• Title/Summary/Keyword: TCS System

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Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System

  • Ganesh, Irisappan;Vidhya, Selvamani;Eom, Gyeong Tae;Hong, Soon Ho
    • Journal of Microbiology and Biotechnology
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    • v.27 no.6
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    • pp.1106-1111
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    • 2017
  • Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.

Heavy Metal Detection and Removal in Artificial Wastewater Using Two-Component System Based Recombinant Bacteria (Two-component System 기반 재조합균을 이용한 인공폐수에서의 중금속 인지 및 제거)

  • Ravikumar, Sambandam;Hong, Soon-Ho;Yoo, Ik-Keun
    • Korean Journal of Microbiology
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    • v.48 no.3
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    • pp.187-191
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    • 2012
  • Two-component system (TCS)-based bacterial zinc and copper biosensors, in which green fluorescent protein (GFP) is expressed under the control of zraP and cusC promoter in ZraS/R and CusS/R TCS, were evaluated in artificial wastewater. Bacterial biosensors developed in this study efficiently expressed GFP by the recognition of $Zn^{2+}$ and $Cu^{2+}$ in artificial wastewater. Secondly, TCS-based zinc and copper removing bacteria with the peptide displayed on cell surface were examined in artificial wastewater. Zinc and copper removing bacteria expressed the peptide as a fusion protein such as OmpC-ZBP (zinc binding peptide) and OmpC-CBP (copper binding peptide) on the cell surface when sensing exogenous $Zn^{2+}$ and $Cu^{2+}$ through ZraS/R and CusS/R TCS. The recombinant cell expressing metal-adsorbing peptide could efficiently remove copper and zinc (15 and 18 mg/g dry cell weight, respectively) in artificial wastewater. Therefore, it was demonstrated that the TCS-based recombinant cell for the recognition or removal of heavy metal functions well in artificial wastewater environment.

Non-Ionic Surfactants Antagonize Toxicity of Potential Phenolic Endocrine-Disrupting Chemicals, Including Triclosan in Caenorhabditis elegans

  • Alfhili, Mohammad A.;Yoon, Dong Suk;Faten, Taki A.;Francis, Jocelyn A.;Cha, Dong Seok;Zhang, Baohong;Pan, Xiaoping;Lee, Myon-Hee
    • Molecules and Cells
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    • v.41 no.12
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    • pp.1052-1060
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    • 2018
  • Triclosan (TCS) is a phenolic antimicrobial chemical used in consumer products and medical devices. Evidence from in vitro and in vivo animal studies has linked TCS to numerous health problems, including allergic, cardiovascular, and neurodegenerative disease. Using Caenorhabditis elegans as a model system, we here show that short-term TCS treatment ($LC_{50}$: ~0.2 mM) significantly induced mortality in a dose-dependent manner. Notably, TCS-induced mortality was dramatically suppressed by co-treatment with non-ionic surfactants (NISs: e.g., Tween 20, Tween 80, NP-40, and Triton X-100), but not with anionic surfactants (e.g., sodium dodecyl sulfate). To identify the range of compounds susceptible to NIS inhibition, other structurally related chemical compounds were also examined. Of the compounds tested, only the toxicity of phenolic compounds (bisphenol A and benzyl 4-hydroxybenzoic acid) was significantly abrogated by NISs. Mechanistic analyses using TCS revealed that NISs appear to interfere with TCS-mediated mortality by micellar solubilization. Once internalized, the TCS-micelle complex is inefficiently exported in worms lacking PMP-3 (encoding an ATP-binding cassette (ABC) transporter) transmembrane protein, resulting in overt toxicity. Since many EDCs and surfactants are extensively used in commercial products, findings from this study provide valuable insights to devise safer pharmaceutical and nutritional preparations.

