• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.3191-3196
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• 2013

Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a nonsmall cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with $IC_{50}$ values of 16, 17, and 17 ${\mu}g/ml$, respectively. Isolate f6l, however, showed no cytotoxicity towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical investigation using DAPI staining and biochemical investigation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method used to detect DNA fragmentation-together with caspase assay, demonstrated apoptotic cell death. Spectral characterization of isolate f6l revealed that it contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile and its mode of action, f6l might represent a new promising compound with potential for development as an anticancer drug with low or no toxicity to non-cancer cells used in this study.

• Clinical and Experimental Pediatrics
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• v.48 no.6
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• pp.634-639
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• 2005

Purpose : An understanding of the immunological process is required if primary prevention of atopic diseases is to be developed in early childhood. But, it is too hard to distinguish atopy from nonatopy under the age of two clinically, because the expression of phenotype and cytokines is vague in early childhood. We evaluated DNA methylation changes at Th2 interleukin-4 gene in peripheral blood from atopic children. Methods : We selected 15 allergic children(mild : eight, moderate to severe : seven) and seven normal controls by using family allergy scores and clinical histories. We measured Total IgE and Der f II specific IgE levels and cultured peripheral blood mononuclear cells with Der f II stimulation and extracted DNA from Der f II specific T cells. We examined the change of CpG methylation in DNA from atopic and nonatopic children. Results : In T cells from normal children, IL-4 DNA were predominantly methylated; otherwise, CpG demethylation occurred in Der f II specific T cells from allergic children. Conclusion : IL-4 DNA methylation changes occurred in T genes from allergic children and DNA methylation assay in early childhood.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.109-119
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• 2020

The purpose of this study was to provide basic data for improving health promotion behavior by identifying the effects of life stress and coping with stress among nursing college students on health promotion behavior. The data was collected from 352 nursing students in 6 colleges in B and K city, and the data was analyzed with SPSS/Win 23.0. Factors influencing health promotion behavior were school life satisfaction (F=9.18, p<.001), happiness score (F=20.64, p<.001), health status (F=12.40, p<.001), eating breakfast (t=-3.31, p=.001), unbalanced diet (t=-4.20, p<.001), time spent sleeping (F=2.64, p=.049), and exercise (t=5.20, p<.001). In the correlations, health promotion behavior was negatively correlated with life stress (r=-.289, p<.001), but it was positively correlated with coping with stress (r=.539, p<.001). The factors influencing health promotion behavior were stress coping (β=.439, p<.001), happiness score (β=.251, p<.001), exercise (β=.172, p<.001), unbalanced diet (β=.125, p=.002), life stress (β=-.121, p=.005), and eating breakfast (β=.083, p=.046). The above variables explained 46.0% for the health promotion behavior. Thus, in order to improve the health promotion behavior of nursing college students, integrated nursing interventions need to be developed through improving coping with stress, creating a sense of happiness, reducing life stress, and maintaining exercise and good eating habits.

• Journal of Biomedical Engineering Research
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• v.7 no.2
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• pp.117-118
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• 1986

It is well known that nonlinear propagation characteristics of the wave in the tissue may give very useful information for the medical diagnoisis. In this paper, a new method to detect nonlinear propagation characteristics of the internal vibration in the tissue for the low frequency mechanical vibration by using bispectral analysis is proposed. In the method, low frequency vibration of f0( = 100Hz) is applied on the surface of the object, and the waveform of the internal vibration x (t) is measured from Doppler frequency modulation of silmultaneously transmitted probing ultrasonic waves. Then, the bispectra of the signal x (t) at the frequencies (f0, f0) and (f0, 2f0) are calculated to estimate the nonlinear propagation characteristics as their magnitude ratio, w here since bispectrum is free from the gaussian additive noise we can get the value with high S/N. Basic experimental system is constructed by using 3.0 MHz probing ultrasonic waves and the several experiments are carried out for some phantoms. Results show the superiority of the proposed method to the conventional method using power spectrum and also its usefulness for the tissue characterization.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.3 no.2
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• pp.247-258
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• 1996

