• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.85-85
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• 2001

본 연구는 돼지 난자의 체외 수정시 sucrose 첨가가 돼지 난자의 다정자 침입율에 미치는 영향을 조사하기 위하여 실시하였다. 돼지 난포란은 0.1% PVA, 3.05 mM D-glucose, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 0.5 $\mu\textrm{g}$/$m\ell$ FSH, 0.5 $\mu\textrm{g}$/$m\ell$ LH, 및 10 ng/$m\ell$ EGF가 첨가된 TCM199 배양액에서 42-44 시간 동안 배양하여 체외 성숙을 유도하였으며, 2 mM caffeine과 0.1% BSA가 첨가된 체외수정용 mTBM 배양액에 3% sucorse를 첨가하여 6시간동안 수정을 유도하였다. 수정된 난자의 다정자 침입율을 조사하기 위해 체외수정 후 12, 13 및 14 시간대에 각각 1% orcein으로 염색하여 전핵 형성율을 조사하였다. 돼지 난자의 체외수정시 3% sucorse를 첨가하였을 때, 전핵 형성율을 조사한 결과 대조구에서 미수정, 1, 2, 및 3 PN 이상의 전핵 형성율은 각각 57.6 7.6, 9.7, 및 25%로, 처리구 74.2, 7.6, 13.6, 및 4.5% 에 비교하여 다정자 침입율이 유의하게 높았다(p<0.05). 그리고 3% sucorse를 첨가한 체외수정용 배양액에서 수정을 유도한 후 12, 13, 및 14 시간대별로 나누어 전핵 형성율을 조사한 결과, 수정 후 시간이 경과되면서 다정자 침입율이 증가되는 것을 볼 수 있었다 그러나 대조구 60.7%에 비해서는 각각 32.5, 36.0, 및 33.3%로 현저히 낮은 것을 볼 수 있었다.(Table Omitted). 또한, 3% sucorse가 첨가된 배양액에서 수정된 수정란의 발달율을 조사한 결과, 배반포 생산율은 처리구 21.8%로 대조구 6.9%에 비해 유의하게 높은 것을 확인 하였다. 따라서, 돼지 난자의 체외수정시 3% sucorse첨가는 체외수정란 생산에서 문제가 되고 있는 다정자 침입율을 저하시키고 배발달율을 향상시킴으로서 수정란의 체외 대량생산 효율성은 물론 수정란 이식시 수태율을 증가시킬 수 있을 것으로 사료된다.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.97-104
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• 2008

Plasminogen activators (PAs) are serine protease that cleave plasminogen to form the active protease plasmin. PA/plasmin system playa role in mammalian fertilization and motility and acrosome reaction of sperm. The present study was undertaken to identify PAs in porcine gametes and investigate a possible role of plasminogen in in vitro fertilization in the pig. When boar spermatozoa were preincubated in a fertilization medium (mTBM) for 0, 2, 4 or 6 h, the activity of tPA-PAI ($110{\sim}117\;kDa$), tPA ($62{\sim}70\;kDa$), and uPA ($34{\sim}38\;kDa$) was observed in the sperm incubation medium and sperm sample. PA activities in the sperm incubation medium significantly (p<0.05) increased according to increasing incubation times, while PA activities in sperm significantly (p<0.05) decreased at the same times. In addition, the rate of acrosome reaction in spermatozoa increased by increasing culture times. When oocytes were separated from porcine cumulus-oocytes complexes at 0, 22 or 44 h of maturation culture, no PA activities were observed in cumulus free-oocyte just after aspiration from follicles. However, the activity of tPA-PAI ($108{\sim}113\;kDa$) and tPA ($75{\sim}83\;kDa$) was observed at 22 h of in vitro culture and significantly (p<0.05) increased as the duration of the culture increased. On the other hand, when porcine oocytes were activated by sperm penetration or calcium ionophore, plasminogen significantly (p<0.05) increased ZP dissolution time (sec) in activated oocytes by sperm penetration. These results suggest that supplementation of plasminogen to fertilization medium may playa positive role in the improvement of in vitro fertilization ability in the pig.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.1
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• pp.99-118
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• 2018

Recently, the limit state design method in the design of the structure is in global trend, but it is limited to a few structures in Korea. Since the introduction of the limit state design method has recently been attempted for tunnels, which are the main underground structures, it is surely necessary to understand the latest limit state design method. Therefore, based on the recently published AASHTO LRFD Road Tunnel Design and Construction Guide Specification (2017), structural load factors and load combinations were reviewed, and various factors which should be applied for the review of structures have been analyzed. In this study, utility tunnel section and subway tunnel sections used in Korea were analyzed by the limit state design method, and we have analyzed the direction of application of limit state design method through studying the tendency of member force by various influential factors such as ground conditions, load modifier and joint stiffness.

