• IMMUNE NETWORK
• /
• 제4권3호
• /
• pp.131-142
• /
• 2004

In recent years, adjuvants have received much attention because of the development of purified subunit and synthetic vaccines which are poor immunogens and require adjuvants to evoke the immune response. Therefore, immunologic adjuvants have been developed and testing for most of this century. During the last years much progress has been made on development, isolation and chemical synthesis of alternative adjuvants such as derivatives of muramyl dipeptide, monophosphoryl lipid A, liposomes, QS-21, MF-59 and immunostimulating complexes (ISCOMS). Biodegradable polymer microspheres are being evaluated for targeting antigens on mucosal surfaces and for controlled release of vaccines with an aim to reduce the number of doses required for primary immunization. The most common adjuvants for human use today are aluminum hydroxide and aluminum phosphate. Calcium phosphate and oil emulsions have been also used in human vaccination. The biggest issue with the use of adjuvants for human vaccines is the toxicity and adverse side effects of most of the adjuvant formulations. Other problems with the development of adjuvants include restricted adjuvanticity of certain formulations to a few antigens, use of aluminum adjuvants as reference adjuvant preparations under suboptimal conditions, non-availability of reliable animal models, use of non-standard assays and biological differences between animal models and humans leading to the failure of promising formulations to show adjuvanticity in clinical trials. The availability of hundreds of different adjuvants has prompted a need for identifying rational standards for selection of adjuvant formulations based on safety and sound immunological principles for human vaccines. The aim of the present review is to put the recent findings into a broader perspective to facilitate the application of these adjuvants in general and experimental vaccinology.

• Bulletin of the Korean Chemical Society
• /
• 제26권5호
• /
• pp.740-746
• /
• 2005

In this article, we report the tandem mass spectrometry investigations for the electron capture efficiencies of the protons belonging to the different locations (generations) in a poly(propylene imine) dendrimer with three layers of a repeat unit (named as the third generation dendrimer). The employed tandem mass spectrometry methods include SORI-CAD (sustained off-resonance irradiation collisional activation dissociation) and ECD(electron capture dissociation) mass spectrometry. We obtained SORI-CAD spectra for the dendrimer ions in the different charge states, ranging from 2+ to 4+. The analysis of fragmentation sites provides the information as to where the protons are distributed among various generations of the dendrimer. Based upon this, a new strategy to study the electron capture efficiencies of the protons is utilized to examine a new type of triplycharged ions by SORI-CAD, i.e., the 3+ ions generated from the charge reduction of the native 4+ ions by ECD: (M+4H)$^{4+}\;+\;e^-\;{\rightarrow}$ (M+4H)$^{3+\bullet}$ ${\rightarrow}\;({H^{\bullet}}_{ejected}$) + (M+3H)$^{3+}\;\rightarrow$ CAD. Interestingly, comparison of these four SORICAD spectra indicates that the proton distribution in the charge-reduced 3+ ions is very close to that in the native 4+ ions. It further suggests that in this synthetic polymer ($\sim$1.7 kDa) with an artificial architecture, the electron capture efficiencies of the protons are actually insensitive to where they are located in the molecule. This is somewhat contradictory to common expectations that the protons in the inner generations may not be well exposed to the incoming electron irradiation as much as the outer ones are, thus may be less efficient for electron capture. This finding may carry some implications for the case of medium sized peptide ions with similar masses, which are known to show no obvious site-specific fragmentations in ECD MS.

• 한국지구과학회지
• /
• 제37권5호
• /
• pp.277-285
• /
• 2016

We introduce a depth scaling strategy to improve the accuracy of frequency-domain elastic full waveform inversion (FWI) using the new pseudo-Hessian matrix for seismic data without low-frequency components. The depth scaling strategy is based on the fact that the damping factor in the Levenberg-Marquardt method controls the energy concentration in the gradient. In other words, a large damping factor makes the Levenberg-Marquardt method similar to the steepest-descent method, by which shallow structures are mainly recovered. With a small damping factor, the Levenberg-Marquardt method becomes similar to the Gauss-Newton methods by which we can resolve deep structures as well as shallow structures. In our depth scaling strategy, a large damping factor is used in the early stage and then decreases automatically with the trend of error as the iteration goes on. With the depth scaling strategy, we can gradually move the parameter-searching region from shallow to deep parts. This flexible damping factor plays a role in retarding the model parameter update for shallow parts and mainly inverting deeper parts in the later stage of inversion. By doing so, we can improve deep parts in inversion results. The depth scaling strategy is applied to synthetic data without lowfrequency components for a modified version of the SEG/EAGE overthrust model. Numerical examples show that the flexible damping factor yields better results than the constant damping factor when reliable low-frequency components are missing.

