• Mycobiology
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• 제39권3호
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• pp.158-163
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• 2011

This study was conducted to confirm the possible use of female Yangwonjam as a host for synnemata production of Isaria tenuipes in eight local areas in Korea. Silkworm pupation rate, infection rate and synnemata characteristics of I. tenuipes were examined. Normal silkworms had a higher pupation rate than silkworms inoculated with I. tenuipes. The pupae survival percentage of normal silkworm in cocoons was 92.5~97.6%, whereas it ranged from 91.1~95.6% in silkworms sprayed with I. tenuipes. Female Yangwonjam showed the highest survival percentage at 97.6% among the silkworm varieties tested. I. tenuipes infection rate of larvae of 5th instar newly-exuviated silkworms was 89.2~90.7% in the spring rearing season and 98.2~99.3% in the autumn rearing season. Synnemata production of I. tenuipes was excellent in female Yangwonjam with an incidence rate of 98.0% followed by male Yangwonjam (94.1%) and Baegokjam (93.3%) in the spring rearing season. Synnemata living weight ranged from 1.44~0.94 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight (1.44 g) in the spring rearing season. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and were similar in shape and color to wild synnemata collected in Korea.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.125-133
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• 2003

Chemical properties and physiological activities of the freeze-dried synnemata of Beauveria bassiana were examined. A proximate analysis showed that the synnemata consisted mainly of carbohydrate (49.86%), protein 11.36%), and a moisture content of 30.64%. It contained a low amount of crude ash (4.76%) and fat (3.38%). The carbohydrate was composed mainly of mannose (52.3%), galactose (31.5%), glucose (13.2%), and rhamnose (3%). Trace amounts of arabinose, xylose, and fructose were present. Major amino acids In the synnemata were glutamic acid, glycine, aspartic acid, arginine, threonine, alanine, valine, leucine, lysine, and aspartic acid with the amounts of 30.42, 25.22, 17.17, 15.12, 12.65, 15.23, 12.47, 11.47, 14.24, and 17.17 mg/g, respectively. Among extracts from the synnemata, the hot-water extract showed 67% of anticomplementary activity compared to that of the positive control, followed by ethyl acetate extract (17%) and methanol extract (15%). The hot-water extract also had anticoagulant activity with 55 sec of coagulating time and this fraction exhibited the most potent Intestinal immune system modulating activity. The methanol extract showed the highest inhibitory activity (25%) on the 12-O-tetradecanoyl phorbol-13-acetate-induced superoxide ($O_2^-$) generation, followed by hot-water extract (18%) and ethyl acetate extract (10%). The data in the present study indicate that the extract of Beauveria bassiana synnemata contains some healthful chemical ingredients and it could provide beneficial physiological activities. These features of the synnemata should be of interest to the food industry as well as other industrial fields.

• 한국버섯학회지
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• 제2권2호
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• pp.60-68
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• 2004

눈꽃동충하초 분생자경속을 형성하는데 액체배양을 이용한 접종원의 배지는 PDB가 가장 우수하였으며, 분생자경속 형성에 유리한 배양온도는 $20^{\circ}C$로 나타났다. 조명은 500lx.에서 가장 양호한 성장을 하였으며 광조사 시간은 18hr이 가장 유리한 분생자경형성 조건을 나타내었다. 알맞은 배지 조건은 번데기와 현미를 50 : 50의 비율에 증류수를 현미에 1 : 1비율로 첨가한 혼합배지에서 높게 나타났다. 번데기배지는 원형상태와 분쇄상태 중에서는 분쇄된 상태에서 번데기배지의 공극률이 높아 균사의 침입이 용이하여 분생자경속 회수율과 균사의 배지내에 형성이 원형상태의 번데기보다 더욱 높게 나타났으며, 균사의 생장도 역시 분쇄한 번데기에서 더욱 빠르고 고르게 나타났다. 종균 접종량은 $15m{\ell}$의 종균을 접종하면 분생자경속이 잘 형성되었다. 눈꽃동충하초 재배에 가장 안정하게 재배하려면 분쇄한 번데기에 현미와 혼합한 배지에 종균 $15m{\ell}$를 접종하여 24 에서 발이를 시킨 후 $20^{\circ}C$의 배양실에서 재배하는 것이 가장 좋은 것으로 나타났다.

