• 제목/요약/키워드: Swelling effect

검색결과 771건 처리시간 0.023초

Effect of Monothioglycerol on ROS Inhibition, Mitochondrial Activity, and DNA Integrity in Frozen-thawed Miniature Pig Sperm (Monothiolglycerol이 동결 융해 후 미니돼지 정자의 활성산소 억제, 미토콘드리아 활성 그리고 DNA Integrity에 미치는 영향)

  • Park, Soo-Jung;Kim, Dae-Young
    • Journal of Embryo Transfer
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    • 제28권3호
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    • pp.265-271
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    • 2013
  • Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG $0.2{\mu}M$, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.

Changes of Recycled Paper Properties and Waste Paper Deinkability by the Repeated Recycling of Photocopy Paper (복사용지의 리사이클링 반복처리에 따른 재생지의 특성 및 고지의 탈묵성 변화)

  • Shin, Jun-Seop
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • 제4권2호
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    • pp.24-32
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    • 1998
  • This study examined the effects of recycling on chemical pulp fibers from multiple recycles. Firstly, water-pretreated alkaline photocopy paper was disintegrated by TAPPI standard disintegrator at room temperature. After dewatering, this pulp was dried in oven at $80^{\circ}C$ for 24hrs. A sequence of wetting, disintegrating and drying was one recycling cycle and this cyclic treatment was repeated from zero to five times. The recycled handsheet dropped to 90% of the original brightness after five cycles, and lost the most brightness in the first two cycles. However, it had a gain of 10% in opacity after five cycles. And, in this study, the method for determining residual ink(toner) content in recycled handsheets were estabilished by means of SEM-EDX and atomic absorptive photometer. The change of residual ink percentage on recycled paper showed the effect of recycling numbers on deinkability of waste paper. A slight decrease in deinkability was noted for the recycled handsheets, which may be due to the change of fiber surface free energy connected with fiber swelling.

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Effects of Fiber Wall Thickness on Paper Properties Using CLSM (CLSM을 이용한 고해과정 중 섬유벽 두께 변화의 종이 특성 영향 분석)

  • 김서환;박종문;김철환
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • 제31권1호
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    • pp.39-45
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    • 1999
  • Refining in papermaking plays an important role in changing fiber properties as well as paper properties. The major effects of refining on pulp fibers are internal and external fibrillation, fiber shortening, and fines formation. Many workers showed that internal fibrillation of the primary refining effects was most influential in improving paper properties. In particular, refining produces separation of fiber walls into several lamellae, thus causing fiber wall swelling with water penetration. This leads to the increase of fiber flexibility and of fiber-to-fiber contact during drying. If the fibers are very flexible, they will be drawn into close contact with each other by the force of surface tension as the water is removed during the drainage process and drying stages. In order to study the effect of fiber wall delamination on paper properties, cross-sectional image of fibers in a natural condition had to be generated without distortion. Finally, it was well recognized that confocal laser scanning microscope (CLSM) could be one of the most efficient tool for creating and quantifying fiber wall delamination in combination with image analysis technique. In this study, the CLSM could be used not only to observe morphological features of transverse views of swollen fibers refined under low and high intensity, but also to investigate the sequence of fiber wall delamination and fiber wall breakage. From the CLSM images, increasing the specific energy or refining decreased the degree of fiber collapse, fiber cross-sectional area, fiber wall thickness and lumen area. High intensity refining produced more external fibrillation.

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Treatment of Deep Venous Thromboses of Lower Leg with Thrombolysis (혈전용해술을 이용한 하지 심부정맥 혈전증의 치료)

