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Identification of Coccoidal Bacteria in Traditional Fermented Milk Products from Mongolia, and the Fermentation Properties of the Predominant Species, Streptococcus thermophilus

  • Ren, Yan;Liu, Wenjun;Zhang, Heping
    • Food Science of Animal Resources
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    • v.35 no.5
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    • pp.683-691
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    • 2015
  • The objective of this study was to identify the coccoidal bacteria present in 188 samples of fermented yaks’, mares’ and cows’ milk products collected from 12 different regions in Mongolia. Furthermore, we evaluated the fermentation properties of ten selected isolates of the predominant species, Streptococcus (S.) thermophiles, during the process of milk fermentation and subsequent storage of the resulting yoghurt at 4℃. Overall, 159 isolates were obtained from 188 samples using M17 agar. These isolates were presumed to be lactic acid bacteria based on their gram-positive and catalase-negative properties, and were identified to species level using 16S rRNA gene sequence analysis. These coccoid isolates were distributed in four genera and six species: Enterococcus (E.) durans, Enterococcus (E.) faecalis, Lactococcus (Lac.) subsp. lactis, Leuconostoc (Leuc.) lactis, Leuconostoc (Leuc.) mesenteroides. subsp. mesenteroides and S. thermophilus. Among these S. thermophilus was the most common species in most samples. From evaluation of the fermentation characteristics (viable counts, pH, titratable acidity [TA]) of ten selected S. thermophilus isolates we could identify four isolates (IMAU 20246, IMAU20764, IMAU20729 and IMAU20738) that were fast acid producers. IMAU20246 produced the highest concentrations of lactic acid and formic acid. These isolates have potential as starter cultures for yoghurt production.

Change in the Spatial Structure of Commercial Areas in Daegu (대구시 상업지역의 구조 변화)

  • Kim, Ta-Yeul;Jin, Won-Hyung
    • Journal of the Korean association of regional geographers
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    • v.14 no.4
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    • pp.367-381
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    • 2008
  • The purpose of this paper is to analyze the change in the distribution and hierarchical structure of commercial land use. Tools for analyzing the spatial structure are index of concentration, coefficient of localization and location quotient. This research is applied to Daegu, focusing on the period 1985-2003. During the 1985-2003 period, the growth rate of commercial land use volume amounted to 330%, compared with a population growth rate of 118%. The biggest component of commercial land use is the retail sector. As the lodging, medical, transportation-warehouse and entertainment sectors have the propensity for concentration and comprise the specialized commercial areas in the suburbs, the other sectors arc evenly distributed. Jung-gu has functioned as a downtown core in the hierarchical structure of commercial areas. So, in the structure of commercial land use, Daegu has continued to be a single nuclear structure. But, Dongdaegu Station influence area has evolved into the second order center since 2000. This is the sign of change in spatial structure from single-nuclear pattern to multi nuclear pattern.

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Development of Fecal Microbial Enzyme Mix for Mutagenicity Assay of Natural Products

  • Yeo, Hee-Kyung;Hyun, Yang-Jin;Jang, Se-Eun;Han, Myung-Joo;Lee, Yong-Sup;Kim, Dong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.6
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    • pp.838-848
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    • 2012
  • Orally administered herbal glycosides are metabolized to their hydrophobic compounds by intestinal microflora in the intestine of animals and human, not liver enzymes, and absorbed from the intestine to the blood. Of these metabolites, some, such as quercetin and kaempherol, are mutagenic. The fecal bacterial enzyme fraction (fecalase) of human or animals has been used for measuring the mutagenicity of dietary glycosides. However, the fecalase activity between individuals is significantly different and its preparation is laborious and odious. Therefore, we developed a fecal microbial enzyme mix (FM) usable in the Ames test to remediate the fluctuated reaction system activating natural glycosides to mutagens. We selected, cultured, and mixed 4 bacteria highly producing glycosidase activities based on a cell-free extract of feces (fecalase) from 100 healthy Korean volunteers. When the mutagenicities of rutin and methanol extract of the flos of Sophora japonica L. (SFME), of which the major constituent is rutin, towards Salmonella typhimurium strains TA 98, 100, 102, 1,535, and 1,537 were tested using FM and/or S9 mix, these agents were potently mutagenic. These mutagenicities using FM were not significantly different compared with those using Korean fecalase. SFME and rutin were potently mutagenic in the test when these were treated with fecalase or FM in the presence of S9 mix, followed by those treated with S9 mix alone and those with fecalase or FM. Freeze-dried FM was more stable in storage than fecalase. Based on these findings, FM could be usable instead of human fecalase in the Ames test.

