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Correlation between operation factors and nitritation using anaerobic digester supernatant at ordinary temperature (상온 조건에서 혐기 소화 상징액을 이용한 아질산화 반응과 운전 인자의 상관성 분석)

  • Im, Jiyeol;Gil, Kyungik
    • Journal of Wetlands Research
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    • v.18 no.3
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    • pp.244-249
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    • 2016
  • Anaerobic digester supernatant including high concentrations of nitrogen is recycled to water treatment line and make pollutant load increase in municipal wastewater treatment plant(MWTP). To treat nitrogen in anaerobic digester supernatant is suggested the method of MWTP retrofit. In this study, the lab scale reactor was operated about 200 days using supernatant of anaerobic digester. The results could draw operation condition that ammonium nitrogen removal efficiency more than 90% and nitrite conversion efficiency over 70%. Correlation between operation efficiency and operation factors was analyzed based on the operation results. Ammonium nitrogen remove efficiency and nitrite conversion efficiency were related to solid retention time (SRT), ammonium nitrogen load and ammonium nitrogen loading per unit mixed liquer suspended solid (MLSS). Results of this study can be used effective data on nitritation of supernatant of anaerobic digester, and be expected to increase availability of nitritation.

Fatigue performance and life prediction methods research on steel tube-welded hollow spherical joint

  • Guo, Qi;Xing, Ying;Lei, Honggang;Jiao, Jingfeng;Chen, Qingwei
    • Steel and Composite Structures
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    • v.36 no.1
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    • pp.75-86
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    • 2020
  • The grid structures with welded hollow spherical joint (WHSJ) have gained increasing popularity for use in industrial buildings with suspended cranes, and usually welded with steel tube (ST). The fatigue performance of steel tube-welded hollow spherical joint (ST-WHSJ) is however not yet well characterized, and there is little research on fatigue life prediction methods of ST-WHSJ. In this study, based on previous fatigue tests, three series of specimen fatigue data with different design parameters and stress ratios were compared, and two fatigue failure modes were revealed: failure at the weld toe of the ST and the WHSJ respectively. Then, S-N curves of nominal stress were uniformed. Furthermore, a finite element model (FEM) was validated by static test, and was introduced to assess fatigue behavior with the hot spot stress method (HSSM) and the effective notch stress method (ENSM). Both methods could provide conservative predictions, and these two methods had similar results. However, ENSM, especially when using von Mises stress, had a better fit for the series with a non- positive stress ratio. After including the welding residual stress and mean stress, analyses with the local stress method (LSM) and the critical distance method (CDM, including point method and line method) were carried out. It could be seen that the point method of CDM led to more accurate predictions than LSM, and was recommended for series with positive stress ratios.

Regeneration of Bovine Mammary Gland in Immunodeficient Mice by Transplantation of Bovine Mammary Epithelial Cells Mixed with Matrigel

  • Park, Hyun Jung;Lee, Won Young;Jeong, Ha Yeon;Song, Hyuk
    • International Journal of Stem Cells
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    • v.9 no.2
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    • pp.186-191
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    • 2016
  • Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

Data Acquisition and Statistical Processing of Insulation Resistance for High-Power Cables in Operation (운전 중 고전력 케이블의 절연저항 데이터의 취득과 통계적 처리 방법)

  • Park, Sung-Hee;Um, Kee-Hong
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.6
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    • pp.181-186
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    • 2018
  • With progress in industrialization, facilities for generating, delivering, and receiving high levels of electric power are in great demand. The scale of electric power equipment is increasing in both size and complexity. This has contributed to the development of our modern, high-tech and information-based society. However, if the generation of electric power is suspended due to unexpected accidents at power facilities or power stations, a range of equipment the operations of which are dependent on electric power can be damaged, causing substantial socioeconomic losses in an industrial society. A great deal of time and money would be expended to repair damaged facilities at a power station, causing enormous economic loss. In order to detect the deterioration processes of power cables, and to prevent the destruction of power cables, the operation status of power cables should be monitored on a regular basis. We studied the method in order to improve accuracy and reliability for diagnosising the junction where accident occurs frequently. We present the method of data acquisition and statistical processing.

