• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1673-1680
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• 2012

In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride ($DEAE{\cdot}HCl$)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized $DEAE{\cdot}HCl$ derivatization and adsorption conditions between yeast cells and the DEAE-corncobs. When delignified corncob grit (3.0 g) was derivatized with 0.5M $DEAE{\cdot}HCl$, the yeast cell suspension ($OD_{600}$ = 3.0) was adsorbed at >90% of the initial cell $OD_{600}$. This amount of adsorbed yeast cells was estimated to be 5.36 mg-dry cells/g-DEAE corncobs. The $Q_{max}$ (the maximum cell adsorption by the carrier) of the DEAE-corncobs was estimated to be 25.1 (mg/g), based on a Languir model biosorption isotherm experiment. When we conducted a batch culture with medium recycling using the immobilized yeast cells, the yeast cells on DEAE-corncobs produced ethanol gradually, according to glucose consumption, without cells detaching from the DEAE-corncobs. We observed under electron microscopy that the yeast cells grew on the surface and in the holes of the DEAE-corncobs. In a future study, DEAE-corncobs and the immobilized yeast cell reactor system will contribute to bioethanol production from biomass hydrolysates.

• 한국산학기술학회논문지
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• 제14권1호
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• pp.57-62
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• 2013

카트 차체에는 현가장치와 차동장치가 존재하지 않으므로 카트주행 중 발생하는 프레임의 변형은 탄성변형으로 인한 주행성능과 프레임의 피로수명에 영향을 미친다. 선회주행 시 비틀림 변형에 의한 카트의 거동은 이 두 가지 변형에 결정적 원인을 제공한다. 이러한 곡선구간에서 카트의 동적 거동을 분석하기 위하여 GPS를 이용하여 차량의 궤적을 추적하고 카트 프레임 강에 작용하는 비틀림 응력을 측정하였다. 레저와 레이싱 카트 프레임의 기계적 성질을 파악하기 위해 재질 분석과 인장시험을 실시하여 각 프레임의 재료 특성을 분석하였다. 비틀림 응력집중과 프레임 비틀림은 프레임 해석을 통해 조사되었다. 또한 주행 분석 장치를 이용하여 레저와 레이싱 카트를 각 조건별로 실차실험을 수행하였고, 이를 통해 곡선구간에서 카트의 주행거동을 분석하였다. 선회주행 시 차량에 원심력에 의한 하중이동의 현상이 발생했으며, 이로 인해 카트 프레임 강에 비틀림 응력이 발생하였다.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1173-1180
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• 1997

Hepatotoxicity of caffeine and acetaminophen was investigated in this study. Special attention was paid to the effect of vitamins on the reduction of hepatotoxicity caused by the chemicals. Rat hepaocytes isolated by two-step perfusion method were cultured in two differents methods-suspension, monolayer cultures-, and exposed to caffeine and/or acetaminophen for 24hrs. Caffeine or acetaminophen exhibited no significant hepatotoxicity in terms of intracellular glutathione(GSH) level and lipid peroxidation(MDA), but GSH level was significantly decreased after administrated acetaminophen, and the toxicity caused by the chemicals showed a dose-dependent manner. The synergistic effect of caffeine and acetaminophen was observed when both caffeine and acetaminophen were supplemented to culture medium. At the concentration 1mM, caffeine enhanced the intracellular GSH depletion and MDA formation by 63% and 64%, respectively, compared to single supplementation of 10mM acetaminophen in culture medium. This hepatotoxicity induced membrane integrity loss was observed by lightmicroscope on the simultaneous administration of caffeine and acetaminophen in monolayer cultured hepatocytes. Co-supplementation of vitamins with caffeine/acetaminophen to culture medium results in the protection of hepatocytes from hepatotoxic attach by caffeine/acetaminophen. Especially, vitamin E was superior to vitamin C and $\beta$-carotene from the standpoints of GSH depletion and MDA formation. From this results, it has been speculated that vitamin E may play a role of antioxidant scavenging radicals produced from acetaminophen. Taken all together, in vitro culture system like monolayer culture of hepatocytes may be a useful tool for the evaluation of hepatotoxicity or protection ability of food ingredients.

