• Korean Journal of Environmental Biology
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• v.32 no.3
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• pp.159-167
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• 2014

In this study, I examined the water quality and phytoplankton community in aquaculture laver farm in the southwest part of Aphae-do, South Korea, based on the young leaf stage, middle leaf stage, and adult leaf stage of laver thallus from October, 2013 to January, 2014. It was observed that the Aphae laver farm, as located in shallow waters, was found to have a serious resuspsension of the surface sediments due to physical disturbance caused by winds and tidal mixing. Such a resuspension of surface sediments coupled with nutrients supply obstructs light penetration into the sea for its huge amount of total suspended matters. As a result for this reason, it was viewed toimpedthe growth of phytoplankton was impeded as it also competes with laver to absorb the same kinds of nutrients as laver does during the laver growth period in winter. Such elements of the marine environment in Aphae laver farm are in contrast with the environment of Japan, where nutrients including dissolved inorganic nitrogen, in particular, are insufficient to cause the recent laver bad harvest, discoloration and quality degradation while large diatoms, with their higher nutrients absorption efficiency than laver, generate winter red tide. In other words, an important factor to maintain the high laver production in the southern parts of West Sea of Korea was found to be the marine environment of its laver farms where large diatoms are prevented from growing due to nutrients supply and dense seston weights from resuspended matters by physical disturbances.

• Journal of Wetlands Research
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• v.20 no.3
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• pp.235-242
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• 2018

The function of vertical subsurface flow wetlands can potentially be reduced with time due to clogging and are often assumed to be occurring when ponding and overflow is observed during rainfall. To investigate their clogging potential, three pilot-scale vertical subsurface flow (VSF) wetland systems were constructed employing woodchip, pumice, and volcanic gravel as main media. The systems received stormwater runoff from a highway bridge for seven months, after which the media were taken out and divided into layers to determine the amount and characteristics of the accumulated clogging matters. Findings revealed that the main clogging mechanism was the deposition of suspended solids. This is followed by the growth of biofilm in the media which is more evident in the wetland employing woodchip. Up to more than 30% of the clogging matter were found in the upper 20 cm of the media suggesting that this layer will need replacement once clogging occurs. Moreover, no signs of clogging were observed in all the wetlands during the operation period even though an estimation of at least 2 months without clogging was calculated. This was attributed to the intermittent loading mode of operation that gave way for the decomposition of organic matters during the resting period and potentially restored the pore volume.

• Korean Journal of Ecology and Environment
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• v.42 no.2
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• pp.161-171
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• 2009

A 'continuous removal of organic matters (CROM) system' using a native freshwater bivalve in Korea Anodonta woordiana, was developed to determine its potential of controlling various sestons in eutrophic lake system, and to evaluate its effect on water quality improvement under consideration of sediment addition as habitat. We designed CROM experiments with four treatments: no mussels and no sediment (W, negative control), no mussels and sediment (WS, positive control), mussels and no sediment (WM), and mussels and sediment (WMS). The experiments were performed at the condition of 18${\sim}$25 L $h^{-1}$ of inflow, mussel density of 486.1 indiv. $m^{-2}$, and temperatures between 15 and $22^{\circ}C$ for 13 consecutive days. Physicochemical and biological parameters were measured at daily (10:00 am) intervals after the mussel addition. Results indicated that mussel stockings without addition of sediment effectively removed sestons (suspended solids and chlorophyll-a) at nearly same level over 80 percentage of the control during the study, while there were no differences in removal activities of sestons between with and without sediment (P>0.5). Therefore, it clearly suggests that CROM system using A. woordiana has a strong potential to control the seston in surface water of eutrophic lake.

• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.89-94
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• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• Journal of Korean Society on Water Environment
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• v.29 no.4
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• pp.540-550
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• 2013

Temporal increase of SS induces concentrations in various forms of organic matter including BOD, COD, TOC. Consequently, it causes hard to identify sources of water pollution during or after precipitation. The objective of this study is to investigate variations of DOC concentration caused by increase of flow and changes of external factors in river by comparing to SS concentration. In results, monitoring sites (e.g., Banbyeonchen) consisting of hard riverbed showed high correlation between SS and organic matters, except BOD. On the contrary, other sites (e.g., Naesungcheon) where riverbed consists of sand were found in a wide range of annual fluctuation in SS level, whereas these sites showed a narrow range in annual DOC fluctuation. In Gumhogang and Namgang, a lower correlation between SS and other factors was found most likely because of high concentration in organic matter. However, lower annual fluctuation values of DOC were observed in comparison to those of COD and TOC. Similar results were also confirmed in main river sites, Sangju and Mulgeum. In conclusion, DOC concentration is better indicator for monitoring organic matter which cannot be provided by BOD, COD, TOC in the Nakdong river basin.

• Journal of the Korean Institute of Gas
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• v.1 no.1
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• pp.101-105
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• 1997

When the water curtain system is employed to keep the liquefied gas in the underground storage cavern, clogging is observed in borehole. Since this phenomenon causes serious difficulties in managing LPG storage cavern, it needs to detect the degree of clogging accurately under various circumstances. Thus, in this study the active factors of clogging, that is, groundwater head, injection water flowrate, and seepage water flowrate, were investigated experimentally using a physical model. Experimental results show that groundwater head around storage cavern increases as cavern Pressure increases, while it decreases as clogging becomes severe. The pressure in storage cavern is required to reduce up to atmospheric pressure in order to detect and identify the degree of clogging more accurately. The decrease of uroundwater head due to clogging slows down as the pressure in borehole increases. As amounts of suspended matters in injected water increase, both injection water flowrate and seepage water flowrate decrease linearly with time, and the flowrate of injection water drops rapidly compared with seepage water flowrate.

• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.5
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• pp.57-65
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• 2007

The objective of this study was to evaluate chemical water quality by hourly monitoring(25hr) of Saemangeum esturary. For this study, we selected 2 sites like a Mangyong Bridge(St. 6) and Dongjin Bridge(St. 7). Inflow of salt water was not detected during low tide(maximum 553, 508cm) of all stations, while the salinity rises were detected in spring tide(750cm). When 602m of maximum tide was reached, salinity concentration was increased at St. 7, while there was no change in St. 6. Therefore, We know that salinity variation is greatly influenced by tide height at survey site. Also, significant variance of salinity(p<0.05) was found between St. 6 and St. 7 because dike construction made the flood tide move into the Dongjin river. Total suspened solids(TSS) concentration was increased because of the river runoff at St. 6, and also the turbulance and resuspension according to salt intrusion at St. 7. During the high tide, the water discharge from the sea seemed to dilute the nutrient but to elevate TSS concentration in St. 7. Silicate and nitrate concentrations in the studied site were decreased by the mixing of sea water, whereas the evident trend of phosphate concentration was not found. This result can be explained by the phosphorus condition. Phosphorus exists inactive when it is affected by hydrated iron and adsorbed onto suspended matters. Compared to the environmental conditions of the St. 6 and St. 7, physical factors such as temperature, dissolved oxygen and TSS have statistically no significant difference(p<0.001), but nutrient concentrations were higher at St. 6 than St. 7. It could be suggested from these results that it is important to control the discharge of fresh water by sewage treatment plants located in St. 6 for water quality management.

• Journal of Korean Society on Water Environment
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• v.32 no.1
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• pp.98-107
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• 2016

This study examined the effects of environmental conditions and the presence of refractory organic matter on oxidation rates of total organic carbon (TOC) measurements based on high temperature combustion and ultraviolet-sulfate methods. Spectroscopic indices for prediction of oxidation rates were also explored using the UV spectra and fluorescence excitation-emission matrix (EEM) of humic acids. Furthermore, optimum TOC instrument conditions were suggested by comparing oxidation rates of a standard TOC material under various conditions. Environmental conditions included salts, reduced ions, and suspended solids. Salts had the greatest influence on oxidation rates in the UV-sulfate method. However, no effect was detected in the high temperature combustion method. The UV-sulfate method showed lower humic substance oxidation rates, refractory natural organic matter, compared to the other methods. TOC oxidation rates for the UV-sulfate method were negatively correlated with higher specific-UV absorbance, humification index, and humic-like EEM peak intensities, suggesting that these spectroscopic indices could be used to predict TOC oxidation rates. TOC signals from instruments using the UV-sulfate method increased with increasing chamber temperature and increasing UV exposure durations. Signals were more sensitive to the former condition, suggesting that chamber temperature is important for improving the TOC oxidation rates of refractory organic matter.

• Journal of Korean Society on Water Environment
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• v.25 no.1
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• pp.48-57
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• 2009

A two-dimensional (2D), laterally-averaged hydrodynamic and water quality model, CE-QUAL-W2 was applied to evaluate the performance on simulating the effect of flushing from Daecheong Reservoir on the downstream water quality variations during the flushing events held on November, 2003 and March, 2008. The hydraulic and water quality simulation results were compared with field measurement data, as well as a one-dimensional (1D), unsteady model (KORIV1) that revealed limited capability in the previous study due to missing the resuspension process of river bottom sediments. The results showed that although the 2D model made satisfactory performance in reproducing the temporal variations of dissolved matters including phosphate, ammonia and nitrate, it revealed poor performance in simulating the increase of biological oxygen demand and suspended sediment (SS) concentrations during the passage of the flushing flow. The reason of the error was that the resuspension process of the 2D model is only the function of shear stress induced by wind. In reality, however, as shown by significant correlation between bottom shear stress ($\tau$) and observed SS concentration, the resuspension process can be significantly influenced by current velocity in the riverine system, especially during flushing event. The results indicate that the resuspension of river bottom materials should be incorporated into the water quality modeling processes if $\tau$ is greater than a critical shear stress (${\tau}_c$) for better simulation of flushing effect.

• Journal of Korean Society on Water Environment
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• v.25 no.5
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• pp.768-777
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• 2009

The characteristics of stormwater runoff was examined on distinct types of agricultural and industrial area in Lake Sihwa watershed. During rainfall event, the peak concentrations of SS, $COD_{Mn}$, and TP were observed after 6~11 hours of rainfall in agricultural areas. Whereas, the peak concentrations occurred within the first one hour after rainfall and then the highest concentration of NPS pollutants sharply decreased, showing strong first flush effect in industrial areas. The strong first flush effect of suspended solid was apparent in agricultural areas, while those of organic matters and nutrients were clear in industrial areas. The cumulative load curves for NPS pollutants showed above the $45^{\circ}$ straight line, indicating that first flush effect occurred in industrial areas. The mean SS EMC values of agricultural areas ranged from 60~598 mg/L (Avg. 285 mg/L), it was higher value when compare to other areas. While the mean $COD_{Mn}$, TN, and TP EMCs values of industrial areas were shown the highest values as 67.7 mg/L, 12.1 mg/L and 2.1 mg/L respectively.