• Natural Product Sciences
• /
• v.16 no.4
• /
• pp.280-284
• /
• 2010

During liver fibrosis, hepatic stellate cells (HSCs) undergo a complex activation process characterized by increased proliferation and extracellular matrix deposition, which is the major pathological feature of hepatic cirrhosis. Therefore, suppression of HSCs activation has been proposed as therapeutic strategies for hepatic fibrosis. We tried to screen the antifibrotic activity of natural products employing HSC-T6, hepatic stellate cell lines as an in vitro assay system. In the present study, we investigated the antiproliferative activity of aerial parts and roots of Pulsatilla koreana Nakai (Ranunculaceae). Our present study shows that roots of P. koreana exerted more strong inhibitory activity compared to its aerial parts. In addition, among the fractions of the aqueous methanolic extract of P. koreana roots, both n-hexane and $CHCl_3$ fraction showed the strong inhibitory activity on HSC proliferation. Further study also demonstrated that the n-hexane and $CHCl_3$ fraction of P. koreana roots significantly inhibited the HSC proliferation in time- and concentration-related manners.

• Korean Journal of Plant Resources
• /
• v.29 no.3
• /
• pp.339-346
• /
• 2016

This study was carried out to develop an effective seed propagation method for Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai by analyzing seed dormancy types and germination characteristics. Seeds were collected between September to October at Gangwon province, and well-selected seeds were used while being dry-stored at 4±1℃. The seed size ranged 4.52 × 1.58 ㎜ and the weight of thousand seeds were 1,603.5 ± 0.02 ㎎. The moisture content was 7.2%. Seeds were achene type, and morphology characters showed an elliptical shape and rough texture, and light brown in color. Moist-chilling treatment was conducted for dormancy breaking because the seeds had an undeveloped embryo of liner type. The embryo had developed during a moist-chilling period, constantly, and fully developed in 10 weeks. Consequently, it seemed to be non-deep complex or intermediate complex type of morphophysiological dormancy, and embryo dormancy was broken by wet-chilling for 10 weeks. After 10 weeks of wet-chilling treatment, seed germination began. Germination percentage was higher in dark condition raher than light condition and recorded the maximum at 25℃ in the dark (16.3%). A pre-soaking treatment with a combined plant growth hormones promoted germination and shortened T₅₀. Specifically, seed germination of 84.5% was achieved by pre-soaking of seeds with a combined solution of 500 ㎎/L GA₃ and 10 ㎎/L kinetin for 24 h after a wet-chilling treatment for 10 weeks. Thus the effect of plant growth hormones coupled with chilling temperature on seed breaking dormancy provide asubsequent growth of seedlings for successful plantation.

• Korean Journal of Plant Taxonomy
• /
• v.48 no.4
• /
• pp.331-356
• /
• 2018

We investigated the flora of Jogyesan Mt. and discussed important vascular plants found on this mountain. In total, 16 separate field trips (20 days overall) were completed from 2013 to 2017. There are 577 taxa on Mt. Jogyesan, consisting of 115 families, 333 genera, 520 species, 8 subspecies, 45 varieties, and 4 forms. Eighteen endemic plants of Korea were found on Jogyesan Mt., including Carex okamotoi Ohwi, Coreanomecon hylomeconoides Nakai, and Stewartia koreana Nakai ex Rehder. There are 80 taxa of floristic regional indicator plants in total, including level V such as Adenophora palustris Kom. and level IV such as Dryopteris championii (Benth.) C. Chr. ex Ching, Gastrodia elata Blume, Lindera sericea (Siebold & Zucc.) Blume and Corylopsis glabrescens var. gotoana (Makino) T. Yamanaka. There also are 24 taxa of naturalized plants, including Ambrosia artemisiifolia L.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.21 no.6
• /
• pp.27-53
• /
• 2018

This study was carried out to investigate the flora of Manisan (Ganghwa-gun) from April of 2017 to August of 2018. The vascular plants identified during the 12 round field surveys were a to total of 508 taxa: 107 families, 306 genera, 456 species, 7 subspecies, 43 varieties, 1 form, and 2 hybrids. The largest families were as follows; Asteraceae (66 taxa), Poaceae (59 taxa), Fabaceae (25 taxa), Cyperaceae (25 taxa), Rosaceae (22 taxa), Liliaceae (20 taxa), Polygonaceae (17 taxa), Lamiaceae (14 taxa), Ranunculaceae (13 taxa), and Caryophyllaceae (13 taxa). Among these plants, 169 taxa were recoeded for the first time from this region. The six taxa of Korean endemic plants including Clematis brachyura Maxim., Viola seoulensis Nakai, Carex brevispicula G. H. Nam & G. Y. Chung, C. erythrobasis H. $L{\acute{e}}v$. & Vaniot, Hemerocallis hakuunensis Nakai, and Lycoris flavescens M. Y. Kim & S. T. Lee were found in this region. The vascular plants on the red list according to IUCN evaluation basis were found to be two taxa: Near Threatened (NT) species of Wikstroemia trichotoma (Thunb.) Makino, and Not Evaluate (NE) species of Tylophora floribunda Miq., respectively. The floristic regional indicator plants found in this area were 26 taxa comprising three taxa of degree IV, four taxa of degree III, seven taxa of degree II, and 12 taxa of degree I. In addition, the alien plants were identified as 49 taxa and the percentage of naturalized index (NI) was 9.7%, and urbanization index (UI) was 15.3%, respectively.