• 한국응용과학기술학회지
• /
• 제33권2호
• /
• pp.333-341
• /
• 2016

산수유 종자 추출물의 천연 방부제로서 가능성을 평가하기 위하여 항균활성 및 화장품 방부 효능 평가를 실시하였다. 산수유 종자는 각각 1,3-부틸렌글리콜과 에탄올을 이용하여 추출하였다. Paper disc method로 항균활성을 평가한 결과 Staphylococcus aureus, Staphylococcus epidermidis와 Propionibacterium acnes에서 항균활성이 나타났으며 산수유 종자 1,3-부틸렌글리콜 추출물(COS-A)이 여드름균에대해 $26.7{\pm}4.0mm$로 가장 높았다. 최소저해농도(MIC) 측정 결과 산수유 종자 에탄올 추출물(COS-B)이 가장 낮은 농도에서 S. epidermidis균의 생장을 억제하였다. 에멀션에서의 방부효능 평가를 위해 Challenge test를 실시한 결과 COS-A와 COS-B를 첨가한 에멀션에서 균 접종 7일 후 피부상재균이 100% 사멸되었다. 이러한 결과는 산수유 종자 추출물이 화장품에서 합성방부제를 대체할 천연 방부제로서 가능성이 있을 것으로 사료된다.

• 대한가정학회지
• /
• 제44권3호
• /
• pp.27-49
• /
• 2006

This study investigated the integrated effects of family, school, internet, and mass culture factors on the identity formation of adolescents. Specifically, the purposes of this study were to categorize adolescents into groups by what they do during the time except the life in school and to investigate differences among the groups regarding the identity information(body image, self-esteem, self-identity) and the influences of environmental factors on identity formation of adolescent. The study distributed questionnaires to middle and high school adolescents of five representative cities in South Korea. The total respondents were 2240(960 from Seoul/Kyongki, and 320 each from Taegu, Pusan, Kwangju, and Taejon). The response rate was 98.7%. The data were analyzed by factor analysis, cluster analysis, ANOVA, Duncan test, and multiple regression. The results showed that Korean adolescents were segmented into five groups : TV preference group, computer preference group, friends and family involvement group, homework and study preference group, and study institute involvement group). The five groups were significantly different in regard to body image pursuit, self-esteem, and self-identity. The body image pursuit, self-esteem, and self-identity of adolescents were significantly different in regard to each environmental factors, in each adolescent group.

• 한국전자파학회논문지
• /
• 제17권2호
• /
• pp.184-192
• /
• 2006

개구 합성 레이더(SAR)는 지형의 고 해상도의 영상을 획득하는데 주로 사용된다. 본 논문은 X-band 주파수 대역의 차량 탑재형 개구 합성 레이더(automobile-based SAR) 시스템에 적용하기 위한 $16{\times}16$ 배열 안테나의 설계 및 제작에 관한 것이다. 본 논문에서 구현된 안테나는 레이돔, 방사체, 슬롯, 급전 구조 그리고 허니콤과 같은 여러 층의 구조로 되어 있으며 각 층들은 구조적인 그리고 전기적인 측면의 설계 요구 조건들을 만족하기 위하여 결합되어 있다. Strip-Slot-Foam-Inverted-Patch(SSFIP) 구조와 허니콤을 사용하여 넓은 동작 주파수 대역폭과 기구적인 안정성을 만족하였다. 측정 결과와 시뮬레이션 결과를 비교 분석하였으며 본 논문에서 설계한 안테나는 1.7 GHz의 동작 주파수 대역폭과 20 dB 미만의 side-lobe level, 각각 $5^{\circ}$의 빔 폭과 25 dBi의 이득을 가졌으며 측정된 결과는 광대역 SAR 시스템에 적용 가능할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권11호
• /
• pp.1562-1568
• /
• 2013

