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Studies on the Oranization and Expression of tRNA Genes in Aspergillus nidulans (V) The Molecular Structure of $tRNA^{Arg}$ in Aspergillus nidulans (Aspergillus nidulans의 tRNA유전자의 구조와 발현에 관한 연구 V Aspergillus nidulansd의 $tRNA^{Arg}$ 분자구조)

  • 이병재;강현삼
    • Korean Journal of Microbiology
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    • v.24 no.2
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    • pp.79-85
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    • 1986
  • We have determined the sequence of $tRNA^{Arg}$ of A. nidulans partially by enzymatic rapid RNA sequencing technique. The sequence was 5'GGCCGGCUGGCCCAAXUGGCAAGGXUCUGAXUACGAAXCAGGAGAUUGCACXXXXXGAGCXXUXXGUCGGUCACCA3' The cloverleaf structure was made from above data. As a result, the anticodon sequence was identified as ACG. This result was confirmed with charging test. The complete sequence was proposed by supplementing the DNA sequence to and by assigning the position of minor bases to this RNA sequence.

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On the Optimal Adaptive Estimation in the Semiparametric Non-linear Autoregressive Time Series Model

  • So, Beong-Soo
    • Journal of the Korean Statistical Society
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    • v.24 no.1
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    • pp.149-160
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    • 1995
  • We consider the problem of optimal adaptive estiamtion of the euclidean parameter vector $\theta$ of the univariate non-linerar autogressive time series model ${X_t}$ which is defined by the following system of stochastic difference equations ; $X_t = \sum^p_{i=1} \theta_i \cdot T_i(X_{t-1})+e_t, t=1, \cdots, n$, where $\theta$ is the unknown parameter vector which descrives the deterministic dynamics of the stochastic process ${X_t}$ and ${e_t}$ is the sequence of white noises with unknown density $f(\cdot)$. Under some general growth conditions on $T_i(\cdot)$ which guarantee ergodicity of the process, we construct a sequence of adaptive estimatros which is locally asymptotic minimax (LAM) efficient and also attains the least possible covariance matrix among all regular estimators for arbitrary symmetric density.

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Cytotoxicity on Fibroblast Cells of Several Herbicides (몇 가지 제초제가 NIH 3T3 섬유모세포에 끼치는 세포독성)

  • 임요섭;박영민;정연규;한두석;한성수
    • Toxicological Research
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    • v.16 no.2
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    • pp.173-178
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    • 2000
  • This study was carried out to investigate cytotoxicity of several herbicides (Bentazone, Butachlor. Paraquat and Ethalfluralin) in cultured mouse NIH 3T3 fibroblasts. Tetrazolium (MTT), neutral red (NR) and sulforhodamine protein B (SRB) of the colorimetric assays were performed to evaluate the cytotoxicity on cell organelles. 2 x 10$^4$cell/$m\ell$ of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution (1, 25, 50 or 100 $\mu$M) of each herbicide. After the NIH 3T3 fibroblasts of all groups were cultured in the same condition for 48 hours, MTT, NR and SRB assays were performed to evaluate the cytotoxicity. The light microscopic study was carried out to examine morphological changes of cultured NIH 3T3 fibroblasts. The MTT$_{50}$ of Bentazone, Butachlor, Paraquat and Ethalfluralin were 1560.97 $\mu$M, 56.15 $\mu$M, 3138.81 $\mu$M and 1301.82 $\mu$M, respectively. The NR$_{50}$ of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1763.93 $\mu$M, 45.98 $\mu$M, 1030.85 $\mu$M and 1808.29 $\mu$M, respectively. The SRB$_{50}$ of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1913.38 $\mu$M, 65.30 $\mu$M, 1860.73 $\mu$M and 1086.93 $\mu$M, respectively. The morphological changes of NIH 3T3 fibroblasts showed severe degeneration in Butachlor 50 $\mu$M and 100 $\mu$M concentrations. These results indicate that Butachlor has high cytotoxicity, Bentazone, Paraquat and Ethalfluralin very weak cytotoxicity against NIH 3T3 fibroblasts.lasts.

