• Title/Summary/Keyword: TCA

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trans-Cinnamaldehyde-Induced Apoptosis in AGS Cells (AGS 세포주에서 트랜스 신남알데하이드의 세포 사멸 유도)

  • Lee, Sunyi;Jung, Joohee
    • Journal of Food Hygiene and Safety
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    • v.36 no.1
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    • pp.100-104
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    • 2021
  • trans-Cinnamaldehyde (TCA), as one of the active ingredients in cinnamon, has been reported to have antiviral, antibacterial and antifungal effects as well as anti-cancer effects in several cancer cell lines. However, reports of TCA in gastric cancer are rare, and its mechanism is unclear. In this study, we investigated the anti-proliferative effect of TCA and its mechanism in gastric cancer AGS cells. TCA dose-dependently inhibited the cell viability of AGS cells. Our results suggested that TCA induces apoptosis through changes in cell morphology. To elucidate its mechanism, we investigated the expression level of apoptosis-related proteins. TCA induced the expression of p53 and Bax proteins, and then increased the cleaved caspase 9 and cleaved PARP. These results indicated that TCA triggers apoptosis via p53 pathway in AGS cells. Our results suggested that TCA might be a new anticancer drug candidate for gastric cancer.

Development of TCA Method for Cost Analysis in Farmhouse (TCA 분석 TOOL 개발과 농가비용 비교분석)

  • Yoon, Sung-Yee
    • Korean Journal of Organic Agriculture
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    • v.12 no.1
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    • pp.23-44
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    • 2004
  • TCA(Total Cost Assessment) can be defined by a tool to evaluate actual profit about investment. Also, It can be used by method to make more reasonable decision-making in business. Enforcement of 'direct payment system' for environment friendship farmhouse of government is spreading recent times. But, it is true that it is no definite standard about appropriation of disbursement amount of money. TCA method can be utilized usefully for solution of these problem. Also, It will help to decide price grasping invisible cost and environment cost. Therefore, We tried that may can apply TCA theory in farmhouse class, and attempted economic performance estimation which use this. This study introduces TCA theory and developed applicable expense list in agriculture field. Also, We applied TCA theory to farmhouse. This theories referenced domestic and th6 foreign countries, connection literature. But, It handled part about invisible cost and social cost etc..

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Glycomacropeptide Hydrolysed from Bovine K-Casein ; II. Chromatographic Changes of k-Casein Macropetide as Related to Trichloroacetic Acid Concentration (우유의 k-Casein에서 분해된 Glycomacropeptide에 관한 연구; II. Trichloroacetic Acid의 농도에 따른 k-Casein Macropeptide 분별 특성의 변화)

  • Moon Yong-Il;Lee Wonjae;Oh Sejong
    • Food Science of Animal Resources
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    • v.25 no.4
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    • pp.478-482
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    • 2005
  • Bovine k-casein macropeptide was prepared by adding TCA (3, 6, and $12\%$) treatment after chymosin reaction. Each TCA soluble macropeptide was fractionated into five peak by ion exchange column chromatography. In proportiion to TCA concentrations, the ratio of peak area showed different the elution pattern. At the 6 and $12\%$ TCA concentration, area ratio of P-I which did not content carbohydrates was decreased to 19.9 and $17.0\%$ of total peak area respectively. The area of P-III was changed from $10.2\%\;to\;26.2\;and\;13.2\%$ when the TCA concentration was increased from 3 to 6 and $12\%$ Cholera toxin binding activity of k-casein macropeptide eluted at $0.17\~0.18M$ NaCl gradient was not inhibited by 6 and $12\%$ TCA treatments. The use of $6\%$ TCA as extraction buffer was feasible and led to an effective separation of the peak III.