  • 이재원
    • Journal of Chest Surgery
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    • 제34권9호
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    • pp.711-715
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    • 2001
  • Background: Deep venous thrombosis(DVT) is a curable disease when it is appropriately treated in the early stages of onset. The long term follow up of chronic DVT shows poor prognosis with serious complications such as venous valvular insufficiency, venous claudication, venous ulcer and leg swelling. Thrombolytic therapy is a very active treatment that delivers thrombolytic agents via catheter to the target thrombi. The aim of this study is to evaluate the effect of catheter directed thrombolysis using urokinase to acute DVT. Material and Method: We studied 5 patients, who were diagnosed as acute DVT and had no contraindication for selective hemolysis using urokinase. Result: All the patients were successfully recanalized. Total infusion time of urokinase was 2.0$\pm$0.6 days, and the amount was 5.9$\pm$2.45 million IU. In 4 patients, who were diagnosed as May-Therner syndrome, we performed the balloon angioplasty and inserted the stent at the stenotic portion. There were minor complications such as hematuria, hematoma at puncture site, and all of them are self limited. Conclusion: Catheter induced thrombolysis is an effective treatment in acute DVT.

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Studies on the Toxigenic Fungi and Detoxification Methods Isolated from Korean Foodstuffs (한국(韓國) 식품중(食品中)의 유독성(有毒性) 진균(眞菌)의 검색(檢索) 및 해독(解毒)에 관(關)한 연구(硏究))

  • Koh, Choon-Myung;Lee, Bong-Kee;Kim, Se-Jong;Lew, Joon
    • The Journal of the Korean Society for Microbiology
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    • 제12권1호
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    • pp.33-49
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    • 1977
  • These studies were carried out to detect the presence of mycotoxin fungi and detoxification of toxins in various kinds of grain and foodstuffs in Korea. The experiments were divided into three parts: mycologic, toxicologic and electron microscopic study. The resuIts were summarized as follows: 1. From the 210 various, samples(local grains, 150 samples; rice-cakes, 50 samples; meju, 10 samples), 1,205 colonies or fungi were isolated. In 1,127 of 1,205 colonies, it was possible to identify 21 genera. Among the identified strains, the predominant genera were Aspergillus sp.(28.5%), Penicillium sp.(27.1%) and Mucor sp.(7.8%). 2. In cytotoxicity test on HeLa cells, 25 strains showed severe toxic effects among the 240 strains of experiments. 3. In histopathologic test on mice, 21 strains showed severe toxic effect to mouse liver cells among the 240 tested strains. 4. In electron microscopic studies of HeLa cells and mouse liver cells from animal which had been treated with crude toxin, the liver cells showed the cytoplasmic change: dillatation or vacuolization of endoplasmic reticulum, swelling of mitochondria and disappearance of mitochondrial cristae, increased number of lipid and glycogen particles. Nucleus and nucelar envelope alterations, were also noted. 5. In the detoxification study with ultraviolet irradiation, 90% of the toxic substances were denatured by the ultraviolet light irradiation for 24 hours. 6. As a mass screening, the cytotoxicity test of HeLa cells and histopathologic study of mice liver cells treated with culture filtrates, employed and feasible to detect mycotoxin producing fungi.

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Experimental and modelling study of clay stabilized with bottom ash-eco sand slurry pile

  • Subramanian, Sathyapriya;Arumairaj, P.D.;Subramani, T.
    • Geomechanics and Engineering
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    • 제12권3호
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    • pp.523-539
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    • 2017
  • Clay soils are typical for their swelling properties upon absorption of water during rains and development of cracks during summer time owing to the profile desorption of water through the inter-connected soil pores by water vapour diffusion leading to evaporation. This type of unstable soil phenomenon by and large poses a serious threat to the strength and stability of structures when rest on such type of soils. Even as lime and cement are extensively used for stabilization of clay soils it has become imperative to find relatively cheaper alternative materials to bring out the desired properties within the clay soil domain. In the present era of catastrophic environmental degradation as a side effect to modernized manufacturing processes, industrialization and urbanization the creative idea would be treating the waste products in a beneficial way for reuse and recycling. Bottom ash and ecosand are construed as a waste product from cement industry. An optimal combination of bottom ash-eco sand can be thought of as a viable alternative to stabilize the clay soils by means of an effective dispersion dynamics associated with the inter connected network of pore spaces. A CATIA model was created and imported to ANSYS Fluent to study the dispersion dynamics. Ion migration from the bottom ash-ecosand pile was facilitated through natural formation of cracks in clay soil subjected to atmospheric conditions. Treated samples collected at different curing days from inner and outer zones at different depths were tested for, plasticity index, Unconfined Compressive Strength (UCS), free swell index, water content, Cation Exchange Capacity (CEC), pH and ion concentration to show the effectiveness of the method in improving the clay soil.

