• 미생물학회지
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• 제20권3호
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• pp.119-124
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• 1982

As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4\;1.0g, \;sucrose\;30.0g, \;MgSo_4{\cdot}7H_2O\;0.5g, \;FeCl_3{\cdot}6H_2O\;1.0mg\;thiamine\;200{\gamma}g$, and distilled water 1000ml, pH6.5.

• 미생물학회지
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• 제13권1호
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• pp.37-44
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• 1975

As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4$ 1.0g, sucrose 30.0g, $MgSo_4$ $7H_2$O 0.5g, $FeCl_36H_2$O 1.0mg thiamine 200.gamma.g, and distilled water 1000ml, pH6.5.

• 미생물학회지
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• 제38권4호
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• pp.250-250
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• 2002

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H/sup -/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of $H^-$-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• 한국환경보건학회지
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• 제30권2호
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• pp.161-166
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• 2004

The impact of dissolved wastewater constituents on the treatment of synthetic bisphenol A (BPA) solutions was investigated under a variety of reaction conditions. The laccase enzyme from Trametes vesicolor was used for the BPA treatment. The constituents studied included various inorganic salts, organic compounds and heavy metal ions. BPA degradation was inhibited by sulfate, thiosulfate, sulfide, nitrite, and cyanide ions at 25 mg/$\ell$, 100mg/$\ell$, 25 mg/$\ell$ 150 mg/$\ell$, and 2.5 mg/$\ell$, respectively. However, the inhibitive effects of sulfide and sulfite on BPA degradation were diminished by additional aeration to oxidize them. Formaldehyde significantly reduced the rate of BPA degradation at 1.0% among organic compounds studied. Among heavy metal ions tested, Fe(II) substantially suppressed BPA removal at 1 mM. MgCl$_2$ and CaCl$_2$ exhibited great inhibition of BPA degradation at 25mM.

• Journal of Microbiology
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• 제38권4호
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• pp.250-254
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• 2000

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H$\^$-/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of H$\^$-/-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.199-204
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• 2006

The purpose of this work was to evaluate the development of the anammox process by the use of granular sludge selected from a digestion reactor as a potential seed source in a lab-scale UASB (upflow anaerobic sludge blanket) reactor system. The reactor was operated for approximately 11 months and was fed by synthetic wastewater. After 200 days of feeding with $NH_4^+\;and\;NO_2^-$ as the main substrates, the biomass showed steady signs of ammonium consumption, resulting in over 60% of ammonium nitrogen removal. This report aims to present the results and to more closely examine what occurs after the onset of anammox activity, while the previous work described the start-up experiment and the presence of anammox bacteria in the enriched community using the fluorescence in situ hybridization (FISH) technique. By the last month of operation, the consumed $NO_2^--N/NH_4^+-N$ ratio in the UASB reactor was close to 1.32, the stoichiometric ratio of the anammox reaction. The obtained results from the influent-shutdown test suggested that nitrite concentration would be one key parameter that promotes the anammox reaction during the start-up enrichment of anammox bacteria from granular sludge. During the study period, the sludge color gradually changed from black to red-brownish.

• Biomolecules & Therapeutics
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• 제22권5호
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• pp.390-399
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• 2014

Chalcones (1,3-diaryl-2-propen-1-ones), a flavonoid subfamily, are widely known for their anti-inflammatory properties. Propenone moiety in chalcones is known to play an important role in generating biological responses by chalcones. In the present study, we synthesized chalcone derivatives structurally modified in propenone moiety and examined inhibitory effect on nitric oxide (NO) production and its potential mechanisms. Among the chalcone derivatives used for this study, TI-I-174 (3-(2-Hydroxyphenyl)-1-(thiophen-3-yl)prop-2-en-1-one) most potently inhibited lipopolysaccharide (LPS)-stimulated nitrite production in RAW 264.7 macrophages. TI-I-174 treatment also markedly inhibited inducible nitric oxide synthase (iNOS) expression. However, TI-I-174 did not significantly affect production of IL-6, cyclooxygenase-2 (COX-2) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), implying that TI-I-174 inhibits production of inflammatory mediators in a selective manner. Treatment of macrophages with TI-I-174 significantly inhibited transcriptional activity of activator protein-1 (AP-1) as determined by luciferase reporter gene assay, whereas nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activity was not affected by TI-I-1744. In addition, TI-I-174 significantly inhibited activation of c-Jun-N-Terminal kinase (JNK) without affecting ERK1/2 and p38MAPK, indicating that down-regulation of iNOS gene expression by TI-I-174 is mainly attributed by blockade of JNK/AP-1 activation. We also demonstrated that TI-I-174 treatment led to an increase in heme oxygenase-1 (HO-1) expression both at mRNA and protein level. Transfection of siRNA targeting HO-1 reversed TI-I-174-mediated inhibition of nitrite production. Taken together, these results indicate that TI-I-174 suppresses NO production in LPS-stimulated RAW 264.7 macrophages via induction of HO-1 and blockade of AP-1 activation.

