• 미생물학회지
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• 제53권1호
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• pp.64-66
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• 2017

Caballeronia sordidicola strain PAMC 26592 was isolated from Umbilicaria sp., a lichen material collected from Svalbard Archipelago in the Arctic Ocean. We report the draft genome sequence of the strain PAMC 26592, a metabolic generalist. As we have observed in previous genomic studies in the genus Caballeronia draft genomic sequences of PAMC 26592 had an assortment of genes of ecological importance and of bio-technical potentials, which include diverse metabolic genes for carbohydrates, aromatic compounds, amino acids, and vitamins, and genes for nitrogen / sulfur metabolisms, stress responses, membrane transporters, antibiotic resistance, and heavy metal resistance.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1275-1283
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• 2022

Understanding soil microbial community structure in the Arctic is essential for predicting the impact of climate change on interactions between organisms living in polar environments. The hypothesis of the present study was that soil microbial communities and soil chemical characteristics would vary depending on their associated plant species and local environments in Arctic mature soils. We analyzed soil bacterial communities and soil chemical characteristics from soil without vegetation (bare soil) and rhizosphere soil of three Arctic plants (Cassiope tetragona [L.] D. Don, Dryas octopetala L. and Silene acaulis [L.] Jacq.) in different local environments (coal-mined site and seashore-adjacent site). We did not observe any clear differences in microbial community structure in samples belonging to different plant rhizospheres; however, samples from different environmental sites had distinct microbial community structure. The samples from coal-mined site had a relatively higher abundance of Bacteroidetes and Firmicutes. On the other hand, Acidobacteria was more prevalent in seashore-adjacent samples. The relative abundance of Proteobacteria and Acidobacteria decreased toward higher soil pH, whereas that of Bacteroidetes and Firmicutes was positively correlated with soil pH. Our results suggest that soil bacterial community dissimilarity can be driven by spatial heterogeneity in deglaciated mature soil. Furthermore, these results indicate that soil microbial composition and relative abundance are more affected by soil pH, an abiotic factor, than plant species, a biotic factor.

• Ocean and Polar Research
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• 제26권1호
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• pp.51-58
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• 2004

We isolated and identified culturable Arctic bacteria that had inhabited soils around the Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway $(79^{\circ}N,\;12^{\circ}E)$. The collected soils were diluted in distilled water; the diluted soil-water was spread on 3M petri-films at Dasan Station. The petri-films were transported to the laboratory at KORDI, and cultured at $4^{\circ}C$. Colonies grown on the petri-films were subsequently cultured on nutrient agar plates at $4^{\circ}C$ every 7 days. The pure colonies were inoculated into nutrient liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 165 rDNA sequences. A total of 227 strains of bacteria were isolated. Among them, 16S rDNA sequences of 185 strains were identical with those of known strains isolated in this study, and 42 strains were finally identified. Phylogenetic analysis using 16S rDNA indicated that the 30 strains belonged to Pseudomonas, 7 strains to Arthrobacter, two strains to Flavobacterium, and the remaining to Achromobacter, Pedobacter, and Psychrobacter. Among the 42 strains, 14 bacteria produced protease: they were 6 strains of Pseudomonax, 4 strains of Arthrobater, an Achromobacter strain, 2 strains of Flavobacterium, and a Pedohacter strain. We expect these Arctic bacteria can be used for screening to develop new industrial enzymes that are active at low temperatures.

• 한국위성정보통신학회논문지
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• 제10권3호
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• pp.102-108
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• 2015

다목적실용위성 2호/3호/5호는 한반도 및 해외영상에 대한 수요증가로 인해서 위성의 영상촬영 횟수가 지속적으로 증가하고 있으며, 촬영이후 빠른 시간 내에 영상을 획득하고자 하는 요구가 많아지고 있다. 이러한 수요를 만족하기 위해서 국내 지상국뿐 아니라 해외 지상국에 대한 사용 빈도가 높아지고 있다. 한국항공우주연구원에서는 노르웨이 스발바르에 다목적실용위성 2호와 3호의 수신시스템을 운영하고 하루 4~6회 이상 수신하고 있으며, 5호와 3A호의 해외수신을 계획하고 있다. 본 논문에서는 한국항공우주연구원에서 운영하고 있는 노르웨이 극지수신 시스템에 대해 설명하고 다중위성 운영을 대비한 극지 통합수신시스템에 대한 설계 및 개발과정에 대하여 설명한다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 춘계학술대회 논문집
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• pp.210-215
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• 2007

