• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.6
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• pp.397-404
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• 1991

The steady state and decay characteristics of primary fluorescenece of phytoplanktons including Cyanophyceae and Cryptophyceae were investigated in vivo. At 580-640 nm region, fluorescence emission spectra were obtained from all algae examined. The observed fluorescence emission maxima were similiar$(\pm3\;nm)$ except Synechocorcus sp. (SYN). Considered $\lambda_{max}$ of emission spectra of phycobiliproteins and the excitation spectra with $\lambda_{max}=540-560nm$, it seems to be originated from biliproteins. Fluorescence lifetimes $(\tau)$ and decay curves were compared with standard solution of candidate organic compounds, b-phycoerythrin. The $\tau$ values obtained for phytoplankton with $\lambda_{max}=580nm$ were different depending upon the species of algae. The observed $\tau$ values were ranged from 1.39 ns to 1.95 ns. These are considerably shorter than $\tau(3.23\;us)$ for standard solution of b-phycoerythrin. The reduction of $\tau$ for phycoerythrin in vivo seems to be originated from effective energy transfer system between Chl. a and phycobiliprotein in intact cell. There are subtantial differences in fluorsecence spectra and lifetimes at the class level. At the species level, differences seems to be much smaller. The result of experiment suggests that measurement of fluorescence lifetimes may be helpful in the rapid characterization of algae. Direct application will likely be found in combination with the measurement of other luminescence parameters.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.40-45
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• 2001

Raw starch-digesting amylase (BF-2A, M.W. 93, 000 Da) from Bacillus circulans F-2 was converted to two components during digestion with subtilisin. Two components were separated and designated as BF-2A' (63, 000 Da) and BF-2B (30, 000 Da), respectively. BF-2A' exhibited the same hydrolysis curve for soluble starch as the original amylase (BF-2A). Moreover, the catalytic activities of original and modified enzymes were indistinguishable in $K_{m}$, Vmax for, and in their specific activity for soluble starch hydrolysis. However, its adsorbability and digestibility on raw starch was greatly decreased. Furthermore, the enzymatic action pattern on soluble starch was greatly different from that of the BF-2A. A smaller peptide (BF-2B) showed adsorb ability onto raw starch. By these results, it is suggested that the larger peptide (BF-2A') has a region responsible for the expression of the enzyme activity to hydrolyze soluble substrate, and the smaller peptide (BF-2B) plays a role on raw starch adsorption. A similar phenomenon is observed during limited proteinase K, thermolysin, and endopeptidase Glu-C proteolysis of the enzyme. Fragments resulting from proteolysis were characterized by immunoblotting with anti-RSDA. The proteolytic patterns resulting from proteinase K and subtilisin were the same, producing 63- and 30-kDa fragments. Similar patterns were obtained with endopeptidase Glu-C or thermolysin. All proteolytic digests contained a common, major 63-kDa fragment. Inactivation of RSDA activity results from splitting off the C-terminal domain. Hence, it seems probable that the protease sensitive locus is in a hinge region susceptible to cleavage. Extracellular enzymes immunoreactive toward anti-RSDA were detected through whole bacterial cultivation. Proteins of sizes 93-, 75-, 63-, 55-, 38-, and 31-kDa were immunologically identical to RSDA. Of these, the 75-kDa and 63-kDa proteins correspond to the major products of proteolysis with Glu-C and thermolysin. These results postulated that enzyme heterogeneity of the raw starch-hydrolysis system might arise from the endogeneous proteolytic activity of the bacterium. Truncated forms of rsda, in which the gene sequence encoding the conserved domain had been deleted, directed the synthesis of a functional amylase that did not bind to raw starch. This indicates that the conserved region of RSDA constitutes a raw starch-binding domain, which is distinct from the active centre. The possible role of this substrate-binding region is discussed.d.

