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Residue level and pharmacokinetics of trichlorfon in the Japanese eel (Anguilla japonica) after bath treatment (Trichlorfon (TCF)의 약욕 투여에 따른 뱀장어 체내 약물 잔류량 및 약물동태학 연구)

  • Jo, Hyun Ho;Chung, Joon Ki
    • Journal of fish pathology
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    • v.35 no.1
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    • pp.93-102
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    • 2022
  • This study performed a trichlorfon (TCF) residue and pharmacokinetic analysis with Japanese eels, Anguilla japonica, to obtain baseline data to establish the maximum residue level (MRL) of TCF in A. japonica. After dipping A. japonica in 30 ppm and 150 ppm of TCF at 28℃ and 18℃, drug residue in the body was analyzed with LC-MS/MS, and these results were further analyzed with the PK solver program to obtain the pharmacokinetic parameters of TCF in the serum, muscles, and liver. The maximum concentrations (Cmax) in the serum, muscles, and liver were 25.87-357.42, 129.91-1043.73, and 40.47-375.20, respectively, and the time to maximum concentration (Tmax) was 0.13-1.32h, 1.17-3.34h, and 0.14-5.40h, respectively. The terminal elimination half-life (T1/2) was 2.13-3.92h, 5.30-10.35h, and 0.65-13.81h, respectively. In the 30 mg/L concentration group, TCF was not detected in the serum of eels 96 hours after bathing, and was below the detection limit after 336 hours in muscle and liver. On the other hand, in the 150 mg/L concentration group, TCF was not detected in the serum of eels 336 hours after bathing, but was detected in muscle and liver at 336 hours. In conclusion, the results of this study would be useful in establishing the MRL of TCF in farmed A. japonica.

A Low-Cost Portable Precise Position Information Service System Using the DGPS Mechanism (DGPS 개념을 이용한 저가형 이동식 정밀위치 서비스 시스템)

  • Yeoun Hyo-Bum;Kang Yeong-Wook;Lee Ki-Dong
    • The KIPS Transactions:PartC
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    • v.13C no.1 s.104
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    • pp.95-102
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    • 2006
  • Nowadays, GPS is used widely, especially in case which needs precise position information, such as car navigation systems and various kinds of position measuring instruments in an outdoor environment. According to their applications, there are many kinds of GPS receivers with different costs and error rates. The maximum error range of the general-purpose GPS receiver is within 30m, though the error rate depends on receiving rate of signal and weather condition. RTK(Real-Time Kinematic) and DGPS(Differential Global Positioning System) have more precise accuracy than the general-purpose GPS. However end users can't afford use them because of their high price and large size of equipments. In order for the end user to obtain precise position information, it is important that GPS receivers has portability and low price. In this study, we introduce a new system that offers precise position information using the DGPS mechanism satisfying low cost and portability.

Investigation on antimicrobial resistance genes of Salmonella spp. isolated from pigs and cattle (소와 돼지유래 살모넬라속균의 약계내성유전자의 특성에 관한 연구)

  • Lee, Woo-Won;Jung, Byeong-Yeal;Lee, Gang-Rok;Lee, Dong-Soo;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.227-239
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    • 2009
  • At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM ($bla_{TEM}$) gene was detected from 51 (92.7%) of 55 $\beta$-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 $\beta$-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sulII (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

Determination of Total CO2 and Total Alkalinity of Seawater Based on Thermodynamic Carbonate Chemistry (해수중의 총이산화탄소와 총알칼리도 분석을 위한 탄산염 화학 이론 및 측정방법)

