• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.12
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• pp.1309-1316
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• 2011

This paper presents a high-speed RNA microextractor for the direct isolation of RNA from blood lysate using magnetic oligo(dT) beads. The extraction is performed through lateral magnetophoresis, which is induced by a ferromagnetic wire array inlaid. With this RNA microextractor, more than 80% of the magnetic beads could be separated at a flow rate up to 20 ml/h, and the overall extraction procedure was completed within 1 min. The absorbance ratio of RNA to protein(A260/A280) was greater than 1.7, indicating that the extraction technique yields pure RNA. The feasibility of using this technique in reverse transcription polymerase chain reaction procedures was investigated by cDNA synthesis and PCR processes. The results confirmed that the RNA microextractor is a practical device for easy, fast, and high-precision RT-PCR using minimal amounts of reagent.

• Journal of Korean Society of Steel Construction
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• v.14 no.3
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• pp.403-411
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• 2002

This paper described the ultimate strength and deformation limit of welded T-joints in cold-formed square hollow sections. Previous studies showed that the T-joint has an obvious peak load. with the failure mode of chord-web buckling at a ratio of branch width to chord width ($\beta$) of above 0.8. Similar to a T-joint with chord-flange failure mode, the strength at a certain deformation limit can be regarded as the ultimate strength of a T-joint Based on the experimental results including tests done by Kato and Zhao, the deformation limit was proposed as 3%B for $10.7{\leq}2{\gamma}(B/T){\leq}42.3$ and $0.8{\leq}{\beta}{\leq}1.0$. The strength formula of CIDECT and those of other researchers were also compared with the test results. Finally, the strength formula based on the column buckling was proposed.

• International Area Studies Review
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• v.13 no.3
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• pp.527-549
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• 2009

This paper investigates the relation of invert U-shape between the M/B ratio and leverage ratio by market, firm size, and a level of technology of firm. Our sample consists of 510 manufacturing firms continually listed on the Korea Securities Market and Kosdaq Market from January 1, 2001 to December 31, 2008. The total sample consists of the Korea Securities Market(large firms, high technology industry) sample of 2,248(1,816, 1,704) observations and the Kosdaq Market(small and medium firms, high technology industry) sample of 1,632(2,264, 2,376) observations. The empirical results show that the relation of invert U shape appears on the sample of the Kosdaq Market, small and medium firms, and high technology industry. However, the relation doesn't appear on the sample of the Korea Securities Market, large firms, and low technology industry. These mutually different results may be caused by the relatively low M/B ratio of the latter.

• Bulletin of the Society of Naval Architects of Korea
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• v.11 no.2
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• pp.15-22
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• 1974

As for the added mass moment of inertia of ships in torsional vibration, it seems that the works by T. Kumai[1,2] are the only systematic one available currently. The work[1] is for the calculation of the two dimensional correction factors with finitely-long elliptic cylinders as the mathematic model. In this work the authors recalculated the above factors, $J_{\tau}$, with the same mathematic model and the same problem formulation, and presented the numerical results in Fig. 1. The reason why the reinvestigation was done was that in Kumai's work he obtained the solutions of the Mathieu equations, which was derived from the problem formulation for the velocity potential, under the assumption that the dummy constant q involved in the equations was always far less than unity, whereas in fact it takes values within the region of $0<q{\leq}{\infty}$ in sequence. As a result the authors found two remarkable differences in general features of $J_{\tau}$(refer to Fg.3); one that the authors' numerical results are considerably higher than the results given in [2], and the other that for a given number of node those have properties of decreasing monotonically with increase of the beam-draft ratio while these rapidly decrease from a maximum value of near at B/T=2.00 with B/T becoming greater or less than ratio. It seems that the latter trend was resulted from the fact that the assumption of $q{\ll}1$ employed in [2] was more closely satisfied in the vicinity of B/T=2.00.

• Journal of Korean Society of Steel Construction
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• v.21 no.5
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• pp.537-544
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• 2009

The design standards, such as AISC-LRFD (2005) and KBC-2005, specify the maximum width-to-thickness ratio that can be used for computing the strength of the concrete-filled tube (CFT), and do not include any formula for computing the strength when the width-to-thickness ratio is over the limit. This paper proposes a strength equation for CFTs with a large width-to-thickness ratio by acknowledging the fact that the stiffened slender steel platehas substantial postbuckling strength, and that it therefore can be more economical to use it. The equation adopts the concept of effective width,which is very useful for plate analysis. By comparing the strengths of AISC2005, KBC2005, and the proposed method with the results of the experiment, where the width-to-thickness ratio was regarded as the main parameter, the applicability of the proposed method was verified.