An Experimental Study of Sediment Transport Patterns behind Offshore Structure (외해 구조물 배후의 표사이동에 관한 실험적 연구)

  • Shin Seung-Ho;Hong Keyyong
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.4
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    • pp.207-215
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    • 2004
  • Recently, securing a vast land in the land region becomes more difficult and efforts to seek its alternation in the sea area have been increased. As a consequence, the coastal region has been faced to extensive beach erosion problems. In planning offshore structures such as artificial islands, it is necessary to forecast the influence of the structure construction exerting on the beach erosion of the adjacent coast. In the present study, the sediment movement pattern behind offshore structure was examined through a series of three dimensional movable bed experiments, so as to develop the numerical model which forecasts morphological change including beach erosions. The experimental results reveal that the sediment movement patterns of the beach line side and the depth region are separated at a certain boundary line. In details, at the beach side including swash zone the sediment movement becomes dominant, which is governed by a relation between depth contours and incident wave directions, while at the depth region the bed load and suspended load due to the orbit motion of waves are carried by nearshore currents, and both movements are clearly separated at a specified boundary that is related to partial standing wave from the beach. It is expected that these results can be effectively used for verification of a numerical model on morphological change of the coast.

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Distribution of Surface Sediments and Sedimentation Rates on the Tidal Flat of Muan Bay, Southwestern Coast, Korea (서해남부 무안만의 표층퇴적물의 분포와 조간대 퇴적률)

  • Ryu, Sang-Ock;Kim, Joo-Young;Chang, Jin-Ho
    • Journal of the Korean earth science society
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    • v.22 no.1
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    • pp.30-39
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    • 2001
  • To consider the distribution of the surface sediments and sedimentary processes in tidal flat after the construction of sea-walls in the semiclosed Muan Bay, the southwest coast of Korea, studies on the sediments and sedimentation rates along two transect lines from February 1999 to March 2000 were carried out. The surface sediments in the bay are classified into 5 facies on the basis of textural parameters. The gravelly sediments including the cobbles and pebbles deposited in the bay-mouth are relic sediments which are formed in nearshore and fluvial environments at the low stand of sea level. In addition, it is considered that the muddy sediments widely distributed on the tidal flat might be originated from suspended matter transported from several rivers and/or offshore. Although the sedimentation rates on the tidal flat vary with season, their estimation rates are -8.9 mm/yr on Line-GR and -48.9 mm/yr on Line-YH, respectively. The erosion-dominated processes in the tidal flat would be caused by strengthened ebb tides due to the decrease of tidal choking at Mokpogu and the migration of tidal currents at the bay-mouth. The hydrodynamical changes mentioned above are interpreted to be occurred after/before the construction of sea-dike/sea-walls in coastal zone of Mokpo.

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Modelling Algae Transport in Coastal Areas with Marine Afforestation (바다숲 조성해역의 해조류 포자 확산모델링)

  • Cho, Jae-Kweon;Lim, Young-Soo;Hong, Do-Ung;Kim, Jong-Kyu
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.15 no.1
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    • pp.1-8
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    • 2012
  • To arrange effectively artificial reefs for marine afforestation, tidal currents were analyzed by numerical experiments, and particle tracking based upon tidal currents were carried out to clarify the path of algae spore. The experiments were carried out by EFDC (Environmental Fluid Dynamics Code), and water column was vertically divided 10 layers. Tidal current patterns showed to be affected by main current at outside of study area, and circle currents of two were observed from analysis of residual currents. Particle tracking were experimented for 15 days at 2 installation places in which artificial reefs for marine afforestation would be deployed. According to the results of particle tracking experiment, particle movement at St.1 showed belt type along coastal line, and St.2 showed ellipse type at 300~500 m distant from coastal line. It suggest that artificial reefs for marine afforestation should be installed belt zone at station of St.1 and ellipse zone at St.2. Modelling algae transport was also tested to account for local dispersion of algae spore due to the suspended materials.