  • Mo, Ahra;Son, Juwon;Park, Yongchul
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.18 no.1
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    • pp.1-8
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    • 2015
  • To evaluate accuracy and precision of determination of total alkalinity ($Alk_T$) and carbon dioxide ($TCO_2$) derived from present study, experiment was applied with $CO_2$ CRM (Batch 132, Scripps Institution of Oceanography; $Alk_T=2229.24{\pm}0.39{\mu}mol/kg$, $TCO_2=2032.65{\pm}0.45{\mu}mol/kg$). As the result, average concentration of $Alk_T$ and $TCO_2$ was $2354.09{\mu}mol/kg$ (~5.6% difference with $CO_2$ CRM) and $2089.60{\mu}mol/kg$ (~2.3% difference with $CO_2$ CRM), respectively. For previous method (Gran Titration) by addition $NaHCO_3$ to deionized water($Alk_T$ $2023.33{\mu}mol/kg$), average concentration was $2193.39{\mu}mol/kg$ (sd=57.15, n=7). Whereas, average concentration was $2017.02{\mu}mol/kg$ (sd=10.98, n=7) for the present study. Recovery yield experiments of total alkalinity in deionized water and seawater were implemented by addition of $NaHCO_3$. The recovery yield of deionized water in the range 0 to $4952.39{\mu}mol/kg$ was 100.8% ($R^2$=0.999), and seawater in the range 0 to $2041.32{\mu}mol/kg$ was 102.3% ($R^2$=0.999). Comparison of $pCO_2$ sensor (PSI $CO_2-Pro^{TM}$) with present method showed very meaningful correlation coefficient ($R^2$=0.977) in the range of 427 to $705{\mu}atm$ and 9.16 to $15.24{\mu}mol/kg$ throught elapsed time for two weeks. Field experiment of diurnal variation of total carbon dioxide was accomplished at Sachon harbor in the coastal waters of East Sea of Korea. Concentration of $Alk_T$ and $TCO_2$ was increased during night, and decreased during daylight hours. The results showed mirror type between $TCO_2$ and dissolved oxygen, which was attributable to photosynthesis and respiration of phytoplankton. Also, open ocean field study was performed to obtain vertical profile of $Alk_T$ and $TCO_2$ in C-C zone (Clarion-Clipperton Fracture Zone), Northeastern Pacific. Average concentrations of $Alk_T$ in the surface mixed layer (0~60 m) and deeper layer below 200 m were $2422.38{\mu}mol/kg$ (sd=78.73, n=20) and $2465.87{\mu}mol/kg$ (sd=57.68, n=103), respectively. And average concentrations of $TCO_2$ were $2134.47{\mu}mol/kg$ (sd=65.4, n=20) and $2431.87{\mu}mol/kg$ (sd=65.02, n=103) in the same depth ranges such as $Alk_T$. Vertical distributions of $Alk_T$ and $TCO_2$ concentrations tended to increase with depth, and analyzed concentrations showed slightly higher than those of previous studies in this area.

The Dielertric and Piezoelectric Properties of PZT-PCNS Piezoelectric Ceramics with Zr/Ti Mole Ratio (Zr/Ti 몰비에 따른 PZT-PCNS 압전 세라믹의 압전 및 유전 특성)

  • Lee, S.Y.;Lee, Y.H.;Lee, M.J.;Lee, J.S.;Lim, K.J.
    • Proceedings of the KIEE Conference
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    • 2001.11a
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    • pp.100-102
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    • 2001
  • In this paper, the piezoelectric and dielectric properties of $Pb(Zr_xTi_{1-x)O_3$ - $Pb(Co_{1/3}Nb_{1/3})O_3)$ piezoelectric ceramics have been investigated as a function of Zr/Ti mole ratio. From the results, when Zr/Ti mole ratio is 49/51, electromechanical coupling coefficient($k_p$), piezoelectric strain constant($d_{33}$) mechanical quality factor($Q_m$), and Permittivity(${{\varepsilon}_{33}}^T/{\varepsilon}_0$) is 64[%], 469[PC/N], 360 and 2000, respectively. Morphotropic Phase Boundary is Zr/Ti mole ratio(49/51) from XRD analysis. Also. From SEM observation, when sintering temperature is 1150[$^{\circ}C$], grain size is about $1{\sim}2[{\mu}m]$ and maximum sintering density is 7.85[$g/cm^3$].

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A Study on the Bending Process for the Curved Tube by Hot Metal Extrusion Machine with the Multiple Punches Moving in the Different Velocity (다지형 압출펀치의 상대이동 속도 차이에 의한 금속 곡관의 열간금속 압출굽힘가공에 관한 연구)

  • Park D. Y.;Jin I. T.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2001.05a
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    • pp.102-105
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    • 2001
  • The bending process for the curved tube can be developed by the hot metal extrusion machine with the multiple punches moving in the different velocity. The bending phenomenon has been studied to be occurred by the different of velocity at the die extrusion. The difference of velocity at the die exit section can be obtained by the different velocity of billets through the multi-hole container and by the welding of billets inside the porthole die chamber. The multiple billets are moving differently by the multiple extrusion punches controlled by PLC with the servo mechanism units. The results of the experiments show that the curved tube can be bended by the extrusion process and that the defects such as the distortion of section and the thickness change of thick tube, tile folding and wrinkling of thin tube can not be shown after the bending processing by the extrusion bending machine.