This study explored the variables to influence the stress of the family caregivers providing care for the hospitalized elderly The subjects for this study were 104 family caregivers selected from the caregivers in the in patient departments of two major medical centers in Jeonju city. Data were collected from December 18th to 27th, 1995 using a Liker Scale Questionaire and interview. Data analyses included percentage, mean, standard deviation, t and F-test. Results were following : 1. The mean of this sample is 21.48, and the standard deviation is ${\pm}5.64$ in the perceived stress. The major variables to influence on the stress of caregivere for the hospitalized elderly were the stay (16-24hrs/day) in hospital(F=7.99, p=0.006), no other helpers(t=-2.56, P=.012), percieving severely on illness(F=3.90, P=.0232) perceiving moderately on physical health status(F=6.20, P=.0029). 2. Regarding the perceived social support of the family caregivers, the higher support the group perceived the lower stress(F=3.54, P=.0326).

• Journal of the Korean Applied Science and Technology
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• v.35 no.1
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• pp.131-140
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• 2018

This study is a descriptive research study for analyzing the relevance among stress, anxiety, and ego-resilience in nursing college students before clinical practice. The survey was conducted targeting the second-year students in a K college who were anticipating their first clinical practice. The survey result showed that female students showed higher stress levels than male students (t=-5.51, p<.001) before clinical practice. Female students also showed higher anxiety levels than male students (t=-3.30, p=.001) before clinical practice. The students showed higher anxiety levels before clinical practice as they felt more strongly that they were in bad health (F=3.27, p=.041), as their personality was less outgoing (t=-3.32, p=.001), and as they had a lower satisfaction level in their major (F=7.25, p=.001). The students showed higher ego-resilience levels before clinical practice as they felt more strongly that they were in good health (F=3.74, p=.026) and as their personality was more outgoing (F=3.19, p=.002). The stress and anxiety levels before clinical practice also had a positive correlation (r=.211, p=.010) and the ego-resilience and anxiety before clinical practice had a negative correlation (r=-.313, p<.001). Based on the finding, it is necessary to develop the competence improvement program that can reduce the anxiety of nursing college students before clinical practice in order to improve their ego-resilience.

• International Journal of Reliability and Applications
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• v.12 no.1
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• pp.15-39
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• 2011

This paper deals with the reliability analysis of a complex system with three possibilities at the time of repair. The considered system consists of two subsystems A and Bin series configuration (1-out-of-2: F). Subsystem A has n units which are connected in series whereas subsystem B consists of n units in parallel configuration. The configuration of subsystem A is of 1-out-of-n: F whereas subsystem B is of k-out-of-n: D and k+1-out-of-n: F nature. System has three states: Good, degraded and failed. Supplementary variable technique has been used for mathematical formulation of the model. Laplace transform is being utilized to solve the mathematical equation. Reliability, Availability, M.T.T.F., Busy Period and Cost effectiveness of the system have been computed. The repairs from state $S_7$ to $S_0$, $S_8$ to $S_0$, $S_9$ to $S_0$ and $S_{11}$ to $S_0$ have two types namely exponential and general. Joint probability distribution of repair rate from $S_7$ to $S_0$, $S_8$ to $S_0$, $S_9$ to $S_0$ and $S_{11}$ to $S_0$ is computed by Gumbel-Hougaard family of copula. Some particular cases of the system have also been derived to see the practical importance of the model.

• Journal of Korean Academy of Nursing
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• v.28 no.4
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• pp.908-919
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• 1998

