• Laboraroty Animal Research
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• 제34권4호
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• pp.257-263
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• 2018

Trefoil factor 1 (TFF1, also known as pS2) is strongly expressed in the gastrointestinal mucosa and plays a critical role in the differentiation of gastric glands. Since approximately 50% of all human gastric cancers are associated with decreased TFF1 expression, it is considered a tumor suppressor gene. Tff1 deficiency in mice results in histological changes in the antral and pyloric gastric mucosa, with severe hyperplasia and dysplasia of epithelial cells, resulting in the development of antropyloric adenoma. Here, we generated Tff1-knockout (KO) mice, without a neomycin resistant ($Neo^R$) cassette, using the clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRSIPR/Cas9) system. Though our Tff1-KO mice showed phenotypes very similar to the previous embryonic stem (ES)-cell-based KO mice, they differed from the previous reports in that a reduction in body weight was observed in males. These results demonstrate that these newly established Tff1-KO mice are useful tools for investigating genetic and environmental factors influencing gastric cancer, without the effects of artificial gene insertion. Furthermore, these findings suggest a novel hypothesis that Tff1 expression influences gender differences.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1381-1387
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• 2012

We applied enzymatic hydrolysis and tangential flow filtration (TFF) to purify a novel anticancer peptide from Mytilus coruscus (M. coruscus) and investigated its anticancer properties. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Pepsin hydrolysates, which showed clearly superior cytotoxic activity on prostate cancer cells, were further purified using a flow filtration system using a TFF and consecutive chromatographic methods. Finally, a novel anticancer peptide was obtained, and the sequence was identified as Ala-Phe-Asn-Ile-His-Asn-Arg-Asn-Leu-Leu. The peptide from M. coruscus effectively induced cell death on prostate, breast and lung cancer cells but not on normal liver cells. This is the first report of an anticancer peptide derived from the hydrolysates of M. coruscus.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 춘계종합학술대회
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• pp.406-409
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• 2003

TFF(Thin Film Fille.) 방식의 2$\times$2 OADM을 이용하여 1510nm 파장과 1530nm 파장의 광원을 첨가하여 add/drop시킨 4 파장 광 모듈을 구성하였고 이 광모듈을 채택하여 PCS(personal communication system) 및 셀룰러 (cellular) 이동 통신뿐만 아니라 차세대 이동 통신 방식은 IMT-2000 서비스와 다중 링크 시켜 광선로를 재활용하는 방식의 4 파장 광 트랜시버를 구현하였다. OADM을 이용한 4파장 optical signal stream을 구성하여 1510 nm, 1530 nm, 1550 nm, 1570 nm 파장에서 각각 -1.6 dBm, -1.7 dBm, -5.6 dBm, -5.8dBm의 광출력을 얻었다. 제작된 광 트랜시버의 LD 모듈에 12 V의 바이어스 전압을 사용하여 약 1.5dBm의 광 출력 특성을 얻었으며, PD 모듈의 증폭단에는 two-stage 저 잡음 증폭기를 제작하여 1.66dB의 잡음지수와 25.7 dB의 이득을 얻었다. 또한 S$_{11}$과 S$_{22}$ 그리고 입ㆍ출력 정재파비는 각각 -5.47dB, -20 dB 그리고 3.27 : 1, 1.22 : 1의 결과 값을 얻었다.다.