• Journal of Plant Biotechnology
• /
• v.43 no.3
• /
• pp.347-358
• /
• 2016

Brassinosteroid (BR), a plant steroid hormone, plays key roles in numerous growth and developmental processes as well as tolerance to both abiotic and biotic stress. To understand the biological networks involved in BR-mediated signaling pathways and stress tolerance, we performed comparative genome-wide transcriptome analysis of a constitutively activated BR bes1-D mutant with an Agilent Arabidopsis $4{\times}44K$ oligo chip. As a result, we newly identified 1,091 (562 up-regulated and 529 down-regulated) significant differentially expressed genes (DEGs). The combination of GO enrichment and protein network analysis revealed that stress-related processes, such as metabolism, development, abiotic/biotic stress, immunity, and defense, were critically linked to BR signaling pathways. Among the identified gene sets, we confirmed more than a 6-fold up-regulation of NB-ARC and FLS2 in bes1-D plants. However, some genes, including TIR1, TSA1 and OCP3, were down-regulated. Consistently, BR-activated plants showed higher tolerance to drought stress and pathogen infection compared to wild-type controls. In this study, we newly developed a useful, comprehensive method for large-scale identification of critical network and gene sets with global transcriptome analysis using a microarray. This study also showed that gain of function in the bes1-D gene can regulate the adaptive response of plants to various stressful conditions.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.15 no.2
• /
• pp.113-125
• /
• 2012

This research is aimed at evaluating the land surface characterization of KOMPSAT-3A middle infrared (MIR) data. Airborne Hyperspectral Scanner (AHS) data, which has MIR bands with high spatial resolution, were used to assess land surface temperature (LST) retrieval and classification accuracy of MIR bands. Firstly, LST values for daytime and nighttime, which were calculated with AHS thermal infrared (TIR) bands, were compared to digital number of AHS MIR bands. The determination coefficient of AHS band 68 (center wavelength $4.64{\mu}m$) was over 0.74, and was higher than other MIR bands. Secondly, The land cover maps were generated by unsupervised classification methods using the AHS MIR bands. Each class of land cover maps for daytime, such as water, trees, green grass, roads, roofs, was distinguished well. But some classes of land cover maps for nighttime, such as trees versus green grass, roads versus roofs, were not separated. The image classification using the difference images between daytime AHS MIR bands and nighttime AHS MIR bands were conducted to enhance the discrimination ability of land surface for AHS MIR imagery. The classification accuracy of the land cover map for zone 1 and zone 2 was 67.5%, 64.3%, respectively. It was improved by 10% compared to land cover map of daytime AHS MIR bands and night AHS MIR bands. Consequently, new algorithm based on land surface characteristics is required for temperature retrieval of high resolution MIR imagery, and the difference images between daytime and nighttime was considered to enhance the ability of land surface characterization using high resolution MIR data.

• 한국균학회소식:학술대회논문집
• /
• 2015.05a
• /
• pp.41-41
• /
• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.18 no.2
• /
• pp.59-74
• /
• 2015

The purpose of this study is to analyze reasonable green area ratios for mitigating urban heat island considering various land use types. Land uses of 5 types such as single residential, multi residential, commercial area, public facility, and industrial area were considered. Green areas were extracted from the tree attribution of land cover. Effect of urban heat island was analysed by the surface temperature of ASTER thermal infrared radiance scanned daytime and nighttime. Mitigation effect of green area at daytime was higher than nighttime. Surface temperature of green area was low in single residential at daytime. But the difference of surface temperature by each land use type was small. The effect of surface temperature mitigation of green area was lower in industrial area. The results of reasonable green area ratios for mitigating urban heat island indicate that surface temperature was the lowest with green area ratio of 40~50% in single residential, multi residential, and commercial area at daytime. Surface temperature of nighttime was not changed much by green area ratios. Therefore, the results of this study will be suggested in urban development planning to construct effectively green area for mitigating urban heat island.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.1
• /
• pp.633-638
• /
• 2018