• Journal of the Korea Safety Management & Science
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• v.10 no.3
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• pp.89-98
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• 2008

A preventive maintenance model, caller FNBM($\alpha$, $\delta$, $\gamma$)model, is proposed to decide an optimal repair number under achieved availability requirements(r) along with taking two types of failures (repairable or irrepairable) into account. In this model, the current system is replaced by a new one in case when it doesn't meet the achieved availability requirement, even though it is repairable failure; Otherwise it is replaced in time of the first irrepairable failure. Assumed that the j-th failure is repairable with probability ${\alpha}_j$ minimal repairs are allowed for repairable failure between replacements. Expected cost rate for preventive maintenance model is developed using NHPP(Non-Homogeneous Poisson Process) in order to determine the optimal number $n^*$, also numerical examples are shown in order to explain the proposed model. Since the proposed FNBM($\alpha$, $\delta$, $\gamma$)model includes Park FNBM model(1979) and Nakagawa FNBM(p)model(1983) this proposed model is thought to be better than previous model, especially for weapon system which requires availability as primary parameter.

• Journal of the Korean GEO-environmental Society
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• v.4 no.3
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• pp.79-88
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• 2003

This paper presents a numerical study of the spatial behavior of a linear absorbing solute in a heterogeneous porous medium. The spatially correlated log-normal hydraulic conductivity field is generated in a given two-dimensional domain by using the geostatistical method (Turning Bands algorithm). The velocity vector field is calculated by applying the two-dimensional saturated groundwater flow equation to the Galerkin finite element method. The simulation of solute transport is carried out by using the random walk particle tracking model with CD(constant displacement) scheme in which the time interval is automatically adjusted. In this study, the spatial behavior of a solute is analyzed by the longitudinal center-of-mass displacement, longitudinal spatial spread moment and longitudinal plume skewness.

• Tunnel and Underground Space
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• v.23 no.5
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• pp.428-441
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• 2013

Nowadays, a roadheader as a mechanical excavator is in high demand, especially for mines under various conditions and tunnels where TBMs are inapplicable. However, the records of roadheaders in Korea are seldom reported. Moreover, the number of countries with their intrinsic design and manufacturing technologies of roadheaders is very limited. Therefore, this study aimed to analyse the excavation principles of roadheader as well as its key design parameters for its optimized selection and design. In addition, the database with 143 world-widely collected roadheader design data was built, and a few statistical correlations were derived from it. A schematic procedure for roadheader design based on the database was also proposed.

• Journal of the Korean Institute of Gas
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• v.23 no.6
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• pp.25-32
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• 2019

In many mass-consumption gas facilities, natural gas is not supplied through the pipeline of the gas corporation. LNG is supplied from the gas corporation through the tank lorry to be vaporized. In order to prevent human or property damage due to gas leakage at these facilities, a study was conducted to analyze the concentration of odorant injected at the initial and consumption points. An analysis was performed to confirm the change in odorant concentration according to the pipe position in the gas facility when a constant flow rate flowed. For this study the gas samples were taken with aluminium cylinders(4.5 L) which were created a vacuum at the pressure regulator in which the odorants was injected and the points using the gas. Odorant levels of the samples were analyzed by Gas chromatography(Main Body : Agilent 7890A, Detector : ANTEC 7090).We suggest that the small facilities using LNG need to make the management system by the types of facilities for maintaining the odorization system.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.1200-1201
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• 2015

고전압 전력설비 진단은 기기의 열화 상태를 측정하여 이상 사고를 미리 예측하여 방지하는 것을 목적으로 실시한다. 고전압 전력설비의 유지관리 방안은 일정 시간 경과후 보수하는 개념(TBM: Time Based Maintenance) 이후 설비의 상태를 진단하여 유지보수 방안을 결정하는 개념(CBM: Condition Based Maintenance)으로 진보해 감에 따라 전력설비의 상태진단 기술의 중요성은 증대될 전망이다. 고전압 전력설비의 절연진단은 직류시험(절연저항, PI)과 교류시험($tan{\delta}$, PD)이 실시되며 과거 진단 데이터의 추세분석을 통한 정확한 상태진단이 요구되고 있다. 고압 유도 전동기 절연진단 데이터 관리 전산화 모델은 고전압 전력기기(발전기, 변압기, 전동기, 케이블 등)의 절연진단 및 유지보수 이력에 관한 자료들을 저장, 조회 및 검색을 하기 위한 데이터베이스를 구축하고 구축된 데이터를 활용하여 과거 이력조회, 추이분석, 진단 데이터의 분석기법을 통한 전력기기의 상태평가로 합리적인 개 대체 의사결정을 지원한다. 또한, 유입식 변압기의 절연유 가스분석 알고리즘을 전산화 하여 10종 가연성 가스에 따른 Gas Pattern 평가로 고장 원인, 현상 및 조치 등에 대한 출력이 가능한 프로그램의 개발로 고전압 전력설비 진단기술과 IT기술의 융 복합 기술로서 고전압 전력설비의 유지관리 기술을 한 차원 더 진보시킬 것으로 판단된다.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.415-415
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• 2011