Evaluation of Irritating Potential of Newly Developed Toothpaste in the Hamster Oral Mucous Membrane

  • Kim, Bae-Hwan;Kim, Jin-Woo;Chang, Ih-Seop;Sim, Young-Chul;Lee, Yong-Soon
    • Toxicological Research
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    • v.17 no.3
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    • pp.167-171
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    • 2001
  • Oral mucous membrane test using Syrian hamsters was performed to evaluate the reliability as a model system for the assessment of the potentially irritating substances intended for the mucous membranes, and to determine the irritating potential of a new emulsion-type formulated toothpaste. After test substances were implanted into the cheek pouches of hamsters with diluents (20 mg/kg) under pento-barbital sodium anesthesia, we made the comparison in irritation between emulsion-type and dispersion-type of triclosan (TCS) formulations in the range of 0.2% to 0.3%. The emulsion-type formulations using non-ionic surfactant showed less mucosal lesion than other commercial toothpastes with 0.3% TCS, or dispersion-type ones. However, no significant difference in irritation was detected between 0.2% and 0.3% TCS. We report that this hamster cheek pouch method could be a reliable approach for the evaluation slight difference in the irritating potentials of cosmetics and hygiene products intended for the lips or other mucous membranes, and this method showed that the new emulsion-type formulation significantly lowered the TCS-induced toxicity, compared with other commercial toothpastes.

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The Seasonal Forecast Characteristics of Tropical Cyclones from the KMA's Global Seasonal Forecasting System (GloSea6-GC3.2) (기상청 기후예측시스템(GloSea6-GC3.2)의 열대저기압 계절 예측 특성)

  • Sang-Min Lee;Yu-Kyung Hyun;Beomcheol Shin;Heesook Ji;Johan Lee;Seung-On Hwang;Kyung-On Boo
    • Atmosphere
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    • v.34 no.2
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    • pp.97-106
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    • 2024
  • The seasonal forecast skill of tropical cyclones (TCs) in the Northern Hemisphere from the Korea Meteorological Administration (KMA) Global Seasonal Forecast System version 6 (GloSea6) hindcast has been verified for the period 1993 to 2016. The operational climate prediction system at KMA was upgraded from GloSea5 to GloSea6 in 2022, therefore further validation was warranted for the seasonal predictability and variability of this new system for TC forecasts. In this study, we examine the frequency, track density, duration, and strength of TCs in the North Indian Ocean, the western North Pacific, the eastern North Pacific, and the North Atlantic against the best track data. This methodology follows a previous study covering the period 1996 to 2009 published in 2020. GloSea6 indicates a higher frequency of TC generation compared to observations in the western North Pacific and the eastern North Pacific, suggesting the possibility of more TC generation than GloSea5. Additionally, GloSea6 exhibits better interannual variability of TC frequency, which shows relatively good correlation with observations in the North Atlantic and the western North Pacific. Regarding TC intensity, GloSea6 still underestimates the minimum surface pressures and maximum wind speeds from TCs, as is common among most climate models due to lower horizontal resolutions. However, GloSea6 is likely capable of simulating slightly stronger TCs than GloSea5, partly attributed to more frequent 6-hourly outputs compared to the previous daily outputs.

Analysis of Diversion Rate using Expressway Traffic Data(FTMS, TCS): Focusing on Maesong~Balan IC at Seohaean Expressway (고속도로 교통데이터(FTMS, TCS)를 이용한 경로전환율 분석: 서해안고속도로 매송~발안 구간을 중심으로)

  • Ko, Han-Geom;Choi, Yoon-Hyuk;Oh, Young-Tae;Choi, Kee-Choo
    • Journal of Korean Society of Transportation
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    • v.30 no.3
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    • pp.31-41
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    • 2012
  • Due to growing interests in the distribution of traffic volume through information dissemination such as VMS and traffic broadcasting system, the research on the driver's reaction and effect of the traffic report has continued. In this study, we propose a methodology, which estimates the traffic volume of diversion and the consequential diversion rate using FTMS data and TCS data, and the estimation is based on the analysis of the national highway and IC, in which real-time FTMS and TCS data are established. We also calculate the diversion rate of actual targeted sections and analyze the changes in time and spatial diversion rate. In this study, we define a deviation (considering a deviation due to dynamic properties of traffic conditions) found when the outflow traffic volume is temporarily higher than the average outflow traffic volume on a relevant time slot after providing traffic information. The diverting volume is considered to be caused by the traffic information, and the study determines the ratio of traffic volume on highways to that of route diversion as the diversion rate. The analysis on changes in the diversion rate in accordance with the time flow, the initial change in the diversion rate on upstream IC that first acquires the report on the traffic congestion is significant. After that, the change in the diversion rate on upstream IC affects the route diversion on downstream IC with spatial and time flow, and this again leads the change in upstream IC. Thereby, we confirmed that there is a feedback-control circulation system in the route diversion.