A dual-frequency and dual-polarization antenna with enhanced isolation between two ports using mushroom-like EBGs (버섯모양 EBG를 이용하여 두 포트 사이의 고립도를 향상시킨 이중대역 이중편파 안테나)

  • Lee, Dong-Hyun;Kim, Jae-Hee;Jang, Jong-Hoon;Park, Wee-Sang
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.5
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    • pp.70-75
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    • 2007
  • A dual-frequency dual-polarization (DFDP) antenna with high isolation between two ports by embedding $2{\times}1$ mushroom-like electromagnetic bandgap (EBG) cells is proposed. The equivalent circuit of a suspended microstrip line over $2{\times}1$ EBG cells is introduced. The numerical analysis from the equivalent circuit and measured results show that the microstrip line with embedded EBG cells has a distinctive and sharp rejection band and provides near 0 dB insertion loss outside the rejection band. By embedding the EBG cells under feedlines of a conventional DFDP antenna, the isolation between two ports of the antenna is enhanced more than 20 dB, as compared to that of a conventional DFDP antenna. The proposed DFDP antenna is fabricated and measured. The simulated and measured results show a good agreement. The measured polarization purity and gain of the antenna are 25 dB and 5.77 dBi at lower band, and 35 dB and 7.13 dBi at higher band, respectively.

Cytokine Induction of Intercellular Adhesion Molecule-1(ICAM-1) Expression on Human Glioblastoma Cell Line, U-251 MG, U-373 MG (교모세포종 U-251MG, U-373MG세포주의 Cytokines처리에 의한 세포내 ICAM-1 발현)

  • Lee, Jong-Won;Kwon, Jung-Taek;Min, Byung-Kook;Park, Seung-Won;Kim, Young-Baeg;Hwang, Sung-Nam;Suk, Jong-Sik;Choi, Duck-Young
    • Journal of Korean Neurosurgical Society
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    • v.29 no.4
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    • pp.477-484
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    • 2000
  • Objective : Despite advances in the understanding of tumor biology and the tumor immunology, there has been no effective treatment. The Intercellular adhesion molecule-1(ICAM-1) has been shown to be important in interaction involving cells of the immune system and to be upregulated in a number of cell culture systems by cytokines, including immune interferon($IFN-{\gamma}$) and tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$). ICAM-1 has been identified as one of the ligands for lymphocyte function-associated antigen-1(LFA-1). The effectiveness of various cytokines to ICAM-1 induction on cultured human glioblastoma cell lines and potential efficacy of immunotherapy were studied. Method : Human glioblastoma cell lines, U-251 MG, U-373 MG were trypsinized and suspended at $1{\times}10^5cells/ml$ and grown on 8 well chamber slide, the cells were incubated in 0.3ml medium alone or medium containing $IFN-{\gamma}$(1000U/ml) or $TNF-{\alpha}$(250U/ml) or $IFN-{\gamma}$ plus $TNF-{\alpha}$ for 6, 12, 24, 48 and 72 hours. The coverslip were then removed and stained with a 1/30 dilution of anti-ICAM-1 antibody. Result : Surface antigen expression of ICAM-1 was increased by incubating glioblastoma cell lines with $IFN-{\gamma}$ and $TNF-{\alpha}$. Combined effect of $IFN-{\gamma}$ and $TNF-{\alpha}$ has induced more ICAM-1 expression on glioblastoma cell lines. Upregulation of ICAM-1 expression in an established glioblastoma cell line was of greater magnitude and more rapid following incubation with $IFN-{\gamma}$ plus $TNF-{\alpha}$. Surface antigen expression of ICAM-1 was increased for up to 48 hours after cytokine treatment on both cell lines(p<0.05). There was no difference on both cell lines(p>0.05). Conclusion : The results of the present study indicate that ICAM-1 expression in glioblastoma cell lines, U-251 MG and U-373 MG, are induced and enhanced after treatment with $IFN-{\gamma}$ and $TNF-{\alpha}$. Combined effect of $IFN-{\alpha}$ and $TNF-{\gamma}$ is stronger and more rapid than $IFN-{\gamma}$ or $TNF-{\alpha}$ alone.

