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http://dx.doi.org/10.9713/kcer.2014.52.4.497

Optimization of Adsorbent Treatment Process for the Purification of Paclitaxel from Plant Cell Cultures of Taxus chinensis  

Lee, Chung-Gi (Department of Chemical Engineering, Kongju National University)
Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
Publication Information
Korean Chemical Engineering Research / v.52, no.4, 2014 , pp. 497-502 More about this Journal
Abstract
Biomass-derived tar and waxy compounds have a highly negative effect on the separation and purification of paclitaxel and should be removed prior to final purification. Adsorbent treatment is a simple, efficient method for removal of tar and waxy compounds from plant cell cultures. In this study, we optimized the important process parameters (adsorption temperature, time, solvent type and adsorbent amount) of adsorbent treatment with Sylopute to remove the tar and waxy compounds in a pre-purification step. The optimal adsorption temperature, adsorption time, solvent type, and crude extract/Sylopute ratio were $30^{\circ}C$, 15 min, methylene chloride, and 1:1(w/w), respectively. This result could be confirmed by HPLC analysis of the absorbent after treatment and TGA of the organic substances that were bound to the adsorbent. In adsorbent treatment step, the purity seemed to show a small improvement but this treatment had a significant effect on convenience and feasibility of following steps by the removal of tar and waxy compounds.
Keywords
Paclitaxel; Adsorbent Treatment; Process Parameter; Optimization; Sylopute;
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Times Cited By KSCI : 3  (Citation Analysis)
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