• 비교교육연구
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• 제22권4호
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• pp.25-49
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• 2012

미국에서는 1980년대 이후 학교폭력, 무기사용, 마약복용 등의 문제에 대응하기 위하여 무관용정책(zero tolerance)을 시행하고 있다. 학교폭력이나 비행이 발생한 경우 미리 예정된 정학 또는 퇴학을 엄격하게 적용하여 학교의 안전과 학습 분위기 개선을 도모하고 있다. 실제로 학교폭력비율이 감소하고 있는 것으로 나타났다. 그러나 다른 한편으로 사소한 비행에 대해서도 엄격한 처벌을 함으로써 비행이 더 악화되며, 효용성도 증명되지 않는다는 비판이 제기되었다. 이에 대안으로 무관용정책의 대원칙은 유지하면서도 폭력의 정도에 따라 처벌의 정도를 달리하자는 등급징계제도 또는 조기반응모형이 제시되고 있다. 최근 우리나라에서도 학교폭력이 사회적 이슈로 강하게 부각됨에 따라 정부도 '학교폭력근절 종합대책'을 발표하는 등 범부처 차원에서 대응하고 있다. 이 논문은 사소한 괴롭힘도 '범죄'라는 인식하에 철저히 대응하는 정부의 방침의 타당성을 탐색하기 위해 현재 미국에서 시행하고 있는 학교폭력에 대한 무관용 정책의 주요 내용과 변화과정을 분석하여 정책적 시사점을 도출하는 데 목적이 있다.

• Wind and Structures
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• 제23권3호
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• pp.211-229
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• 2016

In recent years, the frequency and duration of supply interruption in electric power transmission system due to flashover increase yearly in China. Flashover is usually associated with inadequate electric clearance and often takes place in extreme weathers, such as downbursts, typhoons and hurricanes. The present study focuses on the wind-induced oscillation of conductor during the process when a downburst is passing by or across a specified transmission line. Based on a revised analytical model recently developed for stationary downburst, transient three-dimensional wind fields of moving downbursts are successfully simulated. In the simulations, the downbursts travel along various motion paths according to the certain initial locations and directions of motion assumed in advance. Then, an eight-span section, extracted from a practical 500 kV ultra-high-voltage transmission line, is chosen. After performing a non-linear transient analysis, the transient displacements of the conductors could be obtained. Also, an extensive study on suspension insulator strings' rotation angles is conducted, and the electric clearances at different strings could be compared directly. The results show that both the variation trends of the transient responses and the corresponding peak values vary seriously with the motion paths of downburst. Accordingly, the location of the specified string, which is in the most disadvantageous situation along the studied line section, is picked out. And a representative motion path is concluded for reference in the calculation of each string's oscillation for the precaution of wind-induced flashover under downburst.

• Safety and Health at Work
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• 제6권4호
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• pp.268-278
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• 2015

Background: This study aimed to assess the whole-body vibration (WBV) exposure among large blast hole drill machine operators with regard to the International Organization for Standardization (ISO) recommended threshold values and its association with machine- and rock-related factors and workers' individual characteristics. Methods: The study population included 28 drill machine operators who had worked in four opencast iron ore mines in eastern India. The study protocol comprised the following: measurements of WBV exposure [frequency weighted root mean square (RMS) acceleration ($m/s^2$)], machine-related data (manufacturer of machine, age of machine, seat height, thickness, and rest height) collected from mine management offices, measurements of rock hardness, uniaxial compressive strength and density, and workers' characteristics via face-to-face interviews. Results: More than 90% of the operators were exposed to a higher level WBV than the ISO upper limit and only 3.6% between the lower and upper limits, mainly in the vertical axis. Bivariate correlations revealed that potential predictors of total WBV exposure were: machine manufacturer (r = 0.453, p = 0.015), age of drill (r = 0.533, p = 0.003), and hardness of rock (r = 0.561, p = 0.002). The stepwise multiple regression model revealed that the potential predictors are age of operator (regression coefficient ${\beta}=-0.052$, standard error SE = 0.023), manufacturer (${\beta}=1.093$, SE = 0.227), rock hardness (${\beta}=0.045$, SE = 0.018), uniaxial compressive strength (${\beta}=0.027$, SE = 0.009), and density (${\beta}=-1.135$, SE = 0.235). Conclusion: Prevention should include using appropriate machines to handle rock hardness, rock uniaxial compressive strength and density, and seat improvement using ergonomic approaches such as including a suspension system.