북극 다산기지가 있는 니알슨 인근 해역인 Kongsfjorden만은 일부 해역이 해빙으로 덮혀 있는 반폐쇄적인 만으로 수심이 평균 200m 이상 된다. 이곳 북극 지방은 하절기 라도 태양의 고도가 낮아 해색위성분야의 극지활용에 대한 가능성 여부도 명확하게 알려져 있지 못하다. 따라서 본 연구에서는 북극 다산기지가 위치한 스발바드 군도의 니알슨 주변 해역(Kongsfjorden 해역)을 중심으로 2006년 8월 현장관측된 자료들을 유사한 시기의 한반도 주변 해역에서 현장관측된 자료들과 비교/분석하여 해양환경적 특성과 해수광학적인 특성이 어떤 차이가 있는지를 비교 분석하였다. 또한 북극 지방에서 인공위성을 이용한 해색위성자료의 극지활용이 가능한지를 검토하였다. 해수의 광 특성으로, 니알슨 주변 해역의 ${\alpha}^*_{ph}(440)$ 최대값은 $0.1m^2/mg$으로 한반도 주변해수의 ${\alpha}^*_{ph}(440)$ 최대값보다 약 2배정도 높게 측정되어 플랑크톤의 광 흡수효율이 중위도보다 상대적으로 높은 것으로 분석되었다. ${\alpha}^*_{ss}(440)$ 값은 $0.04-0.1m^2/g$으로 동중국해와 유사하며，한반도 주변해수보다 높은 값을 나타냈다. 이것은 Kongsfjorden 해역의 부유물질(SS) 구성성분이 동중국해와 유사한 무기광물 입자로 주로 구성되어 있음을 보여주고 있다. ${\alpha}_{dom}(400)$ 값은 $0.08-0.25m^{-1}$로 한반도 주변해수의 ${\alpha}_{dom}(400)$이 값과 유사하고 연안해역의 ${\alpha}_{dom}(400)$ 값보다 낮은 값을 나타냈다. $E_d(460)$의 평균값은 Konsfjorden 해역 $40mW/cm^2/{\mu}m$, 동중국해 $120mW/cm^2/{\mu}m$, 황해남부 $110mW/cm^2/{\mu}m$, 남해연안 $100mW/cm^2/{\mu}m$, 진해만 $100mW/cm^2/{\mu}m$이었다. 이와 같은 현장관측결과를 토대로 분석한 북극 다산기지 주변 해역의 해수광학적인 환경 특성은 용존유기물의 흡광도를 기준으로 볼 때 대양(Open Sea)의 광 특성과 유사하였다. 또한 북극지방에서 태양의 해수 입사 광량은 중위도의 약 40%정도이지만 해색위성자료의 극지활용이 가능하다고 판단되었다.

• Ocean and Polar Research
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• 제31권2호
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• pp.213-218
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• 2009

We examined the intracellular antioxidative effects of 20 Arctic seaweed extracts in Raw 264.7 cells. Each seaweed species was subjected to extraction using acetone/dichloromethane and methanol, respectively, after which the extracts were combined and used as the test sample. The antioxidant ability of all 20 seaweeds extracts was evaluated using four different activity tests, including the degree of occurrence of intracellular reactive oxygen species (ROS), $ONOO^-$, and lipid peroxidation in Raw 264.7 cells, as well as the extent of oxidative damage of genomic DNA purified from Raw 264.7 cells. Crude extracts from Monostroma obscurum, Alaria esculnta, Laminaria digitata, Desmarestia aculeata, Chorda filum, Ptilota seriata, Phycidrys rubens, Devaleraea ramentacea and Palmaria palmata exhibited significant scavenging effects on the generation of intracellular ROS. Among them, Monostroma obscurum and Phycidrys rubens significantly inhibited membrane lipid peroxidation and DNA oxidation. Moreover, Phycidrys rubens exhibited scavenging effects on peroxynitrite generated from SIN-1.

• ALGAE
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• 제31권1호
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• pp.61-72
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• 2016

Arctic microalgae thrive and support primary production in extremely cold environment. Three Arctic green microalgal strains collected from freshwater near Dasan Station in Ny-Alesund, Svalbard, Arctic, were analyzed to evaluate the optimal growth conditions and contents of fatty acids. The optimal growth temperature for KNF0022, KNF0024, and KNF0032 was between 4 and 8℃. Among the three microalgal strains, KNF0032 showed the maximal cell number of 1.6 × 107 cells mL^-1 at 4℃. The contents of fatty acids in microalgae biomass of KNF0022, KNF0024, and KNF0032 cultured for 75 days were 37.34, 73.25, and 144.35 mg g^-1 dry cell weight, respectively. The common fatty acid methyl esters (FAMEs) analyzed from Arctic green microalgae consisted of palmitic acid methyl ester (C16:0), 5,8,11-heptadecatrienoic acid methyl ester (C17:3), oleic acid methyl ester (C18:1), linoleic acid methyl ester (C18:2), and α-linolenic acid methyl ester (C18:3). KNF0022 had high levels of heptadecanoic acid methyl ester (26.58%) and heptadecatrienoic acid methyl ester (22.17% of the total FAMEs). In KNF0024 and KNF0032, more than 72.09% of the total FAMEs consisted of mono- and polyunsaturated fatty acids. Oleic acid methyl ester from KNF0032 was detected at a high level of 20.13% of the FAMEs. Arctic freshwater microalgae are able to increase the levels of polyunsaturated fatty acids under a wide range of growth temperatures and can also be used to produce valuable industrial materials.

