• Title/Summary/Keyword: T. granosa

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Temperature Tolerance and Physiological Changes of Blood Cockle, Tegillarca granosa (꼬막, Tegillarca granosa의 수온내성과 생리적 변화)

  • SHIN Yun Kyung;MOON Tae-Seok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.4
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    • pp.251-256
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    • 2005
  • For blood cockle Tegillarca granosa acclimated to winter and summer seasons, survival, temperature tolerance and physiological changes at different individual size were investigated for their temperature tolerances by increasing and decreasing temperature at a rate of 1, 2 and $3^{\circ}C/day$. The survival rate of adults and juveniles T. granosa acclimated to winter temperatures began to decrease from $32^{\circ}C$ and all experimental animals died between $37-39^{\circ}C$. In the case of animals acclimated to summer temperatures, the survival rates of adults and juveniles began to decrease from $35^{\circ}C$, and all died at temperatures between $40-44^{\circ}C$. The upper $LT_{50}$ was $27.72^{\circ}C$ for adults and $28.36^{\circ}C$ for juveniles. On the other hand, when the temperature was decreased from $4^{\circ}C\;to\;0^{\circ}C$ in order to investigate lower temperature tolerances, the survival rate of T. granosa was more than $70\%\;at\;2^{\circ}C$ for 25 days. Lower L T 50 was $2.09^{\circ}C$ for adults and $2.34^{\circ}C$ for juveniles. There was no effective difference in temperature tolerance between adults and juveniles. Filtration and respiration rates of T. granosa showed a similar aspect with increase and decrease of temperature. Filtration and respiration rates exhibited irregular conditions of a broken biological rhythm as the group acclimated to winter $(10 ^{\circ}C)$ and summer $(25^{\circ}C)$. In the case of decreasing temperature, filtration and respiration rates of T. granosa reduced to a minimum below $6^{\circ}C$

Indirect Evidence on Sex Reversal with Sex Ratio of Tegillarca granosa (Bivalvia: Arcidae) and Ruditapes philippinarum (Bivalvia: Veneridae)

  • Lee, Jung-Sick;Ku, Ka-Yeon;Kim, Hye-Jin;Park, Ji-Seon;Park, Jung-Jun;Shin, Yun-Kyung;Jeon, Mi-Ae
    • Development and Reproduction
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    • v.16 no.3
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    • pp.177-183
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    • 2012
  • This study attempts to propose the possibility of the sex reversal in Tegillarca granosa and Ruditapes philippinarum by confirming the changes in the sex ratio with the shell length (SL) in the same population level. For analysis of sex ratio with SL, 1500 individuals of T. granosa (SL 10.1-45.0 mm) and 712 individuals of R. philippinarum (SL 15.1-70.0 mm) were used. Sex was analyzed histologically. The average sex ratios (F:M) of T. granosa and R. philippinarum were 1:1.22 and 1:0.96, respectively. However, sex ratio was found to differ when the clams were divided into groups according to SL in 5.0 mm intervals. Both species displayed the tendency of increase in the proportion of female with increase in SL. In this study, changes in the sex ratio in accordance with the growth of T. granosa and R. philippinarum are determined to be indirect evidence that signifies their sex reversal.

Genome Survey and Microsatellite Marker Selection of Tegillarca granosa (꼬막(Tegillarca granosa)의 유전적 다양성 분석을 위한 드래프트 게놈분석과 마이크로새틀라이트 마커 발굴)

  • Kim, Jinmu;Lee, Seung Jae;Jo, Euna;Choi, Eunkyung;Kim, Hyeon Jin;Lee, Jung Sick;Park, Hyun
    • Journal of Marine Life Science
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    • v.6 no.1
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    • pp.38-46
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    • 2021
  • The blood clam, Tegillarca granosa, is economically important in marine bivalve and is used in fisheries industry among western Pacific Ocean Coasts especially in Korea, China, and Japan. The number of chromosomes in the blood clam is known as 2n=38, but the genome size and genetic information of the genome are not still clear. In order to predict the genomic size of the T. granosa, the in-silico analysis analysed the genomic size using short DNA sequence information obtained using the NGS Illumina HiSeq platform. As a result, the genomic size of T. granosa was estimated to be 770.61 Mb. Subsequently, a draft genome assembly was performed through the MaSuRCA assembler, and a simple sequence repeat (SSR) analysis was done by using the QDD pipeline. 43,944 SSRs were detected from the genome of T. granosa and 69.51% di-nucleotide, 16.68% trinucleotide, 12.96% tetra-nucleotide, 0.82% penta-nucleotide, and 0.03% hexa-nucleotide were consisted. 100 primer sets that could be used for genetic diversity studies were selected. In the future, this study will help identify the genetic diversity of T. granosa and population genetic studies, and further identify the classification of origin between homogenous groups.