The purpose of this study was to get some basic data for health care for working women, especially or hospital nurses. The number of subjects was 796 nurses from one general hospital and four private educational hospitals. The data were collected from February to April, 1988 using the questionnaire "Symptom Table on Fatigue Perception" designed by the Research Committee of Industrial Fatigue in Hygienic Association of Japan Industry. The collected data were analyzed using means and standard deviations for the subjective symptoms of fatigue and each item as an independent variable was analyzed by T-test and ANOVA test. The results are as follows 1) The mean score for degree of fatigue was 1.89 and the degree of fatigue for physical symptoms had highest score of 2.04, the next was psychological symptoms at 1.89, and neuro -sensory symptoms were the lowest at 1.74. Among the fatigue symptoms, the item scored most frequently was "Legs feel heavy" with a mean score of 2.40 and the least frequent item was "My hand and foot trembled" with a mean score of 1.40. 2) With the respect to the general characteristics of subjects, there were statistically significant difference according to age (F=17.039, p=.000), state of marriage(t=5.381, p=.000) presence of children(t=5.134, p=.000), clinical experience (F=16.663, p= .000), present position(F=18.204, p=.000), working place(F=12.598, p=.000), duty time(F=9.068, p=.000), monthly wages(F=7.361, p=.000). satisfaction about the pay and treatment at work(t=-5.511, p=.000), relation the doctors(t=-4.593, p=.000) the doctors(t=-4.593, p=.000)

• Journal of Nutrition and Health
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• v.5 no.2
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• pp.83-89
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• 1972

A survey on the nutritional state was conducted from June 11 to Nov. 30, 1971, on 673 women workers $(16{\sim}25\;yrs)$ Jorking in a factory of food industry. Total body fat content was measured by skin-fold thickness (S. F. T.) with caliper at three region of the body. And it was calculated by regression equation, with the following conclusions were abtained. 1. The mean with standard deviation body height and weight of the Korean women workers were $155.0{\pm}4.21\;cm$ and $50.1{\pm}5.31\;kg$; respectively. 2. Their mean skin-fold thickness with their standard deviation triceps region of upper arm, 14.3{\pm}3.42$; Subscapular region, $18.0{\pm}5.57mm$; Abdominal region, $19.3{\pm}4.87mm$ and total aveager, 17.2{\pm}3.91mm$. 3. The body fat contents according to body fat weight regression equation were;fat weight. 10.7 kg; fat-free weight, 39.4kg; fat weight/ fat-free weight, 27.5%; fat-free mass/ total body mass 77.6%, and fat weight/ total body mass,21.6%. 4. Since the sknin-fold thickness between the triceps and upper abdominal region and between the former and Subscapular region were correlated with the coefficients of 0.838 and 0.615 respectively. and the following regression equations were established. a) S. F. T. of Subscapular region $(mm)=0.929{\times}$S. F. T. of triceps region (mm)+4.707. b) S. F. T. of Abdominal $region(mm)=1.24{\times}$S. F. T. of triceps region(mm)+1.50. Nutritional survey on the women workers in a fact ory of food industry, performed as above by the S. F. T. method, elucidated the fact that their nutritional state is as good as the control healthy female, and that their energy uptake may well be said to be satisfactory.

• The Journal of Korean Society for School & Community Health Education
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• v.14 no.1
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• pp.13-24
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• 2013

Background & Objectives: The aim of the study was to investigate characteristics and relationship among University students' attitude to reproductive health, value of marriage and children Methods: With a descriptive survey design, a self-report study was conducted and collected 470 responses from university students in Chungcheongnam-Do. Descriptive statistics, t-test, ANOVA, and Pearson's correlation coefficients were used to analyze data. Results: It was found that there were significantly different in attitude to reproductive health according to the following variables: age(F=2.91, p<.05), college(F=2.49, p<.05), sexual experience(t=7.54, p<.001). value of marriage was significantly different according to gender(t=-7.05, p<.001), college(F=4.42, p<.05), plan to marriage(t=-5.58, p<.001) and plan to having children(t=-4.30, p<.001). Value of children was significantly different according to gender(t=4.52, p<.001), plan to marriage(t=-4.30, p<.001) and plan to having children(t=6.33, p<.001). Attitude to reproductive health was significantly correlated with value of marriage(t=6.33, p<.001), value of marriage was significantly correlated with value of child(r=.224, p<.01). Conclusion: These results suggest that education for improving attitude to reproductive health, value of marriage and child are necessary to overcome low fertility. In addition, it is needed to further research and at the national level policy.