We are constantly hearing about the seriousness of food shortages in North Korea through various media reports. Recently, the severity of the problem has increased, and international organizations and relief organizations have become increasingly concerned. Due to the shortage of food and firewood, residents illegally cut trees in the mountains and, as a result, North Korea has become the third fastest-growing area of forest degradation in Asia. However, since North Korea cannot directly measure the extent of forest degradation, remote sensing techniques using satellite imagery have to be applied. The purpose of this study was to analyze the characteristics of forest change in North Korea, in order to understand the severity of the forest degradation problem. For this purpose, Landsat 5 TM and Landsat 8 OLI TIRS satellite images were acquired and classified. As a result, it was found that the forests have turned into wilderness in the Nampo City and Pyongyang municipalities, while the wasteland has changed into forests in the north of Yanggangdo. In addition, the total forested area of the whole region decreased by $4,166.22km^2$, the residential area decreased by $2,017.03km^2$, and the amount of agricultural land increased by $6,625.74km^2$, which is similar to the amount of forested area lost, although the difference in the overall area of the forests between 2017 and 2006 was small.

• Korean Journal of Remote Sensing
• /
• v.32 no.6
• /
• pp.681-691
• /
• 2016

Using various satellite measurements in UV, visible and IR, diverse algorithms to retrieve aerosol information have been developed and operated to date. Advanced Himawari Imager (AHI) onboard the Himawari 8 weather satellite was launched in 2014 and has 16 channels from visible to Thermal InfRared (TIR) in high temporal and spatial resolution. Using AHI, it is very valuable to retrieve aerosol optical properties over dark surface to demonstrate its capability. To retrieve aerosol optical properties using visible and Near InfRared (NIR) region, surface signal is very important to be removed which can be estimated using minimum reflectivity method. The estimated surface reflectance is then used to retrieve the aerosol optical properties through the inversion process. In this study, we retrieve the aerosol optical properties over dark surface, but not over bright surface such as clouds, desert and so on. Therefore, the bright surface was detected and masked using various infrared channels of AHI and spatial heterogeneity, Brightness Temperature Difference (BTD), etc. The retrieval result shows the correlation coefficient of 0.7 against AERONET, and the within the Expected Error (EE) of 49%. It is accurately retrieved even for low Aerosol Optical Depth (AOD). However, AOD tends to be underestimated over the Beijing Hefei area, where the surface reflectance using the minimum reflectance method is overestimated than the actual surface reflectance.

• Journal of Life Science
• /
• v.18 no.12
• /
• pp.1637-1643
• /
• 2008

This study has investigated whether extracellular HSP90 predisposes vascular smooth muscle cells (VSMCs) to pro-inflammatory phenotype. Exposure of rat aortic smooth muscle cells to HSP90 not only enhanced IL-6 release but also profoundly induced IL-6 transcript via promoter activation. HSP90-induced IL-6 promoter activation was suppressed by dominant-negative forms of Toll-like receptor (TLR)-4 and myeloid differentiation factor 88 (MyD88), but not by dominant-negative-forms of TLR-3 and TIR-domain-containing adapter-inducing interferon-${\beta}$ (TRIF). Curcumin, which inhibits dimerization of TLR-4, also attenuated the IL-6 induction by HSP90. Mutation at the NF-${\kappa}B$- or C/EBP-binding site in the IL-6 promoter region suppressed the promoter activation in response to HSP90. The gene delivery of $I{\kappa}B$ using recombinant adenoviruses and treatment with resveratrol, which inhibit NF-${\kappa}B$ activity, attenuated the HSP90-induced IL-6 release from VSMCs. The present study proposes that extracellular HSP90 would contribute to inflammatory reaction in the stressed vasculature by inducing IL-6 in VSMCs, and that TLR-4 and NF-${\kappa}B$ would play active roles in the process.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.36 no.10
• /
• pp.1189-1193
• /
• 2012