A Study of Queen Elizabeth I's Cinema Costumes Viewed from Her Portrait (초상화 복식을 중심으로 본 퀸 엘리자베스 1세의 영화 의상에 관한 연구)

  • Sung, Kwang Sook
    • Journal of the Korean Society of Costume
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    • 제62권8호
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    • pp.55-70
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    • 2012
  • In 16th century, during renaissance time, Britain's queen Elizabeth I, as a means to express her authority, had an exaggerated clothing styles and was the fashion leader of the time. Thus, through a media of Cinema, tried to find out the characteristics and different aspects of the history of clothing styles, of how they changed, ascertained, restored, and reappeared in the modern time. The research was based on the consideration of Elizabeth I's 33 portraits and her clothing styles; and the research is also from analysing the movies from 1937 to 2007, a total of 9 Cinema and 102 clothes. Movie costumes' most important mission is representing the cinema's timely and spatial background. Thus, it is inevitable that ascertaining and restoring Elizabeth I's portrait should be done. However, the movie costume has to show her dramatic lifestyle, dignity, absolute authority, and other visual images. In addition, the costume has to contribute in making artistic effect and thus the creativity of a designer to satisfy all these needs should be added. Based on the findings from the research, the characteristics of Elizabeth I's Cinema costume are first, ascertaining and restoring portraits, second, mixture of silhouette and detail depending on time period, third, partial change on clothing style design, fourth, emphasis of ruff collar, fifth, magnification colors, sixth, avoiding excessive swelling and decoration, and applying modern style on Protective costume. Thus, based on the research, creative design in Cinema clothing, plays an important part in the history of clothing design. I hope this research could work as a foundation reference for clothing design's history.

Effect of Pioglitazone on Perihematomal Edema in Intracerebral Hemorrhage Mouse Model by Regulating NLRP3 Expression and Energy Metabolism

  • Kim, Hoon;Lee, Jung Eun;Yoo, Hyun Ju;Sung, Jae Hoon;Yang, Seung Ho
    • Journal of Korean Neurosurgical Society
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    • 제63권6호
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    • pp.689-697
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    • 2020
  • Objective : Cerebral edema is the predominant mechanism of secondary inflammation after intracerebral hemorrhage (ICH). Pioglitazone, peroxisome proliferator-activated receptor gamma agonist has been shown to play a role in regulation of central nervous system inflammation. Here, we examined the pharmacological effects of pioglitazone in an ICH mouse model and investigated its regulation on NLRP3 inflammasome and glucose metabolism. Methods : The ICH model was established in C57 BL/6 mice by the stereotactical inoculation of blood (30 µL) into the right frontal lobe. The treatment group was administered i.p. pioglitazone (20 mg/kg) for 1, 3, and 6 days. The control group was administered i.p. phosphate-buffered saline for 1, 3, and 6 days. We investigated brain water contents, NLRP3 expression, and changes in the metabolites in the ICH model using liquid chromatography-tandem mass spectrometry. Results : On day 3, brain edema in the mice treated with pioglitazone was decreased more than that in the control group. Expression levels of NLRP3 in the ICH model treated with pioglitazone were decreased more than those of the control mice on days 3 and 7. The pioglitazone group showed higher levels of glycolytic metabolites than those in the ICH mice. Lactate production was increased in the ICH mice treated with pioglitazone. Conclusion : Our results demonstrated less brain swelling following ICH in mice treated with pioglitazone. Pioglitazone decreased NLRP3-related brain edema and increased anaerobic glycolysis, resulting in the production of lactate in the ICH mice model. NLRP3 might be a therapeutic target for ICH recovery.