• Journal of the Korean Wood Science and Technology
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• v.39 no.3
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• pp.230-237
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• 2011

The study was performed to investigate the characteristics of charcoal and temperature change of a kiln's inner and outer walls in carbonization process using improved kiln. In this kiln system, carbonization process was completed in eight days. In the kiln, the ignition temperature was kept about $720^{\circ}C$. And then the temperature were increased gradually prior to be refined. Finally, the temperature in refining process was reached to maximum point, $1,000^{\circ}C$. In the chimney, the temperature was increased gradually from $90^{\circ}C$ at ignition to $750^{\circ}C$ at refining. The temperature change of the kiln wall resembles a temperature change progress curve during a carbonization process. The highest temperature of the kiln wall that appeared by a carbonization process was around $500^{\circ}C$. As a result of having measured an inner wall and the outer wall of the kiln using an infrared thermography camera, it was judged with there being considerable latent heat on kiln wall and ceiling. Fixed carbon contented of charcoal was 85.9~89.9%. Refining degree of charcoal, hardness, calorific value and pH were l, 12, 7,047~7,456 kcal/kg, 9.0~9.9, respectively. The yield of wood charcoal was 13.8%, and compared to conventional kiln's yield increased 1.5%.

• Health Policy and Management
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• v.5 no.1
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• pp.80-105
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• 1995

Assuming that we introduced integration of medical insurance society for self-employed, this study was conducted to examine effects and results after the integration and to research more effective method for integration. To assess effects and results of the finacial status of 266 insurance societies after intergration, the data were obtained from "The Medical Insurance Program for Self-Employeds Statistical Yearbook in 1992". The major finding are as follows : 1. Three alternative integration proposals were made. First alternative proposal was consisted of 232 medical insurance societies, second was 187, and third was 115. 2. As the results of average number of the insured per insurance societies of medical insurance program for self-employed every alternative proposal, first was 88, 119 persons, second was 108, 576, and third was 178, 967 from 76, 576 persons of present socienties. 3. It was true that the more average size of societies increased, the more average administration expenditure per 1, 000 insured reduced. 4. The average size of societies grew bigger, the rate of general expenditure to general revenue more improved. Also, the rate of benefits to contributions was changed for better. But if not to have had correct analysis and precise preparation for integration, effects and results of integration were always not optiized. 5. According to results of simple regression formulas, it was proved that the more the average size of societies was increased, the more result was advantaged. 6. The law of majority and the economy of scale were applied in this study, and it was necessary to analyze and assess effectiveness and efficiency of integration. Therefore, when the integration of medical insurance societies for self-employeds will be performed, it must be taken into consideration. Among three alternative proposals, third was showed more effective alternative than anothe, second was presented more ineffective result than present system. To achieve more effective and efficient integration of regional medical insurance societies throughout the result of the regression formula on present cost curve, it is necessary to operate well-integrated societies and to know appropriative countermeasures of present situation of each societies. Also, for integrating regional medical insurance societies, it is necessary to continue more deep research through practical model activity and to investigate the effective size and managed method of the societies.societies.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.2
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• pp.228-232
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• 2018

Process-based crop models have been used to assess the impact of climate change on crop production. These models are implemented in procedural or object oriented computer programming languages including FORTRAN, C++, Delphi, Java, which have a stiff learning curve. The requirement for a high level of computer programming is one of barriers for efforts to develop and improve crop models based on biophysical process. In this study, we attempted to develop a Chinese cabbage model using Microsoft Excel with Visual Basic for Application (VBA), which would be easy enough for most agricultural scientists to develop a simple model for crop growth simulation. Results from Soil-Plant-Atmosphere-Research (SPAR) experiments under six temperature conditions were used to determine parameters of the Chinese cabbage model. During a plant growing season in SPAR chambers, numbers of leaves, leaf areas, growth rate of plants were measured six times. Leaf photosynthesis was also measured using LI-6400 Potable Photosynthesis System. Farquhar, von Caemmerer, and Berry (FvCB) model was used to simulate a leaf-level photosynthesis process. A sun/shade model was used to scale up to canopy-level photosynthesis. An Excel add-in, which is a small VBA program to assist crop modeling, was used to implement a Chinese cabbage model under the environment of Excel organizing all of equations into a single set of crop model. The model was able to simulate hourly changes in photosynthesis, growth rate, and other physiological variables using meteorological input data. Estimates and measurements of dry weight obtained from six SPAR chambers were linearly related ($R^2=0.985$). This result indicated that the Excel/VBA can be widely used for many crop scientists to develop crop models.

