• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6047-6053
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• 2012

Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations ($200{\mu}g/mL$) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and $30{\mu}g/mL$) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

• 한국재료학회지
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• 제11권1호
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• pp.44-47
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• 2001

Ni 전극을 사용하는 $BaTiO_3$계 내환원성 X7R 조성에서 $Er_2$$O_3$ 첨가가 유전특성에 미치는 영향에 대하여 환원성 분위기에서 연구하였다. $MnO_2-MgO$가 첨가된 내환원조성에서 첨가량이 조절된 $Er_2O_3$의 복합첨가로 유전율의 온도안정성이 향상되어 X7R 규격을 만족시켰으며 2,970 이상의 상온 유전상수와 1.0% 이하의 유전손실율이 관찰되었다. $Er_2O_3$가 3.0 mol% 이상으로 과량 첨가되었을 경우 유전체의 온도특성은 향상되었으나 상온 유전상수가 현저히 감소하였다. 다른 첨가조성(1.5 mol% $Er_2O_3$2.0 mol% MgO)이 고정될 때 TCC곡선은 $MnO_2$첨가량이 증가함에 따라 시계방향으로 회전하였으며, 온도안정성을 향상시켰다.

• 대한수의학회지
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• 제44권2호
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• pp.225-231
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• 2004

This study was conducted to determine the incidence of microorganisms associated with dressed broiler with intact skin and without skin at different frozen storage periods such as 0, 10, 20, 30 days and to demonstrate the role of packaging and pretreatment chilling on the changes of carcass quality. The values of total viable count (TVC), total coliform count (TCC), total streptococcal count (TStC) and total staphylococcal count (TSC) were determined for meat samples of thigh and breast and swab samples of visceral surfaces of the broilers with intact skin and without skin. It was observed that the values of TVC, TCC, TStC and TSC in both cases of dressed broiler with intact skin and without skin exceeded the International Commission on Microbiological Specification for Foods. However, numbers of microorganisms were considerably decreased during the frozen storage. Packing and prechilled conditions were generally better effective in decrease of the loads of microorganisms than without packing and prechilled conditions, and lower bacterial numbers were also found in dressed broiler with intact skin than that without skin. The highest sensory panel score was obtained at 10 days of frozen storage. These results, thus, indicate that usages of appropriate periods and conditions of frozen storage and packaging systems can minimize the potential health hazards associated with contaminants gaining access to the dressed or processed broilers and improve the quality and shelf life of dressed broilers.

• 한국동물위생학회지
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• 제30권2호
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• pp.191-196
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• 2007

The slaughter process for cattle will inevitably transfer some bacteria onto the carcasses. The goal of food safety programs is to minimize and effectively remove this contamination. This study was attempted by the Verif $EYE^{TM}$ machine-vision technology that might be useful for reducing microbial indicator counts and could reduce the contamination chance of E coli O157:H7 and Salmonella spp on beef carcasses. For the evaluation of the effectiveness of the Verif $EYE^{TM}$ technology, 80 samples were examined by the inspection device over 15 days. On an examination of FDS-positive samples compared to negative controls from the same carcasses, aerobic plate counts were bigger than the negative control samples (5.26 vs 4.60 log). Enterobacteriaceae counts were greater on the positive samples than the corresponding negative control samples (2.07 vs 1.17log). There was a consistent correlation between samples detected by the Verif $EYE^{TM}$ system with detectable counts. For example, 100% of positive samples had detectable APC and 91.2% of positive samples had detectable TCC. Therefore, if areas detected as positive for contamination by the Verif $EYE^{TM}$ system were removed from the carcasses, significant sources of microbial contamination will be reduced for objective compliance with HACCP. This results suggest that the use of Verif $EYE^{TM}$ machine-vision technology might be useful for reducing microbial indicator counts (APC, TCC) and could help reduce the risk of presence of E coJi O157:H7 and Salmonella spp on Beef carcasses.

• 한국건설관리학회논문집
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• 제10권3호
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• pp.139-147
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• 2009

Accurate construction cost estimation in the initial stage of building project plays a key role for project success and for mitigation of disputes. Total construction cost(TCC) estimation of apartment projects in Vietnam has become more important because those projects increasingly rise in quantity with the urbanization and population growth. This paper presents the application of artificial neural networks(ANNs) in estimating TCC of apartment projects. Ninety-one questionnaires were collected to identify input variables. Fourteen data sets of completed apartment projects were obtained and processed for training and generalizing the neural network(NN). MATLAB software was used to train the NN. A program was constructed using Visual C++ in order to apply the neural network to realistic projects. The results suggest that this model is reasonable in predicting TCCs for apartment projects and reinforce the reliability of using neural networks to cost models. Although the proposed model is not validated in a rigorous way, the ANN-based model may be useful for both practitioners and researchers. It facilitates systematic predictions in early phases of construction projects. Practitioners are more proactive in estimating construction costs and making consistent decisions in initial phases of apartment projects. Researchers should benefit from exploring insights into its implementation in the real world. The findings are useful not only to researchers and practitioners in the Vietnam Construction Industry(VCI) but also to participants in other developing countries in South East Asia. Since Korea has emerged as the first largest foreign investor in Vietnam, the results of this study may be also useful to participants in Korea.