Screening of Zero-Valent Metal for the Removal of High Concentration PCE and 1,1,1 TCA (고농도 PCE 및 1,1,1 TCA 제거를 위한 영가금속 선정)

  • Kwon, Soo-Youl;Kim, Young
    • Journal of Wetlands Research
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    • v.12 no.1
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    • pp.23-31
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    • 2010
  • Chlorinated aliphatic hydrocarbons (CAHs) such as tetrachloroethylene (PCE), 1,1,1-trichloroethane (1,1,1-TCA) are the contaminants most frequently found in soil and groundwater. They have a potential to be toxic to and persistent in environment. This study is focused on selection of zero-valent metal and ores for the removal of high concentration PCE or 1,1,1-TCA and mixture of two compound. For the screening of suitable metals, we measured dechlorination rate, removal capacities and economics by using batch reactor test. This results suggest that removal rate and dechlorination of high quality iron and zinc are higher than slag and nature ores like zinc and manganese. Among nature ores, zinc ores(64% purity) have highest removal capacities. And in economics zinc ores is 10 times better than high quality metal tested. We conclude zinc ore is most suitable metal for the removal of PCE or 1,1,1-TCA.

Perspectives on Advanced TCA Technology in BcN (BcN에서의 Advanced TCA 기술 전망)

  • Lee, Y.K.;Kim, B.T.
    • Electronics and Telecommunications Trends
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    • v.19 no.6 s.90
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    • pp.24-33
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    • 2004
  • Advanced TCA 표준플랫폼은 PICMG 컨소시엄이 주관, 100여 개 회사가 참여하여 앞으로의 새로운 시장확보를 위하여 적극적으로 추진하고 있다. 이러한 표준플랫폼은 실질적인 통신사업자 요구사항을 수용하고 가격을 낮추고 새로운 서비스의 도입에 위험부담을 최소화 할 수 있는 전략을 기반으로 하고 있다. 특히, 우리나라와 같은 상황에서는 개방형 표준 플랫폼을 기반으로 각각의 모듈 별 기술을 선택, 집중화하고 핵심기술을 확보하여 경쟁력을 키우는 전략으로 매우 유효할 것으로 판단된다. 본 고에서는 Advanced TCA의 전망 및 기술적 특성에 대하여 알아본다.

An Optimal Design Strategy for a Thomson Coil Actuator

  • Lim, Dong-Kuk;Jung, Sang-Yong;Jung, Hyun-Kyo;Ro, Jong-Suk
    • Journal of Electrical Engineering and Technology
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    • v.12 no.1
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    • pp.182-188
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    • 2017
  • An arc eliminator allows a surge current bypass rapidly into the earth to facilitate the circuit breaking process and increase the stability of the load circuit. Thomson coil actuator (TCA) is a type of actuator that can function as an arc eliminator. The TCA has a simple structure and significantly rapid speed compared to the other types of actuators. In this paper, significant variables, which have a dominant effect on the performance of the TCA, are investigated in detail. Using these variables and an optimization algorithm, an optimal design strategy for the TCA is proposed in this research. The efficacy of the proposed optimal design strategy and the feasibility of the application of the designed TCA for a high power circuit breaker as the arc eliminators are validated through the experiment.

An Efficient TCA and MAC Frame Structure Design in ZigBee Networks (지그비 네트워크에서 효율적인 TCA 및 MAC 프레임 구조 설계)

  • Lee, Hyeop-Geon;Lee, Kyoung-Hwa;Won, Kwang-Ho;Shin, Yong-Tae
    • Proceedings of the Korean Information Science Society Conference
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    • 2010.06d
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    • pp.110-115
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    • 2010
  • 지그비에서는 Trust Center 역할을 정의하고 이를 통한 키 분배를 통해 안전한 통신을 한다. 그러나 현재 표준에서 사용하는 TCA 는 모든 트래픽이 Trust Center 로 집중됨으로써 부하 및 지연이 발생된다. 또한 표준에서 사용하는 보안 프로토콜은 서비스의 중요도와 상관없이 암 복호화를 수행한다. 본 논문에서는 지그비 네트워크에서 효율적인 TCA 및 MAC 프레임 구조 설계하였다. 제안하는 TCA 는 Trust Center 간의 키 분배를 통해 이전의 키 정보를 재사용한다. 또한 MAC 프레임에 추후 적용 가능할 서비스들을 위해 차등적 보안 레벨을 적용하여 에너지 효율성을 높이고, 통신 지연시간을 줄였다.