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Prediction Model for the Initial Rotational Stiffness of a Double Split T Connection (상·하부 스플릿 T 접합부의 초기회전강성 예측모델)

  • Yang, Jae-Guen;Kim, Yun;Park, Jae-Ho
    • Journal of Korean Society of Steel Construction
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    • v.24 no.3
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    • pp.279-287
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    • 2012
  • A double split tee connection is used as a connection that is suitable for ordinary moment frames or special moment frames according to the combination of variables of the thickness of the T-stub flange and the gauge distance of the high-strength bolts. In order to demonstrate safe structural behavior, a double split tee connection must meet the requirements for inter-story drift angles and the moment of connection, as defined in the Korea Building Code-Structural. In order to determine whether the these requirements are met, it is necessary to predict rotational stiffness and the ultimate plastic moment of the connection. Therefore, this study primarily aimed to propose an analytical model for predicting the rotational stiffness of a double split tee connection under a static load. Toward this end, a three-dimensional, non-linear finite element analysis was carried out. Then, the applicability of the proposed model was verified after comparing the test results of this study with other studies.

The Influencing Factors on GateKeeper Behavior of Adolescents for Suicide Prevention (청소년의 자살예방을 위한 게이트키퍼 행동 영향요인)

  • Oh, Kyongok;Gang, Moonhee
    • Journal of the Korea Convergence Society
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    • v.8 no.11
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    • pp.431-438
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    • 2017
  • The purpose of the study was to identify the factors on Gatekeeper behavior of adolescents for suicide prevention. The participants were five high school students from D metropolitan city. The collected data were analysed using t-test, ANOVA, Multiple regression with SPSS 21.0 program. In the results of the study, Gatekeeper behavior showed significant difference by gender(t=2.85, p=.005), type of school(t=-2.81, p=.006), experience of significant others' suicide(t=-2.09, p=.038). Gatekeeper behavior had a positive relationship with the perception toward suicide prevention(r=.23, p=.006) and the knowledge about suicide prevention(r=.42, p<.001). In regression analysis, significant influencing factors on Gatekeeper behavior were the perception toward suicide prevention (${\beta}=.20$, p=.006) and the knowledge about suicide prevention(${\beta}=.35$, p<.001), and these variables showed 24.3% of variance. Therefore, it need to develop the intervention for improving positive perception and active knowledge about suicide prevention in adolescents. In addition, future study to identify various predictable variables is needed.

Effects of Different Levels of Concentrate in the Diet on Physicochemical Traits of Korean Native Black Goat Meats

  • Kim, Sang Woo;Park, Seong Bok;Kim, Myeong Jik;Kim, Dong Hun;Yim, Dong-Gyun
    • Food Science of Animal Resources
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    • v.34 no.4
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    • pp.457-463
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    • 2014
  • The effects of feeding of diets based on roughage supplemented with concentrate on the carcass and meat quality attributes of Korean black goats were investigated. The 40 male goats at 5 mon age were divided into four treatment groups; T1 (1.5%), T2 (2.0%), T3 (2.5%) and T4 (ad libitum) feeding of concentrate with ad libitum rice straw. Forty bucks at the end of the feeding were fasted for 12 h before slaughter and were then slaughtered. For carcass measurement, cold buck carcasses were examined after 24 h of chilling at $5^{\circ}C$. The carcasses were chilled in refrigerator at $2{\pm}2^{\circ}C$ for 24 h, then the longissimus dorsi muscle was removed from the carcasses. The samples were separately vacuum packaged, frozen and stored at $-20^{\circ}C$ for up to 1 mon, prior to physicochemical evaluations. Carcass yields were greatly affected by rate of concentrate feeding. T4 showed the heaviest live and carcass weights and the highest dressing and fat percentages (p<0.05). The moisture contents were lower in T4 compared with other treatments, while the crude protein and fat contents were higher (p<0.05). mono-unsaturated fatty acid in T4 was significantly higher than others, which showed the highest percentages of oleic acid (C18:1). For the sensory test, T4 was tenderer and produced better flavor scores than others. Increasing the level of concentrate in the diet resulted in an improvement in growth performance, and carcass and physicochemical characteristics of goat meats.

Comparison of Soil Characteristics and Carbon Storage between Urban and Natural Lands - Case of Chunchon - (도심지와 자연지간 토양 특성 및 탄소저장량 비교 - 춘천시를 대상으로 -)

  • Jo, Hyun-Kil;Han, Gab-Soo
    • Journal of Forest and Environmental Science
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    • v.15 no.1
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    • pp.71-76
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    • 1999
  • This study compared soil characteristics and carbon storage between urban and natural lands in Chunchon. Soil pH was lower in natural lands (5.0) than in urban lands (6.6), and therefore exchangeable cation was a little lower in natural lands. Organic matter and cation exchange capacity were respectively, 1.4 and 1.7 times higher in natural lands than in urban lands, while available $P_2O_5$ was about 3.2 times higher in urban lands. Organic carbon storage in soils averaged $24.8{\pm}1.6$ (standard error) t/ha in urban lands and $31.6{\pm}1.6t/ha$ in natural lands, 1.3 times greater than in urban lands. Annual carbon accumulation in soils of natural lands was 1.3 t/ha/yr (litterfall minus decomposition). The carbon storage in Chunchon' s soils equaled about 31% of annual carbon emission (245,590 t/yr).

