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돼지 Phosphatidylinositol Glycan, Class K (PIGK) 유전자의 동정과 양적형질과의 연관성 분석 (Characterization of Phosphatidylinositol Glycan, Class K (PIGK) Gene and Analysis of Association with Quantitative Traits in Pigs)

  • 임현태;김재환;최봉환;이상호;박응우;김태헌;조인철;오성종;이정규;전진태
    • Journal of Animal Science and Technology
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    • 제47권2호
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    • pp.167-176
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    • 2005
  • PIGK(phosphatidylinositol glycan, class K) is a subunit of GPI transamidase that cleaves the signal peptide in proproteins and replaces it with GPI. In addition, the structure and synthesis of GPI are critically involved in some of the cellular actions of insulin. Therefore, PIGK would be essential for mammalian development and many specific cellular functions as well as for metabolic activity of insulin associated with GPI. Two types of" full-length cDNAs of porcine PIGK were cloned through RT-PCR and RACE experiments. One is thought to be a normal form(consist of 395 amino acids) and the other is considered as an alternative spliced form(consist of 371 amino acids) which contains additional 63 bps in intron 7. Since a stop codon was contained within the insertion, the spliced form has a shorter coding sequence than that of normal form. A missense mutation (T314I) in exon 6 was detected and used for genotyping to estimate association with the growth and fat deposition traits for 545 $F_2$ animals(Korean native boars ${\times}$ Landrace). From the PCR-RFLP analysis using HpyCH4III, CT genotype showed highly significant relationship(P< 0.01) with carcass fat contents.

방선균으로부터 Phospholipase D 생산균주의 탐색 (Screenin of Phospholipase D Producing Actinomycetes)

  • 손동화;심재용;윤석후
    • 한국미생물·생명공학회지
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    • 제22권4호
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    • pp.333-339
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    • 1994
  • In order to screen microorganisms producing phospholipase D (PLD) [EC 3.1.4.4], culture broths of about 900 strains of soil bacteria were subjected to examine for the PLD activity. When the hydrolytic activity of PLD (H-activity) in the supernatant was determined, 64 strains produced PLD more than 0.3 unit/ml and all of them were actinomycetes. Among 26 culture broths tested, 6 ones had transphosphatidylation activity (T-activity) of 30~68%. When the strains except one were cultivated on 3 different media at 30$\circ$C for 3 days under aerobic condition, strain # 1090 on medium B (yeast extract 1%, peptone 1%, glucose 1.5%, glycerol 1%, CaCO$_{3}$ 0.4%, and pH 7.2) produced PLD with much higher H- and T-activity, which were 8.3 units/ml and 76.3%, respectively. Subsequently, time course of PLD production of the strain # 1090 during cultivation with aeration of 1 v/v/m and agitation of 400 rpm at 30$\circ$C for 5 days on medium B in jar fermentor was investigated. H-activty of PLD reached almost maximum (about 9 units/ml) after 32 hours and maximal T-activity was found to be about 80%.

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TOPOLOGICAL ENTROPY OF EXPANSIVE FLOW ON TVS-CONE METRIC SPACES

  • Lee, Kyung Bok
    • 충청수학회지
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    • 제34권3호
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    • pp.259-269
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    • 2021
  • We shall study the following. Let 𝜙 be an expansive flow on a compact TVS-cone metric space (X, d). First, we give some equivalent ways of defining expansiveness. Second, we show that expansiveness is conjugate invariance. Finally, we prove that lim sup ${\frac{1}{t}}$ log v(t) ≤ h(𝜙), where v(t) denotes the number of closed orbits of 𝜙 with a period 𝜏 ∈ [0, t] and h(𝜙) denotes the topological entropy. Remark that in 1972, R. Bowen and P. Walters had proved this three statements for an expansive flow on a compact metric space [?].

Technology Development of Early Diagnosis for Pregnancy in Deer

  • Lee, J. H.;Park, S.J.;Ryu, I. S.;Kim, I.C.;Lee, D.W.;Seo, K.H.;Heo, T.H.;Yu, C.H.;Kim, C.K;Baek, S.H.;Son, D.S.
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.81-81
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    • 2002
  • The deer was inseminated at the breeding season, which only shows appearance of the estrus cycles. Early diagnosis of pregnancy is very useful to select non-pregnant does and also very important to decide re-inseminations or mating for producing of calves. Therefore, for the development of techniques on the early diagnosis of pregnancy of deer, the results of this study were summarized as following. (omitted)

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