• Title/Summary/Keyword: Synchrotron X-ray 미세영상

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Flow Measurement in Xylem Vessels of a Bamboo Leaf Using Synchrotron X-ray Micro Imaging (Synchrotron X-ray 미세영상기법을 이용한 식물 목질부 내부 수액 유동의 계측)

  • Kim Yang Min;Lee Sang-Joon
    • 한국가시화정보학회:학술대회논문집
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    • 2003.11a
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    • pp.83-86
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    • 2003
  • Synchrotron X-ray micro imaging technique was employed to non-invasively monitor the water flow inside xylem vessels in a bamboo leaf. The phase contrast X-ray images clearly visualized plant anatomy and the rise of a water front inside the vessels. Consecutive X-ray images taken for 60 seconds revealed water rise kinetics against gravity in the xylem of a cut dry leaf taken from a bamboo tree. For the first time, traces of water rise, variation of contact angle between water and xylem wall as well as the internal structure of xylem were obtained. In xylem vessels, a repeating flow pattern has a typical flow velocity of $30.7{\mu}m/s$ and faster flow is established intermittently. It is concluded that the transmission type of X-ray micro imaging can be used as a powerful tool to investigate the ascent of sap in the xylem vessels at a resolution higher than that of MRI.

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In Vivo Visualization of Flow in Xylem Vessels of a Bamboo Leaf Using Synchrotron X-ray Micro Imaging Technique (Synchrotron X-ray 미세영상기법을 이용한 식물 목질부 내부 수액 유동의 계측)

  • Kim, Yang-Min;Lee, Sang-Joon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.11
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    • pp.1612-1617
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    • 2003
  • Synchrotron X-ray micro imaging technique was employed to non-invasively monitor the water flow inside xylem vessels in a bamboo leaf. The phase contrast X-ray images clearly visualized plant anatomy and the rise of a water front inside the vessels. Consecutive X-ray images taken for 60 seconds revealed water rise kinetics against gravity in the xylem of a cut dry leaf taken from a bamboo tree. For the first time, traces of water rise, variation of contact angle between water and xylem wall as well as the internal structure of xylem were obtained. In xylem vessels, a repeating flow pattern has a typical flow velocity of 30.7$\mu\textrm{m}$/s and faster flow is established intermittently. It is concluded that the transmission type of X-ray micro imaging can be used as a powerful tool to investigate the ascent of sap in the xylem vessels at a resolution higher than that of MRI.

Synchrotron Radiation Imaging of Breast Tissue Using a Phase-contrast Hard X-ray Microscope (경 엑스선 위상차 현미경을 이용한 유방 조직의 방사광 영상)

  • Jeong, Young-Ju;Bong, Jin-Gu;Park, Sung-Hwan
    • Progress in Medical Physics
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    • v.22 no.3
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    • pp.117-123
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    • 2011
  • Synchrotron radiation (SR) imaging enables us to observe internal structures of biologic samples without staining. In this study, we obtained X-ray microscopic images of human breast tissues with 11.1 KeV hard X-ray microscope of the Pohang light source and used zone plates and phase-contrast technique to get high resolution X-ray images. Hard X-ray microscopic images of fibrocystic change and breast cancer tissues with a spatial resolution of 60 nm were obtained and from these images, we could observe the micro-structures of human breast tissue. Also we analyzed and compared these images, which revealed distinct features of each condition. In conclusion, SR imaging with phase-contrast hard X-ray microscope for medical application, especially in breast disease can give some useful information for clinical research.

