• KIEAE Journal
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• v.11 no.4
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• pp.63-70
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• 2011

In respond to the global energy crisis and climate change, there have been many ongoing national efforts to develop a sustainable housing prototype followed by "2 million Green Home Project" in Korea. More than 50% of nation's population are currently living in apartment housing thus the country is seriously in need of developing green apartment prototype. In this research, we focused on energy-conservative green apartment design prototype that have both passive components and active systems explored in a systemic design approach. After selecting an existing basic apartment unit, we analyzed and compared statistical data with the simulated annual energy consumption to match these two data sets for validating simulation accuracy performed with TRNSYS package. We performed energy simulations with different passive design factors such as varied insulation thickness, window types and infiltration rates as well as the active design factors including boilers and lighting fixtures to analyze their impacts on the energy performance of the housing unit using TRNSYS software. As a result, we acquired significant energy reduction effect with explored design strategies but the life cycle cost analysis for the final design guidline would need to be performed. In this study, we focused on a systematic comparative energy analysis based on TRNSYS that can improve the design of a green apartment housing.

• Journal of Life Science
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• v.11 no.4
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• pp.385-391
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• 2001

Filamentous hemagglutinin (FHA) is considered as an essential immunogenic component for incorporation into acellular vaccines against Bordetella pertussis, the causative agent of whooping cough. Classically, antipertussis vaccination has employed an intramuscular route. An alternative approach to stimulate mucosal and systemic immune responses is oral immunization with recombinant live vaccine carrier strains of Salmonella typhimurium. An attenuated live Salmonella vaccine sgrain($\Delta$cya $\Delta$crp) expressing recombinant FHA(rFHA) was developed. Stable expressionof rFHA was achieved by the use of balanced-lethal vector-host system. which employs an asd deletion in the host chromosome to impose in obligate requirement for diaminopimelic acid. The chromosomal $\Delta$asd mutation was complemented by a plasmid vector possessing the asd$^{+}$ gene. A 3 kb DNA fragment encoding immuno dominant regionof FHA was subcloned in-frame downstream to the ATG translation initiation codon in the multicopy Asd$^{+}$ pYA3341 vector to create pYA3457. Salmonella vaccine harboring pYA3457 expressed approximately 105kDa rFHA protein. The 100% maintenance of [YA3457 in vaccine strain was confirmed by stability examinations. Additionally, a recombinant plasmid pYA3458 was constructed to overpress His(8X)-tagged rFHA in Essherichia coli. His-tagged rFHA was purified from the E. coli strain harboring pYA3458 using Ni$^{2+}$-NTA affinity purification system.>$^{2+}$-NTA affinity purification system.

• BMB Reports
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• v.48 no.3
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• pp.147-152
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• 2015

Small interfering RNA (siRNA) functions through pairing with specific mRNA sequences and results in the mRNA's degradation. It is a potential therapeutic approach for many diseases caused by altered gene expression. The delivery of siRNA is still a major problem due to its rapid degradation in the circulation. Various strategies have been proposed to help with the cellular uptake of siRNA and short or small hairpin RNA (shRNA). Here, we reviewed recently published data regarding local applications of siRNA. Compared with systemic delivery methods, local delivery of siRNA/shRNA has many advantages, such as targeting the specific tissues or organs, mimicking a gene knockout effect, or developing certain diseases models. The eye, brain, and tumor tissues are 'hot' target tissues/organs for local siRNA delivery. The siRNA can be delivered locally, in naked form, with chemical modifications, or in formulations with viral or non-viral vectors, such as liposomes and nanoparticles. This review provides a comprehensive overview of RNAi local administration and potential future applications in clinical treatment.

