• Title/Summary/Keyword: T2r108

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Isolation and Identification of Three Newly Reported Ascomycete Fungal Species Isolated from Soil in Korea

  • Mohammad Hamizan Azmi;Seong-Keun Lim;Seok-Jin Park;Min-Gyeong Song;Jun-Soo Cha;Seung-Yeol Lee;Hee-Young Jung
    • The Korean Journal of Mycology
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    • v.52 no.2
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    • pp.97-108
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    • 2024
  • In this study, three fungal isolates belonging to the phylum Ascomycota under classes Leotiomycetes, Eurotiomycetes, and Sordariomycetes were isolated from soil in Korea. These species were designated as KNUF-22-003, KNUF-22-005, and KNUF-20-NI016, respectively, and identified based on their phylogenetic relationships and morphological characteristics. The isolates were confirmed through molecular phylogenetic analyses of their internal transcribed spacer (ITS) region, 28S rDNA large subunit (LSU), and actin (ACT1 ) gene sequences. Cultural and morphological characteristics of strains KNUF-22-003, KNUF-22-005, and KNUF-20-NI016 were matched with Chaetomella oblonga CBS110.78T, Oidiodendron chlamydosporicum CBS403.69T, and Sarocladium subulatum CBS217.35T, respectively. To the best of our knowledge, this is the first report on C. oblonga, O. chlamydosporicum, and S. subulatum in Korea.

The Dietary Effects of Fermented Chlorella vulgaris (CBT®) on Production Performance, Liver Lipids and Intestinal Microflora in Laying Hens

  • Zheng, L.;Oh, S.T.;Jeon, J.Y.;Moon, B.H.;Kwon, H.S.;Lim, S.U.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.261-266
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    • 2012
  • Fermented Chlorella vulgaris CBT$^{(R)}$ was evaluated for its effects on egg production, egg quality, liver lipids and intestinal microflora in laying hens. One hundred and eight Hy-line Brown layers (n = 108), 80 wk of age, were fed a basal diet supplemented with CBT$^{(R)}$ at the level of 0, 1,000 or 2,000 mg/kg, respectively for 42 d. Egg production was measured daily and egg quality was measured every two weeks. Five eggs from each replicate were collected randomly to determine egg quality. Egg production increased linearly with increasing levels of CBT$^{(R)}$ supplementation (p<0.05), although there was no significant effect of treatment on feed intake. Egg yolk color (p<0.001) and Haugh unit (p<0.01) improved linearly with increasing dietary CBT$^{(R)}$. Hepatic triacylglycerol level was linearly decreased with increasing dietary CBT$^{(R)}$ (p<0.05). The supplemental CBT$^{(R)}$ resulted in linear (p<0.001) and quadratic (p<0.01) response in population of cecal lactic acid bacteria. In conclusion, fermented Chlorella vulgaris supplemented to laying hen diets improved egg production, egg yolk color, Haugh unit and positively affected the contents of hepatic triacylglycerol and the profiles of cecal microflora.

Differences in Cognitive and Psychological Characteristics of Psychiatric Patients with Military Service Issues versus General Psychiatric Outpatients (군 복무 적합성 평가를 위해 정신건강의학과에 내원한 환자군과 일반 외래 환자군의 인지적 및 심리적 특성의 차이)

  • Shim, Seungyun;Choi, Junho;Kim, Eunkyeong
    • Korean Journal of Psychosomatic Medicine
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    • v.28 no.2
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    • pp.143-154
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    • 2020
  • Objectives : The purpose of this study was to examine cognitive and psychological characteristics of patients with military service suitability issues compared to the general psychiatric outpatients. Methods : 108 patients who visited psychiatric clinic center due to military service suitability issues and 80 general psychiatric patients were recruited from the Department of Psychiatry of university hospital. ANCOVA and chi-equare test were used to examine differences between two groups. Furthermore, we utilized paired t-test to compare the scrore within military group depending on when they performed the psychological assessment. Results : There were no significant differences between military group and general outpatient group in WAIS-IV scores. However, military group scored remarkably higher than control group on validity scales, F-r and Fp-r whereas they scored lower on validity scale, K-r. Furthermore, military group showed significantly higher on BDI and MMPI-2-RF, EID, RCd, RC2, RC3, COG, HLP, SFD, NFC, STW, SAVE, SHY, DSF, NEGE-r, INTR-r. As a result of comparison within the military group following the periods of assessment, military group did not show the significant differences on the overall scales of MMPI-2-RF. Conclusions : The present study showed that military group tends to report their psychological distress more exaggeratedly. In addition, they had significantly elevated not only emotional distress such as depression and anxiety but interpersonal problem. The implications and limitations were discussed along with some suggestions for the future studies.