• Journal of Bio-Environment Control
• /
• v.22 no.2
• /
• pp.131-137
• /
• 2013

This study was conducted to develop new indoor plants and to investigate the effects of light intensity on the growth of Trachelospermum asiaticum var. intermedium Nakai and Piper kauzura. Most of T. asiaticum var. intermedium Nakai under 100 lux light intensity were blighted in two months, whereas survival rate was 83.3% after five months under 1,000 lux and 2,500 lux. Plant height, number of leaf, leaf width and leaf length increased as light intensity increased. The plants maintained under 2,500 lux showed the greatest plant height and leaf number. Piper kadzura Ohwi was defoliated and blighted in four months under 100 lux light intensity, whereas survival rate was 100% after five months under 1,000 lux and 2,500 lux. But, 1,000 lux light intensity was more favorable chlorophyll contents, leaf length and leaf width than 2,500 lux light intensity. Slight increase of chlorophyll contents and leaf growth under 1,000 lux indicates that Piper kadzura Ohwi could be utilized highly as a indoor ornamental plant.

• Asian-Australasian Journal of Animal Sciences
• /
• v.7 no.4
• /
• pp.509-512
• /
• 1994

High performance liquid chromatography (HPLC) analysis was applied for determination of guanine plus cytosine (G + C) contents of DNA of Butyrivibrio fibrisolvens. By values of G + C contents, a reference strain and 20 wild strains of B. fibrisolvens were classified into at least two distinct subgroups, i.e. G + C contents of 18 strains were 38-40 mol% and those of 3 strains including the reference strain were 43-45 mol%. Clear relationships were not observed between G + C contents and biological properties of 21 strains of B. Fibrisolvens.

• The Korean Journal of Food And Nutrition
• /
• v.3 no.2
• /
• pp.177-184
• /
• 1990

General components, amino acids and minerals contained in T kind of edible wild grasses were estimated. Samples were collected from the field of Kapyong area, Kyung-ki, Korea. Results of the estimation were summarized as follows ; 1) A relatively large amounts of protein and fat were contained in the edible parts of each sample. 2) The most abundant amino acids in the edible parts of each sample were glutamic acid (26.6%), serine(18.7%), valise(22.4%), respectively. 3) A high level of calcium and iron was found in the edible parts of each samples. Especially calcium was abundant in the leaves of Rumex coreanus Nakai and iron was abundant in the Commelina communis L. Both copper and zinc were abundant in the Sanguisorba officinalis L.

• Journal of Plant Biology
• /
• v.18 no.4
• /
• pp.143-149
• /
• 1975

For the evaluation of drought resistance of the plant, the growth of Lespedeza bicolor Turcz. var. japonica Nakai was analyzed by the control of water content of soil: 1. The growth of leaf, stem and root showed high value in accordance with the increase of soil water content. 2. The formation of nodule was alos increased as the content of soil water became higher. 3. The highest water content of the plant was shown in the plots of 30 and 40% of water centent in soils and in the middle of the growing period (August-September). 4. The C/F ratio in the early period of the growth(July) was similar in each plot, but showed a higher value as the water centent of soil became higher at the later period(October). 5. The T/R ration increased in early period of the growth as the soil water content became higher, but it was decreased to the value of 1 in each plot.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.1
• /
• pp.60-64
• /
• 2016

Scopolia parviflora of the family Solanaceae is an endemic species of Korea and a traditional Korean medicinal plant. The plastid genome was sequenced by next-generation sequencing (NGS) method. The characterized cp genome is 156,193 bp in size; the large single-copy (LSC) region is 86,364 bp, the inverted repeat (IR) is 25,905 bp, and the small single copy (SSC) region is 18,019 bp. The overall GC content of the plastid genome amounts to 37.61%. The cp genome contains 113 genes and 21 introns, including 80 proteincoding genes, four RNA genes, 30 tRNA genes, 20 group II introns, and one group I intron. A phylogenetic analysis showed that Scopolia parviflora was closely related to Hyoscyamus niger.

• Publications of The Korean Astronomical Society
• /
• v.30 no.2
• /
• pp.649-650
• /
• 2015

We have been performing daily VLBI monitoring of the flux density of Sagittarius (Sgr) $A^{\ast}$ at 22 GHz from February 2013 to August 2014 using a sub-array of the Japanese VLBI Network (JVN). The purpose of this monitoring is to explore the flux density variability at daily time resolution for a period longer than one year with the G2 cloud approaching. The flux density of Sgr $A^{\ast}$ is basically stable during the observational period, though there are some small variations. The average and scattering range are consistent with the previously observed values. We have observed no strong flare of Sgr $A^{\ast}$ although it is near the expected peri-center passing.