• Title/Summary/Keyword: TEM Journal

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Trials and Effects of A Learner-centered Creative Training Technique on Undergraduate Education of Medical Record Information Management (의무기록정보관리 교육에서 학습자 중심의 창의적 교수법 적용 및 효과)

  • Chun, Jin-Ho;Yoo, Jin-Yeong
    • Journal of Digital Convergence
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    • v.12 no.3
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    • pp.277-288
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    • 2014
  • The purpose of the study is to investigate the students' learning motive through the application of the learner-centered program Creative Teaching Technique(CTT) conducted by undergraduate school of Medical Record Information Management(MRIM), and to improve learning from the results. A questionnaire survey was carried out that started March to June 2013 among the sixty freshmen college students from the Health Administration Department who participated in the CTT during the 12 weeks training. The main results are as follows. The subjects' cognitive results form CTT were relativiely higher in 'increased voluntary participation(4.03)', 'improved concentration(4.00)', 'increased understanding(3.97)' in order. The effects of the tools used in CTT were higher as well in 'two members in a tem(4.08)', 'three-dimensional tools(4.03)' and 'quiz cards(3.95)' in order. While undergoing CTT, the learners considered reviewing repeatedly the content before starting and finishing as mostly helpful. Concludingly, this learner-centered CTT program identified having positive effects on their participation, concentration and understanding. To maximize the learning effects, development and activating a systematic, continuous and supportive program like this CTT is highly recommended.

Inhibition of Foodborne Pathogens and Spoilage Bacteria and Their Structural Changes by Ethanol Extract of Schizandra chinensis Baillon (오미자 에탄올 추출물에 의한 식품위해성 세균의 증식 억제 및 세포구조 변화)

  • Kim, Se-Ryoung;Kim, Mee-Ra
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.1
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    • pp.109-119
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    • 2012
  • This study analyzed the antibacterial activity of the ethanol extract of Schizandra chinensis Baillon against food pathogenic microorganisms to determine its capabilities as a natural antimicrobial agent. A paper disc diffusion test, minimum inhibitory concentration (MIC) determination, and time-kill assay showed that the ethanol extract strongly inhibits the growth of Listeria monocytogenes, Bacillus cereus, Escherichia coli O157:H7, and Pseudomonas aeruginosa. Release of cytoplasmic ${\beta}$-galactosidase was detected in E. coli, E. coli O157:H7, S. aureus, and P. aeruginosa treated with the ethanol extract. An increase of outer membrane permeability caused by the ethanol extract was also observed. An outward flow of cell constituents was detected in the Gram negative strains treated with the ethanol extract. These results imply that the inner and outer membranes of cells were partially destroyed and cell constituents were released by the treatment of the S. chinensis Baillon ethanol extract. The results of this study indicate that ethanol extract of S. chinensis Baillon evidences a fairly good antibacterial effect.

Pollen morphology of Patrinieae Höck (Valerianaceae) (마타리족(Patrinieae Höck, 마타리과)의 화분형태학적인 연구)

  • Jung, Eun-Hee;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.38 no.2
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    • pp.163-177
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    • 2008
  • Pollen grains of 17 taxa (14 species with two additional subspecies and one variety) of genera Patrinia Juss. and Nardostachys DC. in tribe Patrinieae (Valerianaceae) were studied using light microscope, scanning electron microscope, and transmission electron microscope. Pollen grains are medium ($37.41{\times}43.60{\mu}m$ - $45.65{\times}48.50{\mu}m$) to large ($54.88{\times}59.41{\mu}m$ - $61.70{\times}71.00{\mu}m$) in size, tricolpate (rarely tetracolpate) with the characteristic halo surrounding the aperture. In equatorial view, the pollen is oblate to subprolate, and in polar view, it is mostly circular or rarely 3-lobed. Two major pollen types are recognized on the basis of exine sculpturing patterns; Type I: Exine is composed of echinae together with sparse or dense microechinae, and verrucae shallow or rarely absent (Nardostachys and sections Paleopatrinia, and Monanadropatrinia of Patirinia). - Type II: Exine is composed of massive echinae together with dense microechinae, and prominent verrucae (section Centrotrinia of Patrinia). In TEM sections, columellae are extended from the footlayer into verrucae, and exine thickness is uniform at the pole and equator. Additionally, the infrageneric classification systems of the Patrinieae were evaluated on the basis of the present data.