Determination of Oxidation Stability of Perilla Oil by the Rancimat Method (랜시매트법에 의한 들기름의 산화안정성 측정)

  • Cha, Ga-Seong;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.22 no.1
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    • pp.61-65
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    • 1990
  • Comparison of induction times among the Rancimat Method, the Oven Test and the Weight Method were investigated, and the oxidation stability of perilla oil was determinated by the Rancimat Method. The induction times determinated by three methods using perilla oil show a good correlation. And the temperature dependence of the induction times of perilla oil was as follows. $t_p=e^{-0.0815T+9.183}$. The antioxidant effect of Tocopherols on the oxidation of perilla oil was not observed, and the antioxidant effect of the organic acids was increased in the order of Ascorbic acid>Ascorbyl stearate>Malic acid>Tartaric acid>Citric acid. The synergistic effect of Ascorbic acid (500 ppm)and Tocopherols was not observed below 200ppm of Tocopherols, but became larger with increasing Tocopherols level over 200ppm.

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The Effects of Personality Traits on Stress Level, Stressor, and Stress Coping Style : Focused on S High School Students (성격특성이 스트레스 수준, 발생요인, 대처방식에 미치는 영향 : S고등학교 학생들을 중심으로)

  • Jo, Eun-Hyeon;Lee, Dong-Hyung
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.40 no.3
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    • pp.107-115
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    • 2017
  • The purpose of this study is to find and compare the differences in stress level, stressor, and coping style of high school students according to their personality traits. And to identify the personality traits contributing to stress reduction. The results of the study can be used as basic data for reducing the stress of adolescents to improve their academic achievement and maintain mental and physical stability. The questionnaire survey was conducted for 10 days from June 7 to June 16, 2017 for S high school students in Chungbuk Province. The study employed four measuring instruments as Ego-gram, Ok-gram, stressor scale, and stress coping type scale. Individual personality traits were classified into ego-states and ok-states in TA (Transactional Analysis). Stressors are classified into 5 categories (school life, home life, human relationship, myself, environment) and stress coping styles are classified into 4 categories (problem-focused coping, social-support coping, feeling-focused coping, hope-thinking coping) used in the previous studies. The collected data were analyzed using T-test, ANOVA, and multiple regression analysis. As a result, the stress level of high school students showed significant differences according to sex, year, grade, sleeping time, personality traits, and coping styles. Personality traits that contributed to the stress increase were NP, AC, 'you positive', 'i negative' and personality trait that contributes to stress reduction is 'you negative.' Therefore, we can judge that individual personality traits have significant effects on stress level, stressor, and coping style and need to find the effective stress management method suitable for individual personality traits using counseling, repetition training, self-suggestion etc.

Host range, Life cycle and Natural enemies of Mulberry scale (Pseudaulacaspis pentagona) on Prunus mume in south Korea (매실나무에 기생하는 뽕나무깍지벌레 (Pseudaulacaspis pentagona)의 기주범위, 생활사 및 천적에 관한 조사)

  • 박종대;김규진
    • Korean journal of applied entomology
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    • v.29 no.2
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    • pp.104-112
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    • 1990
  • Studies were conducted to investigate hosts, life cycle, population dynamics and natural enemies of mulberry scale, Pseudaulacaspis pentagona Ta. & Tozz., from 1987 to 1988 in southern region of Korea. The host plants were 22 families and 74 species. Host plants belonged to genus Prunus in general were severely damaged due to mulberry scale. Mulberry scale was most likely to have three generations a year with first occurrence of larval stage from mid-May to late June, second occurrence from mid-July to mid-August and third occurrence from early September to late October including 3 peaks in early and mid-June, late July and mid-September. In case of each stage occurrence, eggs were peaked on 10th day, larvae on 25th day and adults on 7th day from beginning of occurrence, respectively. Natural enemies were observed as parasitoids of 3 species such as Aphytis diaspidis, Archenomus orientalis and Apterencyrtus mocrophagus and as predators of 3 species such as Chilocorus Kuwanae, Chilocorus rubidus and Hormonia axyridis. Shoot growth was affected by mulberry scale and Lepra to dwindle as much as half of normal growth.