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A Study on the Traditional Costumes and Tattoo of the Maori (마오리族 傳統 服飾과 文身 考察)

  • 황춘섭;정현주
    • The Research Journal of the Costume Culture
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    • v.3 no.2
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    • pp.241-260
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    • 1995
  • The Maori's traditional clothing materials, basic forms of dress, and the pattern and technique of tatoo were examined in the present study in order to deepen the appreciation of the cultural heritage of the Maori. The research method employed was the analysis of written materials. And a fild-trip was also made for the study. The study was limitted to the traditional culture of body adornment of the Maori including the clothing which is preserved and practicing by them at the present day, and the origin and the process of the historical development of those are not included in the scope of the present study. Followings are the results of the study: (1) By far the most widely used fiber for Maori clothing is abtained from what is commonly called New Zealand Flax. The fiber of kiekie(Freycinetia baueriana) and cabbage trees(Cordyline spp.) may also be used. The strong, long-lasting fiber of toi(cordyline indivisa) is used for a prestige warrior's cloak. Flat strips of ti kauka(Cordyline australi) are also used as thatch on rain cloaks. (2) Regardless of technique used, Maori weaving is always worked horizontally from left to right. Traditionally the work was suspended between two upright turuturu or weaving sticks. As the work progressed a second pair of uprights was used to keep the work off the ground. These uprights were moved forward as required. Because the weaver sat on the ground, the working edge was kept at a height that was comfortable to reach. No weaving tools are used, the wefts(aho) being manipulated by the fingers. The two main Maori weaving techniques are whatu aho patahi(single-pair twining) and whatu aho rua(double-pair twining). (3) The Maori wore two basic garments - a waist met and a cloak. The cloth of commoners were of plain manufacture, while those of people of rank were superior, sometimes being decorated with feather or dyed tags and decorated borders. Children ran more-or-less naked until puberty, being dressed only for special events. Some working dress consisted of nothing more than belts with leaves thrust under them. Chiefs and commoners usually went barefoot, using rough sandals on journeys over rough country (4) The adornment of men and women of rank was an important matter of tribal concern as it was in chiefly persons that prestige of the group was centred, The durable items of Maori persons adornment were either worn or carried. Ornaments of various kinds were draped about the neck or suspended from pierced earlobes. Combs decorated the head. Personal decorations not only enhanced the appearance of men and women, but many had protective magical function. The most evident personal ornament was the hei-tiki made of jade or other material. Maori weapons were treasured by their owners. They served on bottle and were also personal regalia. A man of rank was not fully dressed without a weapon in hand. Also weapons were essential to effective oratory. (5) No man or woman of rank went without some tattoo adornment except in extremely rare instances when a person was too sacred to have any blood shed. The untattooed were marked as beeing commoners of no social standing. This indelible mark of rank was begun, with appropriate rite and ritual, at puberty. And tattoo marked the person as being of a marriageable age. Maori tattoo was unlike most traditional tattoo in that its main line were 'engraved' on the face with deep cuts made by miniature bone chisels. The fill-in areas were not tattooed with cuts but with the multiple pricks of small bone 'combs' that only lightly penetrated the skin surface. The instrument of tattoo consisted of small pots of pumice or wood into which was placed a wetted black pigment made from burnt kauri gum, burnt vegetable caterpillars or other sooty materials. A bird bone chisel or comb set at right angles on a short wooden handle was dipped into the gigment, that a rod or stick was used to tap head of this miniature adze, causing penetration of the skin surface. Black pigment lodged under the skin took on a bluish tinge. A full made facial tattoo consisted of major spirals with smaller spirals on each side of the nose and sweeping curved lines radiating out from between the brows over the forehead and from the nose to the chin. The major patterns were cut deep, while the secondary koru patterns were lightly pricked into the skin.

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