An Approach for Estimating Traffic-Zonal Origin-Destination Matrices(O-D) from Toll Collection System's Ones (고속도로 영업소간 기.종점통행량으로부터 교통죤간 기.종점통행량 추정기법 연구)

  • 신언교;황부연;신승원
    • Journal of Korean Society of Transportation
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    • v.17 no.1
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    • pp.7-17
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    • 1999
  • The expressway network includes a total of about 1,899 km in our country The only 1,016 km of that is being managed by the closed Toll Collection System(TCS) which is composed of 74 tollgates. We obtain inter-tollgate O-D matrices from that system everyday. But, they are not traffic-zonal O-D matrices. So they have not been used for the expressway traffic analysis and the traffic demand estimation despite of their accuracy. If we could estimate the traffic-zonal O-D matrices from TCS O-D ones, we could perform expressway traffic analysis more efficiently. Moreover we could obtain more precise expressway O-D matrices and traffic-zonal O/D ones by this approach than by the conventional ones. In this paper. we proposed the model estimating traffic-zonal O/D matrices from TCS O-D ones. The assigned volumes with the estimated traffic-zonal O-D matrices produced the only 17.9% error all over the TCS expressway section when compared to the real traffic volumes. So, the proposed model enables for us to estimate more accurate O/D matrics than any other existing methods.

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Power Electronics System of E-TCS(Electric Turbo Compounding System) for Construction Equipment (건설기계용 Electric Turbo Compounding System(E-TCS)의 Power Electronics System)

  • Park, Chanheung;Choi, Hyuntae
    • Proceedings of the KIPE Conference
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    • 2016.11a
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    • pp.159-160
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    • 2016
  • 본 논문에서는 최근 건설기계용으로 연구되고 있는 E-TCS의 Power Electronics System에 대한 Topology와 제어방법을 제안한다. 디젤엔진과 같이 사용되는 E-TCS는 압축기와 터빈 그리고 요구되는 회전속도로 고속회전 및 발전이 가능한 모터와 이를 제어하는 인버터로 구성된다. 기존의 E-TCS를 위한 Power Electronics System의 경우 배터리의 전압강압으로 인버터의 전류량이 증가하여 Power Electronics System 및 모터의 Volume이 커지는 단점이 있으며, 특허가 존재하여 사용이 어렵다. 본 논문에서는 이와 같은 단점을 개선한 E-TCS를 위한 새로운 Topology 구성 및 제어방식을 제안한다.

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3D-culture models as drug-testing platforms in canine lymphoma and their cross talk with lymph node-derived stromal cells

  • An, Ju-Hyun;Song, Woo-Jin;Li, Qiang;Bhang, Dong-Ha;Youn, Hwa-Young
    • Journal of Veterinary Science
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    • v.22 no.3
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    • pp.25.1-25.16
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    • 2021
  • Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor. Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs). Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system. Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qp, MRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells. Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.

A study on interoperability and applicability of the Korean Radio based Train Control System(KRTCS) (무선기반 도시철도용 열차제어시스템(KRTCS)의 상호운영성 및 적용성 연구)

  • Kang, Yong-Sam
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.6
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    • pp.1095-1102
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    • 2016
  • A TCS(Train Control System) is the core facility to ensure the safety and efficiency of train operations, it is difficult to have interoperability between the TCS suppliers. To ensure the interoperability between TCSs, there is a need for definition, validation and standardization on the system configuration, the functions of subsystems, the interface between subsystems an the system protocol. In this paper, we analyzed the completeness of the KRTCS(Korean Radio based Train Control System) specification, derived supplements what you need to apply the KRTCS specification to new urban lines.