Changes in planktonic bivalve larvae of Tegillarca granosa and Anadara kagoshimensis in the Boseong coastal waters of South Korea (보성 연안해역에서 꼬막과 새꼬막 부유유생 출현의 변화)

  • Kim, Hyun-Jung;Kang, JunSu;Jung, Seung Won;Park, Yong-Joo
    • Korean Journal of Environmental Biology
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    • v.37 no.3
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    • pp.351-361
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    • 2019
  • To investigate the temporal distribution of planktonic larvae of Tegillarca granosa and Anadara kagoshimensis in the Boseong coastal waters of South Korea, samples of planktonic bivalve larvae were taken from the coastal waters from June to September 2018 (this consisted of monthly sampling in June, July, and September with three- or four-day interval sampling in August). The samples were analyzed using metagenomic next-generation sequencing methods (target gene: mitochondria cytochrome c oxidase 1 region). In this study, a total of 21 bivalve operational taxonomic units (OTUs) were detected with the most abundant bivalve OTUs (relative mean abundance >1%) belonging to Magallana sikamea, Xenostrobus atratus, Musculista senhousia, Magallana gigas, Sinonovacula constricta, Anadara kagoshimensis, Kurtiella aff. bidentata, and Tegillarca granosa. In particular, Tegillarca granosa and Anadara kagoshimensis (the main fishery resources on the Boseong coast) accounted for 0.51-12.50% (average 4.00%) and 0.01-12.50% (1.92%), respectively. The planktonic bivalve larvae were most abundant from July to August. Anadara kagoshimensis was most abundant in early August but rare in the other investigated periods, whereas Tegillarca granosa was more abundant in late August. Bivalve larvae monitoring is important to predict the production of bivalve fisheries. Therefore, intensive monitoring is needed to understand the changes in planktonic bivalve larvae because potentially rapid turnover can respond to the ecological interaction of spawning bivalves.

Gonadal Development and Reproductive Cycle of the Granular Ark Tegillarca granosa (Bivalvia: Arcidae) (꼬막 Tegillarca granosa의 생식소 발달과 생식주기)

  • Kim, Sung-Yeon;Moon, Tae-Seok;Shin, Yun-Kyung;Park, Mi-Seon
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.34-41
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    • 2009
  • Gonadal development and reproductive cycle of the granular ark Tegillarca granosa were investigated by histological observations. Samples were collected monthly from January to Decemberry 2002 in the Yeoja Bay of Yeosu, Jeollanam-do, Korea. T. granosa was dioecious. The gonads consist of a number of oogenic follicle and acinus. Monthly changes in the gonad index reached a maximum in June and a minimum in August. Monthly changes in the condition index reached a maximum in July and a minimum in September. The reproductive cycle of this species can be divided into five successive stages: early active stage (March to May), late active stage (April to June), ripe stage (May to July), spent stage (July to August) and recovery and resting stage (September to March). The spawning of T. granosa occurred in July and August in Yeoja Bay. The sex ratio of female to male was not significantly different from 1:1.

Digestive Enzymatic Compositions and Activities of the Digestive Diverticula in Three Species of Bivalves (이매패류 3종 소화맹낭의 소화효소 구성 및 활성도)

  • Ju, Sun-Mi;Kwon, O-Nam;Lee, Jung-Sick
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.371-376
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    • 2011
  • Digestive enzymatic compositions and activities in digestive diverticula of the three species of bivalves were investigated in this study. Mytilus galloprovincialis, Saxidomus purpuratus and Tegillarca granosa which were collected from southern coast of Korea on November 2010, were used for analysis. Amylase and cellulase occupied approximately 95% of digestive enzymes in digestive diverticula of M. galloprovincialis, S. purpuratus and T. granosa. The amylase and cellulase activities were 2.6 and 0.8 U/mg in M. galloprovincialis, 2.4 and 8.8 U/mg in S. purpuratus and 7.3 and 11.8 U/mg in T. granosa. And protease activities in digestive diverticula of M. galloprovincialis, S. purpuratus and T. granosa showed the lowest values to 0.00019, 0.00028 and 0.00022 U/mg, respectively.

