• 한국축산시설환경학회지
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• 제1권1호
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• pp.65-75
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• 1995

This experiment was carried out to the effects of population density and C/N ratios of feed on the growth, reproductive effciency and cast producation of the earthworm(Eisenia foetida). The population densities of 50, 100, 150, 200 and 250 individuals of the earthworm fed with different C/N ratios of 25, 35, 45 and 55 cow manures were studied in rearing box($6,400cm^3$), and at the fertility stage during a period of 60 days. The results were summarized as follows; The survial rate(SR), increasing rate(IR), reproductive efficiency(RE) and cast production of the earthworms showed highest values in C/N ratio of 25. These results may indicate that C/N ratio of 25 is a very favourable feed for the growth of the earthworms. The survial rate(SR) indicated significant positive correlation with reproductive efficiency(RE) in different C/N ratios of feeds. The survial rate(SR) showed highest values in population densities of $50{\sim}100$ worms/$6,400cm^3(64.0{\sim}128.0cm^3/worm$). On the contrary, increasing rate(IR) tended to decreased with the increased population densities. The survival rate(SR) indicated significant negative correlation with reproductive efficiency(RE) in different population densities of the earthworms. The cast production estimated were $31.6mg{\sim}67.4mg/day/worm$ grown in optimum population densities($50{\sim}100\;worms/6,400cm^3$). The earthworm casting are an excellent soil conditioning material or organic fertilizer sources with a high chemical composition and their physical properties.

• 한국양식학회지
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• 제11권2호
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• pp.125-132
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• 1998

1994년 7월 21일부터 1995년 10월 23일까지 논, 지수, 비닐하우스 야외사육지를 이용하여 동남참게를 사육한 결과, 1. 사육기간중 수온은 $1.5{\circ}C$~34.2${\circ}C$이었으나, 먹이를 정상적으로 먹은 기간의 수온은 15${\circ}C$~30${\circ}C$로 나타났으며, 이는 총 460일의 사육기간 중 281일(61.1%)에 해당되었다. 2. 증중량은 논(1,711.3 g), 지수(1,565.4 g), 비닐하우스(1,220.0 g)사육지의 순이었고, 생존율은 지수922.7%), 논(18.7%), 비닐하우스(18.3%)사육지의 순이었으며, 갑폭의 일간성장률은 논(0.61%), 지수(0.60%), 비닐하우스(0.59%)사육지의 순으로 논 사육지의 증중량 및 일간성장율이 타 사육지보다 다소 양호하였다. 3. 사육종료시 사육지별 갑폭의 출현모드를 보면 지수 및 비닐하우스 사육지의 모드는 3 cm군으로 나타난데 비하여 논 사육지는 모드가 4 cm군으로 나타났을 뿐 아니라, 증줄량과 일간성장율도 다소 높고 벼 경작도 병행할 수 있어 타 야외사육지보다 동남참게 양식에 적합한 사육지로 사료되었다. 4. 동남참게 내장의 일반성분을 분석한 결과 양식산이 자연산에 비하여 조지방 함량이 다소적게 나타난 것을 제외하고는 영양성분상의 큰 차이는 없었다.

• 대한치과의사협회지
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• 제49권5호
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• pp.279-285
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• 2011

Currently there is no dental ceramic material can be used in all dental situations need to be restored. However, in view of recent clinical reports, the most viable alternative is zirconia ceramic. Clinical success of dental zirconia restorations strongly depends on proper selection of materials, accurate laboratory procedure and final cementation, which can be achievable with the correct understanding of zirconia. As dental materials, zirconia ceramics have a very bright future, because they are being used increasingly in the anterior region as implant fixtures, as well as crown and bridge restorations and implant abutments. Many dental ceramics showing poor clinical performance have been gone from the dental market. However, in terms of outstanding mechanical properties and esthetic nature, new dental materials can replace zirconia ceramics will not be available in the foreseeable future.