• Korean System Dynamics Review
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• v.7 no.2
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• pp.191-213
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• 2006

Increasing of the number of Roe Deer in Jesudo is regarded as an direct cause of the damages of Roe Deer - i.e. damages of crops and trees by Roe Deer and traffic accidents between human and Roe Deer. But, no study of the number of Roe Deer in Jejudo has been found and never has been progressed it and nobody convinces about the total number of Roe Deer in Jejudo. In this paper, we focus at the moving pressure which enforces Roe Deer to move the other places. Moving pressure is appeared when the habitat environment destruction of Roe Deer and it forces Roe Deer in hilly section to move the middle mountains section and the low section. This moving pressure promotes the moving of Roe Deer to the other places and then the total number of Roe Deer in new places are increasing. High density of Roe Deer makes the habitat environment bad and increases the competition of Roe Deer. These patterns are repeated continuously. The habitat environment of Roe Deer is related with human life area very closely. We should keep and preserve our nature and environment, and if we develop our nature then we must consider our ecosystem in all aspects. It's the most important thing to us and Roe Deer and other living things.

• Journal of Korean Neurosurgical Society
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• v.42 no.2
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• pp.149-152
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• 2007

Authors report a very rare case of primary Hodgkin's lymphoma limited to the cerebellum. A 64-year-old female patient presented with headache, nausea, and vomiting. Magnetic resonance imaging of the brain revealed a nodular enhancing mass in the left cerebellar hemisphere. Tumor was removed totally with retromastoid suboccipital approach. Diagnosis was Hodgkin's lymphoma of mixed cellularity type, consisting of a dense mixed inflammatory infiltrate containing scattered large atypical mononuclear Hodgkin's cell and multinucleated Reed-Sternberg cells. Systemic work-up for the Hodgkin's lymphoma was followed with negative result. Postoperative radiation therapy was given and the patient is disease-free at 16 months after the operation.

• Journal of Korean Neurosurgical Society
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• v.57 no.1
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• pp.50-53
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• 2015

Usually fungal infections caused by opportunistic and pathogenic fungi had been an important cause of morbidity and mortality among immunocompromised patients. However clinical data and investigations for immunocompetent pathogenic fungal infections had been rare and neglected into clinical studies. Especially Cryptococcal brainstem abscess cases mimicking brain tumors were also much more rare. So we report this unusual case. This 47-year-old man presented with a history of progressively worsening headache and nausea for 1 month and several days of vomituritions before admission. Neurological and laboratory examinations performed demonstrated no abnormal findings. Previously he was healthy and did not have any significant medical illnesses. A CT and MRI scan revealed enhancing $1.8{\times}1.7{\times}2.0$ cm mass lesion in the left pons having central necrosis and peripheral edema compressing the fourth ventricle. And also positron emission tomogram scan demonstrated a hot uptake of fluoro-deoxy-glucose on the brainstem lesion without any evidences of systemic metastasis. Gross total mass resection was achieved with lateral suboccipital approach with neuronavigation system. Postoperatively he recovered without any neurological deficits. Pathologic report confirmed Cryptococcus neoformans and he was successively treated with antifungal medications. This is a previously unreported rare case of brainstem Cryptococcal abscess mimicking brain tumors in immunocompetent host without having any apparent typical meningeal symptoms and signs with resultant good neurosurgical recovery.

• Journal of Korean Neurosurgical Society
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• v.48 no.1
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• pp.88-90
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• 2010

Retroperitoneal hematoma (RH) due to radiologic intervention for an intracranial lesion is relatively rare, difficult to diagnose, and can be lifethreatening. We report a case of RH that developed in a patient on anticoagulant therapy following endovascular coiling of a ruptured anterior communicating artery (AcoA) aneurysm. An 82-year-old man presented with a 12-day history of headache. Computed tomography (CT) on admission demonstrated slight subarachnoid hemorrhage, and left carotid angiography revealed an AcoA aneurysm. The next day, the aneurysm was occluded with coils via the femoral approach under general anesthesia. The patient received a bolus of 5,000 units of heparin immediately following the procedure, and an infusion rate of 10,000 units/day was initiated. The patient gradually became hypotensive 25 hours after coiling. Abdominal CT showed a huge, high-density soft-tissue mass filling the right side of the retroperitoneum space. The patient eventually died of multiple organ failure five days after coiling. RH after interventional radiology for neurological disease is relatively rare and can be difficult to diagnose if consciousness is disturbed. This case demonstrates the importance of performing routine physical examinations, sequentially measuring the hematocrit and closely monitoring systemic blood pressures following interventional radiologic procedures in patients with abnormal mental status.