• Journal of Korea Water Resources Association
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• v.40 no.6 s.179
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• pp.459-468
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• 2007

To estimate the groundwater recharge, the fully distributed parameter based model, MIKE SHE was applied to the Gyeongan-cheon watershed which is one of the tributaries of Han River Basin, and covers approximately $260km^2$ with about 49 km main stream length. To set up the model, spatial data such as topography, land use, soil, and meteorological data were compiled, and grid size of 200m was applied considering computer ability and reliability of the results. The model was calibrated and validated using a split sample procedure against 4-year daily stream flows at the outlet of the watershed. Statistical criteria for the calibration and validation results indicated a good agreement between the simulated and observed stream flows. The annual recharges calculated from the model were compared with the values from the conventional groundwater recession curve method, and the simulated groundwater levels were compared with the observed values. As a result, it was concluded that the model could reasonably simulate the groundwater level and recharge, and could be a useful tool for estimating spatially/temporally the groundwater recharges, and enhancing the analysis of the watershed water cycle.

• Korean Journal of Materials Research
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• v.23 no.12
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• pp.714-721
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• 2013

A stoichiometric mixture of evaporating materials for $ZnAl_2Se_4$ single-crystal thin films was prepared in a horizontal electric furnace. These $ZnAl_2Se_4$ polycrystals had a defect chalcopyrite structure, and its lattice constants were $a_0=5.5563{\AA}$ and $c_0=10.8897{\AA}$.To obtain a single-crystal thin film, mixed $ZnAl_2Se_4$ crystal was deposited on the thoroughly etched semi-insulating GaAs(100) substrate by a hot wall epitaxy (HWE) system. The source and the substrate temperatures were $620^{\circ}C$ and $400^{\circ}C$, respectively. The crystalline structure of the single-crystal thin film was investigated by using a double crystal X-ray rocking curve and X-ray diffraction ${\omega}-2{\theta}$ scans. The carrier density and mobility of the $ZnAl_2Se_4$ single-crystal thin film were $8.23{\times}10^{16}cm^{-3}$ and $287m^2/vs$ at 293 K, respectively. To identify the band gap energy, the optical absorption spectra of the $ZnAl_2Se_4$ single-crystal thin film was investigated in the temperature region of 10-293 K. The temperature dependence of the direct optical energy gap is well presented by Varshni's relation: $E_g(T)=E_g(0)-({\alpha}T^2/T+{\beta})$. The constants of Varshni's equation had the values of $E_g(0)=3.5269eV$, ${\alpha}=2.03{\times}10^{-3}eV/K$ and ${\beta}=501.9K$ for the $ZnAl_2Se_4$ single-crystal thin film. The crystal field and the spin-orbit splitting energies for the valence band of the $ZnAl_2Se_4$ were estimated to be 109.5 meV and 124.6 meV, respectively, by means of the photocurrent spectra and the Hopfield quasicubic model. These results indicate that splitting of the ${\Delta}so$ definitely exists in the ${\Gamma}_5$ states of the valence band of the $ZnAl_2Se_4/GaAs$ epilayer. The three photocurrent peaks observed at 10 K are ascribed to the $A_1$-, $B_1$-exciton for n = 1 and $C_{21}$-exciton peaks for n = 21.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1854-1858
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• 2007