• 한국잠사곤충학회지
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• 제27권2호
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• pp.1-6
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• 1985

상묘의 활착불량 원인을 구명하여 건전묘 생산을 위한 자료로 쓰기 위해 최근 3년동안 계속 발아가 불량한 묘목을 생산하였던 충북 옥천군 이원면에 소재한 논토양 상묘포지에 질소 과다 시용, 석회시용구, 붕사시용구, 잠시비료 1호시용구, 과도적엽구, 중간벌corn, 방임구 등 8처리와 생산자 상묘 등 모두 9개구에 대한 활착율 및 상묘 중의 화학 성분을 분석 조사한 결과 다음과 같았다. 1. 상묘의 활착율이 98% 이상을 보인구는 석회중화구와 잠시비료 1호구이었으며, 질소 과다 시용구는 59%로낮았으며, 과도 적엽구는 71%에 불과하였다. 2. 가지 표피와 뿌리 표피의 화학성분을 분석한 결과 활착율과 관계가 깊은 부위는 뿌리보다는 가지였으며, 성분 중 B, Cu, 그리고 Zn의 함량과 관계가 깊은 경향을 보였다. 3. 가지 표피 중의 질소함량은 질소과다 시용구에서 가장 높은 0.89%를 보였고, 통상 0.6∼0.7%이나, 과도적엽구와 중간벌채구에서는 0.5%정도로 낮았다. 4. 이상의 결과를 종합해 볼 때 활착불량 상묘의 발생원인을 묘포지의 토양 이화학성의 불량, 질소 과다 시용, 과도적엽 등이었으며, 활착율을 높히기 위해서는 이화학성이 좋은 포장을 묘포지로 선정해야 하며, 질소 과다 시용과 과도적엽 지양, 석회 또는 잠시비료 1호를 시용하여야 할 것으로 판단되었다.

• 한국수정란이식학회지
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• 제23권1호
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• pp.19-24
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• 2008

The purpose of this study was to compare the efficiency of slow freezing with that of vitrification method for the cryopreservation of human embryos. Human embryos were derived from in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and the mixed solution of propanedial (1.5, 1.0, 0.5M PROH) and sucrose (0.1M), ethylene glycol (7.5, 15%), dimethyl sulfoxide (7.5, 15% DMSO), sucrose (0.5, 1.0M) and SPS (Serum Protein Substitute) was used for a cryoprotectant for slow freezing and vitrification solution, respectively. Rates of recovery after thawing, morphological normality, post-thaw viability, arrest, morphological abnormality and preimplantation development were compared between two protocols. After freezing-thawing, recovery and survial rate of slow freezing was (88.6% and 73.4%), whereas vitrification was (99.2% and 96.2%) (p<0.05). The arrest rate of slow freezing was significantly lower compared with those of vitrification(8.7% vs 29.9%) (p<0.05). Preimplantation development to the 2-cell (83.8% vs 67.7%), 4-cell (69.0% vs 47.2%) and 8-cell (62.4% vs 37.8%) stages 24, 48 and 72 h after thawing, respectively, were higher in the slow freezing than the vitrification. After slow freezing and vitrification of human embryo at 2-8cell stage, the rate of recovery rate, survival rate and partial damage rate were 92.0% vs 100%, 80.4% vs 96.2% and 52.2% vs 19.0%, respectively. And partial damage rate was significantly lower than those of slow freezing method (p<0.05). These results demonstrate that a slow freezing using PROH is more efficient than a vitrification for cryopreserving the human zygotes, although the vitrification yielded better recovery, survival and partial damage of frozen-thawed 2-8 cell stage embryos than slow freezing method.