• Journal of Biosystems Engineering
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• 제43권4호
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• pp.394-400
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• 2018

Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of $30{\mu}L$ of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at $877.7cm^{-1}$. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

• 한국구조물진단유지관리공학회 논문집
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• 제23권5호
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• pp.48-59
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• 2019

최근 들어 장대교량의 건설에 현수교, 사장교 등과 같은 케이블이 주 요소인 교량 형식이 급증하고 있다. 이와 같은 구조물에서 케이블이 미치는 영향은 매우 크며, 구조해석을 위해서 케이블에 대한 연구 및 케이블의 모드 특성에 따른 구조계의 변화를 연구해 볼 필요가 있다. 특히, 케이블은 거더에 가해지는 하중효과를 주탑으로 전달하는 캠버조절과 직결되고 전체 구조에서 중요한 부재로서, 가설시 가해지는 초기 장력과 시간이 경과후 장력을 비교하므로서 교량의 노후상태와 이상유무를 파악하는 데 기여하고자 본 연구를 수행하였다. 따라서 본 연구에서는 충격해머를 통한 모드 해석으로부터 케이블의 질량과 케이블의 길이변화에 따른 모드의 특성을 파악할 수 있었고, 공용중에 있는 케이블에서는 위상각의 변화가 비례관계임을 확인하였다. 또한 저차모드에서 지배모드가 결정되는 특성을 MAC분석을 통하여 알 수 있었다.

• 한국방사선학회논문지
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• 제15권4호
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• pp.565-573
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• 2021

인체 내 전자기장의 공간 분포는 고 유전율 재료를 사용하여 비교적 간단하게 조정할 수 있다. 이 방법은 다른 방법에 비해 보완적인 성격이 강하지만 특정 응용 분야에서 강력한 유전체 시밍용 도구로 활용될 수 있다. 기하학적으로 자유로운 형상으로 제조 가능하며 시스템의 어떠한 변경 없이 목적에 따라 제작된 패드를 적용할 수 있다. 특히 초 고자장(ultrahigh magnetic field UHF) MRI에서 높은 작동 주파수로 인해 낮은 감도 (low sensitivity)를 갖는 송신 (B1+) 및 수신 (B1-) 필드의 강도를 높이는 데 사용되는 임상목적의 고 유전율 패드는 잠재적 가치가 상당히 클 뿐만 아니라 그 효과가 클 것으로 예상되는 UHF MRI에 적용된 연구가 적기 때문에 이 연구에서는 티탄산 칼슘의 현탁액으로 제조된 고 유전율 패드를 실험실에서 직접 개발하였으며 UHF 7T 자기공명영상 MRI의 다양한 프로토콜에서 임상적으로 유용한 영상의 신호증가를 확인하였다.

• 동의생리병리학회지
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• 제36권2호
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• pp.66-72
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• 2022

Flos Lonicerae Japonicae (the flower buds of Lonicera japonica Thunberg) has been used as an antibacterial and antiviral drug in Korean Medicine. The aim of this study is to evaluate the effect of Flos Lonicerae Japonicae water extract (FL) on the production of cytokines in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). After 24 h treatment, the production of various cytokines from RAW 264.7 was measured with multiplex cytokine assay using Bio-Plex 200 suspension array system. FL at concentrations of 50, 100, and 200 ㎍/mL significantly inhibited productions of tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1β, and MIP-2 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly inhibited productions of leukemia inhibitory factor, LIX (CXCL5), and RANTES in LPS-stimulated RAW 264.7 cells; FL at concentrations of 200 ㎍/mL significantly inhibited productions of granulocyte-macrophage colony-stimulating factor and macrophage colony-stimulating factor in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50 and 100 ㎍/mL significantly increased productions of interleukin (IL)-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50, 100, and 200 ㎍/mL significantly increased productions of IL-6 and interferon gamma-induced protein-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly increased productions of monocyte chemoattractant protein-1 in LPS-stimulated RAW 264.7 cells. Taken together, these data mean that FL might modulate productions of cytokines, chemokines, and growth factor in LPS-stimulated macrophages. Further study needs to verify the exact mechanism for modulatory activities of FL with macrophages.