A Study of the Relationship Among Health Promoting Behaviors, Climacteric Symptoms and Depression of Middle-Aged Women (중년여성의 건강증진행위와 갱년기 증상, 우울과의 관계 연구)

  • 유은광;김명희;김태경
    • Journal of Korean Academy of Nursing
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    • v.29 no.2
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    • pp.225-237
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    • 1999
  • The purpose of this study was to analyze the relationship among the health promoting behaviors, self-reported climacteric symptoms and depression on a cross-sectional survey desist The subjects were 108 middle-aged women who were nonhystrectomized and ranged in age from 40 to 60. They were selected in Seoul and Kyoung-ki province. Korea. Data were collected from Oct. 25 to Nov. 10, 1997 by a structured Questionnaire. The instrument used for this study was the revised Health Promotion Lifestyle Profile(HPLP) developed by Walker, Sechrist and Pender, revised Climacteric Symptoms Scale developed by Chi, Sung Ai, and the Beck's Depression Inventory(BID). The data were analyzed by the SPSS /PC$^{+}$ program using t-test, ANOVA and Scheffe test as a post hoc and Pearson Correlation Coefficient. The results of the study were as follows ; 1. The mean score of health promoting behaviors was low(2.42$\pm$0.35). There were statistically significant difference in the score of health promoting behaviors according to the educational background. family income, marital satisfaction, and whether or not taking a restoraitve food(t =-2.07, F=2.60~7.57, p<0.05). 2. The mean score of self-reported climacteric symptoms was 1.69 ; 99% of middle-aged women had symptoms. There were statistically significant difference in the score of middle-aged women's self-reported climacteric symptoms according to the age, number of children, educational background, occupation, family income, marital satisfaction, whether or not receiving hormone replacement therapy(HRT) or consultation experience with a professional, and perceived health status(t=-2.04~3.69. F=2.87~11.63, p<0.05). 3. The mean score of depression was 10.84. There were statistically significant differences in the score of the depression according to the age, number of children, educational background, occupation, marital satisfaction, whether or not receiving menopausal treatment or consultation by a professional, and perceived health status(t =-2.25~3.00, F=3.50~9.24, p<0.05). 4. Women's degree of health promoting behaviors was a negative correlation with the degree of climacteric symptoms(r=-0.19, p=0.03) and the degree of depression(r=-0.23, p=0.01). The degree of climacteric stmptoms was a positive correlation with the degree of depression(r=0.64 p=0.01). In conclusion. health promoting behavior should be considered when developing nursing strategies for middle-aged women. especially when dealing with climacteric symptoms and depression.

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Effect of Silicon Infiltration on the Mechanical Properties of 2D Cross-ply Carbon-Carbon Composites

  • Dhakate, S.R.;Aoki, T.;Ogasawara, T.
    • Carbon letters
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    • v.5 no.3
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    • pp.108-112
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    • 2004
  • Effect of silicon infiltration on the bend and tensile strength of 2D cross-ply carbon-carbon composites are studied. It is observed that bend strength higher than tensile strength in both types of composite is due to the different mode of fracture and loading direction. After silicon infiltrations bend and tensile strength suddenly decreases of carbon-carbon composites. This is due to the fact that, after silicon infiltration, silicon in the immediate vicinity of carbon forms the strong bond between carbon and silicon by formation silicon carbide and un-reacted silicon as free silicon. Therefore, these composites consist of three components carbon, silicon carbide and silicon. Due to mismatch between these three components secondary cracks developed and these cracks propagate from $90^{\circ}$ oriented plies to $0^{\circ}$ oriented plies by damaging the fibers (i.e., in-situ fiber damages). Hence, secondary cracks and in-situ fiber damages are responsible for degradation of mechanical properties of carbon-carbon composites after silicon infiltration which is revealed by microstructure investigation study by scanning electron microscope.

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Draft Genome of Toxocara canis, a Pathogen Responsible for Visceral Larva Migrans

  • Kong, Jinhwa;Won, Jungim;Yoon, Jeehee;Lee, UnJoo;Kim, Jong-Il;Huh, Sun
    • Parasites, Hosts and Diseases
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    • v.54 no.6
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    • pp.751-758
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    • 2016
  • This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

Conversion of Ginsenoside Rb1 and Taxonomical Characterization of Stenotrophomonas sp. 4KR4 from Ginseng Rhizosphere Soil (인삼 근권 토양에서 분리한 Stenotrophomonas sp. 4KR4의 Ginsenoside Rb1 전환능 및 분류학적 특성)