수자원 분야에서 가장 기본적이면서 중요한 과업 중의 하나는 고품질의 유량측정 자료를 확보하여 신뢰성 있는 수위-유량관계곡선식을 개발하는 것이다. 이는 수공구조물 설계, 친수 하천공간 조성, 친환경적인 하천의 설계, 하천 관리수량 산정, 홍수 예 경보 운영 등에 기본적인 자료를 제공하게 된다. 신뢰성 있는 곡선식은 계측장비의 개량과 유량관측 기준의 강화 등을 통하여 축적된 양질의 유량측정 자료로부터 개발될 수 있으며, 또한 수위관측소 지점의 하도특성과 통제구조물의 특성 등을 고려하는 것도 곡선식의 신뢰도를 높일 수 있다. 본 연구는 통제단면의 가장 낮은 부분의 수위로 정의되는 흐름이 0인 수위인 GZF(Gauge Height of Zero Flow) 평가에 관한 연구이다. 이와 같은 연구를 수행하기 위해서 GZF의 변화에 따라 곡선식의 신뢰도를 분석할 수 있는 시스템을 개발하였으며, 이 시스템은 사용자들이 쉽게 이용할 수 있는 엑셀 VBA(Visual Basic for Applications)를 이용하여 개발하였다. GZF 평가 시스템은 입력자료 구축 모듈, 수위관측소 지점의 하도 단면 입력 모듈, GZF 설정 모듈, GZF 평가 모듈의 4개 모듈로 구성되었다. 입력자료 구축 모듈은 기 개발된 곡선식의 GZF 적정성을 파악할 수 있도록 자료를 구축하는 모듈이며, 하도 단면 입력 모듈은 수위관측소 지점의 하상의 변화 유무와 구간분리, 기간분리 등의 필요성을 파악할 수 있도록 구성하였다. GZF 설정 모듈은 GZF의 변화가 곡선식의 신뢰도를 파악할 수 있도록 구성되었다. 마지막으로 GZF 평가 모듈은 기 개발된 곡선식의 GZF와 금회 개발될 곡선식의 GZF를 비교 분석할 수 있도록 구성되었다. 본 연구의 성과는 향후 수위-유량관계곡선식을 개발할 때 GZF 산정의 오류를 감소시켜 앞으로 개발될 곡선식의 신뢰도 향상에 기여를 할 것으로 판단된다.소를 파악해야한다. 7. 부아 유대에 대한 위협요소 확인을 위한 도구개발과 그들에 대한 효과적인 간호전략이 필요 된다. 8. 가족에 있어서 모든 부모행위가 하나의 독립변수로서 연구되어야 하고 부아유대 증진에 관한 연구가 시도되어야겠다. 오늘날 부모들은 임신기간동안 많은 정보에 접하기를 원한다. 산전, 산후의 교육과 지식은 긍정적인 부아 관계를 증진시키고, 이것은 아동의 발달에 크게 기여할 수 있다. 긍정적으로 이러한 관계는 가족단위를 강하게 통합시키게 되므로 건강관리자(Health care workers)들은 애착에 대해 높은 관심을 갖어야 하겠다.2유수지는 BTL사업을 통해 주변공단으로부터의 오폐수를 원천적으로 차단하도록 하였으며 2유수지를 매립하여 지하는 강우시 유출수 저류가 가능한 화물차주차장으로 활용하고 지상은 녹지공간으로 조성하여 공단근로자 및 지역주민을 위한 휴식공간으로 활용될 수 있도록 제안하였다. 본 연구는 남동유수지 환경 개선 사업 실행을 위한 정책 연구로 연구결과를 인천시가 적극 수용하기로 결정함에 따라 인천시의 환경 현안 문제인 남동유수지의 수질개선을 통해 시민의 휴식 및 여가선용 공간으로 활용하기 위한 사업의 기초자료로 활용되며 이미 설계검토가 시작되었다. 본 연구결과는 유수지 및 저수지의 환경개선 사업의 선두적인 성공사례로 국내 타 지역의 유사한 사업에 있어 벤치마킹을 할 수 있는 훌륭한 사례가 될 것이다.요 생산이 증가하자 군신의 변별(辨別)과 사치를 이유로 강력하게 규제하여 백자의 확대와 발전에 걸림돌이 되었다. 둘째, 동기(銅器)의 대체품으로 자기를 만들어 충당해야할 강제성 당위성 상실로 인한 자기수요 감소를 초래하였을 것으로 사료된다. 셋째, 경기도 광주에서 백자관요가 운영되었으므로 지방인 상주지역에도 더 이상 백자를 조달받을 필요가 없이, 일반 지방관아와 서민들의 일상용기 생산으로 전락하여 소규모화 되었을 것이라고 사료