• The Korean Journal of Nuclear Medicine
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• v.38 no.6
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• pp.492-497
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• 2004

Purpose: it is very important to differentiate breast cancer from benign mass. There are many reports to evaluate the differential diagnosis under the several diagnostic tools. We evaluated the usefulness of mammography and Tc-99m MIBI scintimammography in the differential diagnosis of breast mass and correlated with pathologic findings. Materials and Methods: This study included 80 patients (a8e: 24-72, mean: 48.4) who underwent mammography and Tc-99m MIBI scintimammography for breast masses. Scintimammographies (anterior-posterior and lateral projections) were acquired in 10 minutes and 2 hours after intravenous injection of Tc-99m MIBI. four specialists in diagnostic radioloay and nuclear medicine evaluated the findings of breast masses under the mammography and Tc-99m MIBI scintimammography, and calculated the tumor to background (T/B) ratio. The pathologic results were obtained and we statistically analyzed the correlations between pathologic results and imaging findings under the mammography and Tc-99m MIBI scintimammography by chi-square and correlation test. Results: The sensitivity, specificity, positive predictive value, and negative predictive value of mammography for detection of breast cancer were 87.5%, 56.3%, 75.0%), and 75.0% respectively. 45 cases of 80 patients were suspicious for breast cancer under the Tc-99m MIBI scintimammography. 41 cases of 45 patients were confirmed as breast cancer and the remaining 4 cases were confirmed as benign masses. The sensitivity, specificity, positive predictive value and negative predictive value of Tc-99m MIBI scintimammography for detection of breast cancer were 85.4%, 87.5%, 91.1%, and 80.8% respectively. The sensitivity of scintimammography was lower than that of mammography for detection of breast cancer, however the specificity, positive predictive value, and negative predictive value were higher. In the benign mass, the mean T/B ratio in 10 minutes was $1.409{\pm}0.30$, and that in 2 hours was $1.267{\pm}0.42$. The maximal T/B ratio of benign mass in 10 minutes was $1.604{\pm}0.42$, and that in 2 hours was $1.476{\pm}0.50$. In the malignant mass, the mean T/B ratio in 10 minutes was $2.220{\pm}1.07$, and that in 2 hours was $1.842{\pm}0.75$. The maximal T/B ratio of malignant mass was $2.993{\pm}1.94$, and that in 2 hours was $2.480{\pm}1.34$. And the T/B ratio under the early and delayed images were meaningful. Conclusion: The scintimammography is useful diagnostic tool to differentiate breast cancer from benign mass, although the sensitivity of mammography for detection of breast mass is high. Especially, the use of the T/B ratio is helpful to diagnose breast cancer.

• Journal of Advanced Marine Engineering and Technology
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• v.24 no.6
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• pp.24-33
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• 2000

This paper investigates the relationship between J-integral and crack tip opening displacement, ${\delta}_t$ using Gordens results of numerical analysis. Estimation were carried out for several strength levels such as ultimate, flow, yield, ultimate-flow, flow-yield stress to determine the influence of strain hardening and the ratio of crack length to width on the $J-{\delta}_t$ relationship. It was found that for SE(B) specimens, the $J-{\delta}_t$ relationship can be applied to relate J to ${\delta}_t$ as follows $J=m_j{\times}{\sigma}_i{\times}{\delta}_t$ where $m_j=1.27773+0.8307({\alpha}/W)$, ${\sigma}_i:{\sigma}_U$, ${\sigma}_{U-F}={\frac{1}{2}} ({\sigma}_U+{\sigma}_F$), ${\sigma}_F$, ${\sigma}_F}$ $Y=({\sigma}_F+{\sigma}_Y)$, ${\sigma}_Y$

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.6 no.4
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• pp.354-364
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• 1994

Experiments have carried out to investigate the effects of parameters, such as mass ratio of R141b-to-water, stirrer speed, brine inlet temperature, brine flowrate, and additives, on the performance of the cool storage system using R141b clathrate. The cool storage system in this experiment was composed of storage tank, refrigerator, and heater. The results show that the mass ratio of R141b-to-water, 1 : 3~1 : 3.5 gives the best performance and the stirring speed has optimum point as 600rpm. At this speed impeller Reynolds number is $1.01{\times}10^5$. The lower the inlet brine temperature and the highter the brine flowrate, the better performance. The addition of metal powder turned out to reduce the degree of supercooling. The supercooling reduction was proportional to the amount of the metal power. However when metal powder was added more than 0.1 wt%, there was no additional supercooling reduction. The surfactants shortened the time consumed for cool storage to the half of no surfactant added case.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.10a
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• pp.433-436
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• 2009

This study is concerned with an effect of frictional condition in a forward/backward combined extrusion process. Generally, the material flow of the billet is influenced by the corners of the die cavity, the ratio in reduction in area, and thickness ratio of backward can thickness to forward can thickness. In addition, the frictional condition in contact area between the billet and the punch/die also affect the material flow. This paper investigated the effect of frictional condition for variable friction factors. The FEM simulation has been carried out in order to examine the effect of frictional condition. Deformation patterns and flow characteristics were examined in terms of design parameters such as extruded length ratio etc. Die pressure exerted on the die-workpiece interface is calculated by the simulation results and analyzed for safe tooling. Therefore the numerical simulation works provide a combined extrusion process of stable cold forging process planning to avoid the severe damage on the tool.

• Transactions of Materials Processing
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• v.18 no.7
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• pp.550-555
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• 2009

In the multi-pass shape drawing process, the pass schedule that includes the determination of reduction ratio and intermediate die shape is very important. This study used the equal reduction, equal load, and electric field analysis method for pass schedule of the multi-pass shape drawing. The reduction ratio was calculated by the equal reduction and equal load method. And the intermediate die shape was determined by the result of the electric field analysis and the calculated reduction ratio. The proposed pass schedule method was applied to a shape drawing for producing cross roller guide. Finally, FE-analysis and shape drawing experiment were performed to verify the effectiveness of the proposed method.