Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Synnemata Production Using Silkworm Variety, Female Yangwonjam by Isaria tenuipes

  • Ji, Sang-Duk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Sung, Gyoo-Byung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Pil-Don (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Kee-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Yong-Soo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Nam-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Woo, Soon-Ok (National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Sang-Mi (National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, In-Pyo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ha, Nam-Gyu (Environment-friendly Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Services)
  • Received : 2011.05.31
  • Accepted : 2011.07.20
  • Published : 2011.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to confirm the possible use of female Yangwonjam as a host for synnemata production of Isaria tenuipes in eight local areas in Korea. Silkworm pupation rate, infection rate and synnemata characteristics of I. tenuipes were examined. Normal silkworms had a higher pupation rate than silkworms inoculated with I. tenuipes. The pupae survival percentage of normal silkworm in cocoons was 92.5~97.6%, whereas it ranged from 91.1~95.6% in silkworms sprayed with I. tenuipes. Female Yangwonjam showed the highest survival percentage at 97.6% among the silkworm varieties tested. I. tenuipes infection rate of larvae of 5th instar newly-exuviated silkworms was 89.2~90.7% in the spring rearing season and 98.2~99.3% in the autumn rearing season. Synnemata production of I. tenuipes was excellent in female Yangwonjam with an incidence rate of 98.0% followed by male Yangwonjam (94.1%) and Baegokjam (93.3%) in the spring rearing season. Synnemata living weight ranged from 1.44~0.94 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight (1.44 g) in the spring rearing season. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and were similar in shape and color to wild synnemata collected in Korea.

Keywords

References

  1. Kang PD, Sohn BH, Lee SU, Hong SJ. Breeding of a new non-cocooning silkworm variety, Hachojam, suitable for autumn rearing season. Int J Indust Entomol 2002;4:77-81.
  2. Kang PD, Lee SU, Jung IY, Sohn BH, Kim YS, Kim KY, Kim MJ, Hong IP, Lee KG, Park KY. Breeding of new silkworm variety golden silk, a yellow cocoon color for spring rearing season. Korean J Seric Sci 2007;49:14-7.
  3. Kang PD, Jung IY, Kim KY, Kim MJ, Sohn BH, Lee GG. Breeding of two new silkworm varieties with peculiar larval mark, "Eolrukmal" and "Hukpyobeom". Int J Indust Entomol 2010;20:115-6.
  4. Kang PD, Kim KM, Sohn BH, Woo SO, Ryu KS. Breeding of "Yangwonjam" a both parent sex-limited larval marking variety suitable for spring and autumn rearing season. Korean J Seric Sci 2000;42:24-7.
  5. Sung JM, Lee HK, Choi YS, Kim YY, Kim SH, Sung GH. Distribution and taxonomy of entomopathogenic fungal species from Korea. Kor J Mycol 1997;25:239-52.
  6. Cory JG, Suhadolnik RJ, Resnick B, Rich MA. Incoporation of cordycepin (3'-deoxyadenosine) into ribonucleic acid and deoxyribonucleic acid of human tumor cells. Biochim Biophys Acta 1965;103:646-53.
  7. Furuya T, Hirotani M, Matsuzawa M. $N^6$-(2-hydroxyethyl) adenosine, a biologically active compound from cultured mycelia of Cordyceps and Isaria species. Phytochemistry 1983;22:2509-12.
  8. Liu JB. Forestry history of Ganzi Tibetan Autonomous Prefecture (Chin.). Chengdu: Sichuan Kexue Jisu Chubanshe; 1994. p. 1-323.
  9. Ha NG, Kim SY, Kang JH, Kang PD, Sung GB, Hong IP. Biological activities and cultural characteristics of an entomogenous fungus, Paecilomyces tenuipes (Peck) Samson. Korean J Seric Sci 2005;47:12-7.
  10. Kang PD, Sung GB, Kim KY, Kim MJ, Hong IP, Ha NG. Breeding of a silkworm variety for synnemata production of Isaria tenuipes. Mycobiology 2010;38:180-3.
  11. Kobayasi Y. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans Mycol Soc Jpn 1982;23:329-64.
  12. Spatafora JW, Blackwell M. Molecular systematics of unitunicate perithecial ascomycetes: the Clavicipitales- Hypocreales connection. Mycologia 1993;85:912-22.
  13. Sung JM. The insects-born fungus of Korea in color. Seoul: Kyohak Publishing Co. Ltd.; 1996. p. 62-72.