• Korean Chemical Engineering Research
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• v.58 no.1
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• pp.127-134
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• 2020

The adsorption characteristics of the major tar compound, acenaphthene, derived from Taxus chinensis by the commercial adsorbent Sylopute were investigated using different parameters such as initial acenaphthene concentration, adsorption temperature, and contact time. Out of Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models, adsorption data were best described by Langmuir isotherm. The adsorption kinetics was evaluated by pseudo-first-order, pseudo-second-order and intraparticle diffusion models. The pseudo-second-order model was found to explain the adsorption kinetics most effectively. Thermodynamic parameters revealed the feasibility, nonspontaneity and exothermic nature of adsorption. In addition, the isosteric heat of adsorption was independent of surface loading indicating the Sylopute used as an energetically homogeneous surface.

• Korean Chemical Engineering Research
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• v.58 no.1
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• pp.113-121
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• 2020

In batch experiments, the adsorption of 7-epi-10-deacetylpaclitaxel was studied using Sylopute. Experimental equilibrium data were applied to Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models. Among the four isotherm models tested, the Langmuir isotherm model gave the highest accuracy. The adsorption capacity was found to decrease with increases in temperature and the adsorption of 7-epi-10-deacetylpaclitaxel onto Sylopute was a favorable physical process. Adsorption kinetic data agreed very well with the pseudo-second-order kinetic model, while boundary layer diffusion and intraparticle diffusion did not play a key role in the adsorption process. The process of 7-epi-10-deacetylpaclitaxel adsorption onto Sylopute was exothermic and nonspontaneous. Also, the adsorption isosteric heat was independent of surface loading indicating an energetically homogeneous adsorbent.

• Korean Chemical Engineering Research
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• v.57 no.2
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• pp.219-224
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• 2019

In this study, the adsorption characteristics of cephalomannine on commercial adsorbent Sylopute were investigated using different parameters such as adsorption temperature, time, and initial cephalomannine concentration for the efficient separation of Taxus chinensis-derived cephalomannine by adsorption process. The Temkin isotherm model showed good fit to the equilibrium adsorption data. The adsorption capacity decreased with increasing temperature and the adsorption of cephalomannine onto Sylopute was physical in nature. Adsorption kinetic data fitted well with pseudo-second-order kinetic mode. According to the intraparticle diffusion model, film diffusion and intraparticle diffusion did not play a key role in the entire adsorption process. The process of cephalomannine adsorption onto Sylopute was exothermic and spontaneous. In addition, the isosteric heat of adsorption was constant even with variation in surface loading indicating homogeneous surface coverage.

• Korean Chemical Engineering Research
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• v.57 no.2
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• pp.210-218
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• 2019

Batch experiment studies were carried out on the adsorption of the major tar compound, 2-picoline, derived from the plant cell cultures of Taxus chinensis, using Sylopute while varying parameters such as initial 2-picoline concentration, contact time and adsorption temperature. The experimental data were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherm models. Comparison of results revealed that the Langmuir isotherm model could account for the adsorption isotherm data with the highest accuracy among the four isotherm models considered. From the analysis of adsorption isotherms, it was found that adsorption capacity decreased with increasing temperature and the adsorption of 2-picoline onto Sylopute was favorable. The kinetic data were well described by the pseudo-second-order kinetic model, while intraparticle diffusion and boundary layer diffusion did not play a dominated role in 2-picoline adsorption according to the intraparticle diffusion model. Thermodynamic parameters revealed the exothermic, irreversible and non-spontaneous nature of adsorption. The isosteric heat of adsorption decreased as surface loading ($q_e$) increased, indicating a heterogeneous surface.

• Korean Chemical Engineering Research
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• v.52 no.4
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• pp.497-502
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• 2014

Biomass-derived tar and waxy compounds have a highly negative effect on the separation and purification of paclitaxel and should be removed prior to final purification. Adsorbent treatment is a simple, efficient method for removal of tar and waxy compounds from plant cell cultures. In this study, we optimized the important process parameters (adsorption temperature, time, solvent type and adsorbent amount) of adsorbent treatment with Sylopute to remove the tar and waxy compounds in a pre-purification step. The optimal adsorption temperature, adsorption time, solvent type, and crude extract/Sylopute ratio were $30^{\circ}C$, 15 min, methylene chloride, and 1:1(w/w), respectively. This result could be confirmed by HPLC analysis of the absorbent after treatment and TGA of the organic substances that were bound to the adsorbent. In adsorbent treatment step, the purity seemed to show a small improvement but this treatment had a significant effect on convenience and feasibility of following steps by the removal of tar and waxy compounds.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.89-93
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• 2016

In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and $20^{\circ}C$, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.