• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.18 no.1
• /
• pp.241-255
• /
• 2005

Objectives : In order to comprehend and utilize external therapy, we considered that we needed to research Wu Shi-Ji who was a medical specialist of external therapy. We looked at the special study on external therapy in his book, ${\ll}li-yao-pian-wen{\gg}$, especially ; in this part his generai approach to external therapy was well-detaiied. Methods : We translated and analyzed . Results : In , he gives a supplementary explanation about the insufficient attention paid to external therapy, 膏(gao) - (ti) 坐(zuo) (yu) 抹(mo) 纏法(chan fa), through San Jiao(三焦). And he insisted on his conception of external therapy: ointment(膏) and the moth of sneezing, sitting on herbs(坐), steaming or attaching herbs patients's navel, rubbing(抹) and binding(縛), based on ${\ll}nei-jing{\gg}$ and ${\ll}shang-han-lun{\gg}$. He put forward the general rules of external therapy using those methods. And through , he explained that the efficacy of his external therapy was basically the same as internal therapy and medicine, the only difference was in the method. In addition, this external therapy has some merits when compared with internal medicine. First, it's easier to diagnose a disease and treat patients compared to internal medicine. For instance, when a patient has a problem with his abdomen, we just have to diagnose a disease of the abdomen and treat using one method of external therapy on the abdomenal area. Second, external therapy has fewer side effects. And third, we can utilize the meridian theory when we use external therapy. Conclusions : Through , we basically understood his conception of external therapy. Although more research is needed, we suggest that we need to enlarge our usage of external therapy, not just confine ourselves to smaller areas of focus. His point of view suggests that external therapy is not merely an adjunct to internal therapy, but an interdependent course of study and action in its own right.

• Journal of Korean Academy of Nursing
• /
• v.15 no.3
• /
• pp.74-80
• /
• 1985

This study was attempted to identify the difference between body image of the patients with lower limb fracture and that of normal persons, and to examine if supportive group care could offer an apportunity for positive change in body image of the patient with lower limb fracture under the Quasi-experimental design. The subjects for this study were obtained by ta-king convenient sample of soldiers; the experimental group were 44 lower limb fractured patients hospitalized on the orthopedic Surgery unit in S Army General Hospital, while the control group were 44 normal enlisted men serving in the B Army regiment. Supportive group nursing care was given to the lower limb fractured patients who belong to the experimental group. Pre-and post-tests were administered to the experimental and the control group. The instruments to measure body image of the subjects were body Cathexis Scale developed by Scord and Jourard (1953) and Body Meaning Scale dove-loped by the reseacher. The reliability coefficients by Cronhach's u-test were .95 in body Cathexis Scale and .89 in Body Meaning Scale in this study. Data for this study were collected over a period 12 days from the 12th to the 24th of October, 1984 by the questionnaire. Data were analyzed by computer. Frequency, Percentage and x²-test were used to examine general chacteristics of the subjects. t-test was used to analyze the hypotheses. Analysis of variance was used to test difference in body image between groups classified by the general characteristics. Pearson Correlation Coefficient was used to identify the correlation between Body Cathexis Scale and Body Cathexis Scale and Body Meaning Scale. The results of this study were as follows: 1. No significant difference was found between the experimental and the control group on general characteristics of the subjects (p> .05). 2. Hypothesis I:“There will be a difference in body image between patiens with lower limb fracture and normal persons,”was supported(Body Cathexis t=6.91, p<.001, Body Meaning t=5.66, p< .001). 3. Hypothesis Ⅱ;“The will be a difference in body image of patients with lower limb fracture bet-ween after and before, supportive group nursing care was provided,”was supported (Body Cathexis t=5.90, p<.001, Body Meaning t=4.45, p <.001). 4. There was no significant difference in body image between groups classified by the general characteristics (p> .05). 5. The correlation between Body Cathexis Scale and Body Meaning Scale: It was reported that Body Cathexis Scale correlated with Body Meaning Scale in total subjects of the experimental and control group (r=.744, p<.001). That is, there was relatively high correlation between two scales. body Cathexis Scale correlated with Body Meaning Scale in the experimental group(r=.738, p <.001) and in the control group (r=.352, p <.001). That is, there was more than moderate correlation between two scales. In conclusion, it was found that there was a difference in body image between patients with lower limb fracture and normal persons, and supportive group nursing care offered an opportunity for positive change in body image of the patient with lower limb fracture.

