• International Journal of Industrial Entomology and Biomaterials
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• 제12권1호
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• pp.21-28
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• 2006

Peroxidase activity was measured in brown leaf spot pathogen (Myrothecium roridum) inoculated potted mulberry (Morus alba) during pre-symptomatic to various symptom development stages and compared with corresponding healthy leaf tissues. The enzyme showed a pH optimum of 7.0 and the activity was linearly increased up to 15 min of incubation. The peroxidase had a broad substrate specificity and the rates of oxidation were in the rank of pyrogallol> guaiacol> ascorbate at pH 7.0. Catechol at 10 mM inhibited 89% of guaiacol-peroxidase and 76% pyrogallol-peroxidase activities, indicated higher non-specific peroxidation in pyrogallol dependent assay system in mulberry than guaiacol. The optimum requirement for the guaiacol dependent assay was 0.2 ml (${\approx}40-60{\mu}g$ equivalent of protein) of crude enzyme source. Excepting the 8th leaf from the apex, the peroxidase activity did not vary appreciably in different leaf positions. In pre-symptomatic phases, an initial (1 to 5 min) rise of peroxidase activity was noticed in inoculated leaves, and then maintained a plateau up to 300 min. In contrary, non-infected tissue showed a slightly increased trend of enzyme level up to 420 min. In infected tissue, a sharp transient increase (3.1 fold) of peroxidase activity appeared between 300 - 420 min post infections. Afterwards, significantly different but steady maintenance of enzyme levels were observed in two treatments. On the other hand, during symptom development, a sharp increase in peroxidase activity was noticed up to 4th grade of lesion appearance (25.1 % to 50% of leaf area infection), and then declined slightly. However, in non-infected but same age healthy leaves, such huge fluctuations of enzyme level did not apparent. A high positive correlation $(R^2=0.92)$ between peroxidase activity and leaf spot development grades was also marked. The result implies that pre-symptomatic burst (between 1 - 5 and 300 - 420 min) and subsequent increased trend of guaiacol peroxidase activity may require for the symptomatic manifestation of Myrothecium leaf spot in mulberry.

• 대한예방한의학회지
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• 제17권1호
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• pp.13-25
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• 2013

Objectives : This study was conducted to analyze the stress, coping and physical symptoms relevant to factors influencing physical symptoms on high school girls. Methods : The subjects were 521 high school girls in I city. The data was collected by using structured questionnaire. T-test, ANOVA, Pearson's correlation and Stepwise-multiple regression were performed by SPSS/PC 17.0. Results : Study-related stress were higher in the freshmen than sophomore (p=.001). The sophomore have highest level in family (F=5.32, p=.005), friends (F=3.13, p=.044), and appearance-related stress (F=5.49, p=.004). Poor grades in their studies were related to family-related stress (p=.010) and study-related stress (p=.009), They complained severe discomfort, displeasure, and G-I symptom in order. The more physical symptoms were, the more coping with their stress do (p=.001). Factors affecting physical symptoms are study-related stress (${\beta}$=.38), unhealthy (${\beta}$=.16), friends (${\beta}$=.14), active coping with stress (${\beta}$=.15) and sophomore (${\beta}$=.11), family (${\beta}$=.11), and they explained 33.3% of variables. Conclusions : Students have to learn how to cope with their stress. Therefore, it is needed program development to improve stress management for high school girls.

• 임상간호연구
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• 제16권3호
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• pp.175-186
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• 2010

Purpose: Delirium can be a highly prevalent symptom in intensive care units but it may still be under-recognized despite its relation with inclined morbidity, mortality, cost, and readmission. Therefore, this study aimed to develop a protocol that covers risk factors and non-pharmacological interventions to prevent delirium in ICU patients. Methods: This study was conducted using methodological design, and it followed the Scottish Intercollegiate Guideline Network (SIGN) guideline development steps: 1) the scope of protocol was decided (population, intervention, comparison, and outcomes); 2) guidelines, systematic reviews, and protocols were reviewed and checked using methodology checklist; 3) the level of evidence and recommendation grades was assigned; 4) the appropriateness of recommendations was scored by experts; 5) the final protocol & algorithm was modified and complemented. Results: The evidence-based delirium prevention protocol was completed that includes predisposing factors, precipitating factors and recommendations with evidenced grades. Conclusion: This protocol can be used as a guide nurses in screening patients with high risk factors of delirium as well as in intervening the patients non-pharmacologically to prevent delirium.

• 한국학교ㆍ지역보건교육학회지
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• 제20권2호
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• pp.93-107
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• 2019

Objectives: The purpose of this study is to provide basic data on the developement of oral health programs to maintain correct oral health in adolescence by identifying the relevance of health-related behavior and symptom experience to adolescents. Methods: Using the 14th(2018) online survey of youth health behavior, this study was conducted on students in grades1 middle school to grade5 high school in Korea and 30,463 boys, 29,577 girls were selected as the final analysis targets. Results: During the oral disease symptom experience, there was a statistically significant difference in smoking experience with teeth pain controlled general characteristics during eating. Conclusions: It is expected that it will be used as basic dat for the development of oral health programs to maintain the correct oral health status of adolescents by identifying the effects of health-related behaviors and related variables on oral diseases in Korea.