• 한국잠사곤충학회지
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• 제31권1호
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• pp.12-19
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• 1989

추기 된서리로 인한 자연낙엽에 관계없이 상묘를 굴취할 수 있도록 하기 위하여 1985년부터 2년간 전국 각도 9개 지역에서 9월 30일부터 11월 20일까지 10일 간격으로 6회에 걸쳐 굴취하여 묘질을 분석한 결과 다음과 같았다. 1. 묘목의 굵기는 10월 30일까지 다소의 증가를 보였다. 2. 조기굴취는 그루당 새순의 수와 길이를 떨어뜨렸으며, 10월 30일 굴취구에서 생육이 가장 양호하였다. 3. 10월 20일 이후는 자연낙엽에 관계없이 굴취하여도 활착율과 생육에 영향을 주지 않았다. 4. 굴취시기를 늦을수록 체내에 전실소와 P함량이 증가되는 반면 K는 감소하였으며 Ca는 일정하지 않았다. 5. 전실소, P, Ca 등은 뿌리껍질에서 보다 줄기껍질에 함량이 높았으며 K는 일정하지 않았다.

• Biomolecules & Therapeutics
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• 제8권3호
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• pp.255-261
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• 2000

WooHwangChungSimWon is a traditional prescription for treating with hypertension, arteriosclerosis, coma, and stroke in China, Korea, and Japan. In the new presciption of WooHwangChungSimWon, the covet is substituted for the musk, the major component of WooHwangChungSimwon, because of the prohibition law about the musk. We have made a comparative study of the effects on the ischemic damage between the musk containing and civet containing WooHwangChungSimWon. In order to investigate the effects of WooHwangChungSimWon on the ischemic damage, each samples were administrated for 12 days, ischemia was induced for 10 minutes at 7th day, and immunohistochemistry was performed in the region of hippocampus of mongolian gerbils. According to the result of immunohistochemistry, the survival rates of neuroal cells in the hippocampal CA1 region are 37.8% in the high dose of musk containing WooHwangChungSimWon (HM-WHCSW) administrated group, 27.8% in low dose of civet containing WooHwangChungSimWon (LC-WHCSW), and 35.5% in high dose of civet containing WooHwangChungSimWon (HC-WHCSW) administrated group. These survial rates were significantly different from the survival rate of sham control group (14.4%). The results suggest that all the samples except the low dose of musk containing WooHwangChungSimWon (LM-WHCSW) have protective or preventive effects on cerebral ischemia.

• 한국초지조사료학회지
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• 제15권4호
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• pp.238-244
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape(Brassica napus L.) was sown on Sep. 1, 1994. Experimental plots were divided into three replicates under 6 different cutting dates(l0 days interval from Oct. 15 to Dec. 4). Field-grown palnts were sampled on the each cutting date and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, dry matter yields were 152, 274, 500, 718, 776 and 981 kg/lOa, respectively, from the cutting date on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. Cmde protein yield significantly increased as cutting date was later until Nov. 14, thereafter a significant increase did not occured. Nitrogen and starch contents per plant significnatly increased as the cutting date was later. The increasing rate of starch was greatly higher than that of nitrogen. On the wintering period, nitrogen reserves in mts were 85.3, 68.8, 47.6, 28.3, 44.3, and 55.3 mglplant, and starch reserve were 11 1.3, 75.3, 39.3, 19.6, 26.4 and 34.6 mglplant, respectively, in the plots cut on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. It showed that carbohydrate reserves were much highly utilized than nitrogen reserves during wintering period. The rates of winter survival were 91, 83, 46, 22, 35 and 43% and regrowth yields were 692, 545, 316, 84, 127 and 140 kgD.M/lOa, respectively, in each plots. The highly significant correlation (p<0.01) between the level of organic reserves and the rate of winter survival or regrowth yield were obtained.