• 한국축산식품학회지
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• 제32권1호
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• pp.40-44
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• 2012

To develop a new starter culture for Mozzarella cheese, the immunomodulatory action of Streptococcus macedonicus LC743 in male C57BL/6 mice was studied. Mice were fed for 7 wk with feed containing 1% Mozzarella cheese made with three kinds of starter cultures from S. macedonicus LC743 (G3), FD-DVS TCC-3 (G2) and S. macedonicus LC743 : FD-DVS TCC-3(1:1) (G4) and control (feed only, G1), respectively. No significant differences in body weight gain were observed among the various groups of mice. The spleen index and thymus index were observed and no significant differences were found among the groups. The production of TNF-${\alpha}$ of S. macedonicus LC743 group significantly increased compared to the control group. The production of IL-$1{\beta}$ was significantly enhanced by the feeding of S. macedonicus LC743 group compared to the control group. In regards to the white blood cell counts, the neutrophil percentages were significantly higher in the G1 group compared to other groups. The lymphocyte percentages were significantly higher in G2, G3 and G4 groups in comparison to the control group. The results of this study may suggest that the supplementation of S. macedonicus LC743 can increase the cytokine production activity by the activated macrophages in mice. Based on the result of this study, it could be concluded that S. macedonicus LC743 could stimulate the immune functions of mice.

• Journal of Microbiology
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• 제45권4호
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• pp.291-296
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• 2007

Insoluble glucans synthesized by Streptococcus mutans enhance the pathogenicity of oral biofilm by promoting the adherence and accumulation of cariogenic bacteria on the surface of the tooth. The objective of this study was to investigate the effect of Leuconostoc spp. on the in vitro formation of S. mutans biofilm. Three strains, Leuconostoc gelidum A TCC 49366, Leuconostoc mesenteroides ssp. cremoris A TCC 19254 and Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, were used in this study. They exhibited profound inhibitory effects on the formation of S. mutans biofilm and on the proliferation of S. mutans. The water-soluble polymers produced from sucrose were most strongly produced by L. gelidum, followed by L. mesenteroides ssp. cremoris and L. mesenteroides ssp. mesenteroides. The mean wet weights of the artificial biofilm of S. mutans were also significantly reduced as a result of the addition of the water-soluble polymers obtained from Leuconostoc cultures. According to the results of thin-layer chromatographic analysis, the hydrolysates of the water-soluble polymers produced by Leuconostoc were identical to those of dextran T-2000, forming predominately ${\alpha}-(1-6)$ glucose linkages. These results indicate that dextran-producing Leuconostoc strains are able to inhibit the formation of S. mutans biofilm in vitro.

• 한국전기전자재료학회논문지
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• 제21권5호
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• pp.440-446
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• 2008

To manufacture the MLCC with X7R for high voltage stability, $BaTiO_3-MgO-MnO_2-Y_2O_3$ with $(Ba_{0.4}Ca_{0.6})SiO_3$ glass frit was formulated. Based on this composition, the addition of $Er_2O_3$ showed that TCC(Temperature Coefficient Capacitance) at $85^{\circ}C$ was improved from 5 % to ${\sim}0\;%$, but the dielectric constant and IR (Insulation Resistance) were decreased. The glass frit improved the dielectric constant and IR, so the appropriate contents of $Er_2O_3$ and glass frit were 0.6 mol% and 1 wt%, respectively. It showed that the dielectric constant and RC constant were 2,550 and 2,000 (${\Omega}F$), respectively in the sintering condition at $1250^{\circ}C$ in PO2 $10^{-7}$ Mpa. The MLCC with $3.2{\times}1.6$ (mm) size and $1\;{\mu}F$ was also suited for X7R with the above composition.

• 대기
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• 제28권4호
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• pp.479-490
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• 2018

This study assesses the prediction skill of regional scale model for the mean temperature anomaly over South Korea produced by Pusan National University Coupled General Circulation Model (PNU CGCM)-Weather Research and Forecasting (WRF) chain. The initial and boundary conditions of WRF are derived from PNU CGCM. The hindcast period is 11 years from 2007 to 2017. The model's prediction skill of mean temperature anomaly is evaluated in terms of the temporal correlation coefficient (TCC), root mean square error (RMSE) and skill scores which are Heidke skill score (HSS), hit rate (HR), false alarm rate (FAR). The predictions of WRF and PNU CGCM are overall similar to observation (OBS). However, TCC of WRF with OBS is higher than that of PNU CGCM and the variation of mean temperature is more comparable to OBS than that of PNU CGCM. The prediction skill of WRF is higher in March and April but lower in October to December. HSS is as high as above 0.25 and HR (FAR) is as high (low) as above (below) 0.35 in 2-month lead time. According to the spatial distribution of HSS, predictability is not concentrated in a specific region but homogeneously spread throughout the whole region of South Korea.

• 한국임상수의학회지
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• 제39권1호
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• pp.16-22
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• 2022

A 12-year-old spayed female beagle dog was presented with pollakiuria and stranguria. Abdominal ultrasonography identified irregular a marginated, hyperechoic mass in the urethra and trigon area of the bladder. Computed tomography (CT) revealed a heterogeneous mass in the trigone area leading to a urethra. There was no evidence of regional or distant metastasis. Cytologic analysis suspected transitional cell carcinoma (TCC). The patient was treated with piroxicam, mitoxantrone, and once weekly fractionated radiation therapy (RT) with volumetric modulated arc therapy (VMAT). A follow-up CT scan at 6 months after RT revealed a reduction in tumor size. At 17 months after the start of RT, the patient became severely anorectic and lethargic. Ultrasound examination revealed a hyperechoic mass in the apex area of bladder while the trigone area of the bladder and urethra appeared normal. Multiple hypoechoic nodules of various sizes were found in the liver and spleen. The patient was humanely euthanized at the request of the owner. A combination of piroxicam, mitoxantrone, and hypofractionated RT with VMAT protocol was well tolerated. This case described tumor response and survival time of a canine TCC treated with piroxicam, mitoxantrone, and once weekly palliative RT using computer-assisted planning and VMAT.