Shell Height Frequency using of Age and Growth of Blood Cockle, Tegillarca granosa (Linnaeus) in Yeoja Bay, Southern Coast of Korea (각고조성을 이용한 남해안 여자만 꼬막의 연령과 성장)

  • Chang, Dae-Soo;Moon, Tae-Seok;Jung, Min-Min
    • The Korean Journal of Malacology
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    • v.22 no.2
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    • pp.135-141
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    • 2006
  • This paper describes relative age and growth pattern of the blood cockle, Tegillarca granosa (Linnaeus) in Yeoja Bay, southern coast of Korea. The young shell of blood cockle that spawned from July to August reached 5.60 mm $({\pm}\;1.07)$ in shell height in October. Slow growing season was estimated to be from October to the next May with water temperature under $23^{\circ}C$. With warm water temperature in July to September, the growth was fast. The young shells reached 29 percentage of asymptotic shell height 11-15 months after spawning. Growth was estimated by von Bertalanffy growth function as follows. $I_t(sh_t)=46.1317\;(1-e^{-0.4997(t-0.5828)})$.

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Effects of the Dissolved Oxygen Concentration on the Physiology of the Manila clam, Teillarca granosa (Linnaeus) (꼬막, Tegillarca granosa (Linnaeus)의 용존산소 변화에 따른 생리적 반응)

  • Shin Yun Kyung;Moon Tae Seok;Wi Chong Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.485-489
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    • 2002
  • To investigate the effects of the dissolved oxygen concentration (DO) of Tetillarca granosa (Linnaeus), We measured $LC_{50}$, survival, oxygen consumption rate, filtration rates and blood composition under $10{\cdot}{\pm}0.5^{\circ}C$ and $25{\cdot}0.5^{\circ}C$ as a function of DO. The 16 days-$LC_{50}$ of DO for T. granosa was 1.31 mg DO/L in large individual and 0.95 mg DO/L in small individual. At 25$^{\circ}C$, the $LC_{50}$ of DO in large and small individual was 1.13 and 1.M mg DO/L, respectively. With decreasing DO, oxygen consumption rate, and filtration rates of T. granosa decreased. Blood composition of T. granosa was analysed hemoglobin, glucose, total protein, total cholesterol, GOT and GPT, Hg was increased with decreasing DO, but glucose was decreased below 1.2 mg DO/L. Total protein, total cholesterol, GOT and GPT were investigated irregular and decreasing aspect.

A Novel Anticoagulant Protein with High Affinity to Blood Coagulation Factor Va from Tegillarca granosa

  • Jung, Won-Kyo;Jo, Hee-Yeon;Qian, Zhong-Ji;Jeong, Young-Ju;Park, Sae-Gwang;Choi, Il-Whan;Kim, Se-Kwon
    • BMB Reports
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    • v.40 no.5
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    • pp.832-838
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    • 2007
  • A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESI-QTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner ($IC_{50}$ value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa ($FVa_H$) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).

Digestive Enzyme Activity within Crystalline Style in Three Species of Bivalves (이매패류 3종의 당면체 소화효소 활성)

  • Ju, Sun-Mi;Kwon, O-Nam;Kim, Jae-Won;Lee, Jung-Sick
    • The Korean Journal of Malacology
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    • v.27 no.1
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    • pp.9-14
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    • 2011
  • This study was examined digestive enzyme activity in the crystalline style of the three species of bivalves. Bivalves used in this study were Tegillarca granosa (n=61), Mytilus galloprovincialis (n=30) and Saxidomus purpuratus (n=30) and collected from southern coast of Korea on May 2010. Digestive enzymes activities in the crystalline style were assayed in spectrophotometer. Amylase and cellulase occupied approximately 90% of digestive enzyme in crystalline style of T. granosa, M. galloprovincialis, and S. purpuratus. And protease activity in crystalline style of T. granosa, M. galloprovincialis and S. purpuratus showed the lowest values to 0.02, 0 and 0.08%, respectively. Digestive enzyme activity in crystalline style of three species was measured in the order of cellulase > amylase > chitinase > laminarinase.