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Effects of Indirect Moxibustion on Skeletal Muscles in Mouse Model of Skeletal Muscle Adiposity (간접구 시술이 골격근 Adiposity 유발 쥐의 근육조직에 미치는 영향)

  • Lee, Ki Su;Hong, Kwon Eui
    • Journal of Acupuncture Research
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    • v.31 no.1
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    • pp.7-21
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    • 2014
  • Objectives : To observe the regenerative effects of indirect moxibustion, a traditional Korean medical treatment on skeletal muscles using mouse model of skeletal muscle adiposity. Methods : Twenty seven ICR male mice were randomly assigned into Intact control(n=3), glycerol treatment together without moxibustion(n=12), and glycerol treatment together with moxibustion (n=12) groups. Mice of glycerol treatment groups were injected with 50 ${\mu}l$ DW(distilled water) containing 50 % of glycerol into the two tibialis anterior. After injection, moxibustion was applied at 'Shenshu'($BL_{23}$) and 'Zusanli'($ST_{36}$) acupoints three times per each session, every days for twelve days(total 12 treatments). Phospho-Erk1/2, Myostatin protein levels were analyzed by western blotting and immunofluo-rescence staining techniques for tissues of the tibialis anterior muscle. Smad, phospho-Smad were analyzed by immunofluorescence staining. Results : 1. Histological analysis of sections from injected TA muscles showed that glycerol induced rapidly muscle necrosis, with a maximum at day 3. 6 days and 9 days after injection, muscle was regenerating. 2. According to western blotting and immunofluorescence staining, phospho-Erk1/2 protein signals in glycerol treatment with moxibustion group were stronger compared to Intact and glycerol treatment without moxibustion group. 3. According to western blotting and immunofluorescence staining, myostatin protein signals in glycerol treatment without moxibustion group were stronger compared to Intact and glycerol treatment with moxibustion group. 4. According to immunofluorescence staining, Smad protein signals in glycerol treatment without moxibustion group were stronger compared to Intact and glycerol treatment with moxibustion group. 5. According to immunofluorescence staining, phospho-Smad protein signals in glycerol treatment without moxibustion group were stronger compared to Intact and glycerol treatment with moxibustion group. Conclusions : These results confirm that indirect moxibustion of 'Shenshu'($BL_{23}$) and 'Zusanli'($ST_{36}$) influences muscle regeneration in mouse models of skeletal muscle adiposity. Further discussion, and the establishment of moxibustion mechanism will prompt clinical application of moxibustion.

Piezoelectric and Dielectric Characteristics of (Na,K)$NbO_3$ Ceramics System According to Variations of Sintering Time (소결시간변화에 따른 (Na,K)$NbO_3$계 세라믹스의 유전 및 압전특성)

  • Kim, Do-Hyung;Yoo, Ju-Hyun;Jeong, Young-Ho;Hong, Jae-Il
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.48-48
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    • 2008
  • 현재 압전재료로써 사용되고 있는 PZT계 세라믹스는 우수한 압전 및 유전특성으로 초음파 센서, 압전변압기, 액츄에이터, 필터, 레조네이터와 같은 여러 응용분야에 널리 사용되어지고 있다. 그러나 납을 60%이상 포함하므로 환경오염에 의하여 그 사용에 대한 규제가 강화되고 있어 최근에는 납을 함유하지 않은 무연 환경친화형 압전세라믹스가 주목받고 있다. 현재 무연 조성 세라믹스에는 텅스텐-브론즈 형, 비스머스 레이어형, 페로브스카이트 형 등이 이으며, 그 중 페로보스카이트 혈을 제외하고는 보통 소성법 조제시 낮은 압전특성을 갖는다. 그러므로 압전특성을 증가시키기 위하여 Hot pressing, Hot forging, RTGG(Reactive Template Grain Growth), SPS(Spark Plasma Sintering), 그러나 이는 무연 세라믹스의 대량생산 과정에서 어려운 문제를 가지고 있고, 저가격 관점에서 볼 때 보통소성법이 보다 바람직하다. 그래서 보통소성법으로 NKN세라믹스의 소결성을 향상 시키고 비교적 높은 압전특성을 얻기 위해서 $LiSbO_3$, $LiNbO_3$, $LiTaO_3$, alkaline-earth(Mg, Ca, Sr, Ba) 등을 첨가한 논문들이 보고되고 있으며, 이러한 재료들의 kp는 대략 0.3-0.4를 나타내고 있다. 따라서, 본 연구에서는 소결온도를 낮추고자 $Ag_2O$를 소결조제로 사용하였고 유전 및 압전특성을 높이고자 소결시간 변화시켜 시편을 제작하여 NKN 세라믹스의 유전 및 압전특성을 조사하였다.