The Miniaturized Fingerprint Imager (MFI) is a slim optical mouse that can be used as an input device for application to wireless portable personnel communication devices such as smartphones. In this study, we have fabricated key optical components of an MFI, including the illumination optical components and imaging lens. An LED beam-shaping lens consisting of an aspheric lens and a Fresnel facet was successfully machined using a diamond turning machine (DTM). A customized V-shaped groove for beam path banding was fabricated by the bulk micromachining of silicon that was coated with aluminum using the shadow effect in thermal evaporation. The imaging lens and arrayed multilevel Fresnel lenses were fabricated by electron beam lithography and FAB etching, respectively. The proposed optical components are extremely compact and have high optical efficiency; therefore, they are applicable to ultraslim optical systems.

• Korean Journal of Microbiology
• /
• v.43 no.3
• /
• pp.166-172
• /
• 2007

ERM proteins transfer the methyl group to $A_{2058}$ in 23S rRNA, which reduces the affinity of MLS (macrolide-lincosamide-streptogramin B) antibiotics to 23S rRNA, thereby confer the antibiotic resistance on micro-organisms ranging from antibiotic producers to pathogens and are classified into monomethyltransferase and dimethyltransferase. To investigate the differences between mono- and dimethyltransferase, tirD, a representative monomethylase gene was cloned in Escherichia coli from Streptomyces fradiae which contains ermSF, dimethylase gene as well to overexpress the TlrD for the first time. T7 promoter driven expression system successfully overexpress tlrD as a insoluble aggregate at $37^{\circ}C$ accumulating to around 55% of the total cell protein but unlike ErmSF, culturing at temperature as low as $18^{\circ}C$ did not make insoluble aggregate of protein into soluble protein. Coexpression of Thioredoxin and GroESL, chaperone was not helpful in turning into soluble protein either as in case of ErmSF. These results might suggest that differences between mono- and dimethylase could be investigated on the basis of the characteristics of protein structure. However, a very small amount of soluble protein which could not be detected by SDS-PAGE conferred antibiotic resistance on E. coli as in ErmSF which was expected from the activity exerted by monmethylase in a cell.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.08a
• /
• pp.221-221
• /
• 2013

Vertical LED (VLED) has been recognized as a way to obtain the high-power LED due to their advantages [1]. However, approximately 4% of the light generated from the active region is extracted, if the light extraction from side walls and back side is neglected because of Fresnel reflection (FR) and total internal reflection (TIR) [2,3]. In this study, the optical simulation of the VLED with the various microstructures was performed. Among them, the microlens having the diameter of 3 ${\mu}m$ and the height of 1.5 ${\mu}m$ shown the best result was chosen, and then, optimized microlens was formed on a GaN template using conventional semiconductor process. Various microstructures were proposed to improve the light extraction efficiency (LEE) of the VLED for the simulation. The LEE was simulated using LightTools based on a Monte Carlo ray tracing. The microstructures with hemisphere, cone, truncated and cylinder pattern having diameter of 3 ${\mu}m$ were employed on the top layer of the VLED respectively. The improvement of the LEE by using the microstructure is 87% for the hemisphere, 77% for the cone, 53% for the truncated, 21% for the cylinder, compared with the LEE of the flat surface at the reflectance of 85%. The LEE was increased by 88% at the height of 1.5 ${\mu}m$, compared with the LEE of the flat surface. We found that the microlens on the top layer is the most suitable for increasing the LEE. In order to apply the proposed microlens on n-GaN surface, we fabricated microlens on a GaN template. A photoresist array having hexagonal-closed packed microlens was fabricated on the GaN template. Then, optimization of etching the GaN template was performed using a dry etching process with ICP-RIE. The dry etching carried out using a gas mixture of Cl2 and Ar, each having a flow rate of 16 sccm and 10 sccm, respectively with RF power of 50 W, ICP power of 900 W and chamber pressure of 2 mTorr was the optimum etching condition as shown in Fig. 2(a).