• KSBB Journal
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• v.7 no.2
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• pp.96-101
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• 1992

A low serum medium(LSM) suitable for the growth of a self-constructed hybridoma cell line, KA112, was established by selecting ingredients to replace serum. Insulin and sodium pyruvate was important for the growth of cell line KA112. Various basal media were tested and DMEM gave the most favorable result. Low serum medium (LSM) developed in this work showed cell line stability in the culture for more than 6 months and exhibited cell growth equivalent to that carried out in medium supplemented with 7% FBS. LSM was found to be applicable to the suspension culture of KA112. The reduction of serum level down to 1%(V/V) FBS in LSM resulted in a substantial saving in the cost of media preparation for large scale culture.

• Journal of Environmental Science International
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• v.11 no.6
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• pp.597-603
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• 2002

Photocatalytic degradation of phenol was carried out with UV-illuminated TiO$_2$-SiO$_2$ in aqueous suspension. TiO$_2$-SiO$_2$ catalysts were prepared by sol-gel method from the titanium isopropoxide and tetraethylorthosilicate at different Ti/Si ratio and some commercial TiO$_2$ catalysts were used as purchased. All catalysts were characterized by X-ray Diffraction(XRD) and BET surface area analyzer. The effect of reaction conditions, such as initial concentration of phenol, reaction temperature and catalyst weight on the photocatalytic activity was studied. In addition, TiO$_2$-SiO$_2$(49: 1) prepared by sol-gel method showed higher activity than commercial TiO$_2$catalysts on the photocatalytic degradation of phenol. The addition of SiO$_2$ into TiO$_2$hepled to increase the thermal stability of titania which suppressed the formation of anatase into rutile. The photocatalytic degradation of phenol showed pseudo-1st order reaction and the degradation rate increases with decreasing initial phenol concentration.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.22 no.1
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• pp.112-118
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• 2013

Formula race car is generally recognized as a vehicle which is optimally designed for on-road race track with the regulations of race host bodies. Especially, the uprights of suspension system decisively have effects on the performance of cornering and stability of race car's driving performance, which are very important factors in the design of race car. This paper is a study of optimal upright design of F1800 grade formula race car which are normally used in professional race circuit in Korea. To design optimally the front upright of F1800 formula race car, Taguchi's orthogonal array, which is known for more useful method than full factorial design experimental method in cost and time, is used with CAE method such as FEM analysis. And the result of this paper shows that Taguchi's orthogonal array employed for this optimal design is very useful for designing the front upright of race car by minimizing its weight as well as keeping its safety factor as enough as designer wants in the view of quality, cost and delivery at the early design step.

• Korean journal of food and cookery science
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• v.11 no.2
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• pp.113-118
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• 1995

A preferable mixing ratio of a six-vegetable juice was suggested in this study. The vegetables used for preparation of mixed vegetable juice were carrot(Ct), cabbage(Cg), pear(Pr), cucumber(Cr), celery (Cy) and dongchimi(Di). The characteristics of pH, titratible acidity, reducing sugar, turbidity, solids, color and acceptability were compared to determine the mixing ratio. The vegetables showed a wide ranHe of pH of 3.70-6.01, acidity of 28.92 uv~74.40 nd and reducing sugar of 1.20ft~ 12.69fo. Celery juice showed the higest suspension stability and "b" value and the lowest values in Hunter "L" and "a" values among the 6 vegetable juice. The preferable mixing ratio of two-vegetable juice selected were Ct-Di(1 : 4), Cg-Pr(1 : 3) and Cr-Cy(3 : 1). From the various ratio of the three of biary mixtures of Cg-Pr(1 : 3): Ct-Di(1 : 4): Cr-Sy(3 : 1), two ratio of 5.0 : 2.5 2.5(V-6A) and 6.0 : 2.0 : 2.0(V-6B) were suggested as the most prefered six-vegetable juice. Pear, dongchimi and cucumber were found to be influential on the preference. The pH and titratible acidity of the two juices with different ratios were in the range of 4.92~4.98 and 36.g∼37.4 ml, respectively.

• The Journal of Advanced Prosthodontics
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• v.3 no.1
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• pp.20-24
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• 2011

PURPOSE. The aim of this study was to identify in vitro antimicrobial activity of the tissue conditioner containing silver nanoparticles on microbial strains, Staphylococcus aureus, Streptococcus mutans and Candida albicans. MATERIALS AND METHODS. Experimental disc samples ($20.0{\times}3.0$ mm) of tissue conditioner (GC Soft-Liner, GC cooperation, Tokyo, Japan) containing 0.1 - 3.0% silver nanoparticles (0%: control) were fabricated. Samples were placed on separate culture plate dish and microbial suspensions (100 ${\mu}L$) of tested strains were inoculated then incubated at $37^{\circ}C$. Microbial growth was verified at 24 hrs and 72 hrs and the antimicrobial effects of samples were evaluated as a percentage of viable cells in withdrawn suspension (100 ${\mu}L$). Data were recorded as the mean of three colony forming unit (CFU) numerations and the borderline of the antimicrobial effect was determined at 0.1% viable cells. RESULTS. A 0.1% silver nanoparticles combined to tissue conditioner displayed minimal bactericidal effect against Staphylococcus aureus and Streptococcus mutans strains, a 0.5% for fungal strain. Control group did not show any microbial inhibitory effect and there were no statistical difference between 24 hrs and extended 72 hrs incubation time (P > .05). CONCLUSION. Within the limitation of this in vitro study, the results suggest that the tissue conditioner containing silver nanoparticles could be an antimicrobial dental material in denture plaque control. Further mechanical stability and toxicity studies are still required.