  • Jeon, In-Hwa;Cho, Geon-Yeong;Han, Song-Ih;Yoo, Sun Kyun;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.49 no.4
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    • pp.369-376
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    • 2013
  • We isolated the ${\beta}$-glucosidase producing bacteria (BGB) in ginseng root system (rhizosphere soil, rhizoplane, inside of root). Phylogenetic analysis of the 28 BGB based on the 16S rRNA gene sequences, BGB from rhizosphere soil belong to genus Stenotrophomonas (3 strains), Bacillus (1 strain), and Pseudoxanthomonas (1 strain). BGB isolates from rhizoplane were Stenotrophomonas (16 strains), Streptomyces (1 strain) and Microbacterium (1 strain). BGB from inside of root were categorized into Stenotrophomonas (3 strains) and Lysobacter (2 strains). Especially, Stenotrophomonas comprised the largest portion (approximately 90%) of total isolates and Stenotrophomonas was a dominant group of the ${\beta}$-glucosidase producing bacteria. We selected strain 4KR4, which had high ${\beta}$-glucosidase activity (108.17 unit), could transform ginsenoside Rb1 into Rd, Rg3, and Rh2 ginsenosides. In determining its relationship on the basis of 16S rRNA sequence, 4KR4 strain was most closely related to Stenotrophomonas rhizophila e-$p10^T$ (AJ293463) (99.62%). Therefore, on the basis of these polyphasic taxonomic evidence, the ginsenoside Rb1 converting bacteria 4KR4 was identified as Stenotrophomonas sp. 4KR4 (=KACC 17635).

Global Small Solutions of the Cauchy Problem for Nonisotropic Schrödinger Equations

  • Zhao, Xiangqing;Cui, Shangbin
    • Kyungpook Mathematical Journal
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    • v.48 no.1
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    • pp.101-108
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    • 2008
  • In this paper we study the existence of global small solutions of the Cauchy problem for the non-isotropically perturbed nonlinear Schr$\"{o}$dinger equation: $iu_t\;+\;{\Delta}u\;+\;{\mid}u{\mid}^{\alpha}u\;+\;a{\Sigma}_i^d\;u_{x_ix_ix_ix_i}$ = 0, where a is real constant, 1 $\leq$ d < n is a integer is a positive constant, and x = $(x_1,x_2,\cdots,x_n)\;\in\;R^n$. For some admissible ${\alpha}$ we show the existence of global(almost global) solutions and we calculate the regularity of those solutions.

A Study on Hardiness, Knowledge of Menopause, Menopausal Management among Middle Aged Women (중년여성의 강인성, 폐경지식과 폐경관리에 관한 연구)

  • Shin, Hye-Sook;Kown, Sook-Hee
    • Women's Health Nursing
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    • v.5 no.2
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    • pp.247-261
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    • 1999
  • The purpose of this study was to figure out related factors to the self-reported climacteric symptoms and the relationship among the health promoting behaviors, climacteric symptoms and degree of Sanhujori, the Korean traditional postpartal care. A cross-sectional survey design was employed in this study. The subjects were 108 middle-aged women who were non-hystrectomized and ranged in age from 40 to 60 years. They were selected in seoul and Kyoung-ki province, Korea, Data were collected from Oct.25 Nov. 10, 1997 by a structured questionnaire. The instruments used for this study were the revised health Promotion Lifestyle(HPLP) developed by Walker, Sechrist & Pender, and revised Climacteric Symptoms Scale developed by Chi, Sung Ai. the data were analyzed by the SPSS/$PC^+$ program using t-test, ANOVA and Scheffe test as a post hoc and Pearson Correlation Coefficient. The results of the study were as follows ; 1. The mean score of health promoting behaviors was low($2.42{\pm}0.35$). There were statistically significant differences in the score of health promoting behaviors according to the educational background, family income, marital satisfaction, whether or not taking a restorative food and degree of Sanhujori, especially the period (t=-2.07, F=2.60~7.57, p<0.05). 2. The mean score of score self-reported climacteric symptoms was 1.69%;99% of middle-aged women had symptoms. There were statically significant differences in the score of middle -aged women's self-reported climacteric symptoms according to the age, number of children, educational background, occupation, family income, marital satisfaction, whether or not receiving hormon replacement therapy (HRT) or consultation by a professional, perceived health status and self evaluation of Sanhujori(t=-2.04~3.69, F=2.87~11.63, p<0.05). 3. women's degree of Sanhujori was a positive correlation with health promoting behaviors(r=0.34, p=0.00) and negative correlation with the degree of self-reported climacteric symptoms(r=-0.19,p=0.03). 4. The influencing factors to the climacteric symptoms were self actualization, interpersonal support, and perceived health status among the health promoting behaviors with 57% of variance($R^2$=0.57). 5. The middle-aged women's type of coping pattern for the climacteric symptoms was classified as active behavioral coping, spiritual & psychological coping, and negative coping. In conclusion, to intervene the middle aged women's climacteric symptoms and develop nursing strategies for their health, health promoting behavior, especially ; self actualization, interpersonal support, and perceived health status should be considered. And, as the primary prevention strategy for women's health during the period of childbearing and also middle age, especially for the climacteric symptoms, Sanhujori should be reconsidered.

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Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Olsenella sp. KGMB 04489 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Kim, Ji-Sun;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Yang, Seung-Jo;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.456-459
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    • 2018
  • The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.