also measured and compared with cholesterol oxides. Unsaturated fatty acid contents slightly decreased as the heating time increased, whereas saturated fatty acid contents increased This phenomenon became excessive especially by heating to higher temperature. It was found that RF value and spot color of the nonsaponifiable lipids from tallow heated on TLC analysis accorded with the synthetic cholesterol oxides in this experiment. Four kinds of cholesterol oxides were detected in tallow heated for 24 hours at three different temperatures. The oxides were identified as 7-$\alpha$-hydroxycholesterol, 7-$\beta$-hydroxycholesterol, 7-ketocholesterol and cholesterol epoxide. It was found that there was a little difference in oxidative pattern of cholesterol between several heating temperatures.