• Korean Journal of Childcare and Education
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• v.16 no.5
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• pp.27-45
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• 2020

Objective: The purpose of this study is to develop a music making program that utilizes the characteristics of the picture books medium through the ADDIE model so that teachers can easily apply it to children, and to determine whether it affects children's musical aptitude and music creativity. Methods: The Participants of the study were 42 five-year-old children attending a daycare in Seoul, of which 20 were in the experimental group and 22 in the comparative group. The experimental group participated in a music production program using picture books, and during the same period, the comparison group participated in music activities suggested by the Nuri Course. Using the SPSS 22.0 program, average, standard deviation, independent sampling t-test, and ANCOVA(Analysis of Covariance) were calculated. Results: The music making program using picture books improved children's rhythm and tone which are the sub-items of music aptitude. The music making program enhanced children's music flexibility, music creativity, music logic which are the sub-items of music creativity. Conclusion/Implications: The music making program presented systemic teaching-learning method with which teachers explained the modeling and practiced from simple activities to various activities repeatedly in order to make teachers approach music making more easily. It is recommendable to make the music making program by using I-pad and computers.

• Child Health Nursing Research
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• v.9 no.2
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• pp.190-197
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• 2003

Purpose: This study is to identify the present situation of children's hospice and to find the developing strategies for child hospice system in Korea. Method: The data was collected from both literatures and the recent data provided by the government. The direction of future of children's hospice cared in Korea was predicted based on the literature analysis and the report and policy of government. Result: In Korea, the system of the child hospice is not processing. There are the importance differences between children and adult in that the characteristics and approach of the hospice care. All medical personnel and the people related to hospice care including children and their family should be recognized the necessity of the children's hospice care. The following strategies is needed for setting up the child hospice :the principles and standards, recognizing of the necessities, developing of educational program for the specialist and the systemic children's hospice program, and the organization of child hospice. Conclusion: Directions suggested from this study have the importance of child hospice to establish and develop well in Korea for both children with life-threatening disease and their families.

• Clinical and Experimental Pediatrics
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• v.54 no.5
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• pp.183-191
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• 2011

Although antenatal diagnostic technique has considerably improved, precise detection and proper management of the neonate with congenital heart disease (CHD) is always a great concern to pediatricians. Congenital cardiac malformations vary from benign to serious conditions such as complete transposition of the great arteries (TGA), critical pulmonary and aortic valvular stenosis/atresia, hypoplastic left heart syndrome (HLHS), obstructed total anomalous pulmonary venous return (TAPVR), which the baby needs immediate diagnosis and management for survival. Unfortunately, these life threatening heart diseases may not have obvious evidence early after birth, most of the clinical and physical findings are nonspecific and vague, which makes the diagnosis difficult. High index of suspicion and astute acumen are essential to decision making. When patent ductus arteriosus (PDA) is opened Widely, many serious malformations may not be noticed easily in the early life, but would progress as severe acidosis/shock/cyanosis or even death as PDA constricts after few hours to days. Ductus dependent congenital cardiac lesions can be divided into the ductus dependent systemic or pulmonary disease, but physiologically quite different from each other and treatment strategy has to be tailored to the clinical status and cardiac malformations. Inevitably early presentation is often regarded as a medical emergency. Differential diagnosis with inborn error metabolic disorders, neonatal sepsis, persistent pulmonary hypertension of the newborn (PPHN) and other pulmonary conditions are necessary. Urgent identification of the newborn at such high risk requires timely referral to a pediatric cardiologist, and timely intervention is the key in reducing mortality and morbidity. This following review deals with the clinical presentations, investigative modalities and approach to management of congenital cardiac malformations presenting in the early life.