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.11a
• /
• pp.97-97
• /
• 2002

Human eryhropoietin (EPO) is acidic glycoprotein hormone that plays key role in hematopoiesis by facilitating differentiation of erythrocyte and formation of hemoglobin (Hb) and is used for the treatment of anemia. Human EPO is consist of 166 amino acids which is modified by three N-glycosylations (24, 38, 83) and single O-glycosylation (126). N-glycosylation is reported to be related to the cellular secretion and activity of EPO. In this study, we examined effects of mutagenesis in glycosylation site of recombinat hEPO for the cellular secretion during production from cultured CHO cell. We produced rhEpo which was cloned by PCR from human liver cDNA (TaKaRa) in cultured CHO cell. Using supernatant of the culture, ELISA assay and western analysis were performed. To estimate biological activity, 20IU of rhuEpo was subcutaneously injected into four ICR mice. After 8 days, HCT level was increased average 13 per cent, RBC was increased ca. 2${\times}$10$\^$6//${\mu}\ell$. In disease model Rat (anemia c-kit, WSRC-WS/WS), HCT was increased ca. 12%, RBC was increased ca. 1.6${\times}$10$\^$6//${\mu}\ell$. These results suggests that rhEpo we produced has biological activity. To remove glycosylation site by substituting 24, 38, 83, and 126th asparagine (or serine) with glutamic acid, overlapping -extension site-directed mutagenesis was performed. To add novel glycosylation sites, 69, 105th leucine was mutated to asparagine. Mutant EPO construct was transfected into CHO cell. Supernatant of the cell culture was analyzed using ELISA assay with monoclonal anti-EPO antibody (Medac, Germany). Since, several reports for mutagenesis of glycosylation sites showed case-by-case results, we examined both transient expression and stable expression. Addition of novel glycosylation sites resulted no secretion while deletion mutants had little effect except some double deletion mutants (24/83 and 38/83) and triple mutant. We suggest that not single but combination of glycosyl group affect secretion of EPO.

• Environmental Analysis Health and Toxicology
• /
• v.5 no.3_4
• /
• pp.19-28
• /
• 1990

Nitroarenes are ubiquitous environmental pollutants displaying potent mutagenicity in bacteria and carcinogenicity in mammal. In this study, the concentration of nitroarenes in coarse and fine particles and mutagenicity of POC$\_$N/ fraction was investigated in suspended particulates at the Shinchon and Bulkwang area of Seoul. The suspended particulates were collected bimonthly by a high volume cascade impactor air sampler from July 1987 to May 1988. Extractable organic matter was obtained by ultrasonic extraction on diethly ether/cyclohexane (8/2, v/v). Neutral fraction was obtained by liquid-liquid extraction. Polar neutral organic compounds (POC$\_$N/) was fractionated by thin-layer chromatography. Finally, the concentrations of nitroarenes in POC$\_$N/ fraction were measured and determined by capillary gas chromatography. Direct and indirect mutagenicity of POC$\_$N/ fraction were measured using Salmonella typhimurium TA 98. The result were as follows: 1) Major nitroarenes at the Shinchon area was 1-nitropyrene and at the Bulkwang area it was 2,7-dinitro-9-fluorenone during the year. 2) Average concentration of total nitroarenes measured was 67.26 ng/m$^3$in fine particles which was 1,3 folds higher that in coarse particle (52.30 ng/m$^3$). 3) Annual pattern of nitroarenes concentrations revealed that concentration during heating season (Feb., Jan., Mar.) was 2.2 folds higher than that in non heating season (May, Jul., Sep.). Concentration of each season has 157.68 ng/m$^3$and 80.39 ng/m$^3$. 4) The mutagenic activity of POC$\_$N/ fraction from fine particles was higher compared to that of coarse particles and was increased when metabolically activated, with 59 mixture. Mutagenicities, Metabolically activated, were significantly different between Shinchon and Bulkwang area, 322.8 rev/250 $\mu\textrm{g}$/plate and 286.8 rev/250 $\mu\textrm{g}$/plate, respectively. 5) Annual pattern of mutagenicity of POC$\_$N/ fraction revealed that mutagenicity during the heating season was 1.7 folds higher at Shinchon area and 1.2 folds higher at Bulkwang than during the non heating season. The variable contents and levels of nitroarenes in suspended particulates may affect human health significantly. Further studies such as risk assessment should be conducted on the basis of these kind of studies.