EXPERIMENTAL STUDY OF PERIPHERAL NERVE REGENERATION BY USING NON-TUBULAR NATURAL CELLULOSE MEMBRANE NERVE CONDUIT (비관형 천연 셀룰로오스막 도관을 이용한 말초신경 재생에 대한 실험적 연구)

  • Kim, Soung-Min;Lee, Jong-Ho;Lee, Suk-Keun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.32 no.4
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    • pp.295-307
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    • 2006
  • Styela clava, called non-native tunicate or sea squirt, is habitat which include bays and harbors in Korea and several sites in the sea faced world. We fabricate cellulose membrane nerve conduit (CMNC) from this native sea squirt skin, and evaluate the capacity of promoting peripheral nerve regeneration in the rat sciatic nerve defect model. After processing the pure cellulose membrane from the sea squirt skin as we already published before, CMNC was designed as a non-tubular sheet with 14 mm length and 4 mm width. Total eleven male Spraque-Dawley rats (12 weeks, weighing 250 to 300g) were divided into sham group (n=2), silicone tube grafted control group (n=3) and experimental group (n=6). Each CMNC grafted nerve was evaluated after 4, 8 and 12 weeks in the experimental group, and after 12 weeks, sciatic function was evaluated with sciatic function index (SFI) and gait analysis, and histomorphology of nerve conduit and the innervated tissues of sciatic nerve were all examined using image analyzer and electromicroscopic methods in the all groups. The regenerated axon and nerve sheath were found only in the inner surface of the CMNC after 4 weeks and became more thicker after 8 and 12 weeks. In the TEM study, CMNC grafted group showed more abundant organized myelinated nerve fibers with thickened extracellular matrix than silicone conduit grafted group after 12 weeks. The sciatic function index (SFI) and ankle stance angle (ASA) in the functional evaluation were $-47.2{\pm}3.9$, $35.5^{\circ}{\pm}4.9^{\circ}$ in CMNC grafted group (n=2) and $-80.4{\pm}7.4$, $29.2^{\circ}{\pm}5.3^{\circ}$ in silicone conduit grafted group (n=3), respectively. And the myelinated axon was 41.59% in CMNC group and 9.51% in silicone conduit group to the sham group. The development of a bioactive CMNC to replace autogenous nerve grafts offers a potential and available approach to improved peripheral nerve regeneration. As we already published before, small peptide fragment derived from the basement membrane matrix proteins of squirt skin, which is a kind of anchoring protein composed of glycocalyx, induced the effective axonal regeneration with rapid growth of Schwann cells beneath the inner surface of CMNC. So the possibilities of clinical application as a peripheral nerve regeneration will be able to be suggested.

The Effects of Nitrogen on Microstructure and Magnetic Properties of Nanocrystalline Fe-Nb-B-N Thin Films (나노결정구조 Fe-Nb-B-N 박막의 미세구조 및 자기적 특성)

  • 박진영;서수정;노태환;김광윤;김종열;김희중
    • Journal of the Korean Magnetics Society
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    • v.7 no.5
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    • pp.250-257
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    • 1997
  • The microstructure and magnetic properties of Fe-Nb-B-N thin film alloys, which produced by rf magnetron sputtering method in $Ar+N_2$ mixed gas atmosphere, were investigated. The $Fe_{70}Nb_{14}B_{11}N_5$ films, annealed at 59$0^{\circ}C$, exhibit soft magnetic properties: $4{\pi}M_s=16.5kG$ , $H_c=0.13Oe$ and ${\mu}_{eff}$ (1~10 MHz)=5, 000. The frequency stability of the Fe-Nb-B-N films has also been found to be good up to 10 MHz. The Fe-Nb-B-N thin film alloys annealed at 59$0^{\circ}C$ consist of three phase; fine crystalline $\alpha$-Fe phase with grain size of about 5~10 nm, Nb-B rich amorphous phase and Nb-nitride precipitates with the size of less than 3 nm. Annealed Fe-Nb-B films have two phases; $\alpha$-Fe grains with the size of about 10 nm and Nb-B rich amorphous phase. The addition of N decreased $\alpha$-Fe grain size due to the precipitation of NbN. The good magnetic properties of the Fe-Nb-B-N film alloys are due to fine $\alpha$-Fe grains resulting from the precipitation of NbN.