• Journal of Electrical Engineering and Technology
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• v.8 no.6
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• pp.1451-1456
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• 2013

This paper presents the improved zero power levitation control algorithm for a quadruple hybrid EMS (Electromagnetic Suspension) system. Quadruple hybrid EMS system is a united form of four hybrid EMS systems one on each corner coupled with a metal plate. Technical issue in controlling a quadruple hybrid EMS system is the permanent magnet's equilibrium point deviation caused by design tolerance which eventually leads to a limited zero power levitation control that only satisfies the zero power levitation in one or two hybrid EMS system among the four hybrid EMS system. In order to satisfy a complete zero power levitation control of the quadruple hybrid EMS system, the proposed method presented in this paper adds a compensating algorithm which adjusts the gap reference of each individual axe. Later, this paper proves the stability and effectiveness of the proposed control algorithm via experiment and disturbance test.

• International Journal of Automotive Technology
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• v.5 no.2
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• pp.77-88
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• 2004

Narrow commuter vehicles can address many congestion, parking and pollution issues associated with urban transportation. In making narrow vehicles safe, comfortable and acceptable to the public, active tilt control systems are likely to playa crucial role. This paper focuses on the development of an active direct tilt control system for a narrow vehicle that utilizes an actuator in the vehicle suspension. A simple PD controller can stabilize the tilt dynamics of the vehicle to any desired tilt angle. However, the challenges in the tilt control system design arise in determining the desired lean angle in real-time and in minimizing tilt actuator torque requirements. Minimizing torque requirements requires the tilting and turning of the vehicle to be synchronized as closely as possible. This paper explores two different control design approaches to meet these challenges. A Receding Horizon Controller (RHC) is first developed so as to systematically incorporate preview on road curvature and synchronize tilting with driver initiated turning. Second, a nonlinear control system that utilizes feedback linearization is developed and found to be effective in reducing torque. A close analysis of the complex feedback linearization controller provides insight into which terms are important for reducing actuator effort. This is used to reduce controller complexity and obtain a simple nonlinear controller that provides good performance.

• Proceedings of the KSR Conference
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• 2007.05a
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• pp.99-108
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• 2007

The bi-modal tram guided by the magnetic guidance system has two car-bodies and three axles. Each axle of the vehicle has an independent suspension to lower the floor of the car and improve ride quality. The turning radius of the vehicle may increase as a consequence of the long wheel base. Therefore, the vehicle is equipped with the All-Wheel-Steering(AWS) system for safe driving on a curved road. Front and rear axles should be steered in opposite directions, which means a negative mode, to minimize the turning radius. On the other hand, they also should be steered in the same direction, which means a positive mode, for the stopping mode. Moreover, only the front axle is steered for stability of the vehicle upon high-speed driving. In summary, steering angles and directions of the each axle should be changed according to the driving environment and steering mode. This paper proposes an appropriate AWS control algorithm for stable driving of the bi-modal tram. Furthermore, a multi-body model of the vehicle is simulated to verify the suitability of the algorithm. This model can also analyze the different dynamic characteristics between 2WS and AWS.

• Journal of the Korean Applied Science and Technology
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• v.14 no.1
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• pp.109-115
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• 1997

Fluorescent anionic oligo surfactants were synthesized by the condensing products of long chain alkylvinylether-maleic anhydride cooligomers and resorcinol including dye structures. Their various surface activities and dispersing action were studied on the aqueous solution. These oligo surfactants exhibited a remarkable surface tension lowering property, lower foaming and a large dispersing action for the particles of ${\alpha}-copper$ phthalocyanine blue. Further it was ascertained that the binding of oligo surfactant onto the pigment surface caused the deviation towards lower wavelengths at the maximum fluorescent intensity as compared with aqueous oligo surfactant solutions, These surface active properties of the oligo surfactants may be attributed to rigid and hydrophobic structure of dye groups, besides surface-active groups of alkylether groups and carboxylic group of the anionic oligo surfactants.

• Transactions of the Korean Society of Automotive Engineers
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• v.20 no.2
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• pp.47-55
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• 2012

This work deals with dynamic analysis of a monorail system with magnetic caterpillar where magnets are embedded inside each articulated element of the caterpillar, augmenting traction force of main rubber wheels to climb up slope up to 15 degree grade. Considerations are first given to determine stiffness of the primary and secondary suspension springs in order for the natural frequencies of car body and bogie associated with vertical, pitch, roll and yaw motion to be within generally accepted range of 1-2 Hz. Equations for calculating magnetic force needed to climb up given slope are derived, and a magnetic caterpillar system for 1/6 scale monorail is designed based on the derivation. To assess the hill climbing ability and cornering stability, and make sure smooth operation of the side and vertical guiding wheels which is critical for safety, a multibody model that takes into account of every component level design characteristics of car, bogie, and caterpillar is set up. Through hill climbing simulation and comparison with measurement of the limit slope, the validity of the analysis and design of the magnetic caterpillar system are demonstrated. Also by studying the curving behavior, maximum curving speed without rollover, functioning of lateral motion constraint system, the effects of geometry of guiding rails are studied.