국제핵융합실험로(ITER)에 장착되는 한국형 헬륨 냉각 고체형 증식(Helium Cooled Solid Breeder : HCSB) 시험 블랑켓(Test Blanket Module : TBM)은 ITER 참여국 중 유일하게 중성자 반사 재료를 채택한 것이 특징이다. 중성자 반사재료로는 지름 1 mm 내외의 흑연 페블을 사용 할 예정이다. 흑연은 중성자 반사특성은 우수하지만 기계적 특성이 비교적 좋지 않다는 단점이있다. 뿐만 아니라, 산화나 화재 등에 대해서도 취약하기 때문에 흑연이 노출된 상태로 사용하는 것은 위험부담이 클 수밖에 없다. 따라서 흑연을 코팅해서 사용하기 위한 연구개발이 진행 중이며, 코팅 후보물질로는 저방사화 및 고경도의 특성을 갖는 SiC가 유력시 되고 있다. 흑연위에 SiC를 코팅하는 방법은 여러 가지가 있으며, 그 중에서 비교적 간단한 RF Sputtering, PECVD를 이용해서 SiC를 코팅하고 그 특성을 평가했다. RF Sputtering에서 흑연의 온도를 상온으로 두었을 때는 SiC가 결정으로 성장되지 않는 것을 확인할 수 있었으며, $900^{\circ}C$ 이상의 온도에서 열처리과정을 거친 후 결정이 형성되는 것을 확인할 수 있었다. 그리고 열처리 온도가 $1200^{\circ}C$ 부근에서는 SiC nano-wire가 형성되는 것을 확인할 수 있었다. PECVD의 경우 전구체 물질로 사용된 $SiH_4$와 $CH_4$의 비율에 따라서 SiC의 형성비율이 다른 것을 알 수 있었으며, 결정 상태는 성장시 기판온도에 크게 의존하는 것을 확인할 수 있었다. 최근에는 보다 효율적으로 SiC를 코팅하기 위하여 흑연페블을 spouting시키면서 코팅할 수 있는 CVD 장치를 설계-제작했으며, 전구체 물질로는 $SiH_4$, $Si(CH_3)_4$, $CH_3$ $SiCl_3$ 등이 사용될 예정이다.

• Geophysics and Geophysical Exploration
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• v.7 no.1
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• pp.62-69
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• 2004

The objective of this study is to estimate the distribution of a zone disturbed by excavation (EDZ) around tunnels that have been excavated at about 500 m depth in pre-Tertiary hard sedimentary rock. One of the most important tasks is to evaluate changes in the dynamic stability and permeability of the rock around the tunnels, by investigating the properties of the rock after the excavation. We performed resistivity and acoustic tomography using two boreholes, 5 m in length, drilled horizontally from the wall of a tunnel in pre-Tertiary hard conglomerate. By these methods, we detected a low-resistivity and low-velocity zone 1 m in thickness around the wall of the tunnel. The resulting profiles were verified by permeability and evaporation tests performed at the same boreholes. This anomalous zone matched a high-permeability zone caused by open fractures. Next, we performed resistivity monitoring along annular survey lines in a tunnel excavated in pre-Tertiary hard shale by a tunnel-boring machine (TBM). We detected anomalous zones in 2D resistivity profiles surrounding the tunnel. A low-resistivity zone 1 m in thickness was detected around the tunnel when one year had passed after the excavation. However, two years later, the resistivity around the tunnel had increased in a portion, about 30 cm in thickness, of this zone. To investigate this change, we studied the relationship between groundwater flow from the surroundings and evaporation from the wall around the tunnel. These features were verified by the relationship between the resistivity and porosity of rocks obtained by laboratory tests on core samples. Furthermore, the profiles matched well with highly permeable zones detected by permeability and evaporation tests at a horizontal borehole drilled near the survey line. We conclude that the anomalous zones in these profiles indicate the EDZ around the tunnel.