Synchrotron X-ray Micro-imaging Technique for Simultaneous Measurement of Size and Velocity of Micro-bubbles (X-ray 미세 영상기법을 이용한 미세기포의 크기 및 속도 동시측정)

  • Kim, Seok;Lee, Sang-Joon
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1744-1748
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    • 2004
  • It is important to measure precisely the size and velocity of micro-bubbles used in various field. The synchrotron X-ray micro-imaging technique was employed to measure the size and velocity of micro-bubbles moving in an opaque tube simultaneously. Phase contrast images were obtained at interfaces of micro-bubbles between water and air due to their different refractive indices. The X-ray micro-imaging technique was found to measure an optical fiber with an accuracy of 0.2%. Micro-bubbles of $10{\sim}60{\mu}m$ diameter moving upward in an opaque tube (${\phi}=2.7mm$) were tested to measure bubble size and up-rising velocity. For DI water, the measured velocity of micro-bubbles is nearly proportional to the square of bubble size, agreed well with the theoretical result. In addition, the synchrotron X-ray micro-imaging technique can measure accurately the size and velocity of several overlapped micro-bubbles.

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Micrometer Spatial Resolution Imaging System Using Synchrotron X-ray (Synchrotron X-선을 이용한 Micrometer 공간 분해능 영상시스템)

  • 홍진오;정해조;정하규;제정호;김은경;유형식;김희중
    • Journal of Biomedical Engineering Research
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    • v.22 no.2
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    • pp.165-169
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    • 2001
  • 최근 포항 방사광 가속기 연구소에 미세구조 X-선 영상 실험을 위한 5C1 방사광(Synchrtoron Radiation) 빔라인이 건설되었다. 광대역의 에너지 스펙트럼을 가진 방사광 X-선이 물체를 투과한 후 CdWO$_{4}$ scintillator에 의해 가시광선으로 바뀌고, 그 빛을 CCD 카메라로 받아들여 영상을 획득하게 된다. 방사광 X-선은 일반 의료진단용 X-선에 비하여 위상이 일치하고, 평행하며, 그 양이 풍부한 특성들을 갖고 있다. 방사광 영상시스템과 X-선 유방촬영 시스템에서 영상을 획득하여 영상특성들을 비교, 분석하였다. 고-분해능 X-선 시험 패턴(20 line pairs mm$^{-1}$), 유방촬영 팬텀, 파라핀에 고정한 인체 유방암조직과 포르말린에 고정한 인체 유방암조직, 그리고 capillary tube내 micro-bubbles등의 방사광 영상은 기존의 X-선 유방촬영시스템에서 얻은 영상보다 분해능이 뛰어나고 영상질도 우수하였다. 방사광 X-선 영상시스템은 micrometer 공간 분해능 영상을 획득할 수 있어 많은 기초분야의 영상연구와 의료영상분야에서도 활발하게 활용될 것으로 기대된다.

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X-ray Micro-Imaging Technique for Simultaneous Measurement of Size and Velocity of Micro-Bubbles (X-ray 미세 영상기법을 이용한 미세기포의 크기 및 속도 동시 측정기술 개발)

  • Kim, Seok;Lee, Sang-Joon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.28 no.6
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    • pp.659-664
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    • 2004
  • It is important to measure precisely the size and velocity of micro-bubbles used in various field. The synchrotron X-ray micro-imaging technique was employed to measure the size and velocity of micro-bubbles moving in an opaque tube simultaneously. Phase contrast images were obtained at interfaces of micro-bubbles between water and air due to their different refractive indices. The X-ray micro-imaging technique was found to measure an optical fiber with an accuracy of 0.2%. Micro-bubbles of 20∼60$\mu\textrm{m}$ diameter moving upward in an opaque tube (${\Phi}$=2.7mm) were tested to measure bubble size and up-rising velocity. For DI water, the measured velocity of micro-bubbles is nearly proportional to the square of bubble size, agreed well with the theoretical result. In addition, the synchrotron X-ray micro-imaging technique can measure accurately the size and velocity of several overlapped micro-bubbles.