• 미생물학회지
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• 제53권2호
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• pp.141-143
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• 2017

Caballeronia sordidicola 균주 PAMC 26577은 다산 기지 근처에서 채집된 지의류인 Cladonia 종에서 분리되었다. Illumina 방식으로 분석한 균주 PAMC 26577의 초안 유전체 서열은 182개의 콘티그로 이루어졌으며, N50값은 159,226 염기쌍 길이에 해당하였다. 초안 유전체로 총 8,334,211 염기쌍을 확인하였으며, 59.4% G+C 함량을 나타냈다. 유전체는 단백질을 코드하지 않는 8개의 rRNA 유전자와 51개의 tRNA 유전자를 포함하였다. 8,065개의 단백질 유전자는 기본 대사 과정뿐 아니라 부탄올/부티르산 생합성, 폴리하이드록시부티르산 대사, serine cycle methylotrophy 및 글라이코겐 대사 유전자들을 가지고 있었다. 2백개 이상의 막 전달 단백질은, 인산화 전달 시스템과 TRAP 전달시스템이 부재하였다. PAMC 26577은 CRISPR 관련 서열 및 단백질이 없었으며, 파아지 유전자의 감염흔적으로 인한 11개의 파아지 관련 유전자를 찾아낼 수 있었다.

• Journal of Ecology and Environment
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• 제46권4호
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• pp.282-291
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• 2022

Background: The Arctic permafrost stores enormous amount of carbon (C), about one third of global C stocks. However, drastically increasing temperature in the Arctic makes the stable frozen C stock vulnerable to microbial decomposition. The released carbon dioxide from permafrost can cause accelerating C feedback to the atmosphere. Soil organic matter (SOM) composition would be the basic information to project the trajectory of C under rapidly changing climate. However, not many studies on SOM characterization have been done compared to quantification of SOM stocks. Thus, the purpose of our study is to determine soil properties and molecular compositions of SOM in four different Arctic regions. We collected soils in different soil layers from 1) Cambridge Bay, Canada, 2) Council, Alaska, USA, 3) Svalbard, Norway, and 4) Zackenberg, Greenland. The basic soil properties were measured, and the molecular composition of SOM was analyzed through pyrolysis-gas chromatography/mass spectrometry (py-GC/MS). Results: The Oi layer of soil in Council, Alaska showed the lowest soil pH and the highest electrical conductivity (EC) and SOM content. All soils in each site showed increasing pH and decreasing SOC and EC values with soil depth. Since the Council site was moist acidic tundra compared to other three dry tundra sites, soil properties were distinct from the others: high SOM and EC, and low pH. Through the py-GC/MS analysis, a total of 117 pyrolysis products were detected from 32 soil samples of four different Arctic soils. The first two-axis of the PCA explained 38% of sample variation. While short- and mid-hydrocarbons were associated with mineral layers, lignins and polysaccharides were linked to organic layers of Alaska and Cambridge Bay soil. Conclusions: We conclude that the py-GC/MS results separated soil samples mainly based on the origin of SOM (plants- or microbially-derived). This molecular characteristics of SOM can play a role of controlling SOM degradation to warming. Thus, it should be further investigated how the SOM molecular characteristics have impacts on SOM dynamics through additional laboratory incubation studies and microbial decomposition measurements in the field.

• Ocean and Polar Research
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• 제45권2호
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• pp.89-102
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• 2023

Climate and environmental changes in the Arctic Ocean due to global warming have been linked to extreme climate change in mid-latitude regions, including the Korean Peninsula, requiring a better understanding of the Arctic climate system based on the paleo-analog. This review introduces three paleoenvironmental research cases using neodymium isotopes (¹⁴³Nd/¹⁴⁴Nd, ε_Nd) measured on two different fractions of marine sediments: silicate-bound 'detrital' and Fe-Mn oxide-dominated 'authigenic' fractions. In the first case, detrital ε_Nd in core HH17-1085-GC on the continental shelf off northern Svalbard was used for tracing changes in sediment provenance and associated glacier behavior over the last 16.3 ka. The second case showed the potential use of authigenic ε_Nd as a quasi-conservative water mass tracer. Three prominent ε_Nd peaks and troughs observed in core PS72/410-1 from the Mendeleev Ridge in the western Arctic Ocean over the past 76 ka suggested episodic meltwater discharge events during 51~46, 39~35 and 21~13 ka BP. The last case proposed the use of the difference between authigenic and detrital ε_Nd as a proxy for reconstructing glacier fluctuation. The idea is based on the assumption that enhanced glacial erosion during glacier advances can supply sufficient freshly-exposed rock substrate for incongruent weathering, potentially leading to greater isotopic decoupling between bedrock and dissolved weathering products as recorded in detrital and authigenic ε_Nd, respectively. Thus, it would be worthwhile to take advantage of sedimentary ε_Nd to improve our understanding of past environmental changes in polar regions.