This study was conducted to evaluate the effects of indigenous herbal supplements on growth, blood metabolites and carcass characteristics in the late fattening period of Hanwoo steers. In a 6 month feeding trial, thirty Hanwoo steers ($647{\pm}32$ kg) were allotted to one of 5 treatment groups, control (basal diet contained lasalocid), licorice, clove, turmeric and silymarin, with six steers per pen. All groups received ad libitum concentrate and 1 kg rice straw/animal/d throughout the feeding trial. Blood samples were collected at the beginning, middle, and the end of the experiment and the steers were slaughtered at the end. Blood glucose, triglyceride, total protein, and albumin concentrations were higher in the turmeric treatment compared with other treatments. Blood urea nitrogen and creatinine concentrations were highest (p<0.003 and p = 0.071, respectively) in steers treated with silymarin. Alanine aminotransferase activity was lower (p<0.06) for licorice and silymarin compared with the control group. There were no alterations in serum aspartate aminotransferase and gamma glutamyltransferase activities as a consequence of herb treatments (p = 0.203 and 0.135, respectively). Final body weight, body weight gain, average dairy gain and dry matter intake were not significantly different among treatments. Yield grade, marbling score and quality grade were higher for silymarin group than those of the control group (p<0.05). Therefore, the results suggest that silymarin can be used an effective dietary supplement as an alternative to antibiotic feed additive and a productivity enhancer, providing safe and more consumer acceptable alternative to synthetic compounds during the late fattening period of steers.

• Korean Chemical Engineering Research
• /
• 제44권3호
• /
• pp.259-264
• /
• 2006

염화반응에 의한 $TaCl_5$의 제조에서 반응생성물 중 $NbCl_5$, $TiCl_4$, $FeCl_2$ 등이 주요 불순물로 존재하게 된다. $TaCl_5$와 $NbCl_5$는 증류나 수소 환원법에 의해 쉽게 분리가 되므로, 반응생성물에서 $TaCl_5/NbCl_5$ 혼합물을 99.9％ 이상 순도로 분리하기 위해 2기의 연속식 증류공정을 사용하여 light한 성분과 heavy한 성분을 제거하는 공정을 구성하였다. 본고에서는 순차배열(direct sequence)과 비 순차배열(indirect sequence)으로서의 두 분리공정에 대한 비교연구를 상용성 화학공정모사기인 Aspen Plus 13.1을 이용해서 전산모사를 수행하였다. 비교결과 순차배열이 비 순차배열에 비하여 초기 장치투자비용이나 운전비용에서 좀 더 우수한 것으로 나타났다.

• BMB Reports
• /
• 제40권4호
• /
• pp.562-570
• /
• 2007

Soluble or cell-bound IL-1 receptor accessory protein (IL-1RAcP) does not bind IL-1 but rather forms a complex with IL-1 and IL-1 receptor type I (IL-1RI) resulting in signal transduction. Synthetic peptides to various regions in the Ig-like domains of IL-1RAcP were used to produce antibodies and these antibodies were affinity-purified using the respective antigens. An anti-peptide-4 antibody which targets domain III inhibited 70% of IL-$1\beta$-induced productions of IL-6 and PGE2 from 3T3-L1 cells. Anti-peptide-2 or 3 also inhibited IL-1-induced IL-6 production by 30%. However, antipeptide-1 which is directed against domain I had no effect. The antibody was more effective against IL-$1\beta$ compared to IL-$1\alpha$. IL-1-induced IL-6 production was augmented by coincubation with PGE2. The COX inhibitor ibuprofen blocked IL-1-induced IL-6 and PGE2 production. These results confirm that IL-1RAcP is essential for IL-1 signaling and that increased production of IL-6 by IL-1 needs the co-induction of PGE2. However, the effect of PGE2 is independent of expressions of IL-1RI and IL-1RAcP. Our data suggest that domain III of IL-1RAcP may be involved in the formation or stabilization of the IL-1RI/IL-1 complex by binding to epitopes on domain III of the IL-1RI created following IL-1 binding to the IL-1RI.

• 한국수학교육학회지시리즈E:수학교육논문집
• /
• 제31권3호
• /
• pp.257-277
• /
• 2017

본 연구의 주된 목적은 크게 두 가지로 나눌 수 있다. 첫째, 최근 수학적 모델링이 강조되는 상황에서 보로노이 다이어그램과 들로네 삼각분할을 주제로 영재교육을 위한 수학적 모델링 프로그램을 개발하는 것이다. 둘째, 본 연구에서 개발한 수학적 모델링 프로그램을 실제 영재교육 수업에 적용한 결과를 분석하여 수학적 모델링 수업을 설계하는 현직교사와 융합형 영재프로그램을 개발하는 영재교사에게 도움을 주고자 한다.