• Microbiology and Biotechnology Letters
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• v.2 no.1
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• pp.45-50
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• 1974

1 With cysteinedesulfhydrase (E. C.4.4.1.1.) from Aerobactor aerogenes, an enzyme which catalyzes the stoichiometric conversion of L-cysteine to pyruvate, ammonia and sulfide, reversibility of the degradation of L-cysteine was investigated. It was found that the enzyme also catalized the reverse reaction of $\alpha$, $\beta$-elimination to synthesize L-cysteine derivatives from pyruvate, ammonia and sulfides when large amounts of substrates were added to the reaction mixtures. 2. The synthetic reaction by cysteinedesulfhydrase proceeded linearly with incubation time and enzyme concentrations. The optimal pH for the synthetic reaction was 10.0. 3. The results of the isolation and identification of the products showed that the L-cysteine derivatives synthesized by this enzymatic method were identical with S-methyl-L-cysteine and S-ethyl-L-cysteine respectively.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.3A
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• pp.196-204
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• 2002

The Sinusoidal Transform Coding(STC) is a vocoding scheme based on a sinusoidal model of a speech signal. The low bit-rate speech coding based on sinusoidal model is a method that models and synthesizes speech with fundamental frequency and its harmonic elements, spectral envelope and phase in the frequency region. In this paper, we propose the 2.4kbps low-rate speech coding algorithm using the sinusoidal model of a speech signal. In the proposed coder, the pitch frequency is estimated by choosing the frequency that makes least mean squared error between synthetic speech with all spectrum peaks and speech synthesized with chosen frequency and its harmonics. The spectral envelope is estimated using SEEVOC(Spectral Envelope Estimation VOCoder) algorithm and the discrete all-pole model. The phase information is obtained using the time of pitch pulse occurrence, i.e., the onset time, as well as the phase of the vocal tract system. Experimental results show that the synthetic speech preserves both the formant and phase information of the original speech very well. The performance of the coder has been evaluated in terms of the MOS test based on informal listening tests, and it achieved over the MOS score of 3.1.

• Journal of Conservation Science
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• v.35 no.3
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• pp.245-251
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• 2019

In this paper, conservation treatment processes for the wooden furniture in the Presidential Archives are introduced. Conservation treatment strategies for modern wooden cultural heritage have been increasingly studied. The current study uses materials similar to those used by the existing conservation treatment method. Material analysis showed that the chair(Lee136-2) and desk(Yun37) in the Presidential Archives are made of Dipterocarpaceae, and both are coated with two layers of varnishing. FT-IR analysis showed that the varnish has a similar spectrum to that of a nitrocellulose-based lacquer(Lee136-2) and top coat(Yun37) and confirmed that a synthetic material was used. Pollutants had adhered to the surface of the wooden furniture and it was also in structurally unstable condition because of cracks and damage to the varnish and wood. Therefore, a conservation treatment was carried out to restore the damaged areas to their original appearance using similar kinds of materials, based on data obtained by materials analysis.

• Earthquakes and Structures
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• v.5 no.2
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• pp.129-142
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• 2013

Linear and nonlinear time history analyses have been becoming more common in seismic analysis and design of structures with advances in computer technology and earthquake engineering. One of the most important issues for such analyses is the selection of appropriate acceleration time histories and matching these histories to a code design acceleration spectrum. In literature, there are three sources of acceleration time histories: artificial records, synthetic records obtained from seismological models and accelerograms recorded in real earthquakes. Because of the increase of the number of strong ground motion database, using and scaling real earthquake records for seismic analysis has been becoming one of the most popular research issues in earthquake engineering. In general, two methods are used for scaling actual earthquake records: scaling in time domain and frequency domain. The objective of this study is twofold: the first is to discuss and summarize basic methodologies and criteria for selecting and scaling ground motion time histories. The second is to analyze scaling results of time domain method according to ASCE 7-05 and Eurocode 8 (1998-1:2004) criteria. Differences between time domain method and frequency domain method are mentioned briefly. The time domain scaling procedure is utilized to scale the available real records obtained from near fault motions and far fault motions to match the proposed elastic design acceleration spectrum given in the Eurocode 8. Why the time domain method is preferred in this study is stated. The best fitted ground motion time histories are selected and these histories are analyzed according to Eurocode 8 (1998-1:2004) and ASCE 7-05 criteria. Also, characteristics of both near fault ground motions and far fault ground motions are presented by the help of figures. Hence, we can compare the effects of near fault ground motions on structures with far fault ground motions' effects.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.2
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• pp.185-193
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• 1997