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Study on pathology of Alzheimer's disease, trends and future strategy for research (치매의 병리(病理), 연구동향(硏究動向)과 향후(向後) 연구전략(硏究戰略)에 대(對)한 고찰(考察))

  • Oh, Young-Sun;Kim, Sung-Hoon
    • Journal of Haehwa Medicine
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    • v.8 no.1
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    • pp.793-825
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    • 1999
  • For the development of drugs for alzheimer,s disease, the study was done to review the oriental pathology, clinical data, recent trends for research and strategy for future study. The results were as follows: 1. The medical term Chi-dsi implying alzheimer,s disease was referred for the first time in a medical book, Hwatasheneubijeon written by Hwa-Ta and its differentiation and treatment were studied more in Ming or Ching dynasties. Chi-dai can be differentated as weak(虛) syndrome and Shi(實) syndrome. This can be caused by deficiencies of renal Yin, renal Yang, cardiac Yin and hepatic blood, while that by deficiencies of pathological fluid(痰飮) and clotted blood(瘀血). 2. Dementia can be roughly classified as alzheimer's disease and multi-infarct disease. Its causes were known to be cholinergic transmitter, C-peptide, amyloid-${\beta}$, apolipoprotein, APP(amyloid precursor protein), TGF, MMP-9 and free radical. 3. In Korea experimental studies were chiefly done for the elimataion of C-peptide, amyloid-${\beta}$, apolipoprotein, APP for alzheimer's disease, for the development of drug inhibiting degerative change following CVA and loss of memory and also administrative measure was done by support of government. 4. Drugs of dimentia developed so far were Chi-Dai dan, extracts from aloe, mushroom, green tea, Ganoderma and also folic acid, vitamin C, DHEA and silk amino acid were reported to be effective in dimenta. 5. Future strategic research had better be done on dementia-inducing factors such as acetylcholine, C-peptide, amyloid-${\beta}$, apolipoprotein, APP, TGF, MMP-9 and free radical, development of animal model for dimentia, clinical study, epidemiology, nursing and administrative studies and also consortium for dimentia research should be formed so that repeated investment be avoided.

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Four New Records of Grenadiers (Macrouridae, Gadiformes, Teleostei) from Korea (한국산 민태과 Grenadiers (대구목) 어류 4 미기록종)

  • Kim, Seong-Yong;Iwamoto, Tomio;Yabe, Mamoru
    • Korean Journal of Ichthyology
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    • v.21 no.2
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    • pp.106-117
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    • 2009
  • A taxonomic study of the family Macrouridae was conducted using fish collections deposited in the Department of Biology, Chonbuk National University, Chonju, Korea and the East Sea Fisheries Research Institute, Korea. Among these collections were specimens of four species belonging to the genera Coryphaenoides and Coelorinchus: Coryphaenoides marginatus, C. microps, Coelorinchus (Abyssicola) macrochir, and C. formosanus. These specimens represent the first records of the species from Korean waters and from the East Sea (Japan Sea). Only four species (Coelorinchus longissimus, C. multispinulosus, C. japonicus, and C. parallelus) had previously been recorded from the East Sea. The Korean names "Keun-nun-gin-ga-si-min-te" (for Coryphaenoides marginatus), "Jak-eun-nun-gin-ga-si-min-te" (for C. microps), "Gin-pal-kko-ri-min-te" (for Coelorinchus macrochir), and "Ta-i-wan-kko-ri-min-te" (for Coelorinchus formosanus) are herein given to these species.