In Vivo Visualization of Flow in Xylem Vessels of a Bamboo Using X-ray Micro-imaging Technique (X-ray 미세영상기법을 이용한 식물 목질부 내부 수액 유동의 계측)

  • Kim, Yang-Min;Lee, Sang-Joon
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1693-1696
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    • 2004
  • Synchrotron X-ray micro-imaging technique was employed to monitor non-invasively the refilling process of water inside the xylem vessels in bamboo leaves. The consecutive phase-contrast X-ray images clearly show both plant anatomy and the transport of water inside the xylem vessels. Traces of water-rise, vapor bubbles and variations of contact angle between the water front and the xylem wall were measured in real time. During the refilling process, air bubbles are removed when the rising water front halts at a vessel end for a while. Subsequently, it starts rising again at a higher velocity than the normal refilling speed. Repeated cavitation seems to deteriorate the refilling ability in xylem vessels. In dark environment, the water refilling process in xylem vessels is facilitated more effectively than in bright illuminated conditions. Finally, X-ray micro-imaging was famed to be a powerful, high resolution, real time imaging tool to investigate the water refilling process in xylem vessels.

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Synchrotron Radiation Imaging of Tissues Using Phase Contrast Technique (방사광 위상차 현미경을 이용한 생체조직의 미세구조 영상)

  • Kang, Bo-Sun;Lee, Dong-Yeol;Kim, Ki-Hong
    • Journal of the Korean Society of Radiology
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    • v.2 no.2
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    • pp.23-30
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    • 2008
  • X-ray microscopy with synchrotron radiation(SR) might be a useful tool for novel x-ray imaging in the clinical and laboratory settings. Microscopically, it enables us to observe detailed structure of animal organs samples with a great magnification power and an excellent resolution. The phase contrast mechanisms in image by X-ray are described. The phase-contrast X-ray imaging with SR from in-vivo and in-vitro mouse tail, rat nerve and rat lung were obtained with an 8 KeV monochromatic beam. The visual image was magnified using 10x microscope objective lens and captured using an digital CCD camera. The results showed more structural details and high resolution images with SR imaging system than conventional X-ray radiography system. The SR imaging system may have a potential for imaging in biological researches, material applications and clinical radiography.

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A Study of the 3D Anatomical Structure Analysis of Leech Using Hard X-ray Micro Tomography by Synchrotron Radiation (방사광 경 엑스선 마이크로 단층촬영을 이용한 거머리의 해부학적 3차원 구조분석)

  • Lee, Ji Won;Lee, On Seok
    • The Journal of the Korea Contents Association
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    • v.16 no.12
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    • pp.70-77
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    • 2016
  • In this study, we aim to obtain the fundamental data needed for the objective analysis and bio-mimetics of leeches, using x-ray imaging with radiation that allows 3D analysis of the detailed anatomic structures of leeches. It was confirmed that through using radiation, detailed images of leeches can be obtained, allowing 3D analysis of leeches' anatomical structures. Also, since the data obtained through the radiation allows you to observe the micro-structure, it can serve as a good resource for component analysis, as well as physiological and functional research. More, it is hoped to contribute to further research in the areas of bio-mimetics using leeches.

Verification of the Experimental Correlation for Dynamic Contact Angle by Visualizing Interfaces of Water-Glycerol Mixture Slug in a Hydrophobic Microtube via Synchrotron X-ray Imaging (방사광 X-선 영상법을 이용한 소수성 마이크로 관 내 물-글리세롤 혼합물 슬러그 계면 가시화를 통한 동적접촉각 상관식 검증)

  • Jang, Jin Gyu;Kim, Young Hyun;Kim, Kyoung Joon;Lee, Junghoon;Lee, Yeon Won;Yu, Dong In
    • Korean Chemical Engineering Research
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    • v.60 no.1
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    • pp.145-150
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    • 2022
  • Dynamic contact angles have investigated by numerous researchers for understanding interfacial behavior at moving contact lines However, due to limitation of visualization techniques, previous experiments for dynamic contact angles have conducted limitedly in hydrophilic capillary tubes based on visible ray. Recently, there is continuous need for research on dynamic contact angles in hydrophobic capillary tubes on various research and industrial fields. Therefore, in this study, we measure the dynamic contact angles of water-glycerol mixture slug in hydrophobic microtubes using synchrotron X-ray imaging. Based on the visualized data, we verified the previous experimental correlations for dynamic contact angles.