The characteristics of $Na^+$-dependent cycloleucine uptake was investigated in OK cells with regard to substrate specificity and regulation by protein kinase C (PKC). Inhibition studies with different synthetic and natural amino acids showed a broad spectrum affinity to neutral amino acids regardless of their different side chains including branched or aromatic, indicating that the $Na^+$-dependent cycloleucine uptake in OK cells is mediated by System $B^o$ or System $B^o$-like transporter rather than the classical System A or ASC. Phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate, but not $4{\alpha}-PMA$ elicited a time-dependent biphasic stimulation of $Na^+$-dependent cycloleucine uptake, which produced early transient peak at 30 min and late sustained peak at 180min. Both the early and late stimulations by PMA were due to an increase in Vmax and not due to a change in Km. PKC inhibitors blocked both the early and late stimulation by PMA, while protein synthesis inhibitors blocked the late stimulation only. These results suggest the existence and regulation by PKC of System $B^o$ or System $B^o$-like broad spectrum transport system for neutral amino acids in OK cells.

• KSBB Journal
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• v.14 no.6
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• pp.682-687
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• 1999

Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. In order to investigate the function of VB-C as inducer in other strains, Streptomyces erythraeus was used as a test strain(parent). VB-C binding receptor gene was introduced into S. erythraeus(transformant) and the production of VBs and specific VB-C binding protein were analysed in parent and transformant. When 300ng/ml of the synthetic VB-C was added at 0, 20, 44 h cultivation of the parent and at 44 h cultivation of the transformant, the initial production times a antibiotics were shortened by more than 8 and 6 h, respectively. The transformant showed strong antibiotic activity against B. subtilis. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in S. erythraeus.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.151-155
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• 2004

In our previous studies for the development of new anticonvulsant of broad spectrum, we found that N-cbz-$\alpha$-aminoglutarimides showed significant anticonvulsant activities of broad spectrum enough to be recommended for the new anticonvulsants and their anticonvulsant activities were dependent on their imide substituent groups. Based on these results, various N-cbz--$\alpha$-amino-N-alkoxyglutarimides, where the imide N-H was substituted with the hydroxy and alkoxy group, were prepared and evaluated for their anticonvulsant activities using the Maximal electroshock seizure (MES) and Pentylenetetrazole induced seizure (PTZ) tests and also the rotorod test. A series of (R) or (S)-N-cbz--$\alpha$-amino-N-alkoxyglutarimides could be prepared from the corresponding (R) or (S)-N-cbz-glutamic acid following the usual synthetic procedure. Among them, (R)-N-cbz--$\alpha$-amino-N-hydroxyglutarimide ($ED_{50}$=86.25 mg/kg) was most active in the MES test. In the case of the PTZ test, (R)-N-cbz--$\alpha$-amino-N-benzyloxyglutarimide ($ED_{50}$= 62.5 mg/kg) was most active. Among the tested compounds, 2a-c, 3a, and 3b showed anticonvulsant activities in the MES and PTZ test. All of the tested compounds, except 2f and 3f, showed significant anticonvulsant activities in the MES or PTZ test. In addition, the neurotoxicities of these compounds were comparable to other anticonvulsant drugs.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.19 no.3
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• pp.152-158
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• 2009