• Analytical Science and Technology
• /
• v.24 no.3
• /
• pp.200-211
• /
• 2011

In this study, 13 compounds including four volatile fatty acids (VFA) and nine volatile organic compounds (VOC) were analyzed by cryogenic trapping-thermal desorption technique. In order to evaluate the analytical method for VFA, calibration experiments were performed using five different sorbent materials. When the calibration results are compared between different sorbents, sampling tube filled with Carbopack X showed the highest response factor (RF) for both VFA and VOC. To validate this new analytical method for VFA using cryogenic trapping-thermal desorption technique, this method was compared with alkali absorption method recommended by the odor prevention law of the Korea Ministry of Environment (KMOE). For this purpose, unknown samples were analyzed by two different methods, i.e., cryogenic trapping-thermal desorption (TD) and alkali absorption with solid phase microextraction (SPME). When the results of two different methods were compared, ratios of concentrations determined by the two analytical methods (TD/SPME) was found as 0.46 (valeric acid) ~ 0.71 (isovaleric acid). Therefore, additional study is required to properly establish and find stable analytical conditions for VFA analysis. Furthermore, comparison between two different methods should be made with more reliable calibration approaches.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.10 no.11
• /
• pp.3458-3464
• /
• 2009

This study was conducted to investigate the relationship between maximum oxygen uptake and its related factors including body measurement and vascular compliance. The subjects of our study were 43 males and 53 females aged 40 to 59 who were visited in military health center. The main results were that 1.the degree of obesity had significantly negative correlation to vascular compliance in male, and waist-hip ratio in female. 2. The degree of obesity, body mass index, waist-hip ratio and body fat percent had significantly negative correlation to maximum oxygen uptake in male, and waist-hip ratio & body fat percent in female. 3. Maximum heart rate had significantly positive correlation to maximum oxygen uptake in male, and vascular compliance & maximum heart rate in female. 4. In multiple regression analysis which maximum oxygen uptake was as dependent variable, body fat percent, vascular compliance and maximum heart rate were significant variables for both male and female. In conclusion, Maximum oxygen uptake of some rural people age 40 to 59 were affected by various factors such as body fat percent, vascular compliance, and maximum heart rate.

• Korean Journal of Applied Biomechanics
• /
• v.19 no.2
• /
• pp.347-357
• /
• 2009

The purpose of this study was to compare EMG and GRF during a free throw. Seven pairs of surface electrodes were attached to the right-hand side of the body to monitor the flexor carpi radialis (FC), extensor carpi radialis longus (EC), biceps brachii (BB), triceps brachii (TB), rectus femoris (RF), tibialis anterior (TA) and medial gastrocnemius (GM). GRF data from two force platform were collected during a free throw. The results showed that the muscle activities in the unskilled group must be highly activated prior to the moment of release. This means that a skilled participant can shoot a free throw more efficiently while producing less muscle activeness than an unskilled participant. The DCP of unskilled group in the medio-lateral direction were greater than the corresponding values in skilled group. This showed that the unskilled group were not able to shoot the free throws stably. Thus, when a teacher or instructor teaches students how to shoot free throw, it is considered that the teacher show the learner how to use not only the upper limbs but also lower limbs on the basis of the efficent connecting movement and the flexibility in a stable procedure.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2008.06a
• /
• pp.477-477
• /
• 2008