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A Study on the Interactive Grain Stability Calculation (대화형 Grain Stability Calculation에 관한 연구)

  • Lee, S.S.;Lee, K.O.;Kang, W.S.;Yoon, M.T.;Sung, D.K.;Lee, J.C.
    • Journal of the Society of Naval Architects of Korea
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    • v.34 no.1
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    • pp.102-110
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    • 1997
  • In a shipyard, computer calculation is not frequently used for the grain stability calculation because of large difference between calculation values and real values. Therefore, the necessary calculation process for grain stability is done manually. GUI(Graphical User Interface) is adopted for the convenience of users and interactive data I/O. The hold shape (girder, hold, etc.)needed for calculation are visualized using GLBAX which is a 3 dimensional graphic library. The interface with the ship basic calculation package is also implemented. The aim of this paper is to develop a reliable interactive grain stability calculation program which reduces computational time, and is to computerize the grain stability calculation procedure.

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Voltage Sag-Swell Generator for Power Quality Disturbance of Dynamic UPS System (다이나믹 UPS 시스템의 전력품질 외란발생을 위한 전압 Sag-Swell 발생기)

  • Byeon W. Y.;Kim J. W.;Lee K. S.;Nho E. C.;Kim I. D.;Chun T. W.;Kim H. G.
    • The Transactions of the Korean Institute of Power Electronics
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    • v.10 no.1
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    • pp.102-107
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    • 2005
  • This paper describes a new voltage sag-swell generator for the test of custom power devices such as UPS, DVR, DSTATCOM, SSTS, etc. Voltage sag, swell, outage, and unbalance generation mechanism and the operating principle are described for the proposed scheme. The usefulness of the scheme is proved through simulations and experiments. The proposed scheme has good features of simple structure, high reliability, wide range of sag and swell variation, and easy control. Especially, the scheme can provide a cost-effective implementation of a power quality disturbance generator. Therefore, it is expected that the scheme will contribute to the self implementation of the system with low cost in laboratory.

Influences of Personality and Positive Psychological Capital on Academic Resilience of Nursing Students (간호대학생의 인성, 긍정심리자본이 학업탄력성에 미치는 영향)

  • Jeong-Min Lim
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.6_2
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    • pp.1033-1046
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    • 2023
  • The purpose of this study was to investigate the relationship between personality, positive psychological capital, and academic resilience of nursing students and to identify the factors affecting academic resilience of nursing students. Data were collected from 199 nursing students in their first and second year at C nursing college in M-gun, J-do, and a survey was conducted from July 10 to July 28, 2023. The collected data were analyzed using descriptive statistics, t-test, one-way ANOVA, Scheffe test, Pearsons correlation coefficient, Stepwise multiple regression analysis was conducted. Personality had a significant positive correlation with positive psychological capital(r=.62, p<.001) and academic resilience(r=.68, p<.001), and positive psychological capital had a significant positive correlation with academic resilience(r=.79, p<.001). The major factor influencing the academic resilience of nursing students was positive psychological capital, followed by personality, interpersonal relationship, and college life satisfaction, and their total explanatory power was 72% (F=102.85, p<.001). Based on the results of this study, it is necessary to develop an educational program that can increase positive psychological capital in order to strengthen the academic resilience of nursing students, and efforts should be made to introduce and manage it in the nursing curriculum.

Complete genome sequence of Pediococcus acidilactici CACC 537 isolated from canine

  • Jung-Ae Kim;Hyun-Jun Jang;Dae-Hyuk Kim;Youn Kyoung Son;Yangseon Kim
    • Journal of Animal Science and Technology
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    • v.65 no.5
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    • pp.1105-1109
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    • 2023
  • Pedi coccus acidilactici CACC 537 was isolated from canine feces and reported to have probiotic properties. We aimed to characterize the potential probiotic properties of this strain by functional genomic analysis. Complete genome sequencing of P. acidilactici CACC 537 was performed using a PacBio RSII and Illumina platform, and contained one circular chromosome (2.0 Mb) with a 42% G + C content. The sequences were annotation revealed 1,897 protein-coding sequences, 15 rRNAs, and 56 tRNAs. It was determined that P. acidilactici CACC 537 genome carries genes known to be involved in the immune system, defense mechanisms, restriction-modification (R-M), and the CRISPR system. CACC 537 was shown to be beneficial in preventing pathogen infection during the fermentation process, help host immunity, and maintain intestinal health. These results provide for a comprehensive understanding of P. acidilactici and the development of industrial probiotic feed additives that can help improve host immunity and intestinal health.