• 한국응용곤충학회지
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• 제49권1호
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• pp.43-47
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• 2010

백강균(Beauveria bassiana)의 분생자병속 형성 조건 구명을 위해 본 실험을 수행하였다. 균은 제주도 설악산 일대에서 치사한 참매미로부터 분리되었다. 분생자병속 형성 유도 배지는 현미와 누에번데기를 원료로 하여 90:10 비율로 조제한 경우가 가장 효과적이었으며, 기타 배지 상에서는 분생자병속 발생이 관찰되지 않았다. 분생자병속은 공기 유입량이 최소일 때 형성이 효과적이었으며, 분생자병속 발생의 최적 온도는 $20^{\circ}C$, 배지 수분함량은 60% 및 조도 300 lux이었다. 또한, 증식에 활용한 종균은 액체배양균이 무성포자보다 분생자병속 형성에 더욱 효과적이었다.

• Mycobiology
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• 제38권3호
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• pp.180-183
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• 2010

This study was conducted out to select a silkworm variety suitable for synnemata production of Isaria tenuipes. Four kinds of the mulberry silkworm varieties, Bombyx mori, were hybridized using a Japanese parental line and a Chinese parental line, and used to test for synemata formation in I. tenuipes. The larval period of normal silkworms was 22 hr longer than the silkworms inoculated with this fungus. Among the silkworm varieties tested, Hachojam had the shortest larval period with 23.02 days. The non-cocooning silkworm had a shorter larval period than the cocoon producing silkworms. The pupation rate of normal silkworms was about 9% higher than that of silkworms sprayed with I. tenuipes. Hachojam had the highest infection rate at 99.8%, but no significant difference was observed for the infection rate by silkworm variety. The production of synnemata was the best in JS171 $\times$ CS188 with an incidence rate of 99.3%, followed by Hachojam, and Chugangjam. The synnemata produced from Hachojam were the heaviest and showed white or milky-white in color.

• 한국식품영양과학회지
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• 제32권7호
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• pp.997-1001
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• 2003

밀가루와 백강균 자실체 분말을 각각 99:1, 97:3 및 95:5(w/w)로 혼합한 복합분을 이용하여 그 제빵적성을 비교하였다. 대조구의 비용적은 4.69 mL/g bread로 나타났으며, 자실체 분말을 1%와 3% 첨가한 자실체-빵의 비용적은 대조구의 비용적과 비슷한 용적을 나타났다. 그러나 자실체 분말을 5% 첨가한 자실체-빵의 비용적은 대조구에 비해 크게 감소하는 결과를 나타내었다. 자실체 분말을 1%와 3% 첨가한 자실체-빵의 L값은 대조구에 비하여 약간의 감소를 나타내었으나, 5%의 빵의 L값은 대조구에 비해 크게 감소하였다. 자실체 분말의 첨가농도가 높아질수록 자실체-빵의 a값과 b값은 각각 대조구에 비해 증가하는 결과를 나타내었다. 대조구의 경도는 11.27 N이었으며, 자실체 분말을 1%와 3% 첨가한 자실체-빵의 경도는 대조구와 비슷하였으나 5%를 첨가한 자실체-빵의 경도는 대조구에 비해 크게 증가하였다. 자실체 분말을 1%와 3%를 각각 첨가한 자실체-빵의 탄성, 껌성 및 씹힘성은 대조구와 차이를 나타내지 않았다. 전반적인 기호도에 대한 관능검사 결과 자실체 분말 1%와 3%를 첨가한 빵은 대조구와 비슷하였으나, 자실체 분말 5%를 첨가한 빵은 대조구에 비해 크게 감소하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권1호
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• pp.1-4
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• 2011