For more than three decades, the gate dielectrics in CMOS devices are $SiO_2$ because of its blocking properties of current in insulated gate FET channels. As the dimensions of feature size have been scaled down (width and the thickness is reduced down to 50 urn and 2 urn or less), gate leakage current is increased and reliability of $SiO_2$ is reduced. Many metal oxides such as $TiO_2$, $Ta_2O_4$, $SrTiO_3$, $Al_2O_3$, $HfO_2$ and $ZrO_2$ have been challenged for memory devices. These materials posses relatively high dielectric constant, but $HfO_2$ and $Al_2O_3$ did not provide sufficient advantages over $SiO_2$ or $Si_3N_4$ because of reaction with Si substrate. Recently, $HfO_2$ have been attracted attention because Hf forms the most stable oxide with the highest heat of formation. In addition, Hf can reduce the native oxide layer by creating $HfO_2$. However, new gate oxide candidates must satisfy a standard CMOS process. In order to fabricate high density memories with small feature size, the plasma etch process should be developed by well understanding and optimizing plasma behaviors. Therefore, it is necessary that the etch behavior of $HfO_2$ and plasma parameters are systematically investigated as functions of process parameters including gas mixing ratio, rf power, pressure and temperature to determine the mechanism of plasma induced damage. However, there is few studies on the the etch mechanism and the surface reactions in $BCl_3$ based plasma to etch $HfO_2$ thin films. In this work, the samples of $HfO_2$ were prepared on Si wafer with using atomic layer deposition. In our previous work, the maximum etch rate of $BCl_3$/Ar were obtained 20% $BCl_3$/ 80% Ar. Over 20% $BCl_3$ addition, the etch rate of $HfO_2$ decreased. The etching rate of $HfO_2$ and selectivity of $HfO_2$ to Si were investigated with using in inductively coupled plasma etching system (ICP) and $BCl_3/Cl_2$/Ar plasma. The change of volume densities of radical and atoms were monitored with using optical emission spectroscopy analysis (OES). The variations of components of etched surfaces for $HfO_2$ was investigated with using x-ray photo electron spectroscopy (XPS). In order to investigate the accumulation of etch by products during etch process, the exposed surface of $HfO_2$ in $BCl_3/Cl_2$/Ar plasma was compared with surface of as-doped $HfO_2$ and all the surfaces of samples were examined with field emission scanning electron microscopy and atomic force microscope (AFM).

• Economic and Environmental Geology
• /
• v.50 no.3
• /
• pp.181-193
• /
• 2017

Mn-Fe phosphate mineral complexes included within the pegmatite are observed at Jurassic Cheolwon two-mica granite in Gyeonggi Massif, South Korea. The genetic evolution between the Cheolwon two-mica granite and pegmatite, and various trend of Mn-Fe phosphate minerals is made by later magmatic, hydrothermal, and weathering process based on mineralogical, geochemical analysis. The Cheolwon two-mica granite is identified as S-type granite, considering its chemical composition (metaluminous ~ peraluminous), post-collisional environment, low magnetic susceptibility, and existence of biotite and muscovite. The K-Ar age (ca. 153 Ma) of pegmatite is well coincident with age of the Cheolwon two-mica granite ($151{\pm}4Ma$). It indicates that these two rocks are originated from the same magma. Pegmatite indicates the LCT geochemical signature, and was classified as muscovite-rare element class / Li subclass / beryl type / beryl-columbite-phosphate subtype pegmatite. The triplite $\{(Fe^{2+}{_{0.4}},Mn_{1.6})(PO_4)(F_{0.9})\}$ is dominant phosphates in later magmatic stage which partly altered to leucophosphite $\{KFe^{3+}{_2}(PO_4)_2OH{\cdot}2H_2O\}$ and jahnsite $\{(Fe^{3+}{_{0.7}},Mn_{2.3})(PO_4)_2OH{\cdot}4H_2O\}$ by hydrothermal alteration. In particular, near fractures, the triplite has been separatelty replaced by the phosphosiderite ($Fe^{3+}PO_4{\cdot}2H_2O$) and Mn-oxide minerals during weathering stage.

• The Korean Journal of Mycology
• /
• v.38 no.1
• /
• pp.34-39
• /
• 2010

We have attempted to verify 30 strains of Hypsizigus marmoreus from various mushroom stocks in Korea using random amplified polymorphic DNA (RAPD) methodology. Chromosomal DNAs of them were extracted and subjected to PCR analyses with 3 random primers. Each PCR produced approximately 30 distinct PCR bands with the size from 200 bp to 3000 bp. A dendrogram was acquired using the unweighted pair-group method with arithmetic average (UPGMA) clustering methodology on the basis of the DNA band pattern. The analysis revealed that 30 strains of H. marmoreus were clustered into two distinct clusters. Cluster 1 contained 3 subgroups while the cluster 2 consisted of rather diverse strains. Interestingly, Hm3-10, a wild strain collected from Deog-Yu mountain, was not included in either clusters, indicative of uniqueness of this strain. We nextly attempted to develop strain-specific DNA markers to verify a specific strain. A unique band in the RAPD gel lane of Hm0-4 was extracted and its sequence was determined. PCR with a primer set from the determined sequence revealed that the primer set gave a 250 bp DNA band only for Hm0-4, indicating that this approach works well for the strain-specific identification of H. marmoreus.