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Adding Effect of TCA-Precipitated Blood Powder to the Phenol Formaldehyde Resin for Plywood (합판용 페놀수지 접착제에 대한 TCA침전 혈액분말의 첨가효과)

  • Lee, Hwa-Hyoung;Lee, Jong-Shin;Jang, Sang-Sik
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.2
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    • pp.15-19
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    • 1996
  • To utilize the waste materials and develope wood adhesive from isolated bloods of slaughtered cow and pig and also to prevent water pollution, simple and rapid method of isolation and purification of plasma proteins from pig bloods with trichloroacetic acid(TCA) treatment was developed. Adding of TCA-precipitated blood powder to the phenol formaldehyde resin(PF) improved dry and wet strength of plywood and resulted in fast hot pressing times.

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Flux Regulation Patterns and Energy Audit of E. coli B/r and K-12

  • Lee, Jin-Won;Goel, Akshay;Ataai, Mohammad-M.;Domach, Michael-M.
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.258-267
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    • 2002
  • A flux determination methodology has been built which enables to develop constrained stoichiometric relationships and metabolic balances. The analysis differs from those developed for anaerobic growth conditions in that cell mass formation is a significant sink for carbon. When combined with experimental measurements, a determined system of equations results yielded tricarboxylic acid (TCA) cycle and glycolytic fluxes. The methodology was implemented to determine the fluxes of E. coli B/r and K12, and it was found that as the growth rate in a glucose minimal medium increased, the cells became increasing glycolytic and the TCA fluxes either leveled off or declined. The pattern identified for the TCA fluxes corresponded to ${\alpha}$-ketoglutarate dehydrogenase's induction-repression pattern, thereby suggesting that the induction-repression of the enzyme could result in significant flux changes. When the minimum flux solution was contrasted to the glycolytic and TCA fluxes determined, two observations were made. First, the minimum flux could provide the cell's biosynthetic ATP requirements. Second, at a high growth rate in a glucose medium, the excess glycolytic flux exceeded that of the TCA cycle, which appeared to more closely match the biosynthetic needs.

Analysis of cell survival genes in human gingival fibroblasts after sequential release of trichloroacetic acid and epidermal growth factor using the nano-controlled release system (나노방출제어시스템을 이용하여 trichloroacetic acid와 epidermal growth factor의 순차적 방출을 적용한 인간치은섬유아세포의 세포생존 관련 유전자 연구분석)

  • Cho, Joon Youn;Lee, Richard sungbok;Lee, Suk Won
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.3
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    • pp.145-157
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    • 2020
  • Purpose: This study was to determine the possible effects of trichloroacetic acid (TCA) and epidermal growth factor (EGF) through cell survival genes of the PI3K-AKT signaling pathway when applying an hydrophobically modified glycol chitosan (HGC)-based nanocontrolled release system to human gingival fibroblasts in oral soft tissue regeneration. Materials and Methods: An HGC-based nano-controlled release system was produced, followed by the loading of TCA and EGF. The group was divided into control (CON), TCA-loaded nano-controlled release system (EXP1), and the TCA- and EGF- individually loaded nano-controlled release system (EXP2). A total for 29 genes related to the PI3K-AKT signaling pathway were analyzed after 48h of culture in human gingival fibroblasts. Real-time PCR, 1- way ANOVA and multiple regression analysis were performed. Results: Cell survival genes were significantly upregulated in EXP1 and EXP2. From multiple regression analysis, ITGB1 was determined to be the most influential factor for AKT1 expression. Conclusion: The application of TCA and EGF through the HGC-based nano-controlled release system can up-regulate the cell survival pathway.