This study was conducted to test the use of colored cocoon silkworm, Golden Silk, as a host for the fruiting body production of Cordyceps mushroom. Golden Silk variety had a shorter larval period than Yangwonjam at $25.3^{\circ}C$ and 78.5% RH: The average larval period of the Golden Silk was 24 days and 7 hr, and that of the Yangwonjam was 24 days and 15 hr. In the infection test of I. tenuipes on silkworm, the larval period of normal silkworms was longer than the silkworms inoculated with I. tenuipes in both Golden Silk and Yangwonjam. The pupae survival percentage of Golden Silk was 94.4%, whereas it was 91.9% in Yangwonjam. Golden Silk had a higher pupation rate than Yangwonjam. The pupation rate of normal silkworms was about 5% higher than that of silkworms inoculated with I. tenuipes. Infection rate of I. tenuipes into larvae of 5th instar newly exuviated silkworm was 93.8% in Golden Silk, and 96.9% in Yangwonjam. Synnemata production of I. tenuipes was better in Yangwonjam with an incidence rate of 99.8% than Golden Silk with 98.6%. The average synnemata weight of Golden Silk was 1.12 g and that of Yangwonjam 1.29 g. Golden Silk had a lower synnemata weight than Yangwonjam. But no significant difference was observed in synnemata weight varieties. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and similar in shape and color to wild synnemata collected in Korea.

• 한국식품영양학회지
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• 제16권2호
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• pp.158-164
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• 2003

백강균 자실체를 첨가한 복합분의 제면적성에 미치는 영향을 조사하였으며 면의 색도, 조리특성, 기계적인 조직감 및 관능검사를 실시하였다. 자실체-건면과 조리면의 L값은 첨가농도에 관계없이 각각 대조구와 차이를 나타내지 않았으나 자실체-건면과 조리면의 a값과 b값은 대조구에 비해 각각 증가하였다. 자실체를 첨가한 조리면의 중량, 부피 및 수분흡수율은 자실체의 첨가농도에 관계없이 대조구와 큰 차이를 나타내지 않았으나 자실체를 첨가한 조리면의 국물의 탁도는 대조구에 비해 증가하였다. 자실체-건면의 파쇄력은 자실체의 첨가농도에 관계없이 모두 대조구와 같은 값을 나타내었다. 자실체의 첨가농도에 관계없이 자실체-조리면의 견고성과 껌성은 각각 대조구와 같은 값을 나타내었다. 또한 자실체 첨가농도가 1%일때 자실체-조리면의 탄성, 씹힘성, 응집성 및 부착성은 대조구와 같았으나, 자실체 첨가농도가 3% 및 5% 일때 자실체-조리면의 탄성, 씹힘성, 응집성 및 부착성이 대조구에 비해 감소하였다. 자실체 분말을 1%와 3%를 첨가하여 제조한 조리면의 색, 맛, 향, 조직감 및 전체적인 기호도는 대조구에 비해 차이를 나타내지 않았다.

• Mycobiology
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• 제35권4호
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• pp.230-234
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• 2007

In order to breed a Cordyceps bassiana isolate that stably forms fruiting body in artificial cultivation, isolates derived from subculturing and single spores were tested through mating. From C. bassiana EFCC 783, three subcultured isolates EFCC 2830, EFCC 2831 and EFCC 2832 were obtained and fourteen single conidial isolates were obtained from these three subcultured isolates. Two different morphological types were found in the fourteen single conidial isolates. One type was able to form synnemata and another type was not able to form synnemata. Since switch of morphological type was not observed despite their continuous subculturing, cross was performed between the two types and the formation of fruiting body was examined. Ascospores were obtained from a selected fruiting body formed by hybrid of the cross. Self-cross and combinational cross of the ascospore-derived isolates generated hybrids that stably produce high quality fruiting body in artificial media.

• Mycobiology
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• 제38권2호
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• pp.118-121
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• 2010

Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.