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Preparation of Gold Coated Liposomes for CT Contrast Medium (CT 조영을 위한 금 코팅 리포솜의 제조)

  • Wee, Tae In;Jeon, Ye Won;Cho, Young Jae;Cho, Sung Keun;Ha, Jeung;Lee, Jeong Won;Cho, Sun Hang;Han, Hee Dong;Shin, Byung Cheol
    • Journal of the Korean Chemical Society
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    • v.57 no.5
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    • pp.634-639
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    • 2013
  • The use of medical imaging has been increased for diagnosis of cancer or vessel disease. Among the medical imaging, computed tomography (CT) is one of the popular methods, however, which should need administration of contrast medium. Therefore, we developed gold coated liposomes (GCL) as a contrast medium. To coat gold on the liposomal surface, positive charged liposomes was prepared and then negative $Au^-$ can coat on the liposomal surface by electronic interaction. The size of GCL was $154.8{\pm}9.2$ nm and surface charge was $27{\pm}3.2$ mV, respectively. The morphology of GCL was confirmed by electron microscopy (SEM) and transmission electron microscopy (TEM). The coating efficiency of gold was 18%. Chemical procedure for gold coating on liposomal surface was not toxic for cell cytotoxicity by MTT assay. Finally, we demonstrated attractive CT image for GCL. Taken together, the GCL would be useful for various vessel related disease as a contrast medium.

Synthesis of vertically aligned thin multi-walled carbon nanotubes on silicon substrates using catalytic chemical vapor deposition and their field emission properties (촉매 화학 기상 증착법을 사용하여 실리콘 기판위에 수직 정렬된 직경이 얇은 다중층 탄소나노튜브의 합성과 그들의 전계방출 특성)

  • Jung, S.I.;Choi, S.K.;Lee, S.B.
    • Journal of the Korean Vacuum Society
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    • v.17 no.4
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    • pp.365-373
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    • 2008
  • We have succeeded in synthesizing vertically aligned thin multi-walled carbon nanotubes (VA thin-MWCNTs) by a catalytic chemical vapor deposition (CCVD) method onto Fe/Al thin film deposited on a Si wafers using an optimum amount of hydrogen sulfide ($H_2S$) additive. Scanning electron microscope (SEM) images revealed that the as-synthesized CNT arrays were vertically well-oriented perpendicular to the substrate with relatively uniform length. Transmission electron microscope (TEM) observations indicated that the as-grown CNTs were nearly catalyst-free thin-MWCNTs with small outer diameters of less than 10nm. The average wall number is about 5. We suggested a possible growth mechanism of the VA thin-MWCNT arrays. The VA thin-MWCNTs showed a low turn-on electric field of about $1.1\;V/{\mu}m$ at a current density of $0.1\;{\mu}A/cm^2$ and a high emission current density about $2.5\;mA/cm^2$ at a bias field of $2.7\;V/{\mu}m$. Moreover, the VA thin-MWCNTs presented better field emission stability without degradation over 20 hours (h) at the emission current density of about $1\;mA/cm^2$.

Characterizations of Tobacco Mosaic Virus isolated from Chinese Foxglove(Rehmannia glutinosa Libosch) (지황(Rehmannia glutinosa Libosch)에서 분리한 Tobacco Mosaic Virus의 특성)