Study on the Protective Effects of 6R-Tetrahydrobiopterin on the Oxidative Neuronal Injury in Mouse Cortical Cultures (배양된 대뇌피질세포에서 산화성 손상에 대한 6R-Tetrahydrobiopterin의 억제작용)

  • Moon, Kyung Sub;Lee, Je Hyuk;Kang, Sam Suk;Kim, Soo Han;Kim, Jae Hyoo;Jung, Shin;Kim, Tae Sun;Lee, Jung Kil
    • Journal of Korean Neurosurgical Society
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    • 제30권9호
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    • pp.1059-1064
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    • 2001
  • Objective : 6R-Tetrahydrobiopterin(BH4) is a cofactor for the aromatic amino acid hydroxylases which is essential for the biosynthesis of catecholamines and serotonin. It also acts as a cofactor for nitric oxide synthase, and stimulates the release of some neurotransmitters such as dopamine, serotonin, acetylcholine and glutamate. Recently, it has been reported that BH4 could induce cellular proliferation and enhance neuronal survival. This study was performed to investigate the antioxidative effect of BH4 on the various oxidative insults in mouse cerebral cortical cell cultures. Methods : Iron ion(FeCl2), zinc ion(ZnCl2), sodium nitroprusside(SNP) and buthionine sulfoximine(BSO, a glutathione depletor) were used as oxidants. Cell death was assessed by measurement of lactate dehydrogenase efflux to bathing media at the end of exposure. Result : All 4 oxidants induced neuronal cell death associated with cell body swelling, which was markedly inhibited by trolox($100{\mu}M$), a vitamin E analog. BH4($10-100{\mu}M$) markedly inhibited the neuronal cell death induced by all 4 oxidants($20{\mu}M\;Cu^{2+}$, $20{\mu}M\;Zn^{2+}$, $1{\mu}M$ SNP or 1mM BSO). However, BH4 failed to inhibit the neuronal cell death induced by 24hr exposure to $20{\mu}M$ NMDA. Conculsion : These results suggest that BH4 has antioxidative action independently of any actions of enzyme cofactor.

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Prescriptions Utilizing Vegetable Oils in 『Donguibogam』(『東醫寶鑑』) (『동의보감(東醫寶鑑)』중 식물성 기름(油)을 활용한 방제)

  • Lee, Boo-Kyun
    • Herbal Formula Science
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    • 제28권3호
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    • pp.301-311
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    • 2020
  • Object : To investigate which diseases the utilization of various vegetable oils were used and how they were used for treatment, and to change and develop vegetable oil combined prescriptions so that the Korean medical treatment effect in clinical practice can be enhanced. Methods : All words containing oil(油) were searched and selected oils used for simple prescription and oil combined prescription. Aii of animal oils such as butter(酥油), Suis oil(猪油), Anceris oil(鴈油), and Actonis caro oil(猯油) were excludIt is suggested to develop a new formulation by succeeding the utilization methods of various effects of sesame oil and other vegetable oils.ed. All selected oils were classified as sesame oil(麻油)(inclusive, 脂麻油, 胡麻油, 香油, 淸油) or other vegetable oils. Result : 1. In 『Donguibogam』(『東醫寶鑑』), 14 other vegetable oils were used to 23 prescriptions. 2. Sesame oil was used to 187 prescriptions over 41 disease categories as of 20 effects described in the 『Zhenghebencao』(『政和本草』). 3. In 28 prescriptions, sesame oil was also used for hygiene and prevention, emetic, preparating herbs and so on. Conclusion : The 101 prescriptions used for medicinal auxiliary materials are due to the efficacy of treatments of malignant furuncles, sores, scabies, promoting regeneration of tissue and flesh, reliving pain, dispersing abscess and swelling and supplementing fissured skin. In 『Donguibogam』(『東醫寶鑑』), the unique efficacy of each vegetable oil and sesame oil was used in various ways and preparation forms to treat various diseases. It is suggested to develop a new formulation by succeeding the utilization methods of various effects of sesame oil and other vegetable oils.