• 한국조경학회지
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• 제29권4호
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• pp.82-90
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• 2001

This study was carried out to reveal optimum conditions for in vitro propagation of 3 Korean native azalea species, Rhododendron mucronulatum, R. yedonese var. poukhanense, and R. shlippenbachii, which are useful for landscape proposes. Seeds and meristems from three azalea species were cultured on 1/2MS, Hyponex, and Anderson media containing growth of regulators benzyladenine(BA) and 2-isopentenyadenosine(2ip). The results were as follows. 1. In the culture of R. schlippenbachii and R. mucronulatum seeds, in vitro seedlings germinated and grew well on he 1/2MS and Anderson media, while R. yedoense var. poukhanense on Hyponex media containing 6.0mg/$\ell$ 2ip. 2. When the meristems of R. mucronulatum were cultured on Andeson media containing 9.0mg/$\ell$ 2ip, the survival rate of meristems was 23.0% in 6 weeks after culture, and the survival rate of R. schlippenbachii was 46.0% o nthe same media containing 12.0mg/$\ell$ 2ip. The survial rate of R. yedoense var. poukhanense was 92.0% onHyponex media containing 0.5mg/$\ell$ BA and 9.0mg/$\ell$ 2ip. When the meristems of R. mucronulatum and R. yedoense var. poukhanense were cultured on Hyponex media containing 12.0mg/$\ell$ 2ip, they showed the most excellent growth. R. schlippenbachii grew well on Anderson media containing 9.0mg/$\ell$ 2ip. When in vitro shoots of R. yedoense var. poukhanense were subcultured to solid medium, they grew well in shoot growth on Hyponex media containing 6.0mg/$\ell$ 2ip.

• 한국수산과학회지
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• 제33권2호
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• pp.164-170
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• 2000

Rotifer의 대량배양에는 해산 chlorella가 가장 적합하나 비용이 높고, 유지효모는 경제적이 긴 하나 먹이효율이 낮은 문제점이 있다. 이와 같은 문제점을 개선하기 위하여 본 연구에서는 광합성세균 (Rhodopseudomonas capsulata)의 첨가 효과를 조사 하였다. Rotifer 한 개체당 1일 먹이량으로 10,000, 50,000, 100,000, 200,000, 300,000세포의 Chlorella ellipsoidea를 공급하고 각 chlorella 수의 10, 20, 30배의 광합성세균을 첨가한 결과 200,000세포에 광합성 세균 20배 ($4{times}10^6 cell$)를 공급한 것이 가장 성장이 높았으나 경제적인 측면에서는 chlorella 100,000세포에 광합성세균 30배를 공급하는 것이 더 효과적이었다. 또, rotifer 1개체당 1일 먹이량으로 유지효모를 100,000, 200,000, 300,000세포를 공급하고 각 유지효모 수의 10, 20, 30배의 광합성세균을 첨가한 결과 200,000세포에 광합성세균 20배를 첨가한 실험구에서 rotifer의 성장이 가장 높았다. Chlorella와 유지효모를 각각 200,000 세포씩 그리고 chlorella와 유지효모에 광합성세균을 20배의 농도로 첨가하여 rotifer를 배양하여 넙치 자어를 사육한 결과 chlorella에 광합성세균을 첨가한 실험구의 생존율이 $96.8{\%}$로 가장 높았으며 성장의 경우도 광합성세균을 첨가한 실험구에서 높은 경향을 보였다. 또 광합성세균을 첨가한 실험구에서의 넙치 자어는 총지질과 EPA와 DHA의 함량이 광합성세균을 첨가하지 쟈은 실험구에서보다 높게 나타났다. Artemia nauplius를 6시간동안 광합성세균으로 영양강화하여 넙치 치어를 20일간 사육한 결과 영양강화한 것은 하지 않은 것보다 생존율과 성장이 높았으며 광할성세균의 최적영양걍화농도는 $ml 당 2{times}10^7 cells$로 나타났다. Chlorella와 유지 효모에 광합성 세균의 첨가는 rotifer의 성장과 자치어의 생존율과 성장에 효과적이었다. 그러나 과다한 양의 광합성세균은 오히려 negative effect가 있는 것으로 나타났다.