  • 박준식;최민경;유강열;이귀재
    • Korean Journal of Plant Resources
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    • v.16 no.3
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    • pp.230-237
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    • 2003
  • This study was conducted to investigate the occurrence and characterization of tobacco mosaic virus(TMV) in Chinese foxglove isolated from the field of the Chonbuk province(Jinan, Jangsu, Jeongeup). TMV was detected in all three regions and confirmed positive reaction by ELISA test. In the host range test, Chenopodium amaranticola, Nicotiana glutinosa, N. tabacum cv. 'Bright yellow', N. tabacum cv. 'KY­57, Datura stramonium were locally infected with the virus. The virus produced mosaic symptom on inoculated leaves of N. tabacum cv. 'Samson'. However, Chenopodium quinoa, Glycine max, Raphanus sativus, Cucumis sativus, Cucurbita moschata, Brassica rape and Lycopersion esculentum did not show any symptoms. TMV particles were revealed as a stiff rod shape by transmission electron microscopic(TEM) and measured as 300 nm in length with 18 nm in diameter. Total RNA was extracted from showing symptom loaves infected with TMV and the reverse transcription­polymerase chain reaction (RT­PCR) obtained 531 bp DNA product of RNA with specific primer used. The capsid protein of TMV­RE showed higher amino acid sequence homology(97.7%) with TMV­To than with TMV­P(72.2%). The capsid protein of TMV­152 showed same amino acid sequence homology with TMV­F. The result of comparison of nucleotides sequence homology between TMV­RE strain and other TMV strain showed 94% homology with others except TMV­P(67.3%) and TMV ­ C(68.6%).

Morphological Characteristics of Growth of Rice and Barnyardgrass under Various Cropping Patterns - III. Difference in Stem Surface and Ultrastructures (재배양식(栽培樣式)에 따른 벼와 피의 생장(生長) 및 해부형태학적(解剖形態學的) 차이(差異) - III. 재배양식(栽培樣式)에 따른 벼와 피간(間의) 미세구조(微細構造) 차이(差異))

  • Chon, S.U.;Guh, J.O.;Lee, Y.M.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.144-155
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    • 1994
  • Scanning electron micrography and Transmission electron micrography studies were performed on the surfaces of stem(coleoptiles) of rice and barnyardgrass under various cropping patterns at 5 days after seeding or transplanting. Stem surfaces of rice under dry condition possessed rodlet-like epicuticular waxes, trichomes, stomata and cork cell layer in both broadcast and drilled rice while barnyardgrass had only thread-like epicuticular waxes, but trichomes, stomata and cork cell layers were not found. However, rice under water condition has no rodlet-like waxes, stomata and cork cells, and barnyardgrass was similar to that under dry condition. On the other hand, those on stems of 8-day and 25-day transplanted rice were well arranged and developed compared with those of direct seeded rice. Electron micrography showed significant difference in thickness of outer epidermal cell walls of rice and barnyardgrass under dry, water and transplanting condition. Thickness of outer epidermal cell was greater in transplanted rice, dry direct seeded rice, water-direct seeded rice and barnyardgrass in descending order.

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Mineralogical and Geochemical Characteristics of Soils of Barton Peninsula, King George Island, South Shetland Islands, West Antarctica (서남극 사우스셰틀랜드 킹조지섬 바톤반도 육상 토양의 광물학적, 지화학적 특성)

  • Jung, Jaewoo;Koo, Taehee;Yang, Kiho;Kim, Jinwook
    • Journal of the Mineralogical Society of Korea
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    • v.30 no.1
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    • pp.21-29
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    • 2017
  • Surface soils on Barton Peninsula, King George Island, West Antarctica were investigated to acquire the mineralogical and geochemical data of soil in Antarctica. Multiline of techniques for example, X-ray diffraction (XRD), transmission electron microscopy (TEM)-electron energy loss spectroscopy (EELS), and wet chemistry analysis were performed to measure the composition of clay minerals, Fe-oxidation states, cation exchange capacity, and total cation concentration. Various minerals in sediments such as smectite, illite, chlorite, kaolinite, quartz and plagioclase were identified by XRD. Fe-oxidation states of bulk soils showed 20-40% of Fe(II) which would be ascribed to the reduction of Fe in clays as well as Fe-bearing minerals. Moreover, redox states of Fe in smectite structure was a ~57% of Fe(III) consistent to the values for the bulk soils. The cation exchange capacity of bulk soils ranged from 100 to 300 meq/kg and differences were not significantly measured for the sampling locations. Total cations (Mg, K, Na, Al, Fe) of bulk soils varies, contrast to the heavy metals (Co, Ni, Cu, Zn, Mn). These results suggested that composition of bed rocks influenced the distribution of elements in soil environments and soils containing clay compositions may went through the bio/geochemical alteration.