Various fabrication methods have been used to synthesize sapphire which has qualities of jewelry well beyond the industrial class. Among them, the flux sapphire of Chatham Company which has as high value as jewelry was selected in order to compare natural and synthetic sapphire. First, the WD-XRF (Wavelength dispersive x-ray fluorescence spectrometer) was used to analyze the chemical composition of natural and synthetic sapphire. Although natural sapphire had very diverse chemical compositions, flux sapphire had small quantities of Mo, Pt and Pb elements in addition to the similar chemical ingredients to natural one. Pt is decisive proof of flux sapphire. Next, by investigating spectroscopic characteristics using UV-VIS Spectrophotometer after heat treatment at high temperatures of $1300^{\circ}C$ and $1500^{\circ}C$, the variation of 690 nm absorbance related to $Cr^{3+}$ was detected in the natural sapphire while those of the 690 nm absorbance (related to $Cr^{3+}$) as well as absorbance of 376 nm and 388 nm ($Fe^{3+}$) were seen in the flux sapphire. It was found that the difference in the absorbance variation of flux sapphire is greater than that of natural sapphire after heat treatment. The chemical composition and spectrum analysis were utilized to compare the natural sapphire and the flux synthetic sapphire.

• Applied Biological Chemistry
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• v.42 no.4
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• pp.356-360
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• 1999

Growth-inhibitory effects of citrus oils and agricultural fungicides, which were on the market, on several molds isolated from putrefied citrus fruits were investigated. When fungicidal activities of 11 kinds of synthetic agrochemicals against 6 species of molds, Alternaria alternata, Rhizopus sp., Botrytis cinerea, Monilia candida, Penicillium italicum and Penicillium digitatum, were investigated, agrochemicals containing mancozeb or fluazinam as an effective component had the broadest fungicidal spectrum. Agrochemicals containing iprodione, benomyl, azoxystrobin or thiophanate were less effective on the molds and those containing conazole derivative were intermediately effective. Resistance of the molds to the agrochemicals were species- and agrochemical-dependent. Among those molds tested, Penicillium italicum and Alternaria alternata showed relatively higher level of survival in the presence of synthetic fungicides. On the other hand, when the molds were exposed to citrus oils by direct contact, no cell could survive regardless of the species. The fungicidal activity of citrus oils was also confirmed by paper disk method and microscopic observation. These results suggested that citrus oils had broad killing activity against molds. Therefore, it would be necessary to design method for the application of citrus oils in order to improve post-harvest storage of citrus fruits.

• Korean Journal of Remote Sensing
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• v.34 no.6_3
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• pp.1383-1398
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• 2018

Sea surface wind is one of the most fundamental variables for understanding diverse marine phenomena. Although scatterometers have produced global wind field data since the early 1990's, the data has been used limitedly in oceanic applications due to it slow spatial resolution, especially at coastal regions. Synthetic Aperture Radar (SAR) is capable to produce high resolution wind field data. KOMPSAT-5 is the first Korean satellite equipped with X-band SAR instrument and is able to retrieve the sea surface wind. This study presents the validation results of sea surface wind derived from the KOMPSAT-5 backscattering coefficient data for the first time. We collected 18 KOMPSAT-5 ES mode data to produce a matchup database collocated with buoy stations. In order to calculate the accurate wind speed, we preprocessed the SAR data, including land masking, speckle noise reduction, and ship detection, and converted the in-situ wind to 10-m neutral wind as reference wind data using Liu-Katsaros-Businger (LKB) model. The sea surface winds based on XMOD2 show root-mean-square errors of about $2.41-2.74m\;s^{-1}$ depending on backscattering coefficient conversion equations. In-depth analyses on the wind speed errors derived from KOMPSAT-5 backscattering coefficient data reveal the existence of diverse potential error factors such as image quality related to range ambiguity, discrete and discontinuous distribution of incidence angle, change in marine atmospheric environment, impacts on atmospheric gravity waves, ocean wave spectrum, and internal wave.