• Journal of Broadcast Engineering
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• v.7 no.1
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• pp.45-54
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• 2002

This paper proposes a video compression algorithm using characteristics of wavelet coefficients. The proposed algorithm can provide lowed bit rate and faster running time while guaranteeing the reconstructed image qualify by the human virtual system. In this approach, each video sequence is decomposed into a pyramid structure of subimages with various resolution to use multiresolution capability of discrete wavelet transform. Then similarities between two neighboring frames are obtained from a low-frequency subband which Includes an important information of an image and motion informations are extracted from the similarity criteria. Four legion selection filters are designed according to the similarity criteria and compression processes are carried out by encoding the coefficients In preservation legions and replacement regions of high-frequency subbands. Region selection filters classify the high-frequency subbands Into preservation regions and replacement regions based on the similarity criteria and the coefficients In replacement regions are replaced by that of a reference frame or reduced to zero according to block-based similarities between a reference frame and successive frames. Encoding is carried out by quantizing and arithmetic encoding the wavelet coefficients in preservation regions and replacement regions separately. A reference frame is updated at the bottom point If the curve of similarity rates looks like concave pattern. Simulation results show that the proposed algorithm provides high compression ratio with proper Image quality. It also outperforms the previous Milton's algorithm in an Image quality, compression ratio and running time, leading to compression ratio less than 0.2bpp. PSNR of 32 dB and running tome of 10ms for a standard video image of size 352${\times}$240 pixels.

• Journal of Pharmaceutical Investigation
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• v.40 no.2
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• pp.101-107
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• 2010

A rapid and sensitive reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of N-(-4-Chlorophenyl)-6-hydroxy-7-methoxy-2-chromanecarboxamide (KAL-1120), a novel anti-inflammation agent, in the rat plasma. The method was applied to analyze the compound in the biological fluids such as bile, urine and tissue homogenates. After liquid-liquid extraction, the compound was analyzed on an HPLC system with ultraviolet detection at 275 nm. HPLC was carried out using reversed-phase isocratic elution with a $C_{18}$ column, a mobile phase of a mixture of acetonitril (40 v/v%) at a flow rate of 1.0 mL/min. The chromatograms showed good resolution and sensitivity and no interference of plasma. The calibration curve for the drug in plasma was linear over the concentration range of 0.05-50 ${\mu}g$/mL. The intra- and inter-day assay accuracies of this method ranged from 0.06% to 9.33% of normal values and the precision did not exceed 6.28% of relative standard deviation. The plasma concentration of KAL-1120 decreased to below the quantifiable limit at 1.5 hr after the i.v. bolus administration of 2-10 mg/kg to rats ($t_{1/2,({\alpha})}$ and $t_{1/2,({\beta})$ of 2.15 and 26.7 min at a dose of 2 mg/kg, 3.91 and 33.0 min at a dose of 10 mg/kg, respectively). The steady-state volume of distribution ($V_{dss}$) and the total body clearance ($CL_t$) were not significantly altered in rats given doses from 2 to 10 mg/kg. Of the various tissues tested, KAL-1120 was mainly distributed in the lung and heart after i.v. bolus administration. KAL-1120 was detected in the bile by 30 min after its i.v. bolus administration. However, the concentration in the urine after i.v. bolus administration became too low to measure, suggesting that KAL-1120 is mostly excreted in the bile. In conclusion, this analytical method was suitable for the preclinical pharmacokinetic studies of KAL-1120 in rats.