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Biomedical Ethics Education for Nursing Students: The Effect on Awareness and Application of Nursing Code of Ethics, Consciousness of Biomedical Ethics and Moral Sensitivity (생명의료윤리 교육 프로그램이 간호 대학생의 간호사 윤리강령인식, 간호사 윤리강령 활용 정도, 생명의료 윤리 의식 및 도덕적 민감성에 미치는 효과)

  • Choi, Young-Sil;Jeong, Gye-Seon;Rayborn, Michong
    • The Journal of Korean Academic Society of Nursing Education
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    • v.24 no.3
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    • pp.214-224
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    • 2018
  • Purpose: This study examines the effectiveness of an educational program of nursing biomedical ethics for nursing students to increase awareness of the Code of Ethics for Nurses, moral sensitivity, and application of the Code of Ethics for Nurses. Methods: A quasi-experimental research design and t-test were used. The experimental group (n=31) and control group (n=32) were second-year university nursing students in two major cities. The experimental group received 15 hours of nursing ethics education during the 15-week semester. The training was not provided to the control group. Results: The application of the Code of Ethics for Nurses increased significantly in the experimental group (t=-1.06, p=.017), nurses and patient (t=-2.23, p=.029), obligation of nurse as an expert (t=-2.08, p=.042), nurse and cooperator (t=-2.54, p=.014). The consciousness of biomedical ethics increased significantly in the experimental group (t=4.28, p=.021), newborn's right to live (t=-2.61, p=.011), euthanasia (t=-2.36, p=.021). Conclusion: The results of the study show that providing a nursing biomedical ethics program to nursing students is an effective method to enhance the application of the Code of Ethics for Nurses and Consciousness of Biomedical Ethics. Implementing an intervention program of the Code of Ethics for Nurses in the regular nursing curriculum may reduce conflicts involving ethical decision making by nurses.

Anti-adipogenic Effect of Mori Follium Extract in 3T3-L1 Cells (상엽(桑葉) 추출물의 Adipogenesis 억제를 통한 항비만 활성 평가)

  • Kwon, O Jun
    • The Korea Journal of Herbology
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    • v.31 no.5
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    • pp.47-53
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    • 2016
  • Objectives : Mori Follium (Morus alba L. leaf) has been cultivated in many Asian countries. Especially, mulberry leaf has been used as an anti-diabetic remedy in oriental medicine. However, anti-obesity effect of mulberry has not been unknown. In this study, our objectives of study is to investigate the anti-adipogenic effect of mulberry water extract (MLE) and to reveal potential molecular anti-obesity mechanism in 3T3-L1 adipocytes differentiation model.Methods : The cytotoxicity of MLE in 3T3-L1 was examined by MTT assay. Anti-adipogenic effect of MLE was evaluated by Oil Red O (ORO) staining. To elucidate the molecular mechanism, inhibitor assay was employed. The mRNA expression levels of adipogenic transcriptional factors such as PPARγ and fatty acid synthase (FAS) were analyzed by reverse transcription-polymer chain reaction (RT-PCR) analysis.Results : The MLE treatment for 24 h did not affect to the 3T3-L1 cells at concentrations of 1, 10, 100, 200, 400, 800 and 1,000 ㎍/㎖. Thus, non-toxic concentration rages of MLE were used during adipogenesis period (day -2 to 7). Intracellular lipid accumulation in MLE-treated 3T3-L1 adipocytes (day 6) were quantitatively evaluated by ORO staining. The MLE treatment significantly and dose-dependently suppressed 3T3-L1 adipogenesis by 60.42%, 38.24%, and 5.97% at 10, 100, and 200 ㎍/㎖, respectively. In addition, our inhibitor assay and RT-PCR analysis revealed that the MLE-inhibited 3T3-L1 adipogenesis through inhibition of PPARγ mediated by Wnt/β-catenin signaling pathway.Conclusions : In conclusion, these findings indicate that the MLE could be used in prevent and/or treatment of obesity-related diseases.