• 분석과학
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• 제11권3호
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• pp.213-221
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• 1998

Bromide, chloride, fluoride, nitrite, nitrate, sulfite, sulfate, perchlorate, chlorate 및 chlorite가 포함된 시료를 capillary zone electrophoresis(CZE)와 indirect UV detection 방법으로 분리하였다. 음이온류의 분리는 running buffer로 5 mM ammonium dichromate, 10 mM ammonium acetate, 20 mM diethylenetriamine, 10% methanol(pH 9.3)을 사용하였으며 전압은 cathode에 15 kV를 공급하였다. 또한 최초로 CZE에서 음이온의 정량이 가능하도록 각 이온들에 대한 regression과 detection limit를 결정하였고, 이 분석방법은 톨루엔을 흡입한 사람의 뇨, 부검 혈액, 폐수 및 폭약 등 시료에 적용하였으며, 음이온의 함량을 ion chromatograph(IC)와 비교 검토하였다.

• 한국식품과학회지
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• 제13권3호
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• pp.233-240
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• 1981

합성배지를 이용한 팽나무 버섯균(菌)의 균사생육(菌絲生育)과 자실체(字實體) 형성에 대한 영양적 특성을 실험한 결과를 요약하면 다음과 같다. 1. 당질(糖質)중에서는 만니톨이 균사생육(菌絲生育)과 자실체 형성이 빠르고 자실체(字實體) 수량이 제일 많았으며, 유기산(有機酸)중에서는 숙신산에서만 자실체(字實體)가 빈약하게 형성되었고 에틸알콜과 글리세롤에서는 비교적 자실체(字實體) 형성도 빠르고 그 수율(收率)도 높았다. 2. 질소원(窒素源)중에서는 펩톤이 균사생육(菌絲生育)과 자실체(字實體) 형성이 빠르고 자실체(字實體)의 수량도 많았으며, 아미노산중에서는 glycine이 자실체(字實體) 형성도 빠르고 그 수율(收率)도 높았다. 아질산태(亞窒酸態) 질소(窒素)와 lysine, methionine은 균사(菌絲)도 생육하지 않았다. 3. 펩톤과 만니톨의 농도가 낮을수속 자실체(字實體) 형성(形成)은 늦으나 수율(收率)은 적었으며 농도가 높을수록 자실체(字實體) 형성은 늦으나 그 수율(收率)은 증가되는 경향을 보였다.

• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.410-414
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• 2000

The effect of the COD/N ratio on denitrification characteristics was evaluated for the development of a denitrification process. Activated sludge, acclimated to an anoxic condition, was used as the denitrifier consortium (mixture of denitrifying organisms) for enhanced nitrogen removal in a recirculating aquarium system. Synthetic wastewater containing nitrate was used as the influent solution and glucose was used as the carbon source for denitrification. The COD/N ratio varied within a range of 1.5-7.2. The denitrification efficiency was higher than 97% even at a COD/N ratio of 1.5. Under a theoretical COD/N ratio of 3.0, nitrite was detected, however, the amount was less than 1% of the total influent nitrogen. The number of both nitrate-reducing bacteria and denitrifying bacteria reached $3.5{\times}10^5/ml$ with a COD/N ratio of 1.5 after 45 days of operation.